Parkinson's disease(PD) is a kind of degenerative disease caused by both genetic and environ-mental factors. Concerning these two factors, various animal models for parkinson's disease were made for the study. Transgenic animal models were made by the method of microinjection, electronic perforation, particle bom-bardment, virus vector transfection and so on, which can express exogenous target gene. These models imitated the process of Parkinson's desease induced by virulence gene and provided a powerful tool for investigation on e-tiopathogenesis, gene therapy and medical intervention. In this article, we reviewed the progress of genetic models associated with PD and theirs contribution to PD research.

Objective To develop stable cultures of human umbilical cord vascular endothelial cells(ECs)and smooth muscle cells(SMCs)in order to investigate the effect of mycophenolic acid(MPA)on proliferation and collagen Ⅰ production of SMCs.Methods From Sep.2002 to Sep.2003,ECs were cultured from human umbilical cord veins,and SMCs from arteries.Productions of endothelial cells conditioned medium(ECCM)was obtained from serum free Dulbecco's modified eagle medium(DMEM)with or without MPA(0,0.31,1.25,2.50,5.00,10.00 ?g?mL -1).Proliferation of SMCs was performed with 3H-thymidine incorporation scintillation,and collagen Ⅰ production of SMCs was measured with enzyme-linked immunosorbent assay.Results (1)The 3H-thymidine incorporation increased significantly(P

OBJECTIVE This study is to investigate the effects on nasopharyngeal carcinoma cell lines(CNE-2Z)induced by As2O3 and its possible mechanisms.METHODS CNE-2Z cells were treated with various concentrations of As2O3 for different times.The IC50 values were detected by trypan blue stain assay to choose the optimal concentration of As2O3.Cell cycle redistribution was analyzed by flow cytometry.The expression levels of p16、 RASSF1A、DNMT 3B mRNA were assessed by RT-PCR.RESULTS The suppression of cell proliferation by As2O3 was time and dose-dependent.After being treated with different concentration of As2O3,the IC50 values of As2O3 were(1.50?0.05),(1.09?0.13),(0.65?0.04)?mol/L at 24,48,and 72h,respectively.As2O3 also arrested CNE-2Z cells in G2/M phase of cell cycle.After CNE-2Z cells being treated with As2O3,the expression of RASSF1A mRNA were up-regulated(P

Abstract A rapid gas chromatographic ( GC ) method was established for the determination of short-chain fatty acids ( SCFAs ) in human feces. Feces samples were directly extracted by 1% HCl-75% ethanol solution, and then centrifuged at high speed for GC analysis. The chromatographic separation was performed on a DB-FFAP capillary column ( 30 m í 0 . 25 mm í 0 . 25 μm ) with a temperature program ( initial temperature at 50℃ held for 1 min, ramped to 190℃ at 10℃/min ) . The injection port temperature was 250℃ with split ratio at 50:1 . The carrier gas was high purity nitrogen with a constant linear velocity at 1. 0 mL/min. A flame ionization detector was employed to quantify SCFAs. The proposed method had been certified by systematic method validation, and used to determine feces samples. Subsequently, the health volunteer and colorectal cancer patient groups could be distinguished successfully by multivariate statistical analysis. Compared with health volunteers, the acetic acid and butyric acid of feces from colorectal cancer patients were reduced obviously, indicating that SCFAs particularly butyric acid could be considered as candidate markers for colorectal cancer diagnosis. In summary, this study provides a rapid method for the determination of SCFAs in feces form health volunteers and colorectal cancer patients. The method had a prospect for screening and diagnosing colorectal cancer rapidly.

OBJECTIVE To explore the relationship between allergic rhinitis and IFN-?, IL-12. METHODS The expression of IL-12 was detected by in situ hybridization and enzyme-linked immunosorbent assay in 37 patients with allergic rhinitis and 12 control samples. IFN-? was detected by ELISA. RESULTS The positive ratios of IL-12 in PBMC were 0.13?0.03 and 0.77?0.15 in allergic rhinitis group and control group respectively. The concentrations of IL-12 were (13.7?2.4 ) pg/L and (52.9?8.4 ) pg/L in allergic rhinitis group and control group respectively. The concentrations of IFN-? were (11.2?2.1 ) pg/L and (51.8 ?6.4) pg/L in allergic rhinitis group and control group respectively. CONCLUSION The down-regulation of IL-12 and IFN-? was related to allergic rhinitis, and hyper-function of Th2 cells may play an important role in the pathogenesis of allergic rhinitis.

Objective: In order to detect the effect of bcl-xs on camptothecin-induced apoptosis in human nasopharyngeal carcinoma CNE-2Z cells in vitro.Methods:bcl-xs gene-bearing mammalian expression vector(pcDNA3xs)was transfected into CNE-2Z cells using LipofectAmine.The expression of bcl-xs was determined with western blot.Cells which were transfected with native pcDNA3 vector were used as control.Apoptotic cells were detected with flow cytometry after exposure to camptothecin for 24h.Results:Cell clone(CNE-2Zxs)with stable expression of bcl-xs was obtained as confirmed with western blot.Results from flow cytometry analysis showed a significant increase of apoptotic cells in CNE-2Zxs as compared with CNE-2Zneo after treatment with the same dose of camptothecin.Conclusion:Exogenous bcl-xs expression sensitized nasopharyngeal carcinoma CNE-2Z cells to camptothecin-induced apoptosis.

OBJECTIVE: To investigate the effect of arsenic trioxide (As2O3) combined with cisplatin on expression of RASSF1A in nude mice with human nasopharyngeal carcinoma xenograft. METHOD: The models of human poorly differentiated nasopharyngeal carcinoma in nude mice were established and randomly divided into four groups, control group (NaCl group), As2O3 group, DDP group and As2O3 + DDP group. The expression of RASSF1A mRNA and protein were detected by Real-time RT-PCR and immunohistochemistry respectively. The methylation rate of RASSF1A promoter CpG islands was analyzed by HRM. RESULTS: Experimental groups could obviously inhibit the growth of tumor and up-regulate the expression of RASSF1A. The methylation rate of RASSF1A in transplanted tumors in experimental groups was lower than the control group. Especially As2O3 combined with DDP were superior to the single drug use. CONCLUSION As2O3 inhibits the growth of human nasopharyngeal carcinoma cell strain CNE2 xenograft in nude mice and increases mRNA expression of RASSF1A. As2O3 inhibits the malignant phenotypes of human nasopharyngeal carcinoma cells and reverses hypermethylation of RASSF1A.
Animals ; Arsenic Trioxide ; Arsenicals ; pharmacology ; Carcinoma ; Cell Line, Tumor ; Cisplatin ; pharmacology ; DNA Methylation ; drug effects ; Humans ; Mice ; Mice, Nude ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; genetics ; pathology ; Neoplasm Transplantation ; Oxides ; pharmacology ; Tumor Suppressor Proteins ; genetics ; Xenograft Model Antitumor Assays

[ABSTRACT]OBJECTIVETo study the effects of Arsenic Trioxide on the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, and explore the possible mechanisms of the antitumor effect. METHODSThe models of human poorly differentiated nasopharyngeal carcinoma in nude mice were established and randomly divided into 4 groups (control group, As2O3 group, DDP group and As2O3+DDP group). The antitumor effect of every group was studied. Pathological changes of tumor tissues dyed by HE staining were observed with light microscope. The pathological changes of the heart, liver, lung and kidney from nude mice were also observed by HE staining. Blood routine test was examined. Expression of PCNA and Ki-67 were detected by immunohistochemistry method.RESULTSThe growth of tumor in As2O3, DDP and As2O3+DDP were obviously inhibited. No pathological changes and metastases were found in heart, liver, lung and kidney of the nude mice. No obvious toxicity of the treatment to the hematopoietic systems in the nude mice was observed. The expression of PCNA and Ki-67 were down-regulated.CONCLUSIONAs2O3 can inhibit the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, the mechanism of which might be related to the down-regulation of PCNA and Ki-67.

Objective:To investigate the purification of antibacterial effective fraction of Syringae folium by macroporous resins. Methods:Static adsorption and desorption tests were carried out to screen the macroporous resins. The desorption experiment was per-formed on the selected D101 resin to optimize the separation process. The effects of resin amount, diameter length ratio, elution flow rate, elution solution concentration and volume were studied. Results:The optimal conditions were as follows:the elution solution was 55% ethanol, the adsorption flow rate was 1 BV·h-1 , the elution flow rate was 5 BV·h-1 , 6 BV 25% ethanol was used to eliminate impurity and 8 BV 55% ethanol was used to elute to obtain the effective fraction. Conclusion: The content of antibacterial effective component is above 65% after purified by D101 resin, indicating that the present method is suitable for large-scale preparation of anti-bacterial effective fraction of Syringae folium.

Objectives To evaluate the lung function in patients of severe secondary hyperparathyroidism caused by uremia and to analyze related factors.Methods The pulmonary function of 70 patients with uremia ( 40 patients with severe secondary hyperparathyroidism, 30 MHD patients without SHPT) and 30 healthy people was determined.Correlative analysis was performed between parameters such as pulmonary function indexes and clinical or laboratory parameters. Results Lung function was significantly different between patients with uremia and healthy subjects(P<0.05, respectively). A number of lung function indicators were negatively correlated with iPTH,C reactive protein( CRP) ,dialysis age but were positively correlated with hemoglobin (Hb) and albumin(Alb) (P <0.05).Conclusion Patients with SHPT show impaired lung function.IPTH, dialysis age, Hb, Alb and CRP can be used as important indicators of lung function.