Nutritional therapy is an important part of therapy of chronic diarrhea in children. The nutritional status is very important to growth and development of the children patients. The supplementation of appropriate amount of protein,abundant energy,vitamin,minerals and probiotics can improve the nutrition and promote growth and develop-ment,then remission the development of disease and decrease the incidence of the complication.

Giardia lamblia is an important intestinal protozoan which can cause diarrhea in humans. The detection of Giardia infection is performed through the detection methods of pathogen，immunoassay and molecular biology. Currently，the immunodiagnostic methods have good application and development prospect because of high sensitivity and specificity，simple and convenient，and time saving. In this article，we review the main progress and application of immunodiagnostic methods for Giardia infection.

Objective advance“equal pay for equal work” standard for contract nurses,and approach for effective means of stabilizing nursing staff.Methods in order to improve of nursing staff's ideological understanding,personnel department and nursing department utilize their advantages of political work in the the Communist Party,the Communist Youth League and the Labor Unions,and bridge the cap between contract and staff nurses in clinical department in terms of welfares and promotion to realize the goal of “equal pay for equal work”.Results all contract nurses join the Labor Union.contract nurses play active role in the Commtmist Youth League.Salaries,insurance and other welfares for contract nurses are guaranteed.Contract nurses enjoy equal opportunity of promotion in technical positions and of open recruitment as registered nurses.Conclusion treatment disparity has been effectively alleviated between contract and staff nurses,so that the nursing staff becomes stable.

Objective To explore the value of low resection of rectal carcinoma in pull-through anus operation and its influence factor with rectal carcinoma distant metastases along intestine wall.Method Clinical pathological and visited data of 43 patients with rectal carcinoma were analyzed prospectively.Resuits Forty--one cases were successfully accepted resection in pull-through anus (95.3%).No patient suffered from stoma leak,3 cases appeared recurrence after operation.Compared between high differentiation patients and mid differentiation patients,there was no significant difference, while the difference of comparing between high or mid differentiation patients and low differentiation patients respectively,there was significant difference.The difference of comparison among Dukes stage A,B or C was significant.Conclusions The right length of incising distant intestine tube is more than 2.0,3.0,4.0 cm respectively for rectal careinoma of high,mid and low differentiation or that of Dukes stage A,B or C.low resection of rectal carcinoma in puB-through anus should be considered as a regular operation.

Objective To investigate the effects of NF-κB activation on paihogenesis of PMN aggregation in myocardium and early myocardial dysfunction induced by polymorphonuclear leukocyte (PMN) in burned rats. Method One hundred and seventy wistar rats were randomly divided into three groups, control group ( n = 20, with isotonic saline solution), bum group ( n = 90, with isotonic saline solution after bum), bum and pyrrolidine dithioncarbamate group ( PDTC group, n = 60, with isotonic saline solution and 250 mg/kg PDTC after bum). The rats in bum group and PDTC group underwent 35% TBSA full-thickness bums on the back. The acb'vaty of myocardial NF-κB was tested by electrophoretic mobility shift assay (EMSA) at 1, 3, 6, 12,24 postbum hours (PBH). Expressions of myocardial IL-8 and ICAM-1 mRNA were assessed by reverse transcription polymerase chain reaction (RT-PCR) at 3,6,12,24 PBH. Meanwhile, the avtivity of myocardial myeloperoxidse(MPO), the left ventricular systolic pressure(LVSP) and the left ventricular end diastolic pressure (LVEDP) as well as maximum positive and negative left ventricular pressure change (± dp/dtmax) were observed at 3,6,12,24 PBH. Results The activity of myocardial NF-κB in bum groups was markedly increased at 1 PBH [(20.27± 3.43) × 104 A] .which was obviously higher than that in control group [(2.18±0.38) × 104 A, P ＜ 0.01], and was still higher than that in control group at 24 PBH ( P ＜ 0.01). The expressions of myocardial IL-8 and ICAM-1 mRNA and avtivity of myocardial myeloperoxidse were obviously higher than that in control group at 3,6,12,24 PBH respectively (P ＜ 0.01), LVSP and ± dp/dtmax significantly were lower, but LVEDP was higher than that in control group during 3-24 PBH (P ＜ 0.01). Compared with that in bum group, these indices were ameliorated in PDTC group. Conclusions Severe bum might activate myocardial NF-κB, which ultimately lead to the production of cytokines, PMN aggregation in myocardium and deterioration of cardiac contractility and relaxation consequently.

Objective　To study the effects of measures for preventing early postburn damage in improving survival rate of burn patients during the third stage. Methods　12 568 burn cases admitted to our institute were chronically divided into three groups (1958-1980;1981-1990;1991-2000). Total burn surface area (TBSA), survival rate, incidence of burn shock, systemic infection and organ damage as well as the main treatments adopted in the recent decade were retrospectively analyzed. Results　Incidence of burn shock, systemic infection and organ damage were significantly lower, and the total survival rate and the survival rate in patients with different TBSA were markedly higher in the third group as compared with those in the first and the second group. Incidence of organ damage in patients treated with delayed fast fluid infusion, early escharectomy en masse, early enteral feeding, early prevention of inhalation injury and gut bacterial translocation were also significantly lower than in the control. Conclusion Measures taken in the third group for preventing early postburn damage play an important role in improving the survival rate of burn patients.

It is helpful to improve continuing education of TCM through learning American CME. The author described the strength and characteristic of American CME from the organization structure, the project organizer, the Physician's credit system and the trend of CME programs in the future. Discuss how to improve continuing education of TCM from changing the idea of sponsor, diversifying the ways of training and to build effect of evaluation mechanism.

AIM:To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS: The Angilent Zorbax,Extend C-18 column(4.6 mm×250 mm,5μm) was used with methanol-water (containing 0.4% phosphonic acid)(50: 50) as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS : The method had the good linear relationship within the range of 0.04-0.61 μg for luteolin and within the range of 0.20-3.01 μg for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence (264.8 μg/g,2 746.3 μg/g) in August,respectively.CONCLUSION: The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.

AIM:To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS:The Angilent Zorbax,Extend C-18 column(4.6 mm?250 mm,5 ?m)was used with methanol-water(containing 0.4% phosphonic acid)(50 ∶50)as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS:The method had the good linear relationship within the range of 0.04-0.61 ?g for luteolin and within the range of 0.20-3.01 ?g for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence(264.8 ?g/g,2 746.3 ?g/g)in August,respectively.CONCLUSION:The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.

AIM: To investigate the effects of burn sera on I?B? degradation, NF-?B activation in peripheral blood monocytes (PBMCs) in order to explore the role of burn sera on activation of monocytes. METHODS: PBMCs isolated from healthy volunteers were stimulated by sera from healthy volunteers and burn patients and by burn sera together with PDTC (pyrrolidine dithioncarbamate). Activation of monocytic NF-?B was tested by electrophoretic mobility shift assay (EMSA) and the degradation of monocytic I?B? was determined by Western blotting. RESULTS: When compared to that in control group, cytosolic I?B? degradation occurred within 30 min after PBMCs stimulated by burn sera, and peaked at 60 min. But I?B? gradually recovered in the cytoplasm after 2 h of stimulation. Meanwhile, activity of monocytic NF-?B was markedly increased, reached the peak at 30 min to 60 min after stimulation, and gradually decreased after 2 h of stimulation. PDTC (an antioxidants) effectively inhibited the monocytic I?B? degradation and activation of NF-?B induced by burn sera. CONCLUSION: Burn sera might induce the degradation of I?B?, then activate NF-?B, which ultimately lead to the secretion of cytokines from the monocytes. [