1.The correlation between vascular cell adhesion molecule-1 and gastric carcinoma
Chinese Journal of Current Advances in General Surgery 1999;0(04):-
To detect the expression of VCAM 1 (vascular cell adhesion molecule 1)in gastric cancer,its adjacent tissues, gastric ulcer and normal tissues, and the correlation of expression of VCAM 1 with tumor growth,metastasis, pathological parameters.Methods: Specimens from 41 gastric cancer patients(cancer, adjacent cancer and normal tissue),8 peptic ulcer,10 normal peoples were stained, image analysis with the methods of immunohistochemistry.The relation of the data collected with clinic and pathology of gastric cancer was studied. The correlation of VCAM 1 expression and pathological characteristics of gastric cancer were studied.Results: VCAM 1 was expressed not only in vascular endothelial cells, but also in most gastric cancer cells, thirty one out of forty one cancer had the expression of VCAM 1,adjacent cancer(2cm) had 5 positive VCAM 1 and adjacent cancer(5cm) had 4 positive VCAM 1(P
2.The analysis of relation of the ratio and number of perigastric lymph node to prognosis of patients with gastric carcinoma
Yongbin DING ; Yuexian LIU ; Guoy CHEN
Chinese Journal of Current Advances in General Surgery 1998;0(01):-
Objective:To study the correlation of the ratio and number of involved lymph nodes and the prognosis of gastric carcinoma.Methods: 174 patients of positive gastric lymph nodes were included.They were treated by radical gastrectomy and lymph nodes dissection(D 2 or D 3)in our department.The ratio and number of metastastic gastric lymph nodes were calculated.Their influence and their interrelation with pathogenic parameters were analyzed.Results: The overall 5-year survival rate was 29.6%,and the five-year survival rate of 1~20 per cent and more than 20% were 70.6%,12.0% respectively.One to five positive lymph nodes and five more were 46.6%,17.4% respectively.The five-year survival rate was decreased as the ratio and number of involved lymph nodes increased.Studies also indicated that with the increase of depth of cancer invasion,the ratio and number of involved lymph node elevated.Conclusion: The results shows that the ratio and number of involved lymph nodes correlated to tumour progression and the five survival rate.Perigastric lymph node status can be considered as a simple and effective prognosis factor.
3.Reform on teaching mode of battlefield first aid based on the traits of the medics
Yuexian DENG ; Wenbao LIU ; Lei JIANG ; Jianjun ZHAO ; Chao QIN
Chinese Journal of Medical Education Research 2013;(6):600-602
Medics play a very important role in military medical supporting forces who take on lots of basic work of medical units.It was assigned to us to train the medics for the first time this year in our university.To enhance the teaching effect of the course named first aid in battlefield,a series of measures were taken including modularizing the teaching contents,diversifying the teaching means,dividing students into some groups,strengthening the practice and examinations based on the traits of the medics and so on.At last,some countermeasures were put forward aiming at the process of teaching.
4.Influence of comprehensive nursing intervention on quality of life and activity of daily living in community patients with convalescent stroke
Aihong WANG ; Guihua XU ; Qiudi WU ; Yuexian LIU ; Xinru GE
Chinese Journal of Practical Nursing 2010;26(8):11-14
Objective To study on the influence of comprehensive nursing intervention on extremities function and quality of life (QOL) in community patients with convalescent stroke.Methods A total of 200 community patients with convalescent stroke were selected and randomized into the experimental group and the control group,100 cases each.Patients in the control group were given conventional treatment and following.While patients in the experimental group were given comprehensive nursing intervention besides the treatment mentioned above.Then the therapeutic effects were evaluated by filling out brief Fugl-Meyer assessment(FMA) scale and ADL rating scale(FIM).Life satisfaction index A(LSIA) and quality of life index (QLI) were selected to evaluate QOL.Results After treatment,the scores of LSIA and QLI of the control group showed no difference,but these scores of the experimental group were significantly improved,and was better than those of the control group.In addition,both FMA and FIM in these two groups improved obviously compared to those before treatment and the effect of the experimental group was superior to that of the control group.Conclusions The comprehensive nursing intervention can significantly improve extremities function and ADL and QOL of community patients with convalescent stroke.It is economical,simple and suitable to community families with any education background.
5.Effectiveness of blending learning in teaching of Fundamental of Nursing
Haiyan YIN ; Aihong WANG ; Yuexian LIU ; Yayuan DING ; Guirong HE
Chinese Journal of Practical Nursing 2014;30(23):76-78
Objective To discuss the effectiveness of implementation of blending learning in teaching of Fundamental of Nursing.Methods 234 nursing undergraduates were divided into the experimental group and the control group.The experimental group adopted blending learning method,while the control group used traditional teaching method.The final term exam and technical test results were compared between two groups.Results Students' practice scores in the experimental group were higher than those of the control group.In the experimental group,about 90.2% undergraduates accessed learning resources through online teaching platform,82.4% students considered that their nursing skills improved and 78.4% students thought that their self-learning capacity strengthened.Conclusions Blending learning gives students a potential time,environment and recourse to learn.By integrating in-class and after-class studying,undergraduates' self-learning ability and nursing skills improved.
6.Discussion on the Building of Teacher Staff on Post-oriented Education in Army
Yuexian DENG ; Guoliang CHENG ; Chao QIN ; Wenbao LIU ; Lunlun HANG
Chinese Journal of Medical Education Research 2003;0(02):-
With the development of world new military revolution,the objective of education has transformed from degree-oriented to post-oriented in military university.Based on status-on-quo investigation,the author finds some deficiencies in building of teacher staff and put forward some measures.
7.The role of histone H3 acetylation on cleft palate in mice induced by 2, 3, 7, 8-tetrachlorodibenzopdioxin .
Liu CUIPING ; Yuan XINGANG ; Fu YUEXIAN ; Qiu LIN ; Tian XIAOFEI ; Liu YAN ; Wei GUANGHUI
Chinese Journal of Plastic Surgery 2014;30(5):369-372
OBJECTIVETo explore the role of histone H3 acetylation in cleft palate induced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin (TCDD) in C57BL/6J mice, and its mechanism.
METHODSOn gestation day 10 (GD10), 36 pregnant mice were randomly divided into two groups as the treated group(n = 18) and the control group( n = 18). The mice in the treated group received intragastric administration with TCDD 28 μg/kg, while the mice in the control group received equivalent corn oil. The pregnant mice were sacrificed on GD13. 5, GD14. 5 and GD15. 5, collecting fetal palates to determine the activities of histone acetyltransferases (HATs) by Colorimetric and the expression level of acetylated histone H3 (Acetylated histone H3, Ac-H3) by Western-blot.
RESULTSThe activity of HATs was 0.409 7 ± 0.0147, 0.522 3 ± 0.017 1 and 0.643 5 ± 0.013 9 in control group on GD13.5, GD14.5 and GD15.5; 0.865 0 ± 0.0129, 0.719 1 ± 0.017 8 and 0.551 2 ± 0.016 8 in TCDD group. The activity of HATs in TCDD group was higher than that in control group on GD13. 5, GD14. 5, showing significantly difference between the two groups (t = - 56. 932, t = - 19. 516, P < 0.01); however, the activity of HATs in TCDD group was significantly lower than that in control group on GD15. 5 (t = 10. 382, P < 0.01). The expression level of Ac-H3 was 0.745 0 ± 0.113 5, 1.055 9 ± 0.249 4 and 1.795 5 ± 0.081 9 in control group on GD13. 5, GD14. 5 and GD15. 5; while 1.4490 ± 0. 1460, 1. 641 8 ± 0.099 7 and 1. 512 1 ± 0. 150 2 in TCDD group. The expression of Ac-H3 in TCDD group was higher than that in control group on GD13. 5, GD14. 5, showing significantly difference( t = -6. 593, -3. 779, P <0. 01, P <0.05) ; However, the expression of Ac-H3 in TCDD group was statistically lower than that in control group (t = 2. 870, P <0. 05).
CONCLUSIONThe acetylation of histone H3 was involved in the cleft palate of C57BL/6J mice induced by TCDD, which may be one of the mechanisms in TCDD-induced cleft palate.
Acetylation ; drug effects ; Acetyltransferases ; metabolism ; Animals ; Cleft Palate ; chemically induced ; metabolism ; Dioxins ; Female ; Fetus ; Histones ; metabolism ; Humans ; Mice ; Mice, Inbred C57BL ; Polychlorinated Dibenzodioxins ; Pregnancy ; Random Allocation ; Teratogens
8.Reconstruction finger web with dorsal two wing-shaped flap for the treatment of congenital syndactyly.
Xiaofei TIAN ; Lin QIU ; Yuexian FU ; Yan LIU ; Xinguang YUAN ; Jun XIAO ; Tianwu LI
Chinese Journal of Plastic Surgery 2014;30(2):96-98
OBJECTIVETo explore the clinical effect of using dorsal two wing-shaped flap to reconstruct finger web for treatment of congenital syndactyly.
METHODSThis technique has been used in 19 children with congenital syndactyly. At the dorsum, a flap with V-shaped tip and two wing-shaped pedicle were designed and was just sewed up with an anchor-shaped incision at the palm. The web was primarily reconstructed without skin graft at base of fingers. Distal end of fingers were separated by using serrated flap and were closed after removal of fatty tissue. At some cases with tight skin connection. The defect area at lateral and distal end of fingers was closed by small pieces of skin graft.
RESULTSAll the webs were reconstructed primarily without skin graft at the base of fingers. 7 cases with tight skin connection had small pieces of skin graft at lateral and distal end of fingers. Primary healing was achieved in all cases. After 1 to 6 months of follow-up, both the appearance and function were satisfactory without conspicuous scar. The reconstructed finger webs were in normal depth and width.
CONCLUSIONSPrimary web space can be achieved by dorsal two wing-shaped flap without skin graft at base of fingers. It is one of the best choices for treatment of congenital syndactyly.
Adipose Tissue ; surgery ; Child ; Cicatrix ; Dermatologic Surgical Procedures ; methods ; Fingers ; surgery ; Humans ; Skin Transplantation ; Surgical Flaps ; transplantation ; Syndactyly ; surgery ; Wound Healing
9.New biological dermis cover Pelnac? in wound healing of children with severe trauma
Liang TIAN ; Lin QIU ; Yuexian FU ; Xiaofei TIAN ; Yan LIU ; Jun XIAO
Chinese Journal of Tissue Engineering Research 2014;(16):2601-2606
BACKGROUND:In recent years, tissue engineering has made great progress, and skin tissue engineering is especialy noteworthy. Artificial dermis (PELNAC) is relatively used widely, but there is a lack of relevant reports on wound repair in children. OBJECTIVE:To investigate the clinical efficacy of Pelnac? METHODS:In a retrospective study, 22 patients with the wound of severe trauma were treated with Pelnac as skin graft dressings on treatment of the wounds of severe trauma in children. ? graft, negative-pressure wound therapy and split-thickness skin graft as experimental group (Pelnac? group), and another 19 patients treated with granulation formation dressing and split-thickness skin graft as control group. We colected data including the graft livability, the required re-operative times and the epithelization time after the skin graft. During the folow-up, the skin color and texture of survival skin, subcutaneous fulness, scar hyperplasia and the joint function were also evaluated. RESULTS AND CONCLUSION: In the Pelnac ? group, the graft livability was up to 90% within 10-14 days after grafting. The secondary split-thickness skin graft was required in two cases in the Pelnac? group and in eight cases in the control group. There was a significant difference in the graft livability (P < 0.05). The average epithelization time after the skin graft was (13.86±3.09) days in the Pelnac? group, which was significant shorter than the control group, (19.10±4.62) days, after the first time operation (P< 0.05). During the 10 months folow-up, the survival skin color and skin elasticity in the Pelnac? group was significantly better than that in the control group (P< 0.05). Better subcutaneous fulness and milder scar hyperplasy in the injured sites were obtained in the Pelnac? group compared with the control gorup. Five cases had certain joint function limitation in the Pelnac? group, compared to 10 cases in the control group, with a significant difference (P < 0.05). Artificial dermis Pelnac? has a stronger anti-infectious ability and higher graft livability. Pelnac? graft combined with negative-pressure wound therapy, granulation culture and split-thickness skin graft can shorten the epithelization time, improve wound healing and aleviate harm to the joint function after the skin graft.
10.Expression of GABAB1 receptors in spinal dorsal horn neurons in rats with diabetic neuropathic pain
Yantao LIU ; Xiuli WANG ; Qian WANG ; Rui DONG ; Jianghong MA ; Qiujun WANG ; Yuexian GUO
Chinese Journal of Anesthesiology 2010;30(10):1216-1219
Objective To investigate the role of GABAB1 receptors in spinal dorsal horn neurons in the development of diabetic neuropathic pain (DNP). Methods Sixty pathogen free male SD rats aged 4 weeks weighing 150-170 g were randomly divided into 2 groups ( n = 30 each): control group and DNP group. Diabetes mellitus was induced by single intraperitoneal injection of streptozotocin 50 mg/kg. Blood glucose levels and paw withdrawal threshold to mechanical stimuli were measured at 3, 5 and 7 weeks (T1, T2, T3 ) after IP STZ/NS ( n = 10 each). The animals were sacrificed after PWL measurement. The lumbar segment of the spinal cord was removed for determination of the expression of GABAB1 receptors by immuno-histochemistry, RT-PCR and Western blot analysis. Results The blood glucose levels were significantly higher while the PWT was significantly lower at T1,T2 and T3 in group DNP than in control group. The expression of GABAB1 receptor mRNA and protein in spinal dorsal horn was significantly lower at T2 and T3 in DNP group than in control group. Conclusion The expression of GABAb1 receptors is down-regulated in spinal dorsal horn neurons in rats with DNP.