AIM:To establish the normalized chromatographic fingerprints(NCFP) of Shegan Antiviral Injectionl(Rhizoma Belamcandae,Flos Lonicerae japonicae,Herba Artemisiae scopariae,Radix Bupleuri,etc.)(SGKBDI) and to evaluate them by using the 25 relatively normalized parameters.METHODS:The chromatographic fingerprints of the eight herbal drugs,two intermediates and preparation of SGI were determined by RP-HPLC.The 11 chromatographic fingerprints were evaluated by 25 relatively normalized index calculated by the software of Digitalized Evaluation System of Super-information Characteristic for Traditional Chinese Medicine Fingerprints 3.0.RESULTS:The best fingerprints were S4 Herba Artemislae Scopariae and S7 Radix Isatidis,the next were S2 Flos Lonicerae、 S5 Radix Bupleuri、 S6 Herba Taraxaci、 S8 Folium Isatidis,S10 intermediat Ⅱ and S11 Sgkbdi.CONCLUSION:The 25 characteristic parameters can be used to objectively,authentically and thoroughly reveal the separation of chromatographic fingerprints and its normalized characteristics of the NCFP of SGKBDI.

Objective: To investigate the expression of LC3B, p-AMPKα and p27 in cortical tuberous sclerosis complex (TSC). Methods: Nineteen specimens of surgically resected TSC cortical tubers were collected at Xuanwu Hospital, Capital Medical University, from 2014 to 2017. The expression of the three proteins in the lesions and the adjacent relatively normal regions was detected by immunohistochemical staining (EnVision two-step method). Results: LC3B was mainly expressed in the dysmorphic neuron and giant cell in TSC cortical tubers and in the adjacent relatively normal neurons, and the expression was diffuse or perinuclear cytoplasmic. There was no significant difference in the average optical density between abnormal cells and neurons adjacent to the lesions (0.343±0.195 vs. 0.419±0.088, P>0.05). p-AMPKα was localized in the cytoplasm of dysmorphic neurons and giant cell in TSC cortical tubers. The average optical density of abnormal cells in the lesions was significantly higher than that of neurons adjacent to the lesions (0.306±0.123 vs. 0.233±0.654, P<0.05). P27 showed nuclear positivity, mainly expressed in the neurons and glial cells close to TSC cortical tubers, while the positive rate in the abnormal cells in TSC cortical tubers was low (15/19 vs. 7/19, P<0.05). Conclusion There is no significant decrease in the level of autophagy in dysmorphic neurons and giant cells in TSC cortical tubers, which may be related to the compensatory mechanism of AMPK signaling pathway, but without activation of downstream p27.