Twenty-eight moderately halophilic bacteria isolated from salt lakes in Xinjiang Regions were analyzed by 16S rDNA PCR-RFLP in comparison with nine reference strains of related species. The 16S rRNA PCR products of the tested stains were digested by four restriction enzymes (AluI, HinfI, RsaI and HaeⅢ). The results showed that all tested strains were clustered into three groups at 74% similarity level. Group I included 2 reference strains Chromohalobacter marismortui, Nesterenkonia halobia and new isolate CI. Group Ⅱ included seven type strains belonging to Halomonas and eight new isolates. Group Ⅲ included ninteen new isolates.