Objective To observe and evaluate the effect of glycolic acid peels in the treatment of acne and scars,and to approach the indications,process and security.Methods 34 patients with acne were chosen.Glycolic acid of 20 ％,35 ％,50 ％,and 70 ％ concentration was used for chemical peeling therapy for 5 times.The concentration of glycolic acid was increased every three weeks.The efficacy was evaluated by comparison of the lesion counts before and after the course.Patients were assigned to fulfil the questionnaire to assess the follicular orifice,secretion of sebaceous glands,smooths,whiteness,scar,influence to living and satisfaction.Results 34 patients completed the course.4 patients were cured,15 patients were greatly improved,12 patients were improved,and 3 patients had no effectiveness.The efficacy rate was 91.18 ％.After treatment,73.53 ％ of the patients showed improvement in follicular orifice,55.88 ％ of the patients showed that secretion of sebaceous glands was decreased,82.35 ％ of the patients had improvement in smoothness,61.77 ％ of the patients had improvement in whiteness,and 67.65 ％ of the patients had decrease in scar formation.Conclusions Glycolic acid peeling is proved an effective,well accepted,and safe treatment for mild to moderate acne.The follicular orifice,secretion of sebaceous glands,skin smoothness,whiteness and scars could be also improved after treatment.

Objective To explore the modified mesh in female pelvic floor reconstruction for pelvic organ prolapse and clinical effects of the application.Methods 78 patients with pelvic organ prolapse,line modified mesh pelvic floor reconstruction surgery(study group)and vaginal hysterectomy and vaginal repair of anterior and posterior wall(control group)treatment,clinical efficacy was compared.Results The mean operating time,blood loss,average body temperature,catheter indwelling time,residual urine volume,and postoperative hospital stay showed no significant difference(P＞0.05).No postoperative infection,vaginal hematoma,mesh erosion occurred.Two groups of patients before discharge degree of POP-Q points are 0°or Ⅰ degree,to cure the standard.Vaginal length of the control group was significantly shorter than after treatment(P＜0.05),while no significant change in the observation group(P＞ 0.05).Control group,the recurrence rate was significantly higher than the observation group(P＜0.05),while the observation group of sexual satisfaction was better than the control group(P＜0.05).Conclusion The modified mesh treatment of female pelvic floor reconstruction is an effective method of pelvic organ prolapse,the surgical curative effect is better,the recurrence rate is lower,worthy of clinical application.

AIM: To evaluate the efficacy and the safety of betamethasone neomycin ointment in treatment of patients with subacute eczema. METHODS: The clinical trial was performed on patients with limited, subacute eczema by a randomized double blind method. Hydrocortisone neomycin ointment was chosen as controls. RESULTS: There were 45 patients involved in this study, including 23 patients in betamethasone neomycin ointment group and 22 patients in hydrocortisone neomycin ointment group. The total integra decrease of betamethasone neomycin ointment group [ 12.26 ? 2.42 to 5.61 ? 2.23 ( d 7 ), 2.83 ? 2.06 ( d 14 )] was better than that of hydrocortisone neomycin ointment group [ 13.18 ? 2.28 to 9.18 ? 3.72 ( d 7 ), 6.82 ? 3.46 ( d 14 )] on the 7th and 14th day after the treatment (P

Objective To explore the role of mouse dermis-derived mesenchymal stem cells (mdMSC) on skin repair. Methods mdMSC and human dermal fibroblasts were isolated and identified. Human dermal fibroblasts were cultured alone or eoeultured with mdMSC in Transwell chambers with the density ratio of human dermal fibroblasts to mdMSC being 2/5, 1/1, and 2/1. On day 4 and 8 of culture, the expression levels of hydroxyproline and transforming growth factor-beta (TGF-beta) 1 were measured in the supematant of monoculture and coculture by alkaline hydrolysis and ELISA respectively. Results The level of hydro-xyproline was significantly higher in the supematants of coculture system with a density ratio of 2/5 and 1/1 than that in monoculture supematants of human dermal fibroblasts on day 8 (both P < 0.05). Elevated level of TGF-betal was observed in all coculture supematants on day 8 (all P < 0.01) and in the supernatants of coculture system with a density ratio of 1/1 on day 4 (P < 0.05). There was no significant correlation between the expression level of TGF-betal and hydroxyproline in the coculture supernatants (r = 0.108, P ＞ 0.05). Conclusion In vitro coculture with mdMSC can increase the production of hydroxyproline and TGF-betal by fibroblasts, which may be a mechanism underlying the facilitation of skin repair by mdMSC.

Objectives To detect anti-basement membrane zone(BMZ)antibodies in systemic lupus ery-thematosus(SLE)patients and to explore its association with clinical manifestations.Methods Anti-BMZ anti-bodies were examined by indirect immunofluorescence in the sera of70patients with SLE.The correlation between anti-BMZ antibodies and clinical data of SLE was analyzed.Results Anti-BMZ antibodies could be found in the sera of about70%SLE patients,including IgG,IgM,IgA.They predominantly bound to the epidermis,but also bound to the dermis or both.The positive rate of anti-BMZ antibodies was significantly higher in patients with skin lesions than that of patients without skin lesions.There is no significant difference between the two groups in ac-tive and remission,kidney involvement,arthritis,alopecia,photoallergy,positive anti-dsDNA antibodies and the posi-tive rate of anti-BMZ antibodies.Conclusion Anti-BMZ antibodies presents in the sera of SLE patients with high positive rate.It is correlated with the development of skin lesions of SLE patients,but not with the activity of SLE,other clinical manifestations and anti-dsDNA antibodies.Anti-BMZ antibodies may be involved in the pathogenlic mechanism of the development of skin lesions in SLE patients.

Background and Objectives: The application of adipose derived stem cells (ADSCs) in skin repair has attracted much attention nowadays. Epidermal growth factor (EGF) participates in the progress of skin proliferation, differentiation and so forth. We aimed to explore the role of EGF in the proliferation, invasion, migration and transdifferentiation into epidermal cell phenotypes of ADSCs. Methods: and Results: ADSCs were extracted from adipose tissues from patient. Immunophenotyping was determined by flow cytometry. Overexpressed EGF or siEGF was transfected by lentiviruses. EGF was determined by enzyme linked immunosorbent assay (ELISA) or western blot. ADSCs and HaCaT cells were co-cultured by Transwell chambers. Conditioned medium (CM) was obtained from cultured HaCaT cells and used for the culturing of ADSCs. Cell viability was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Invasion rate was measured by Transwell invasion assay and migration rate by wound healing test. mRNA and protein levels were measured by qPCR and western blot respectively. The extracted cells from adipose tissues were identified as ADSCs by morphology and immunophenotyping. The expression of EGF was up or down regulated constantly in HaCaT cell line after transfection. EGF overexpression upregulated the proliferation, migration and invasion rates of ADSCs, and EGF expression regulated the expression of cytokeratin-19 (CK19) and integrin-β as well. Conclusions EGF could be served as a stimulus to promote the proliferation, migration, and invasion as well as the transdifferentiation into epidermal stem cell immunophenotyping of ADSCs. The results showed that EGF had a promising effect on the repair of skin wound.

Objective To examine the dose-response relationship between gestational weight gain rate and the neonate birth weight.Methods A total of 18 868 women with singleton gestations who delivered between January 2006 and December 2013 were included in this study.Maternal and neonate details of these women were drawn from the Perinatal Monitoring System database.Gestational weight gain rate was defined as the total weight gain during the last and first prenatal care visits divided by the interval weeks.Both Multiple logistic regression analysis and restricted cubic spline methods were performed.Confounding factors included maternal age,education,pre-pregnancy body mass index (BMI),state of residence,parity,gestational weeks of prenatal care entry,and sex of the neonate.Results The adjusted odds ratio for macrosomia was associated with gestational weight gain rate in lower pre-pregnancy BMI (OR=3.15,95％CI:1.40-7.07),normal (OR=3.64,95％CI:2.84-4.66) or overweight (OR=2.37,95％CI:1.71-3.27).The odds ratios of low birth weight appeared a decrease in those women with lower pre-pregnancy BMI (OR=0.28,95％CI:0.13-0.61) while the normal weight (OR=0.37,95％CI:0.22-0.64) group with gestational weight gain,the rate showed an increase.Association of gestational weight gain rate for macrosomia was found a S-curve in those term delivery women (non-linearity test P＜0.000 1).However,L-curve was observed for low birth weight and gestational weight gain rate in term births (non-linearity test P＜0.000 1).Conclusion A S-curve was seen between gestational weight gain rate and term delivered macrosomia while L-curve was observed among term delivered low birth weight neonates.