1.Effect of c-fos antisense gene transfection on protection of cardiomyocytes following hypoxia and burn serum treatment in rats
Journal of Third Military Medical University 2003;0(18):-
Objective To explore the effect of c fos antisense gene transfection on the protection of cardiomyocytes following hypoxia and burn serum treatment. Methods Burn serum was collected from Wistar rats with 30% total body surface area(TBSA) of Ⅲ degree burns. The mixture gas containing 1% O 2 was used as hypoxia model. The c fos antisense gene recombinant was constructed by genetic recombination technique. Cardiomyocytes from neonatal Wistar rats were cultured in vitro with hypoxia and burn serum treatment. c fos antisense gene recombinant was transfected into the cultured cardiomyocytes. Expression of c fos mRNA was determined by RT PCR. Expressions of c fos protein, troponin T and ? Tubulin in cardiomyocytes were determined by Western blotting in the transfected and non transfected groups. Results RT PCR results showed that the expression of c fos mRNA increased significantly in the non transfected group. But after transfection of c fos antisense gene recombinant, the expression of c fos mRNA decreased significantly as compared with the non transfected cardiomyocytes. Western blotting results showed that the expression of c fos protein in the transfected group decreased remarkably as compared with the non transfected group, but the expressions of ? Tubulin and troponin T increased significantly in the transfected group. Conclusion Burn serum and hypoxia can cause the injury of cardiomyocytes. c fos antisense gene recombinant transfection has the protective effect on cardiomyocytes exposed to burn serum and hypoxia.
2.Relation of c-jun antisense gene transfection and cardiomyocyte apoptosis following hypoxia and burn serum treatment
Journal of Third Military Medical University 2003;0(19):-
Objective To explore the relation of c jun antisense gene transfection and cardiomyocyte apoptosis following hypoxia and burn serum treatment Methods Burn serum was collected from Wistar rats with 30% total body surface area(TBSA) Ⅲ degree burn Rats inhaling mixed gas containing 1% O 2 was used as hypoxia model The c jun antisense gene recombinant was constructed by genetic recombination technique Cardiomyocytes from neonatal Wistar rats were cultured in vitro with hypoxia and burn serum treatment c jun antisense gene recombinant was transfected into the cultured cardiomyocytes Cardiomyocytes were stained with TUNEL for the examination of cardiomyocyte apoptosis at 12, 24 and 48 h after hypoxia and burn serum treatment In addition, the number of apoptotic cardiomyocytes was counted The results were processed statistically Results In the group with only the addition of burn serum and hypoxia(non transfected group), the numbers of apoptotic cardiomyocytes(mean per high power visual field) were 7 1?0 842, 28 4?1 635 and 13 2?1 525, respectively But after transfection of c jun antisense gene recombinant, numbers of apoptotic cardiomyocytes were 4 1?0 716,12 3?1 455 and 8 5?1 341, respectively There was a significant difference between the transfected group and the non transfected group( P
3.Molecular Mechanism of c-jun Antisense Gene Transfection in Protecting Cardiomyocyte from Injury of Hypoxia and Burn Serum Treatment
Journal of Chinese Physician 2000;0(12):-
Objective To explore the molecular mechanism of c-jun antisense gene transfection in protecting cardiomyocytes from injury of hypoxia and burn serum treatment. Methods Burn sera were collected from wistar rat with 30% total body surface area(TBSA) Ⅲ degree burn injury, and the mixture gas containning 1% O 2 was used as hypoxia model. The c-jun antisense gene recombinant vector was constructed. Neonatal wistar rat cardiomyocytes cultured in vitro were treated with hypoxia and burn sera. c-jun antisense gene recombinant vector was transfected into the cultured cardiomyocytes. Expressions of c-jun, PKC? and JNK were detected with western blot in the transfected and non-transfected cardiomyocytes. Results Expression levels of c-jun, PKC? and JNK significantly increased in the non-transfected cardiomyocytes when treated by hypoxia and burn sera, up to maximum 24 hour after the treatment. Expression levels of c-jun, PKC? and JNK in the transfected cardiomyocytes decreased significantly compared with non-transfected cells. Conclusions The transfection of the c-jun antisense gene recombinant vector protected cardiomyocytes from injury of hypoxia and burn sera treatment via down-regulating PKC? and JNK expressions.
4.Simultaneous determination of two compounds in Mosla chinensis by RP-HPLC
Haowu HU ; Xiaoming XIE ; Rengeng SHU ; Yuesheng WANG
Chinese Traditional Patent Medicine 2010;(1):88-91
AIM:To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS: The Angilent Zorbax,Extend C-18 column(4.6 mm×250 mm,5μm) was used with methanol-water (containing 0.4% phosphonic acid)(50: 50) as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS : The method had the good linear relationship within the range of 0.04-0.61 μg for luteolin and within the range of 0.20-3.01 μg for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence (264.8 μg/g,2 746.3 μg/g) in August,respectively.CONCLUSION: The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.
5.Simultaneous determination of two compounds in Mosla chinensis by RP-HPLC
Haowu HU ; Xiaoming XIE ; Rengeng SHU ; Yuesheng WANG
Chinese Traditional Patent Medicine 1992;0(01):-
AIM:To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS:The Angilent Zorbax,Extend C-18 column(4.6 mm?250 mm,5 ?m)was used with methanol-water(containing 0.4% phosphonic acid)(50 ∶50)as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS:The method had the good linear relationship within the range of 0.04-0.61 ?g for luteolin and within the range of 0.20-3.01 ?g for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence(264.8 ?g/g,2 746.3 ?g/g)in August,respectively.CONCLUSION:The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.
6.Study of flail chest surgery opportunity
Changhua DUAN ; Yuesheng HU ; Jingzhi LIANG ; Guangfia CHEN ; Jie LI ; Wei YANG
Chinese Journal of Primary Medicine and Pharmacy 2009;16(1):28-29
Objective To discuss the flail chest in the rib the fixed surgery opportunity,in fixed spot. Meth-ads Clinical data of 26 flail cheat patients in my courtyard were retrospectively analyzed. Results 26 patients were cured completely,nobody died,no obvious cheat abnormity was observed. Condusion Regarding the flail cheat pa-tient, at the right moment to adopt the fixed surgery in the rib, restore the integrity of the thoracic wall, may reduce the mortality rate, the chest shape normal, the quality of life obtains the enhancement.
7.Analysis of epidemiological characteristics of advanced schistosomiasis in Hu-nan Province,2012
Zhaochun LIU ; Shuiyuan XIAO ; Jie ZHOU ; Xinling YU ; Benjiao HU ; Jinhua ZHU ; Yuesheng LI
Chinese Journal of Schistosomiasis Control 2014;(2):148-152
Objective To understand the epidemiological characteristics of patients with advanced schistosomiasis in Hunan Province,so as to provide the evidence for formulating the advanced schistosomiasis prevention strategies and measures. Meth-ods The data of advanced schistosomiasis patients were collected and analyzed retrospectively with the cross section research method and description method in Hunan Province,2012. Results There were 5 722 advanced schistosomiasis patients in Hu-nan Province,and among them,4 112 patients were male(71.86%),and 1 610 were female(28.14%). Totally 5 311 patients came from the schistosomiasis endemic areas(92.82%)and 411 patients from non-schistosomiasis endemic areas(7.18%). The prevalence rate of advanced schistosomiasis was 8.46/10 000. The mean age of advanced schistosomiasis patients was 60.30 ± 11.63 years,and the youngest was 17 years old and the oldest 92 years old. In the age composition of advanced schistosomiasis pa-tients,the greatest number of cases was in the 60-70 years age group (32.72%). There were 3 595 cases of ascites type (62.83%),2107 cases of splenomegaly type(36.82%),11 cases of dwarf type(0.16%),and 11 cases of colon proliferation type (0.35%). Conclusion The prevalence rate of advanced schistosomiasis is relatively stable in Hunan Province,and the age of the patients showed an old aging trend. The salvation of advanced schistosomiasis patients in non-endemic areas should be strength-ened.
8. Effects of cardiac support on delayed resuscitation in extensively burned patients with shock
Rong XIAO ; Yuesheng HUANG ; Guoan LIN ; Shian YUAN ; Dongsheng HU
Chinese Journal of Burns 2018;34(1):8-13
Objective:
To explore the effects of cardiac support on delayed resuscitation in extensively burned patients with shock.
Methods:
Clinical data of 62 extensively burned patients with shock on admission, admitted to the 159th Hospital of PLA (hereinafter referred to as our hospital) from January 2012 to January 2017, were retrospectively analyzed. They were divided into cardiac support group (
9.Assessment report on infection control of schistosomiasis in China, 2008
Yang HAO ; Donghua YI ; Xianfeng ZHANG ; Jijie XIONG ; Wenzong YUAN ; Shoujing HU ; Xiaohua WU ; Rong ZHU ; Jiagang GUO ; Xibao HUANG ; Yuesheng LI ; Honggen CHEN ; Tianping WANG ; Xingqi DONG ; Huazhong LI ; Canjun ZHENG ; Zhao CHEN ; Liying WANG ; Xiaonong ZHOU
Chinese Journal of Schistosomiasis Control 2009;21(6):457-463
According to the requirement of the national assessment for achieving the infection control criteria, 42 villages (among them,25 villages belonged to the first stratum, and 17 villages belonged to the second stratum) in 14 counties from 5 provinces, including Hunnan, Hubei, Jiangxi, Anhui and Yunnan, were selected as sampling villages for the assessment.The results from the field assessment showed that 154 out of 9 067 people were found infected with Sckistosoma japonicum, with an average infection rate of 1.7% ranged from 0.31 % to 4.10% , and only Yongping Village from Weishan County and Tenglong Village from Eryuan County were not found any case. A total of 46 out of 3 323 head of cattle were infected with S. japonicum, with an average infection rate of 1.38% ranged from 0.26% to 3.79% , and no any infected individual detected in Nanling County. No outbreak occurred in those sampling villages. Therefore, it is indicated that the five sampling provinces have reached the national criteria on infection control of schistosomiasis.
10.Mutational profile of myeloproliferative neoplasms detected by a customized NGS-based gene panel
Xiu HUANG ; Xuan DENG ; Xiao XU ; Xinju ZHANG ; Yuesheng ZHANG ; Weizhe MA ; Tingting HU ; Gusheng TANG ; Ming GUAN
Chinese Journal of Laboratory Medicine 2019;42(6):427-434
Objective By a sequencing panel consisting of 50 targeted genes, aiming at depicting the molecular landscape of ET, PV, and PMF, which are three major subtypes of MPN, to provide valuable information in the diagnosis and prognosis of MPN.Methods A retrospective study was conducted of 53 patients from Huashan hospital and Changhai hospital. All patients were diagnosed in accordance with the 2016 WHO diagnostic criteria for MPN, including 31 cases of ET(11 males, 20 females, median age 55 years), 17 cases of PV(12 males, 5 females, median age 65 years), and 5 cases of PMF(4 males, 1 females, median age 67 years), and underwent next-generation of DNA sequencing of their bone marrow or blood samples. The genetic analyses were performed on bone marrow or peripheral blood. Referring to COSMIC, dbSNP, Clinvar and other public databases, we analyzed the sequencing data, and elucidated the mutation profile of MPN patients, combining with their clinic information. Results In addition to the typical JAK2, CALR, and MPL mutations, pathogenic mutations in other 11 genes were detected, as well as 4 SNPs that confer individual susceptibility to MPNs (rs4858647, rs9376092, rs58270997, rs621940). The average rate of mutated genes was 2.3 genes per patient. In all patients (53 cases), the mutated genes detected were TET2, EZH2, ASXL1, MIR662, SF3B1, BARD1, DNMT3A, KIT, RUNX1, TP53, NRAS according to their mutational frequency. Conclusions Applying next-generation sequencing technology, multi-gene sequencing of a bunch of typical BCR-ABL-negative MPN patients can be performed at one time within 2 working days, and pathogenic mutations other than JAK2, CALR, MPL can be found, which has a bright prospection in clinic.