Objective To investigate the application effect of indwelling needle puncture in the axillary vein of newborn infants.Methods From September 2015 to February 2016,the venous indwelling needle puncture was given for neonatal transfusion 180 cases,including 90 cases of the axillary vein indwelling needle infusion in the experimental group,and 90 cases of puncture of extremity vein infusion in the control group.The application effect of the 2 groups was compared.Results There were significant differences in terms of indwelling needle shedding,phlebitis,drug extravasation,lien time between the experimental group and the control group (P ＜ 0.05) Conclusions During neonatal indwelling needle puncture infusion process,the axillary vein indwelling needle infusion can prevent the indwelling needle shedding,decrease the incidence of phlebitis and reduce the incidence of drug extravasation,and can prolong the indwelling time.Puncture in the simple operation easy,is easy to learn and no need for special facilities and equipment,only conventional intravenous infusion required material,plus 24g indwelling needle,aseptic transparent dressing,paper tape,economical and practical,suitable for application in neonatal ward.

OBJECTIVE: To establish an HPLC method for the determination of diprophylline in diprophylline tablets. METHODS: The analytical column was C18 and the mobile phase consisted of methanol-water (30∶ 70) at a flow rate of 1.0 mL?min-1. The detection wavelength was 273 nm and the sample size was 20 ?L. RESULTS: The linear range of diprophylline was 8.1～81 ?g?mL-1 (r=0.999 8) with an average recovery rate of 100.01% (RSD=0.21%). CONCLUSION: The method is simple, accurate and precise, and it can be used for the quality control of diprophylline tablets.

Objective To investigate the application effect of the puncture method of indwelling needle by single hand in clinical attendance of pediatrics. Methods From March 2015 to September 2015,578 patients in our pediatrics were randomly and equally divided into two groups. The experiment group were treated with the puncture method of indwelling needle by single hand and the control group received traditional puncturing method. Results There were statistical significances in the success rate of first puncture,degree of comfort,sense of pain,dropping of the indwelling needle,phlebitis,exosmosis of drugs and indwelling time of patients between the two groups(P< 0.05). Conclusion In the process of using indwelling needle in pediatrics,the application of the puncture method of indwelling needle by single hand could improve the success rate of first puncture of venous retention needle,enhance the comfort degree of neonates,relieve the sense of pain,reduce the dropping of the indwelling needle,incidence of phlebitis and exosmosis of drugs,and prolong the indwelling time. The operation of this puncture is simple and easy to do and to learn,without any special equipment. In the venous puncture,the only necessities are 24G retention needle,3M indwelling needle and adhesive tape. Obviously,it is economical and practical,and is suitable for application in clinic.

Objective To explore the immunogenicity of a novel bionic scaffold of nucleus pulposus tissue engineering. Methods The biocompatibility of a subcutaneously implanted scaffold of nucleus pulposus tissue engineering was studied in SD rats by analyzing tissue reactions up to 3 months using histological and ultrastructural methods. The expression of IFN-?, IL-2, IL-4 and IL-10 mRNA was measured by RT-PCR, and the levels of serum antibodies to porcine type Ⅱ collagen were measured by ELISA. Results There was less inflammatory reaction in rats induced by subcutaneously implanted scaffold. By degrees, a granulation tissue had developed within the implant, which had disappeared by 3 months. The expression of IFN-?, IL-2 mRNA by RT-PCR was of no changes. But the expression of IL-4 and IL-10 mRNA increased, which meant the implant induced Th cell into Th2 cell and the induction inhibited the inflammatory reaction. No antibodies to porcine type Ⅱ collagen were found in the sera of implanted rats. Conclusion There was less immunogenicity in rats induced by implanted scaffold of nucleus pulposus tissue engineering.

Language,as a cartier of specified social culture,is closely and complementarily associated with it.Considering the especial education targets of medical universities and colleges,the present curriculum and the trend of international communication,College English is obliged to focus on humanistic value and necessarily plays its constructive role in heightening students' humanistic quality.

Recent clinical studies have shown that collagen peptides may reduce joint pain and the dependence on analgesics,and enhance sport ability in athletes.Therefore,collagen peptides may be a safe nutrient supplement for prevention and mitigation of joint pain in athletes.

Angiotensin II ; metabolism ; Animals ; Male ; Muscle, Skeletal ; Muscular Atrophy ; etiology ; metabolism ; pathology ; Rats ; Rats, Sprague-Dawley

Factors that may influence vitamin D nutritional status include latitude,season,age,sex,race,skin color,clothing,cultural practices,dietary nutrition,and living conditions.This article reviews the effects of these factors on vitamin D status and recommends that any high-risk people of vitamin D deficiency should take vitamin D supplementation.

Objective To evaluated the sexual psychology changes and marriage quality for ovarian cancer patients after operation and supply selective psychological nursing intervention for patients and their family members.Methods Self-designed questionnaire was used to investigated ovarian cancer patients under 60 years old one month after operation to understand the sexual psychology changes and its related factors.Nursing intervention was adopted according to different conditions of patients.Results Factors that influenced the sexual psychology of patients included:age,education degree,chemotherapy process,clinical symptom,anxiety and depression status,marital status and degree of nursing intervention.Conclusions Sexual psychology Was influenced by many factors.We should adopt various kinds of nursing intervention to promote the physical and mental health of patients,facihtate the recovery of sexual function and improve their life quality.