By interpreting the connotation of physical factors and its significance in the syndrome differentiation of traditional Chinese medicine (TCM), ideas and methods of the combination of physique differentiation and syndrome differentiation of coronary heart disease (CHD) were proposed. The physique classification research was promoted and standardized. Research methods of physique differentiation were improved. The connotation of relationship between constitution and syndrome was mined. The essence of different syndromes was explored on different syn-dromes of CHD. The physique differentiation, disease differentiation and syndrome differentiation were integrated with the viscera-state theory and syndrome factor differentiation theory. The difference of proteomics technology was used as the means to reveal the pathogenesis of same disease with different syndrome of different CHD constitution. Multidisciplinary crossover study was developed on CHD with constitution theory of TCM and clinical actively, so as to make it better to direct the clinical scientific research practice of CHD.

This article was aimed to study the distribution and effects of apolipoprotein E (ApoE) gene polymorphism of different constitutional types among coronary heart disease (CHD) patients with blood stasis syndrome on vascular endothelial cell (VEC) function. The whole gene sequencing method was used to identify genotypes of ApoE gene among 556 CHD patients with blood stasis syndrome, in order to analyze the relationship between polymorphism of ApoE gene and the level of VEC function. The results showed that the frequency of E3/3 genotype of each physical group was significantly lower than that of the healthy group; and the frequency of E3/4 genotype was significantly higher than that of the healthy group. In addition, the frequency of E3/3 genotype in qi deficiency constitution group was higher than that of blood stasis constitution group and yang deficiency constitution group; but the frequency of E3/4 genotype in blood stasis constitution group and yang deficiency constitution group was higher than that of the qi deficiency constitution group. The levels of ET and ET/NO in each of genotype groups of blood stasis constitution, yang deficiency constitution, qi deficiency constitution of CHD patients with blood stasis syndrome were higher than that of the healthy group (P < 0.05). In the genotype group of blood stasis constitution of CHD patients with blood stasis syndrome, the frequency of E3/4+E4/4 genotype ET was higher than that of other genotypes (P < 0.01). The levels of ET, NO, ET/NO in genotype groups of yang deficiency constitution and qi deficiency constitution of CHD patients with blood stasis syndrome were not significantly different. It was concluded that ApoE genotype E3/4, E3/3 may be the susceptible genotypes of blood stasis syndrome in CHD. There is a certain difference among different constitutional types. CHD with blood stasis syndrome patients, who are the constitution of blood stasis, carrying the ApoE gene polymorphism of E4 allele may have the function of increasing the ET level.

Objective To study the distribution of body constitutions of CHD patients with blood stasis syndrome;To explore the relationship between different body constitutions and their blood lipid levels.Methods WANG’s Constitutional Classification was used to diagnose body constitutions of 600 CHD patients with blood stasis syndrome, and analyze the relationship between the different body constitutions and triglyceride (TG) level, low density cholesterol (LDL-C) level, high density cholesterol (HDL-C) level.Results The four most common body constitutions of CHD patients with blood stasis syndrome in Taiyuan area were the constitutions of blood stasis, yang deficiency, qi deficiency and yin deficiency. The TG levels of the four body constitutions were higher than those of healthy people (P<0.01), but there was no obvious difference among TG levels of different body constitutions. The LDL-C level of the patients with the body constitution of blood stasis was higher than that of patients with other body constitutions and healthy people (P<0.01). Compared with healthy people, there was no obvious difference among HDL-C levels of different body constitutions (P>0.05).Conclusion There is a certain difference among the blood lipid levels in different body constitutions of CHD patients with blood stasis syndrome, and the patients of blood stasis syndrome with high LDL-C level are more dangerous than patients with other body costitutions.

Gynura divaricata polysaccharide was extracted from Gynura divaricata with the method of microwave-assisted extraction. Based on the results of single factor experiments, the extraction process was optimized by response surface analysis. The results indicated that the influencing factors during extraction were: liquid-solid ratio, microwave time, and microwave power, among which the influence of liquid-solid ratio was the most significant. The optimal conditions were as follows: liquid-solid ratio of 45∶1(mL/g), microwave power of 390 W, microwave time of 22 min. Under optimal extraction conditions, the extraction rate of Gynura divaricata polysaccharide was 15. 36%, which was pretty close to the predicted result. The optimized response surface methodology had some practical value for the process.