ObjectiveTo study the differential expression of signal transduction related gene between the tunica media in human abdominal aortic aneurysms (AAA) and normal aorta, and inspect their role in the etiology of abdominal aortic aneurysms.MethodsGene chip technique was applied to study the differential expression of signal transduction related gene in human AAA, then screening some significant differential expressed genes for further research by RT-PCR, Western Blot, and immunohistochemistry.ResultsForty-five differentially expressed genes related to cellular signal transduction were detected accounting for 1.09% of the chip′s total 4?096 genes,among them 28 genes were up-regulated and 17 down-regulated in aortic aneurysm. ASK1, ERK1 gene were found up-requlated in aortic aneurysm.ConclusionsDifferential expression of signal transduction gene could play a key role in the occurrence of AAA.

Objective To summarize the experience of partial splenctomy in patients with traumatic spleen rupture.Methods Patients were assigned to spleen conservation group in which 59 patients underwent partial splenectomy,control group Ⅰ in which 60 patients underwent splenectomy and control group Ⅱ in which 60 patients underwent gastroinestinal operation. Immunologic function and filtration of red blood cell were detected in the three groups during the preoperation and the first month,the first year postoperation respectively.Results There was no significant difference between the spleen conservation group and the gastroenteric operation group.But IgM,NK-cell activity,T lymphocyte subpopulation and T lymphocyte transformation rate in the spleen conservation group were significantly higher then those in the splenectomy group (P

This paper reports the methods of inducing animal model of abdominal aortic aneurysm(AAA) and the research on etiology.The formation of AAA is the result of cooperation of multifactors such as genetic and biochemistric facfors.Different animal models of AAA can provide a possibility in researching the etiology and pathogenesis of AAA etc, and useful in judging the therapy and prognosis .

ObjectiveTo observe the effect of the designed cervical orthosis(CO) and the finished collar in the treatment of vertebroarterial cervical spondylopathy.Methods70 patients with vertebral artery type cervical spondylosis were divided into designed cervical orthosis group (35 casese) and the finished collar group (35 casese) separately.ResultsThe excellent and good rate of the designed cervical orthosis group was 94-3% while that of the finished collar group was 71-4%. There was significant difference between the two groups after 4 weeks of treatment(P<0-01).ConclusionThe designed cervical orthosis(CO) in the treatment of vertebroarterial cervical spondylopathy is more effective than finished collar.

Objective Repair of aortic arch aneurysm is technically demanding and usually requiring complex circulatory management. Operative morbidity and mortality may be prohibitive with traditional surgical intervention. We described our experience with 5 hybrid endovascular procedure for aorta repair with different kinds of bypass followed by concomitant placement of stent graft in the aorta. Methods We retrospectively reviewed the clinical data of 5 consecutive patients presenting with aortic aneurysm or dissection from 2007 to 2008 treated by the hybrid aorta repair. Complete surgical rerouting of the supra-aortic vessels was followed by endovascular repair of aortic arch aneurysm with a Zenith TX2 stent graft. Hybrid left carotid-subclavian bypass with Zenith stent graft deployment covering the ostium of the LSA was performed in a Debakey type Ⅲ aortic dissection case. Procedures were successfully completed with exclusion of the aortic aneurysm. All stent grafts were deployed retrograde from the femoral artery in these patients. Results Technical success with complete aneurysmal exclusion was achieved in all patients (100%). At a follow-up period of 2-10 months, there was no incidence of endoleak. Documented perioperative neurelogic events did not occurred in all patients. Postoperatively one patient suffered from ARDS and cardiac failure and recovered. One patient died of myocardial infarction. Conclusions Hybrid arch repair provides an alternative to patients otherwise considered prohibitively high risk for traditional open arch and thoracoabdominal aorta repair.

Objective To study the clinical and neuroimaging features of top of the basilar artery syndrome(TOBS).Methods The clinical and neuroimaging features of 63 cases with TOBS were analyzed retrospectively.Results Main causes of TOBS were atherosclerosis and embolisms.Vertigo and disturbance of consciousness and movement of the eye-ball,abnormality of pupils,partial blindness were the main clinical features.The most important imageologic feature of TOBS was infarction of thalami or midbrain,which was frequently complicating with infarction in other locations.Conclusion TOBS has simultaneously more than two infarction lesions,either superior or inferior tentorium of cerebellum with many complications and poor prognosis.MRI is more sensitive than CT.Early MRI examination,systemic and comprehansive treatment may improve the prognosis of the TOBS patients.

Objective To describe the understanding level of disease of children with leukemia and their ways of obtaining disease information in order to help nurses and parents to select appropriate content and manners to communicate with children about disease information.Methods In-depth interviews were conducted with 25 children' parents using a descriptive qualitative research method,and the data were analyzed using content analysis.Results Children during remission stage of leukemia had different understanding levels of their disease.Ways of children with leukemia to obtain disease information was correlated with their mental maturity.Conclusions Disease information should be told according to children's age,disease course and level of thinking,and health professionals and parents could provide appropilate ways of obtaining information for children on basis of their mental maturity.

Objective To evaluate arterial reconstruction of traumatic iliac-femoral artery pseudoaneurysm in parenteral drug abusers.Methods Data of 21 consecutive patients who presented with iliac-femoral artery pseudoaneurysm with the diameter ranging from 3.0 cm～7.5 cm secondary to parenteral drug abuse from 2004 to 2007 were analyzed.Fifteen patients were male and the median age was 3 1.3 years.the aneurysm involved the common femoral artery and distal external iliac artery.Autologous greater saphenous vein was used as a graft for arterial reconstruction after iliac-femoral artery pseudoaneurysm excision through a single curved inguinal incision.Results The surgical procedure was successful in all 21 eases without intraoperative mortality and severe complications.All the 21 patients were free of postoperative claudication symptoms except 1 case with preoperative popliteal artery stenosis.Wound infection and tissue fistula developed in one ease.Ingunal incisional hematoma and cutaneous abnormal sensation developed in one each eases.Conclusions The use of autologous greater saphenous venous grafts for aaefial reconstruction after false aneurysm excision in drug abusers is safe and effective.The harvest of the greater saphenous vein is precondition of this precedure.These preliminary results indicate that the implementation of this technique offers advantages compared with the artery ligation alone without revascularization,that is frequently associated with later intermittent claudication.

AIM:To investigate the effects of 17β-estradiol on the expression of macrophage migration inhibi-tory factor ( MIF) in cultured endometrial stromal cells from endometriosis .METHODS:Immunohistochemistry was used to identify the endometrial stromal cells .The expression of MIF at mRNA and protein levels was assessed by RT-PCR and Western blotting .RESULTS:Elevated expression of MIF at mRNA and protein levels was observed in the cultured endom-etrial stromal cells treated with 17β-estradiol.In endometrial stromal cells from the women with endometriosis , the level of MIF up-regulation by 17β-estradiol was significantly higher than that in the cells from the women without endometriosis . CONCLUSION:Endometrial stromal cells from endometriosis are more sensitive to 17β-estradiol, which up-regulates the expression of MIF and contribute to the pathogenesis and progression of endometriosis .

Objective To study the differential expression of cell adhesion molecule(CAM) genes in the pathegenesis of AAA. Methods Microarray technique was applied to screen for the differential expression of CAM genes between AAA and normal aortic tissues. Results Three differentially expressed CAM genes were found in AAAs by microarray technique and molecular biology investigation, including VCAM 1, PECAM 1, TSP,with up regulating ratio of 5 683,3 601,57 406,respectively.Conclusion These abnormally up regulated CAM genes found in AAA might participate in the development of abdominal aortic aneurysms.