2.RESEARCH ON THE GLYCEMIC INDEX AND INSULIN INDEX OF DIFFERENT CARBOHYDRATE FOODS
Wenqing ZHANG ; Yueming ZHANG ; Yuexin YANG
Acta Nutrimenta Sinica 2004;0(06):-
Objective:The glucemic index(GI)and insulin index(II)of wheat flour, oat fiber and resistant starch (RS) were studied.Method:Ten healthy subjects consumed 4 kinds of carbohydrate foods: glucose powder,wheat flour food, oat fiber food and RS food.Up to 120 min after consumption of test materials, blood samples were taken for glucose and insulin analyses.Results:The GI value of wheat flour food, oat fiber food, RS food was 88.24?20.84,60.16?14.16,47.05?10.22 respectively,regarding glucose powder as 100 . The II value of them was 83.06 ? 10.68 , 68.32 ? 17.08, 60.26 ? 30.1 respectively.The area and peak level under the glucose-response curve and insulin-response curve of RS food were significantly lower than those of glucose powder and wheat flour food (P
3.Estimation of stature by length of stemal-pubisline and cerivial vertebrae-coccyx
Zhi LI ; Yuexin MU ; Dafu YANG
Chinese Journal of Forensic Medicine 2002;0(06):-
Objective To study relationship between length of sterna-pubis line, cervical vertebrae-coc-cyx line and stature in West of Hubei Province. Methods 400 men and women were randomly chosen. Length of sterna-pubis line, length of cervical vertebrae-coccyx line and stature in every person were mea-sured, and all data analysesd by multiple linear regression analysis. Results we have estiblished Eight quadratic equation which stature was calculated via different sex and age groups. Significant difference was observed between the length of sterna-pubis line, and cervical vertebrae-coccyx line and the stature in age group of 18-40. Yet nosignificant difference was seen over 41 and this method must be used with care. Conclusion These equations can be used to determine the stature of Chinese adults.
4.DETERMINATION OF BIOTIN IN FOODS AND FEEDS
Lei SHI ; Xiaoli YANG ; Guodong WANG ; Yuexin YANG ;
Acta Nutrimenta Sinica 1956;0(02):-
Objective: To establish microbiological method for determining biotin in foods and feeds. Methods: Using biotin dependent microorganisms Lactobacillus plantarum(ATCC 8014), the biotin content was detected indirectly by the growth of cultrued bacteria spectrophotometrically.Results: The detection limit was 0.03 ng. The relative standard deviations(RSD) of within and between run assays were 2.2%-3.8%,2.1%-5.3% respectively. The recovery of added biotin was 93.4%-104.6%, and RSD was 3.1%-4.1%. Conclusion: This assay is sufficiently accurate and repoducible for determining biotin in foods and feeds.
5.Expression of the Four Mismatch Repair Genes Protein of Patients with Colorectal Cancer and Its Clinical Significance
Xuexian TAN ; Yuexin YANG ; Qiuping LUO ; Wen YANG ; Qingping JIANG
Progress in Modern Biomedicine 2017;17(23):4514-4517
Objective:To analyze the expression of the four mismatch repair genes protein (hMLH1,hMSH2,hMSH6 and hPMS2) of patients with colorectal cancer and its clinical significance.Methods:177 cases of patients with colorectal caner in the Third Affiliated Hospital of Guangzhou Medical University from January 2013 to December 2015 were randomly selected.Tested the expression of the hMLH1,hMSH2,hMSH6 and hPMS2 by immunohistochemistry,the relationship between protein expression and clinical parameters was analyzed.Results:Among 177 cases ofcolorectal cancer tissue,the deletion rate ofhMLH1 protein was 6.2% (11/177),the deletion rate of hMLH2 protein was 4.0%(7/177),the deletion rate of hMSH6 protein was 1.7%(3/177),the deletion rate of hPMS2 protein was 8.0%(14/177),the sum of the four values accounted for 19.8%(35/177) of all cases of colorectal cancer.The loss of expression of the four mismatch repair genes protein were correlated to tumor location (P<0.05),besides,the loss of expression of the hMLH1 and hPMS2 protein were correlated to degree of tumor differentiation (P<0.05),he loss of expression of the hMSH6 protein were correlated to depth of tumor invasion(P<0.05);But the loss was not correlated to age,sexes,lymph node metastasis and distant metastasis(P>0.05).Conclusion:The expression of loss phenomenon with mismatch repair protein appears in part of colorectal cancer,the loss phenomenon with mis match repair protein were correlated to tumor location and degree of tumor differentiation.Mutations of four genes in hMLH1,hPMS2,hMSH6 and bMSH2,to provide a reference value for the clinical judgment of prognosis and to develop a treatment plan.
6.Acceptance of knowledge to food glycemic index and dietary adjustment in diabetic patients☆
Hongwei WANG ; Yuexin YANG ; Yinfa ZHANG ; Junhua HAN ; Zhu WANG
Chinese Journal of Tissue Engineering Research 2007;11(52):10701-10703
BACKGROUND: Glycemic index (GI) is a physiological index of carbohydrate food. It has been found out in epidemiological studies that GI is useful in controlling and preventing some chronic diseases such as diabetes,cardiovascular disease,obesity.OBJECTIVE:To investigate the acceptance and practical significance of GI knowledge in diabetic patients by comparing with the traditional education with food exchange list (FEL)as the main material.DESIGN:A comparative observational test.SETTING:Institute of Nutrition and Food Safety,Chinese Center for Disease Control and Prevention.PARTICIPANTS: Seventy-two diabetic patients volunteered to join in the study were selected from the Department Endocrinology of Beijiog No.6 Hospital from October 2000 to February 2002,including 36 males and 36 female of 10-70 years old with an average age of 58 years.METHODS:The 72 diabetic patients were randomly assigned to two groups:① GI group which received GI-based nutrition education;② FEL group receiving FEL-based nutrition education.They were educated for 5 months by means of classes,personal education and consultation by telephone.The changes of knowledge,food selection and blood glucose in the diabetic patients were observed before and after education.MAIN OUTCOME MEASURES:Changes of the awareness of nutrition education,fasting blood glucose (FBG) and 2-hour postprandial blood glucose (2hPBG) before and after education.RESULTS: All the 72 diabetic patients were involved in the analysis of results.①The correct answering rates after education were obviously higher than that before education (P<0.01).Before education,the correct rates of answers to GI questions in the Gl group were lower than the correct rates of answers to FEL questions in the FEL group (0 vs.6.5%,P<0.01).After education,the correct rates in the Gl group were higher than those in the FEL group (92.2%VS.79.4%,P<0.01): whereas there were no significant differences in the average correct rate of answer to the same question between the two groups (P>0.05).The Gl group was better than the FEL group in selecting cereal,fruit,bean and its.products (P<0.01).② The FBG and 2hPBG after education were lower than those before education in both groups(P<0.01),whereas there was no significant difference between the two groups (P>0.05).CONCLUSION:The knowledge of GI was better accepted than that of FEL by the diabetic patients in dietary treatment.
7.Clinical significance of human telomerase RNA component and C-myc gene expression in cervical cancer and precancerous lesions
Hanzhen XIONG ; Yuexin YANG ; Zhongtang XIONG ; Shaoyan LIU ; Qingping JIANG
Cancer Research and Clinic 2012;24(8):508-511
Objective To investigate the expression level of human telomerase RNA component (hTERC) and C-myc in cervical lesions.Methods Fluorescence in situ hybridization (FISH) was applied to detect hTERC and C-myc expression in 62 cases of cervical tissue including 35 cases of cervical intraepithelial neoplasia,8 cases of invasive cervical cancer,19 cases of inflammation as controls.Results The positive rates of hTERC on chromosome 3 in chronic cervicitis organizations,CIN Ⅰ,CIN Ⅱ-Ⅲ and cervical cancer were 5.3 % (1/19),16.7 %(2/12),87.0 % (20/23),87.5 % (7/8) (x2 =36.299,x2 =40.237,P <0.01).The positive rates of C-myc in chronic cervical inflammation organization,CIN Ⅰ,CIN Ⅱ-Ⅲ and cervical cancer were 5.3 % (1/19),8.3 % (1/12),78.3 % (18/23),62.5 % (5/8) (x2 =30.200,x2 =34.224,P <0.01 ).The differences among groups were statistically significant.hTERC had a positively correlation with C-myc expression (r =0.514,P < 0.01).Conclusion The expression of hTERC and C-myc on chromosome 3 is closely related to cervical cancer progression.
8.Test of validity and reliability of Nursing Professional Values Scale in registered nurses
Yuexin LIU ; Yuying CHEN ; Yiwei LI ; Xiao YANG ; Lingling GAO
Chinese Journal of Practical Nursing 2013;29(33):47-50
Objective To explore the validity and reliability of Nursing Professional Values Scale in registered nurses.Methods Demographic questionnaires and the Revised Nursing Professional Values Scale were used among 280 registered nurses.Results The Cronbach's α was 0.94.The factor analysis extracted 4 factors which could account for 59.55% variances.These four factors were defined as professional characteristic,providing care,behaviorist,and trust.The Cronbach's α for these four factors were 0.89,0.87,0.86 and 0.70 respectively.Conclusions The Revised Nursing Professional Values Scale possesses desirable validity and reliability in registered nurses,and is a psychometric good instrument of professional value.
9.The preventive effect of acarbose on the imapired glucose tolerance population from developing diabetes and cardiovascular diseases
Yuexin WANG ; Yang ZHANG ; Jiang WU ; Fengping ZHANG
Chinese Journal of Endocrinology and Metabolism 1986;0(04):-
In this study, 116 subjects with impaired glucose tolerance were followed up for 3 years. The results showed that the incidences from diabetes and coronary heart disease in acarbose treatment group were less than those in the control group.
10.ANALYSIS OF PHYTOSTEROLS CONTENT IN CEREALS AND LEGUMES
Junhua HAN ; Meiyuan FENG ; Guodong WANG ; Yuexin YANG
Acta Nutrimenta Sinica 2004;0(05):-
Objective: To analyze the phytosterols content in cereals and legumes commonly consumed in China, and to estimate the intake of phytosterols in Chinese people. Methods: 25 cereals and 16 legumes were chosen as samples. The content of ?-sitosterol, campesterol, stigmasterol, ?-sitostanol, campestanol were analyzed by GC methods and the total phytosterols were calculated; The intake of phytosterols in Chinese people were estimated using the data of “Survey on the Status of Nutrition and Health of the Chinese People” in 2002. Results: The content of phytosterols in wheat flour was higher than that in rice. The refinements of cereals will decrease the phytosterols content. Phytosterols content in soybean was higher than other legumes. The intake of phytosterols in Chinese people was estimated to be 130.76mg/d from cereals and 7.86mg/d from legumes. Conclusion: The analysis of phytosterols in food and the estimation of daily intake is valuable for chronic disease prevention. The higher consumption of wheat flour and legumes in diet is recommended.