Objective: To study the color reproduction ratio of metal ceramic restoration in clinic. Methods:The color of 150 maxillary incisors and its metal ceramic restorations was measured by MinoltCR 321 chromaticity instrument(D65 daylight simulator inside),the color reproduction ratio was calculated with CIE 1976 L*a*b* system. Results: The color reproduction ratio of metal ceramic restoration measured by clinical eye evaluation was 33.9%.Color error ratio was 66.1%. Conclusion: The color reproduction ratio measured by eye evaluation is low and the color error ratio is high.

Objective: To obtain the data of the surfaces of stand ar d posterior teeth crowns with an improved measurement system, so as to lay the f oundation for fabrication of dental prosthesis with computer aided design and la ser solid forming technology. Methods: Standard posterior teeth models were measured with a numerical controlled miller and a new data col lection system by cutting and scanning layer by layer, and the 3 dimension (3 D) shape of the models was reconstructed by image processing with a special soft ware. Results: Data of image profile and 3D shape of sixte en posterior teeth crowns were obtained. Precise configuration was acquired.Conclusion: Data of image profile can be easily and clearly ob tained by the improved measurement system with no measuring blind spot.

The prognosis of attention deficit hyperactivity disorder(ADHD)is poor,and approximately two third patients still have obvious symptoms when they grow up into adults,thus interfering with their social functions including work,study,family and interpersonal relation etc,and even causing the mental disorders and/or antisocial personality disorder.The predictive factors of adult outcome of ADHD include genetic,environmental and clinical factors.This review deals with the adult outcome and its predictive factors,and explores the severes harmfulness against individual,family and society brought by ADHD and its underlying mechanisms so as to facilitate the clinical intervention and research.

Objective To study the loss of imprinting(LOI) of insulin-like growth factor Ⅱ(IGF2) and H19 genes in cervical carcinomas.Methods Polymerase chain reaction(PCR) method was used on DNA samples extracted from 40 cases of cervical carcinoma and 20 cases of normal control tissues to detect the heterozygosity of IGF2 and H19 genes.The LOI of IGF2 and H19 genes was detected by RT-PCR.Results The heterozygous frequency of IGF2 was 52% in cervical cancer samples.Comparatively,in 20 matched normal samples,13 cases showed the heterozygosity(65%).The biallelic expression of IGF2 was detected in 10 cases among the 21 informative cervical cancer samples(47.6%),however,1 case in 13 heterozygous samples of control group(7.7%)((P0.05).Conclusion LOI and LOH of IGF-2 and H19 genes are involved in cervical carcinomas.LOI of IGF2 and H19 may be involved in the initiation stage of carcinogenesis of cervical carcinoma.

Objective: To reconstruct 3D shape of upper edentulous jaw f or the design of a digital model of palatal metal plate of upper complete dentur e. Methods: Standard edentulous jaw models were measured with a numerical controlled miller and a new data collection system by cutting and sca nning layer-by-layer, the 3D shape of the models was reconstructed by image pr ocessing with special software. Based on these data, a palatal metal plate of u pper complete denture was designed by using SURFACER softerware.Results: Data of upper edentulous jaw models and a palatal metal plate of upper c omplete denture were obtained.The error between basal surface of the plate base and uper endentulous jaw was 0.0245 ?0.0267 mm. Conclusion:E dentulous jaw can be reconstructed by three-dimensional measurement, the design ed palatal metal plate of upper complete denture will be helpful to the further Laser Solid Forming.

Objective:To investigate the microstructure and mechanical property of titanium parts made by laser solid forming technology. Methods: Titanium parts were made by laser solid forming technology; microstructure and elements were observed by SEM and spectrum analysis. The mechanical property of laser formed titanium and TA2 titanium were measured by Omnipotent testing machine. Results: The microstructure of titanium parts fabricated by laser rapid forming has columnar crystals with regular and epitaxial growth. The inner structure is dense and acicular structure distributed uniform at all field with width about 3-4 ?m; No obvious impurity elements were observed; the tensile-strength and yield strength of titanium parts is obviously superior to the standard of TA2. Conclusion: The microstructure of titanium parts made by laser solid forming can be obviously improved and mechanics performance was greatly improved. Those titanium parts may be used for dental restoration.

Objective To investigate the expressions of estrogen receptor (ER) subtypes and c-met proto-oncogene in human endometrial carcinomas and to assess the clinical significance of ER and c-met in this carcinoma. Methods Reverse transcription PCR (RT-PCR) was used to detect the expressions of ERα, ERβ and c-met proto-oncogene mRNA in 30 samples of endometrial carcinoma and 11 samples of normal endometrium. Results The expression of ERα in endometrial carcinoma (0.70±0.40) was significantly reduced in comparison to that in normal endometrium (1.14±0.56, P<0.05). A similar finding was made for the expression of ERβ in carcinoma (0.24±0.18) versus normal tissues (0.48±0.20, P<0.05). In contrast, c-met mRNA expression was increased in endometrial carcinoma (1.45±0.72) compared to that in normal endometrium (0.42±0.31, P<0.01). A decrease tendency of the expression of ERα was also found from Stage Ⅰ (0.82±0.41) to a more severe Stag Ⅱ-Ⅲ of endometrial carcinoma (0.42±0.17, P<0.05). The analysis of ERα and ERβ mRNA revealed a decrease tendency from shallow to deep invasion of the uterine muscles (P<0.05). We found that the expressions of ERα and ERβ were negatively correlated with c-met proto-oncogene with a coefficient correlation of -0.63 (P<0.01) and -0.32 (P<0.05), respectively. Conclusion ERα and ERβ are both involved in mutagenic action of carcinogen. C-met proto-oncogene plays an important role in the carcinogenesis and development of endometrial carcinoma. C-met and ER expressions show a negative correlation in the development of endometrial carcinoma.

Objective:To investigate the association between selenoprotein P (Sepp1) and Brucella infection at gene and protein levels. Methods:In this case-control study, 32 patients with chronic brucellosis (referred to as brucellosis) in Jiuyuan District and Bayan Obo Mining District of Baotou City from June to September 2019 were selected as brucellosis group, and 30 healthy people with the same epidemiological environment as the brucellosis group were selected as control group. The two groups were Han nationality. The single nucleotide polymorphism (SNP) of Sepp1 gene(rs7579) in 32 brucellosis patients and 30 healthy controls were tested by amplification refractory mutation system-PCR (ARMS-PCR). And the Sepp1 protein content was measured by enzyme linked immunosorbent assay (ELISA).Results:Thirty-two cases of brucellosis group, including 6 females and 26 males, aged (50.312 ± 5.035) years; 30 cases of control group, including 4 females and 26 males, aged (49.994 ± 5.098) years. There were no significant differences in sex ratio and age between the two groups (χ 2=0.336, t = 1.744, P > 0.05). There were no significant differences in the frequency distribution of G, A allele and GG, GA, and AA genotypes of rs7579 locus of Sepp1 gene between brucellosis group and control group (χ 2=0.263, 0.942, P > 0.05). In stratified analysis, there was a statistically significant difference in the frequency distribution of genotypes between female brucellosis group and control group (GG: 0/6, GA: 2/6, AA: 4/6; GG: 4/4, GA: 0/4, AA: 0/4, P < 0.05). And the level of Sepp1 protein in brucellosis group was significantly lower than that in control group (13.71±0.32, 19.26±0.69, t = 20.316, P < 0.05), low level of Sepp1 was a risk factor for brucellosis ( OR = 1.512, 95% CI: 1.290 - 1.687). Conclusions:The SNP of Sepp1 gene (rs7579) plays a role in Brucella infection, G allele may be the protective factor for women. The low level of Sepp1 protein is also associated with brucellosis.

Objective To explore the clinical effect of holistic nursing in the operation room for surgical children patients. Methods 120 patients required surgical treatment were selected and divided into the control group(45 cases)and the observation group(75 cases)randomly.The control group was given routine nursing,the observation group was given holistic nursing including preoperative interview,psychological care and all the preparations,strict check upon going into the operation room and before surgery,to help children keep graceful position,to cooperate closely with the anesthetist and surgeon,to observe the condition of patients and give good infusion management,to give continued observation of the disease condition after surgery,to make children safe escort back to the ward,and to make transfer with ward nurses.The treatment effect was compared between two groups. Results Two groups of children passed the period of operation and anesthesia safely,without appearing accidental situation.The satisfaction degree of the observation group was significantly higher than the control group.One case appeared operation accident and two cases appeared anesthesia awareness in the control group,and they were correctly handled.No one appeared operation accident and anesthesia awareness in the observation group.The differences between the two groups were significant. Conclusions Application of holistic nursing in the operation room for children patients puts forward higher requirements for nurses.It can help children cooperate with surgery better to achieve better surgical results,which is worthy of promotion.

Objective To investigate the effect of intrathecal administration of a mixture of butorphanol and ketamine on cAMP-PKA-CREB signal transductian pathway in the spinal dorsal ham of the rats with inflammatory pain. Methods Twenty-four male SD rats, weighing 240-280 g,in which intrathecal catheters were successfully placed, were divided into 4 groups randomly (n = 6 each): inflammatory pain group (group IP), butorphanol group (group B), ketamine group (group K), and butorphanol + ketamine group (group BK). The inflammatory pain was induced by injection of 5% formalin 50 μl into the plantar surface of left hind paw. Normal saline 10 μl, butorphannl 12.5 μg, ketamine 50 μg, and a mixture of butorphanol 12.5 μg and ketamine 50 μg was injected intrathecally 30 min before subcutaneous injection of formalin in group IP, B, K and BK respectively.Pain intensity score (PIS) was used to assess pain behavior every 5 min within an hour after subcutaneous injection of formalin. The animals were killed at 2 h after subcutaneous injection of formalin, and the L5 segment of the spinal cord was removed for determination of protein kinase A (PKA) and phosphorylated cAMP response element binding protein (p-CREB) expression using immunohistochemistry. Results Fonnahn administration induced pain behaviour expressed as two phases. PIS scores, PKA and p-CBEB expression, and staining scores were significantly lower during the fast and second phases in group BK than in group IP (P < 0.05 or 0.01), while no significant differences were found in the indices mentioned above between group B and IP and between group K and IP (P>0.05). Conclusion lntrathecal injection of a mixture of butorphanol and ketamine can reduce inflammatory pain in rats, and the mechanism may be related to the cAMP-PKA-CREB signal transduction pathway.