Objective To discuss the association of allele polymorphisms SNP-rs1799724(C>T)in the TNF-αand SNP-rs11559013(G>A)in the ALCAM with HCV chronic infection in Han population in Yunnan province. Methods 434 HCV chronic infectious patients and 444 healthy individuals of Han Chinese population in Yunnan province were recruited. Two single nucleotide polymorphisms(SNPs)in the SNP-rs1799724(C>T) of TNF-αgene and SNP-rs11559013(G>A)of ALCAM gene were determined by real-time TaqMan polymerase chain reaction. We evaluated the associations of the two SNPs with HCV chronic infection. Results The distributions of allele and genotype of SNP-rs1799724(C>T)in the TNF-αand SNP-rs11559013(G>A)in the ALCAM between hepatitis C virus(HCV)chronic infectious patients and the healthy controls were not statistically significant(P > 0.05). Conclusion SNP-rs1799724(C>T)in the TNF-αand SNP-rs11559013(G>A) in the ALCAM have no association with HCV chronic infection in the Han population in Yunnan province.

OBJECTIVE:To establish the quality standard for Gaultheria yunnanensis. METHODS:TLC was adopted for quali-tative identification of samples. Moisture,total ash and acid-insoluble ash were determined. HPLC method was used to determine the content of methyl salicylate. The determination was performed on Eclipse XDB-C18 column with mobile phase consisted of meth-anol-water(62:38,V/V)at the flow rate of 1.0 mL/min. The detection wavelength was set at 307 nm,and column temperature was 30 ℃. The sample size was 10 μL. RESULTS:TLC spots of samples were clear and well separated. The moisture was 8.2%-10.8%,total ash was 0.9%-4.0% and acid-insoluble ash was 0.1%-0.9%. The linear range of methyl salicylate were 0.045-0.73μg(r=0.9999). RSDs of precision,stability and reproducibility tests were no more than 1.0%. The recoveries of meth-yl salicylate were 97.8%-104.3%(RSD=2.6%,n=9). CONCLUSIONS:The established standard can be used for quality control of G. yunnanensis.