1.Radiological diagnosis and best choose of pituitary adenomas
Dongkui YANG ; Lei ZHENG ; Yuelong SUN ; Zhijun LI
Chinese Medical Equipment Journal 1989;0(01):-
10mm in diameter) which were mostly non-functional ones(63.3%), and 12 microadenomas(≤10mm in diameter) which were mostly functional ones(91.7%). Only macroadenonas had abnormal imaging in DR, DSA, while microadenomas had no change in above radiological examinations. Microadenomas appeared in low density on CT or hypointensity in MRI as the direct signs, and CT, MRI contrast enhancement raised its display rate greatly. Macroadenomas showed various contrast in the post enhancement CT or MRI. Contrast enhancement of CT or MRI were capable of delineating size, location, extent of the tumor and the remaining intact pituitary tissue. Conclusion MRI Gd-DTPA contrast enhancement scaning is the first-selecting method to diagnose the pituitary adenomas especially the clinical suspected cases.
2. Characterization and analysis of VH1-2-encoded heterosubtypic antibodies isolated from an avian H5N1 patient.
Ying SUN ; Tian BAI ; Zi LI ; Feier RUAN ; Lingling CHEN ; Jian LU ; Liqi LIU ; Dayan WANG ; Yuelong SHU ; Kun QIN ; Jianfang ZHOU
Chinese Journal of Experimental and Clinical Virology 2018;32(2):136-139
Objective:
To isolate the cross-reactive antibodies against hemagglutinin of influenza virus and identify its biological function.
Methods:
The antibodies gene reservoir of cross-reactive and H5N1 pseudotype particles neutralizing B cell circulating in peripheral blood of a human H5N1 case was recovered by
3. Generation and preliminary characterization of monoclonal antibodies against neuraminidase of H7N9 subtype influenza A virus
Yueyang YU ; Yiran XIE ; Yingzhu CHEN ; Ying SUN ; Heng ZHANG ; Jian LU ; Liqi LIU ; Dayan WANG ; Yuelong SHU ; Kun QIN ; Jianfang ZHOU
Chinese Journal of Experimental and Clinical Virology 2017;31(1):66-70
Objective:
To develop the monoclonal antibody (mAb) against neuraminidase of H7N9 subtype influenza A virus and identify its biological function.
Methods:
Female 8 week-old BALB/c mice were immunized and the splenocytes of the mice were fused with Sp2/0 myeloma cells. Indirect ELISA was used to screen hybridoma and the positive clones were subject to be subcloned. Positive clones were identified and the monoclonal antibodies(mAbs) were obtained by purifying the ascetic fluid of mice injected with the hybridoma. The NA-binding as well as neuraminidase-inhibition activity of these mAbs were determined.
Results:
Three mAbs against neuraminidase of H7N9 subtype influenza A virus, 1G8, 3C4 and 4E8, were obtained. They demonstrated different epitop-recognizing. 3C4 and 4E8 exhibited neuraminidase inhibitory activity, with a IC50 of 1.45 μg/ml and 8.65 μg/ml, respectively.
Conclusions
The results suggested that mAbs specific to neuraminidase of H7N9 subtype influenza A virus were developed, providing an useful tool in control and preventing the novel H7N9 influenza A virus.
4.Working conditions of bipolar radiofrequency on human articular cartilage repair following thermal injury during arthroscopy.
Yuelong HUANG ; Yujun ZHANG ; Xiaoquan DING ; Songyang LIU ; Tiezheng SUN
Chinese Medical Journal 2014;127(22):3881-3886
BACKGROUNDThe thermal injury during bipolar radiofrequercy results in chondrocyte death that limits cartilage repair. The purpose was to determine the effects of various factors of bipolar radiofrequency on human articular cartilage after thermal injury, offering suitable working conditions for bipolar radiofrequency during arthroscopy.
METHODSOsteochondral explants from 28 patients undergoing total knee arthroplasty (TKA) in Department of Orthopaedic, Peking University Reople's Hospital from October 2013 to May 2014, were harvested and treated using bipolar radiofrequency in a light contact mode under the following conditions: various power setting of levels 2, 4 and 6; different durations of 2 seconds, 5 seconds and 10 seconds; irrigation with fluids of different temperatures of 4°C, 22°C, and 37°C; two different bipolar radiofrequency probes ArthroCare TriStar 50 and Paragon T2. The percentage of cell death and depth of cell death were quantified with laser confocal microscopy. The content of proteoglycan elution at different temperatures was determined by spectrophotometer at 530 nm.
RESULTSChondrocyte mortality during the treatment time of 2 seconds and power setting of level 2 was significantly lower than that with long duration or in higher level groups (time: P = 0.001; power: P = 0.001). The percentage of cell death after thermal injury was gradually reduced by increasing the temperature of the irrigation solutions (P = 0.003), the depth of dead chondrocytes in the 37°C solution group was significantly less than those in the 4°C and 22°C groups (P = 0.001). The proteoglycan elution was also gradually reduced by increasing the temperature (P = 0.004). Compared with the ArthroCare TriStar 50 group, the percentage of cell death in the Paragon T2 group was significantly decreased (P = 0.046).
CONCLUSIONSThermal chondroplasty with bipolar radiofrequency resulted in defined margins of chondrocyte death under controlled conditions. The least cartilage damage during thermal chondroplasty could be achieved with lower power, shorter duration, suitable temperature of irrigation solutions and chondroprotective probes. The recommendations for the use of bipolar radiofrequency to minimize cartilage damage could be achieved with a power setting of level 2, treatment duration of 2 seconds, suitable fluid temperature (closer to body temperature of 37°C) and chondroprotective Paragon T2 probes.
Arthroplasty, Replacement, Knee ; methods ; Cartilage, Articular ; surgery ; Catheter Ablation ; methods ; Cell Survival ; physiology ; Chondrocytes ; pathology ; Humans ; Microscopy, Confocal
5.A logistic regression model for prediction of glioma grading based on radiomics.
Xianting SUN ; Weihua LIAO ; Dong CAO ; Yuelong ZHAO ; Gaofeng ZHOU ; Dongcui WANG ; Yitao MAO
Journal of Central South University(Medical Sciences) 2021;46(4):385-392
OBJECTIVES:
Glioma is the most common intracranial primary tumor in central nervous system. Glioma grading possesses important guiding significance for the selection of clinical treatment and follow-up plan, and the assessment of prognosis. This study aims to explore the feasibility of logistic regression model based on radiomics to predict glioma grading.
METHODS:
Retrospective analysis was performed on 146 glioma patients with confirmed pathological diagnosis from January, 2012 to December, 2018. A total of 41 radiomics features were extracted from contrast-enhanced T
RESULTS:
A total of 5 imaging features selected by LASSO were used to establish a logistic regression model for predicting glioma grading. The model showed good discrimination with AUC value of 0.919. Hosmer-Lemeshow test showed no significant difference between the calibration curve and the ideal curve (
CONCLUSIONS
The logistic regression model using radiomics exhibits a relatively high accuracy for predicting glioma grading, which may serve as a complementary tool for preoperative prediction of giloma grading.
Brain Neoplasms/diagnostic imaging*
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Glioma/diagnostic imaging*
;
Humans
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Logistic Models
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Magnetic Resonance Imaging
;
ROC Curve
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Retrospective Studies
6. Advances in basic and clinical research on hepatocellular carcinoma in 2016
Zhizhen DONG ; Xiaodong ZHU ; Zhao LI ; Yuge MAO ; Yuelong CHAI ; Dengfu YAO ; Huichuan SUN ; Jiye ZHU ; Kuansheng MA
Chinese Journal of Hepatology 2017;25(2):85-93
Hepatocellular carcinoma (HCC) is still one of common malignant cancers worldwide, with increasing incidence and mortality rates. Early diagnosis and effective treatment for HCC remain to be explored. This article introduces the research advances in the early specific diagnosis and effective therapies for HCC in 2016, such as molecular markers in the specific diagnosis and targeted therapy for HCC, main therapeutic regimens, robot-assisted liver resection, and no-touch radiofrequency ablation.