Objective To assess the safety and efficacy of danhong injection combined with edaravone for acute cerebral infarction ( ACI) . Methods To search CBM、CNKI、VIP、WF、MEDLINE for randomized controlled trials ( RCTs) of related articles from inception to November 2014.After assessed the quality of studies included,RevMan5.2 software was used to analyze data. Results 23 studies,were included and 2 001 patients were involved.The results showed that the effective rate of danhong injection combined with edaravone for ACI was better than that of control group with statistical significance [ RR=1.23, 95%CI (1.17,1.29), P<0.000 01].Meta-analysis on the improvement of neurological deficit and activities of daily living also showed a better effectiveness of experimental group than control group with statistical significance [MD=-3.09,95%CI(-3.68,-2.50),P<0.000 01];[MD=15.15,95%CI(12.69,17.62), P<0.000 01].But the result showed no statistical significance in safety evaluation of two groups [RR=1.88,95%CI(0.76,4.63),P=0.17]. Conclusion Although the results support the conclusion that danhong injection with edaravone is safe and superior to either danhong injection or edaravone or conventional treatment, further large sample and high quality randomized controlled trials should be carried out.

Objective To summarize the experience of partial splenctomy in patients with traumatic spleen rupture.Methods Patients were assigned to spleen conservation group in which 59 patients underwent partial splenectomy,control group Ⅰ in which 60 patients underwent splenectomy and control group Ⅱ in which 60 patients underwent gastroinestinal operation. Immunologic function and filtration of red blood cell were detected in the three groups during the preoperation and the first month,the first year postoperation respectively.Results There was no significant difference between the spleen conservation group and the gastroenteric operation group.But IgM,NK-cell activity,T lymphocyte subpopulation and T lymphocyte transformation rate in the spleen conservation group were significantly higher then those in the splenectomy group (P

Objective To investigate the effects of 2 450 MHz microwave irradiation on the proliferation of cultured mouse fibroblasts in vitro, and the related gene and protein expressions. Methods Cells from mouse skin were directly radiated with microwaves of different intensities for different periods. The proliferation of fibroblasts was assayed by the MTT method, and the effects of microwave radiation on the fibroblast cell cycle were measured by flow eytometry. The mRNA expression of types Ⅰ and Ⅲ procollagen was detected by real-time RT-PCR. Anti-phosphoryl- ation extracellular-regulated kinase (ERK-1/2) antibody was introduced in immunofluorescence staining analysis to observe any changes in the phosphorylation of fibroblast protein ERK. Results ① 5 W/cm2 or 1.0 W/cm2 irradia- tion for 5 min, 15 min, or 30 min did not significantly decrease fibroblast proliferation, but irradiation at 5 W/cm2 for more than 5min caused a significant decrease in fibroblast proliferation. ②After 5 W/cm2 irradiation for 5 min, the percentage of cells in the G0/G1phase was significantly increased, and ERK was activated immediately after irradia- tion. ③The mRNA expression of type Ⅰ procollagen was down-regulated after microwave irradiation, and the magni- tude of the decreased expression correlated positively with the duration of irradiation. Thirty minutes of microwave ir- radiation at 1 W/cm2 or 5 minutes at 5 W/cm2 significantly down-regulated the mRNA expression of type Ⅰ procolla- gen and the ratio of types Ⅰ and Ⅲ. Conclusion High-intensity microwaves may inhibit the proliferation of fibro-blasts in a dose-dependent manner in vitro and down-regulate procollagen mRNA expression, which might be achieved by activating mitogen-activated protein kinase signal transduction gateways.

Objective To investigate the incidence of osteoarthritis disease, related factor and need of rehabilitation services. Methods 13579 questionnaires were sent randomly to the permanents resident in Xuhui District, and 12419 returned. Results The morbidity of cervical syndrome, lumbar diseases, knee osteoarthritis were 22.74%, 31.45%, and 23.37%, and more in females (P＜0.01). 23.12%, 16.59%, 20.12% patients with cervical syndrome, lumbar diseases, knee osteoarthritis accepted rehabilitation respectively, and 28.08%,36.05%, 34.18% accepted self-exercise. The top 3 complications were hypertension, cardiovascular diseases, and gastropathy. Conclusion It is important to improve the rehabilitation intervention and health education to the females and the elders, as well as the community health services and the community general practice team.

Objective To investigate the regenerating effect of muscle satellite cells autografted onto mus-cles injured by chronic compartment syndrome (CCS). Methods Twenty-four adult rabbits were randomly divided into an experimental group (model-transplantation), a non-graft group (model) and a control group (transplanta-tion). The CCS model was established in the experimental and non-graft groups. Transplantation was done in the ex-perimental and control groups. Satellite cells from the experimental and control group rabbits were isolated and then proliferated in vitro. The specific protein was identified by immnochemistry before engrafting. Then the 4',6-diamidi-no-2-phenylindole-tagged satellite cells were transplanted back to the original soleus muscles. After transplantation,the proliferation and differentiation of the satellite cells in vivo was observed and morphological changes at the site of the injury were compared by HE staining. Results At the 28th day after grafting, the satellite cells from the com-pressed soleus muscles in the experimental group had increased significantly, whereas those in the control group had remained the same. In both the graft group and the non-graft group, HE staining showed a large cluster of myofibers and interstitial fibers necrosed in the compressed muscle, while the skeletal muscle fibers and interstitial fibers were in-tact in the control group. At the 28th day after engrafting, the engrafted muscle showed much repair; but the non-en-grafted muscle demonstrated dominant fibrosis. The morphology of the skeletal muscle in the control group was normal.Conclusions Autografting of muscle satellite cells after a small amount of expansion in vitro could improve their regen-eration efficiency for repairing a large cluster of damaged myofibers caused by chronic compartment syndrome.

Objective To investigate the influence of systematic health education on the disease cogni-tion and behavior of treatment compliance in patients with infectious diseases. Methods Patiens with infec-tious diseases in our department were divided into the control group(142 patients) and the experimental group (136 patients). The control group received common health education, the experimental group was given addi-tional knowledge education about infectious disease based upon routine education. The two groups were inves-tigated before and after intervention by adopting self-designed investigation scale.The cognition rate of disease and the rate of behavior of treatment compliance were compared by χ2 test. Results The cognition rate of disease and the rate of behavior of treatment compliance in the experimental group were higher than those of the control group. Conclusions Systematic health education can not only increase degree of disease cogni-tion but also improve behavior of treatment comphanee in patients with infectious diseases.

Objective Practice and exploration in relation to development with large general hospital and community-based care of rehabilitation programs.Methods Utilization of advantage resources for competition and effective combination of community-based health care system can develope community-based care of rehabilitation programs.Results Feasibile plan finished in the primary and community-based health care system and technology resource can provide a theoretical basis for sustainable development.Conclusion Education of skill and rehabilitation guidance can enhance service rehabilitation in community-based.

The Community Health Care Centre of Xujiahui, Shanghai tries to integrate the community-based rehabilitation for orthopedic chronic diseases into the primary health care system within the general practices. This paper would discuss its features, problem, and prospect.

Objective:Recent studies have shown that in contrast to decrease in distal gastric adenocarcinoma (DGA), incidence of adenocarcinoma of the esophagogastric junction (AEG) has increased noticeably in numerous counties. However, the reasons remain unclear. This study evaluated the possible differences in the expression of KLF4, SP1, and Cyclin D1 in AEG and DGA, and explored the potential carcinogenesis of AEG. Methods:Immunohistochemistry was performed on paraffin-embedded tissues to evaluate the pu-tative differences in the expressions of KLF4, SP1, and Cyclin D1 at protein level between AEG (n=58) and DGA (n=47). The patholog-ical significance of these markers between the two groups was also compared and analyzed. Results:The percentage of positive KLF4 expression was significantly lower in DGA than in AEG (P<0.05). Lower KLF4 expression was found both in well-or moderately dif-ferentiated cases and in poorly differentiated cases with DGA compared with their AEG counterparts (P<0.05). However, positive stain-ing for SP1 was significantly higher in DGA (P<0.05). No significant difference was found in the expression of Cyclin D1 between the two groups. Further analysis showed that in DGA, the positive expression of KLF4, SP1, and Cyclin D1 were significantly correlated with lymph node metastasis. In AEG, only Cyclin D1 expression was correlated with lymph node metastasis (P<0.05). No correlation was found among the expression of KLF4, SP1, and Cyclin D1 in AEG. In DGA, KLF4 was inversely correlated with SP1 and Cyclin D1 (r=-0.334 and r=-0.341, respectively, P<0.05), and SP1 was positively correlated with Cyclin D1 expression (r=0.340, P<0.05).Conclusion:Different expression patterns and clinicopathological significance of KLF4, SP1, and Cyclin D1 were observed between AEG and DGA, suggesting the putative difference in the carcinogenesis and progression of AEG and DGA.