Objective To investigate the intracranial vascular stenosis and risk factors in different types of ischemic stroke. Methods 525 patients with ischemic stroke were assessed by a transcranial Doppler. Lipids including total cholesterol (CHO),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG) were examined. Results Intracranial vascular stenosis in diabetic and non-diabetic groups of patients,hypertension and non-hypertension groups of patients showed significantly difference (P

According to the traditional point of view,CT and lumabar puncture are the main means in the diagnosis of subarachnoid hemorrhage(SAH).The values of conventional MRI T1-weighted imaging(T1WI)and T2 weighted imaging(T2WI)are very little in the diagnosis of SAH.With the development of MRI technique and the research and application of novel MRI sequences,the value of MRI are gradually recognized and affirmed in the diagnosis of SAH.and has shown its superiority.MRI isn't inferior to CT in the diagnosis of acute SAH.evidently superior to CT in the diagnosis of subacute,chronic and atypical SAHs and superior to CT in the diagnosis of the complications of SAH.MRI is valuable in screening for intraeranial aneurysms and postoperative follow-up.

Objective To establish a fingerprint analysis method for the medicinal slices of Radix et Rhizoma Rhei.Methods HPLC with ZORBAX Eclipse XDB-C18 was applied.The chromatographic conditions were as follows:acetonitrile-0.1 %H3PO4 solution(gradient alution) as a mobile phase and detection wavelength at 254 nm,flow rate at 1 mL?min-1,and column temperature being 40 ℃.Results Twenty-five common peaks were separated from 10 batches of medicinal slices of Radix et Rhizoma Rhei.Conclusion The method is reliable and accurate,and can be used as a quality control method for the medicinal material of Radix et Rhizoma Rhei.

AIM:To establish the method of fingerprint analysis on Shuangbai Powder(Radix et Rhizoma rhei,Cacumen platycladi,Cortex phellodendri amurensis,Herba lycopi and Herba menthae) to distinguish the characteristic fingerprint.METHODS:HPLC with ZORBAX Eclipse XDB-C18 was used,acetonitrile-0.1% H3PO4 solution(gradient elution) as a mobile phase and detection wavelength at 254 nm,flow rate was 1 mL/min,and column temperature was 40 ℃.RESULTS:Thirty-seven common peaks were separated from 10 batches of Shuangbai Powder.The characteristic peaks were the summation,22 peaks were from Radix et Rhizoma Rhei,6 peaks were from Cortex phellodendri Amurensis,4 peaks were from Cacumen Platycladi,3 peaks were from Herba Lycopi,and 2 peaks were from Herba Menthae,there was one new characteristic peak.CONCLUSION:Ten peaks gathered from Shangbai Powder consist of rhein,emodin,chrysophanol,aloe-emodin,physcion,gallic acid,berberine hydrochloride,palmatine;quercitrosid,and linarin.

AIM: To establish a method of fingerprint analysis on Cacumen Platycladi. METHODS: HPLC was adopted with ZORBAX Eclipse XDB-C18,acetonitrile-0. 1% H3PO4 solution (gradient alution) as a mobile phase and detection wavelength at 254 nm,flow rate was 1 mL/min,and colum temperature was at 40 ℃. RESULTS: Eleven common peaks were separated from 14 batches of Cacumen Platycladi. As compared with standard sample,quecitroside and quecitin were checked out. CONCLUSION: The method is reliable,accurate and can be used as a quality control method for Cacumen Platycladi.

Objective To evaluate development of gastro-esophageal reflux (GER) during laparoscopic surgery in lateral jack-knife position under general anesthesia through comparing with reverse Trendelenburg/ Trendelenburg position in the patients lying supine.Methods Ninety patients of both sexes,aged 18-64 yr,of ASA physical status Ⅰ or Ⅱ,with body mass index of 18-30 kg/m2,scheduled for elective laparoscopic surgery under general anesthesia,were randomly divided into 3 groups (n =30 each):lateral jack-knife position group (group L),Trendelenburg position group (group T) and reverse Trendelenburg position group (group Tre).Anesthesia was induced with midazolam,sufentanil,propofol and cisatracurium besylate and maintained with propofol and remifentanil given by target-controlled infusion.A pH-sensitive probe was inserted through nose into the lower esophagus and pH value was continuously recorded until 1 min after extubation.GER was defined as pH value ≤ 4 lasting for ≥ 1 min in the lower esophagus during surgery.The development of GER during surgery and the lowest pH value in the lower esophagus when GER developed were recorded.Results Compared with group Tre,the incidence of GER (27％) and total number of times GER had occurred were significantly increased in group L,and no significant changes were found in the indices mentioned above in L and T groups.When GER developed,the lowest pH value in the lower esophagus was 2.1 ± 1.3,2.6 ± 1.2 and 3.5 in L,T and Tre groups,respectively.Conclusion The incidence of GER is 27 ％ during laparoscopic surgery when the patients are in lateral jack-knife position and it is higher than that obtained with reverse Trendelenburg position in the patients lying supine.

AIM:To establish the method of fingerprint analysis on Shuangbai Powder(Radix et Rhizoma rhei,Cacumen platycladi,Cortex phellodendri amurensis,Herba lycopi and Herba menthae)to distinguish the characteristic fingerprint.METHODS:HPLC with ZORBAX Eclipse XDB-C_(18) was used,acetonitrile-0.1% H_3PO_4 solution(gradient elution)as a mobile phase and detection wavelength at 254 nm,flow rate was 1 mL/min,and column temperature was 40℃.RESULTS:Thirty-seven common peaks were separated from 10 batches of Shuangbai Powder.The characteristic peaks were the summation,22 peaks were from Radix et Rhizoma Rhei,6 peaks were from Cortex phellodendri Amurensis,4 peaks were from Cacumen Platycladi,3 peaks were from Herba Lycopi,and 2 peaks were from Herba Menthae,there was one new characteristic peak.CONCLUSION:Ten peaks gathered from Shanghai Powder consist of rhein,emodin,chrysophanol,aloe-emodin,physcion,gallic acid,berberine hydrochloride,palmatine;quercitrosid,and linarin.

Objective To establish a fingerprint analysis method for Ganzhixiao Decoction. Methods RP-HPLC was applied with Phenomenex Synergi 4?Hydro-RP 80A as the chromatic column,methanol-0.1 %H3PO4 solution (gradient elution) as the mobile phase,detection wavelength at 280 nm,flow rate being 1.0 mL/min,and column temperature at 30 ℃. Results Twenty common peaks were presented in 10 batches of Ganzhixiao Decoction and the positions of 20 characteristic peaks were identified,including 5-hydroxymethyl furfural,chlorogeni C acid,Salvianolic acid B. Conclusion The method is reliable and accurate,and can provide a reference for the research of Ganzhixiao Decoction.

A growing body of evidence demonstrates that vascular factors have involved in the occurrence and development of Alzheimer's disease.This article reviews the research progress of the AD-associated vascular factors from the aspects of AD epidemiology,pathology,clinical research,imaging,and genetics,expecting to investigate the nature of AD from all aspects through vascular factors and finding a new entry point for prevention,diagnosis and treatment of AD.

Objective To study the effects of genistein(GST) on the expression of estrogen receptor ?(ER?) and the expression of c-fos gene in the aorta of ovariectomized rats.Methods Forty female Wistar rats were randomly divided into four groups:sham-operated(control),ovariectomized(OVX),ovariectomized with 17?-E2 replacement(OVX+E2),ovariectomized with genistein replacement(OVX+GST) group.After 8 weeks' replacement therapy,the rats were sacrificed and the expression of ER? and c-fos in the aorta was studied by immunohistochemistry.Results Ovariectomy significantly decreased ER ? expression and increased c-fos expression in aorta;while replacement therapy,GST and E2,attenuated the effect of ovariectomy manifested by increasing ER expression and decreasing c-fos expression in aorta.Conclusion GST may modulate the expression of ER? in the arteries of ovariectomized rats and play a beneficial effects on cardiovascular system which was associated with decreases in expression of c-fos gene in aorta.