1.Relationship of 5-hydroxy indole acetic acid, melatonin and dopamine in rat model of Parkinson's disease induced by 6-hydroxydopamine
Yuequn DU ; Tao MENG ; Zhihong ZHENG ; Ling LIN
Chinese Journal of Behavioral Medicine and Brain Science 2013;(4):293-295
Objective To investigate dynamic changes in extracellular striatal 5-hydroxy indole acetic acid (5-HIAA) and melatonin (MLT) concentrations in the 6-hydroxydopamine (6-OHDA)-induced Parkinson's disease (PD) rat models.Methods A hemiparkinsonium rat model was established by a unilateral injection of 6-OHDA into the right substantia nigra pars compacta (SNc) and the right medial forebrain bundle (MFB).Dialysate samples were collected at 10:00 in the lesioned striatum by microdialysis at 1,2,3 and 4 weeks post-lesion,respectively.The levels of dopamine (DA) and 5-HIAA,as well as MLT in dialysate sanples were measured by high performance liquid chromotorgraphy (HPLC).Results One week after surgery,the level of DA in PD group was (1.19±0.22) ng/ml,which was distinctly lower than those in the vehicle group ((8.62 ± 0.73) ng/ml) and the sham group ((8.73 ± 0.78) ng/ml) (P < 0.01).The levels of 5-HIAA in PD group had a downward trend,but there were no significant differences.The level of MLT in PD group was (43.31 ± 11.37) pg/ml at 4 weeks post-lesion,and significantly higher than those in the vehicle group ((19.13 ± 5.38)pg/ml) and the sham group ((19.80 ± 6.96) pg/ml) (P< 0.05).The striatal dialysate DA and 5-HIAA concentrations had a good positive correlation in the PD group (r =0.867,P < 0.01).Conclusion Decreased DA level concomitantly with changes of 5-HIAA and MLT in the ipsilateral striatum of 6-OHDA-induced PD rats,and these changes may be related to PD progression.
2.Efficacy and Safety of Insulin Glargine versus Premixed Insulin in Treatment of Type 2 Diabetes:A Me-ta-analysis
Kaijie CHEN ; Huifan YU ; Guangcui FANG ; Yuequn LIN ; Jinshan CHEN
China Pharmacy 2016;27(3):354-357
OBJECTIVE:To systematically review the efficacy and safety of insulin glargine versus premixed insulin in treat-ment of type 2 diabetes,and provide evidence-based reference for clinical treatment. METHODS:Retrieved from PubMed,CJFD, Wanfang Database and VIP Database,the randomized controlled trials(RCT)about the efficacy and safety of premixed insulin ver-sus insulin glargine in the treatment of type 2 diabetes were collected. Meta-analysis was performed by using Rev Man 5.0 statistics software after extracting data and evaluating quality by modified Jadad. RESULTS:A total of 10 RCTs were enrolled,involving 1 655 patients. Results of Meta-analysis showed that insulin glargine was better than premixed insulin on reducing glycated hemoglobin [M=-0.41,95% CI(-0.64,-0.18),P<0.001] and fasting blood glucose [MD=-0.51,95% CI(-0.99,-0.02),P=0.04], there were significantly differences between 2 groups,and there were no significantly differences in reducing 2 h postprandial blood glucose [MD=-0.56,95% CI(-1.21,0.09),P=0.09] and body mass index [MD=-0.52,95% CI(-1.52,0.48),P=0.31];the incidence of hypoglycemia in insulin glargine group was significantly lower than premixed insulin,there were significantly differ-ences between 2 groups [RR=0.65,95%CI(0.46,0.90),P=0.01]. CONCLUSIONS:The efficacy and safety of insulin glargine are better than premixed insulin in the treatment of type 2 diabetes.
3.Changes of melatonin and melatonin receptors on 6-OHDA induced Parkinson's disease in rat
Sutao YUAN ; Yuequn DU ; Tao MENG ; Ling LIN
Chinese Journal of Behavioral Medicine and Brain Science 2014;23(7):580-583
Objective To observe the changes of dopamine and its metabolites,melatonin,as well as melatonin receptors in the substantia nigras (SN) in 6-OHDA induced hemiparkinsonian rats.Methods PD rat was established by a unilateral injection of 6-OHDA into the right substantia nigra pars compacta and medial forebrain bundle.Rats were divided into PD groups and vehicle groups,and sacrificed at 10:00 and 22:00 respectively,at 3 and 6 weeks post-lesion.The contents of dopamine and its metabolites as well as melatonin were measured by HPLC.The expressions of MT1 and MT2 mRNA and protein were detected by RT-PCR and Western-Blot respectively.Results Dopamine levels declined by 83% and 88% respectively at 3 and 6 weeks post-lesion in the injected side of PD rats,compared with the contralateral side.Melatonin levels in the injected side increased by 22% and 29% respectively.However,melatonin increased by 46% and 31% respectively at 10:00 and 22:00,at 6 weeks post-lesion in the contralateral side,compared with the same side of the vehicle.The expressions of MT1 and MT2 receptors mRNA were significantly decreased by 91.04% and 90.24% respectively in comparison with the vehicle,while the protein expression of MT1 and MT2 receptors significantly declined by 58% and 70% respectively.Conclusion Increased melatonin and down-regulated MT1 and MT2 receptors in the lesioned SN in 6-OHDA-induced rats may be associated with the progression of PD.