This study was aimed to establish an identification method between leaflets of Dimocarpus longan and leaflets of Litchi chinensis. The leaflet morphological-venation pattern (LMVP) and quantitative analysis were reliable identification method for Chinese herbs. The results showed that the main differences of leaflets of Dimocarpus lon-gan were the eucamptodromous camptodromous pinnate venation; the secondary veins gradually changed into little near margin and a few brochidodromous; the type of tertiary vein was percurrent or reticulate. The main differences of leaflets of Litchi chinensis were brochidodromous camptodromous pinnate venation; the course of the secondary veins was sinuous or zigzag, and abruptly curved then linked; the type of tertiary vein was reticulate. With three groups of key differences mentioned above, both plants can be successfully identified from each other. The accuracy of identification results (AC) was from 98.1% to 100%. The agreement rate for observation (ARO) was from 98.5% to 100%. And the Kappa value was from 0.97 to 1.00. It was concluded that the established LMVP is simple, rapid, e-conomic and reliable in the identification between leaflets of Dimoc arp us longan and leaflets of its confused herb Litchi chinensis.

Objective To study the relationship between the number of outpatient complaints and the time or the outpatient amounts.Methods A retrospective analysis of 1289 cases of outpatients' medical complaints with basic records since 2009 in our hospital was carried out.The time regularity of the number of outpatient complaints,or the complaint rate was analyzed.And the correlation between the outpatient amounts and the number of outpatient complaints,or the complaint rate was also analyzed.Results In our hospital,the mean monthly number of outpatient complaints was (23.44±8.41)cases; the mean monthly outpatient account was (246 000±27 417)cases.The differences among monthly outpatient complaints,outpatient accounts and monthly complaints rate among every years were statistically significant.Within one year,the outpatient complaints numbers were lowest in February and January.And the outpatient complaints rate was low in November,January,February and December.There were statistically significant correlation between the monthly outpatient complaints and monthly outpatient accounts,and between the monthly outpatient complaints rate and monthly outpatient accounts.Conclusions There was time regularity in the outpatient complaints rates in one year.And there was certain correlation between the outpatient complaints rate and the outpatients' accounts.

OBJECTIVETo study the leaf venation characteristics of Bidens pilosa and B. pilosa var. radiata and establish an identification method.

METHODLMVP (leaf morphological-venation pattern for identification Chinese herbs), and QAERM (quantitatively analyze and evaluate reliability for the method of identification Chinese herbs) were applied for the study.

RESULTUnder the transmission-light, the tertiary vein of B. pilosa was discrete, the color was darker, the size was bigger, the shape was short curve, short linear, spot-like and branch-like. However the tertiary vein of B. pilosa var. radiata was continuous linear and color lighter. With the mentioned key difference, the both plants could be successfully identified from each other, the accuracy of identification results (AC) was from 96.7% to 97.7%. The repeatability of identification results: agreement rate for observation (ARO) was 95.1% and Kappa value was 0.90.

CONCLUSIONThe established method is simple, rapid, economic and reliable.

Bidens ; anatomy & histology ; chemistry ; Pigmentation ; Plant Leaves ; anatomy & histology ; chemistry

OBJECTIVETo observe and study the correctness of the proposition dicot have free vein termination but monocot have not. To evaluate the reliability of the identification principle that is based on above proposition.

METHODFourteen species of pharmaceutical monocot come from 9 families and 3 species of pharmaceutical dicot come from 3 families were observed by using the method of microscopic identification.

RESULTThe free vein termination existed in the vein-islets of 10 species of monocot from 8 families, Zingiberaceae, Stemonaceae, Gramineae, Liliaceae, Orchidaceae, Alismataceae, Dioscoreaceae and Araceae ect, and it was not to find the free vein termination in monocot Iris tectorum, Belamcanda chinensis (Fam. Iridaceae) and Imperata cylindrica, Coix lacryma-jobi (Fam. Gramineae). Three species dicot, Cinnamomum burmannii, Castanea mollissima, Mangifera indica, all had the free vein termination. The free vein termination of C. burnmannii was sparse, and C. mollissima's was usually covered by non-glandular hair.

CONCLUSIONThe proposition is not universally applicable, monocot have no free vein termination. The identification principle is unreliable, which distinguish between medicinal dicot and monocot leaves according to whether the free vein termination exists or not.

Magnoliopsida ; anatomy & histology ; classification ; Plant Leaves ; anatomy & histology ; Plants, Medicinal ; anatomy & histology ; classification ; Reproducibility of Results

OBJECTIVETo study the identification characters of Abutilon indicum and its confused herb A. theophrasti and establish an identification method.

METHODLMVP (leaf morphological-venation pattern for identification Chinese herbs), and QAERM (quantitatively analyze and evaluate reliability for the method of identification Chinese herbs) were applied for the study.

RESULTThe main differences of the leaf of Abutilon indicum: leaf margin cilia short and not clear (75-200 microm), petiole top with long straight hairs (up to 1.0-1.2 cm), when the tertiary veins and intersecondary veins issue from the lower part of midrib, their angles of divergence usually were wide acute (65 degrees-80 degrees) and a few was about right angle (80 degrees-100 degrees). The main differences of the leaf of A. theophrasti: leaf margin cilia long and clear (200-500 microm), petiole top with short straight hairs (most 0.1-0.5 mm), when the tertiary veins and intersecondary veins issue from the lower part of midrib, their angles of divergence usually were about right angle (80 degrees-100 degrees) and a few was wide acute (65 degrees-80 degrees). With the mentioned three groups of key differences, the both plants could be successfully identified from each other. The accuracy of identification results (AC) was from 97.5% to 99.6%, the repeatability of identification results: agreement rate for observation (ARO) was 97.1% and Kappa value was 0.94.

CONCLUSIONThe established method is simple, rapid, economic and reliable.

Malvaceae ; anatomy & histology ; classification ; growth & development ; Plant Leaves ; anatomy & histology ; classification ; growth & development

OBJECTIVETo assess the relationship between microglia activation and apoptosis of neurons, and the significance of activated microglias in the formation and progression of senile plaques in Alzheimer's disease.

METHODSIL-1alpha and beta-amyloid immunohistochemistry, combined with TUNEL assay were used to assess brain tissue samples from 10 patients with Alzheimer's disease and 4 negative control cases without neurological disease.

RESULTSThe number of resting microglias in the brains of Alzheimer's disease patients was similar to that of the control group (P > 0.05), but the number of activated microglias was significant greater in the Alzheimer's disease patients than that of the controls (P < 0.01). The activated microglias displayed altered size and morphology, and was therefore, categorized into three subtypes as primed, enlarged and phagocytic microglias. The numbers of primed, enlarged and phagocytic microglias were 5.4 +/- 0.87, 11.5 +/- 1.25, and 3.4 +/- 0.32 microglia/mm2 and represented 26.6%, 56.65%, and 16.75% of all activated microglias respectively. The number of TUNEL positive apoptotic neurons was significantly greater in Alzheimer patients than that in the control group (P < 0.05). There was a close relationship between the apoptosis of neurons and the activation of microglias (P < 0.01). The activated microglias were differentially distributed among four different plaque types in Alzheimer patients. Many primed (42.3%) and most of the enlarged and phagocytic microglias (56.2% and 70.6%) were present in the diffuse neuritic plaques.

CONCLUSIONSHyperplasia and activation of microglias are a common phenomena in AD and may play an important role in its pathogenesis. There is a close relationship between the apoptosis of neurons and activation of microglias. The activation of microglias may play a key pathogenic role in senile plaque formation and progression of Alzheimer disease.

Aged ; Alzheimer Disease ; etiology ; pathology ; Amyloid beta-Peptides ; analysis ; Apoptosis ; Cell Count ; Cell Differentiation ; Humans ; Immunohistochemistry ; In Situ Nick-End Labeling ; Interleukin-1 ; analysis ; Microglia ; chemistry ; classification ; pathology ; Middle Aged ; Phagocytes ; pathology