Combining with ancient people's experience and modern prevention and treatment on colon and rectal cancer,it views that the basic causa morbi is blocked collaterals,and the local is not reached by Yang Qi,which is also the basic mechanism of recurrence and diversion of the disease.Thereby it puts forwards "Tong(smooth) Yang" is the main discipline of the cancer;the pathogen-removing capsule made under "Tong Yang " theory displays the advantage of TCM therapy in preventing and treating colon and rectal cancer.

Objective To analyze the effect of on comprehensive therapy advanced non-small cell lung cancer,and summarize the clinical experience.Methods Based on the comprehensive therapy characteristic,we designed a clinic cases analysis database using the SQL sever database technique.Results In the 102 patients with advanced non-small cell lung cancer in the Xiyuan Hospital,the mean survival period was 11.5 months and the 1 year survival rate was 37.5%.The weight was stable and KPS was increased.There was significant difference in the improvement of mental weariness and hypodynamia,deficient breath and indolence,cough,abundant sputum,pain,anorexia,sputum mixed with blood,constipation,abdominal diarrhea and distention(P

Objective The epidemiologic evidences indicate that the benefits of using vitamin E (VE) against lung cancer remain controversial, the goal of the present paper is to know whether VE can produce the protective effect in lung cancer induced by benzo(a)pyrene B(a)P . Methods 225 Swiss mice were randomly divided into groups and treated with B(a)P and VE to systematically observe the intervention effects of VE on mouse lung cancer caused by B(a)P. Results VE exhibited no protective effect on B(a)P-induced lung cancer in female mice and instead promoted B(a)P carcinogenesis; neither protective effect nor promotion effect was observed in the male mice. The mechanism by which VE intervention influenced B(a)P carcinogenesis and lung cancer in female mice might be more complex. Conclusion The results of the present paper suggest that VE should not be used to prevent lung cancer induced by B(a)P.

Objective To explore the effect of sodium ferulate injection on myocardial function in rat model with myocardial ischemia and reperfusion. Methods Selected 60 rats aged 7 ~8 weeks (7. 26 ± 0. 36 weeks averagely) and randomly divided them into the sham group, myocardial reperfusion group, and the sodium ferulate group, with 20 rats in each group. Observed the changes of the cardiac func-tion indexes of the two groups after perfusion. Results Compared with the sham group,the degree of LVESP and ± dp/dmax decreased sig-nificantly (P<0. 05) in the myocardial reperfusion group and the sodium ferulate group. The descending degree of LVESP and ± dp/dmax 2 hours after reperfusion in the reperfusion group was significantly higher than that in the sodium ferulate group (P<0. 05). Compared with the sham group,the LVEDP of the other two groups showed a rising trend,and it was significantly lower 2 hours after reperfusion in the sodium ferulate group compared to the reperfusion group (P<0. 05). Proportion of apoptosis cells increased in the reperfusion group and the sodium ferulate group,and positive expression rate of Fas in the sodium ferulate group was significantly lower than the reperfusion group (P<0. 05). LDH and CK-MB of content in the blood of rats were significant increased,and it was higer in the reperfusion group compared with the sodium ferulate group (P<0. 05). Compared with the sham group,SOD content in the reperfusion group and the sodium ferulate group decreased obviously (P<0. 05). The MDA content increased,but the degree of increase was lower in the sodium ferulate group (P<0. 05). Conclu-sion Sodium ferulate could protect the myocardial function in rat model with myocardial ischemia and reperfusion to a certain degree.

BACKGROUND: From yiyiren oil is extracted kanglaite injection that has been extensively used against lung cancer for its function against cancer. It is revealed that kanglaite injection inhibits many strains of cancer cells including LA795 metastatic lung cancer. Genechip is used here for detection of metastasis reactive differentially expressed genes in mice that carry LA795 metastatic lung cancer.OBJECTIVE: To detect the metastasis reactive diferentially expressed genes with genechip technology in metastatic cancer carrying mice that treated with y iyiren oil injection(kanglaite injection).DESIGN: It was a controlled trial with mice as subjects.SETTING: Oncology Department of Xiyuan Hospital Affiliated to China Academy of Traditional Chinese Medicine.MATERIALS: The trial was conducted from February through August 2003. Forty mice were implanted with T739 strain lung adenocarcinoma cells and were randomly and averagely assigned into 2 groups.METHODS: mRNA probe was made from cancer sample of the mice. ScanArray 3000 scanner was used to scan the fluorescence signal in cDNA expression genechip that contained 4096 mouse genes in order to detect the differentially expressed genes.MAIN OUTCOME MEASURES: The differentially expressed genes in the two groups of mice.RESULTS: The mice genes expressed differentially between the kanglaite injection group and control group. After examination being repeated for 3 times, 27 genes presented different expression; 25 genes expression increased in both groups and 2 genes decreased. Among these differentially expressed genes, 12 genes were of the mouse muscle cDNA. It was also observed that 6 genes might be associated with the occurrence and development of tumor.CONCLUSION: Gene expression spectrum genechip is useful in effectively detect the relevent differentially expressed gene induced by kanglaite injection.

Objective To observe the growth and differentiation of the cultured mouse bone marrow stromal cells in vitro into neural like cells. Methods Bone marrow stromal cells (BMSCs) isolated from mice were cultured according to the routine method, and induced to differentiate by basic fibroblast growth factor b (bFGF), retinoic acid (RA), nerve growth factor (NGF), and dimethylsulfoxide (DMSO). Expressions of neural microfilament protein (NF 200), glial fibrillary acidic protein (GFAP), and fibronectin at 72 h after inductin were determined by immunoflurenscence and immunocytochemicalmethod. Results At 72 h after inductin, neural like changes were found in BMSCs. Immunohistochemical stain showed that 60% BMSCs differentiated into NF 200 positive cells, and about 50% BMSCs into GFAP positive cells. Fibronectin expression was significantly lower as compared with that in the control group. Conclusion Combined action of RA, bFGF, NGF, and DMSO can induce BMSCs to differentiate into neural like cells in vitro .

Objective To study the clinical use of intramedullary interlocking nail in treatment of femoral and tibial fractures. Methods104 cases of femoral or tibial fractures were treated with intramedullary interlocking nails.Results The close and open reduction fractures had healed 6 10 and 8 12 weeks after surgery, and the fixation nails were taken out after 48 weeks. No broken nail, no delayed or nonunion, no infection, no fat embolism, no stiff joint occurred, All cases were followed up and the average following was 20 months without fracture.Conclusion The intramedullary interlocking nail had obvious advantage of reliable fixation, rotation and separation prevention, no need of external fixation support, high rate of fracture healing and allowance of early walk without support.

Objective Using zebrafish to analyze the effect of water temperature on the recovery of spinal cord in-jury. To detect the cell proliferation and changes of gene expression at the injury site during the process of recovery. Meth-ods Surgical operation was performed to induce spinal cord injury ( SCI) on adult fish. Water at a series of temperature was applied to culture the fish. Swimming ability was adopted to observe the recovery of spinal cord injury following surger?y. Vibration sections and immunohistochemistry were performed to observe the cell number post SCI at different stages. The changes of gdnf and nos gene expression were determined by real?time PCR. Results The water temperature changes from 28℃ to 32℃ did not affect the swimming ability of non?injured and sham?injured fish ( P>0. 05 ) . The swimming ability recovered mostly in 8 weeks post spinal cord injury. At 32℃, the swimming ability recovered faster than at 28℃ or at 30℃(P<0. 05). The cell proliferation increased obviously following spinal cord injury (P<0. 05). The proliferation of cells surrounding the spinal cord in jury was more extensive in SCI fishes incubated in 32℃ water than in 28℃ or 30℃ water ( P<0. 05). Real?time PCR assay showed that gdnf was up?regulated in all groups post SCI at 24 h, and 7 and 14 days (P<0. 05). The nos expression was up?regulated in all groups following SCI in 24 h (P<0. 05) and 7 days. There was no sig?nificant difference between the SCI group and sham?injury group (P<0. 05), while after 14 days, the expression of nos was reduced in the SCI group compared with the sham?injury group (P<0. 05). Conclusions A slight increase of incu?bating water temperature can accelerate the recovery of spinal cord injury in zebrafish.

ObjectiveTo construct human embryonic kidney cells (HEK293) modified with human preproenkephalin (hPPE) gene.MethodshPPE gene fragments were obtained from recombinant plasmid pcDNA3.1( + )/hPPE by using restriction endonuelease Hind Ⅲ and Not Ⅰ.Homologous recombination of lentivirus and hPPE gene was produced by using recombinant DNA technology.HEK293 cells were then transfected with the recombinant lentivirus vectors.The expression of hPPE gene in HEK293 cells was detected by Western blot.ResultsThe results of DNA sequencing indicated that the positive clone of recombinant lentivirus was completely consistent with sequencing of hPPE in Genebank.The titer of the concentrated virus was 2.07 × 108 TU/ml.GFP fluorescence was not seen in HEK293 cells transfected with the lentiviral vector under fluorescence microscope.A strong fluorescence was seen in HEK293 cells transfected with Ubc-GFP-L.V.empty viral vector.Positive expression of hPPE was demonstrated in HEK293 cells transfected with lentiviral vector by Western blot.Conclusion HEK293 cells modified with hPPE gene were successfully constructed and the target gene hPPE was stably expressed in HEK293 cells.

Objective To explore the mechanism and effects of basic fibroblast growth factor( bFGF)on skin wound healing. Methods Fibroblasts( FB)were isolated from normal skin and hypertrophic scar and cultivated by direct adherence method. FB were then treated with different concentrations of bFGF(0,0. 1,1,10,100,1 000 ng·mL-1 )and cultivated with serum-free medium for 72 hours. The proliferation and apoptosis of FB in each group were detected by cell counting and trypan blue staining. Content and gene expression of typeⅠand type Ⅲ collagen and fibronectin were determined by ELISA and RT-PCR,respectively. Results bFGF promoted the proliferation of FB at low concentrations promoted apoptosis of FB at higher concentrations. The proliferation of FB from hypertrophic scar was slower than that from the normal skin. bFGF significantly inhibited type Ⅰ collagen production from hypertrophic scar FB but not from the normal skin. Moreover,bFGF up-regulated fibronectin expression in the normal fibroblasts,but not in the hypertrophic scar. No change in type Ⅲ collagen expression and production was observed in FB from either source. Conclusion bFGF has differential effects and mechanisms on FB of the normal skin and hypertrophic scar,suggesting that bFGF may play a role in early phase of skin wound healing and scar formation.