OBJECTIVE: To establish an HPLC method for the determination of diprophylline in diprophylline tablets. METHODS: The analytical column was C18 and the mobile phase consisted of methanol-water (30∶ 70) at a flow rate of 1.0 mL?min-1. The detection wavelength was 273 nm and the sample size was 20 ?L. RESULTS: The linear range of diprophylline was 8.1～81 ?g?mL-1 (r=0.999 8) with an average recovery rate of 100.01% (RSD=0.21%). CONCLUSION: The method is simple, accurate and precise, and it can be used for the quality control of diprophylline tablets.

Objective To investigate the application effect of the puncture method of indwelling needle by single hand in clinical attendance of pediatrics. Methods From March 2015 to September 2015,578 patients in our pediatrics were randomly and equally divided into two groups. The experiment group were treated with the puncture method of indwelling needle by single hand and the control group received traditional puncturing method. Results There were statistical significances in the success rate of first puncture,degree of comfort,sense of pain,dropping of the indwelling needle,phlebitis,exosmosis of drugs and indwelling time of patients between the two groups(P< 0.05). Conclusion In the process of using indwelling needle in pediatrics,the application of the puncture method of indwelling needle by single hand could improve the success rate of first puncture of venous retention needle,enhance the comfort degree of neonates,relieve the sense of pain,reduce the dropping of the indwelling needle,incidence of phlebitis and exosmosis of drugs,and prolong the indwelling time. The operation of this puncture is simple and easy to do and to learn,without any special equipment. In the venous puncture,the only necessities are 24G retention needle,3M indwelling needle and adhesive tape. Obviously,it is economical and practical,and is suitable for application in clinic.

Objective To investigate the application effect of indwelling needle puncture in the axillary vein of newborn infants.Methods From September 2015 to February 2016,the venous indwelling needle puncture was given for neonatal transfusion 180 cases,including 90 cases of the axillary vein indwelling needle infusion in the experimental group,and 90 cases of puncture of extremity vein infusion in the control group.The application effect of the 2 groups was compared.Results There were significant differences in terms of indwelling needle shedding,phlebitis,drug extravasation,lien time between the experimental group and the control group (P ＜ 0.05) Conclusions During neonatal indwelling needle puncture infusion process,the axillary vein indwelling needle infusion can prevent the indwelling needle shedding,decrease the incidence of phlebitis and reduce the incidence of drug extravasation,and can prolong the indwelling time.Puncture in the simple operation easy,is easy to learn and no need for special facilities and equipment,only conventional intravenous infusion required material,plus 24g indwelling needle,aseptic transparent dressing,paper tape,economical and practical,suitable for application in neonatal ward.

Language,as a cartier of specified social culture,is closely and complementarily associated with it.Considering the especial education targets of medical universities and colleges,the present curriculum and the trend of international communication,College English is obliged to focus on humanistic value and necessarily plays its constructive role in heightening students' humanistic quality.

Objective To know the influencing factors of nursing associations of different provinces and cities undertaking transferred government functions,and to explore the new direction of nursing management system reform based on the association platform.Method 10 experts from 9 provinces and cities were interviewed,and statistics was analyzed by three-level coding combining with QSR NVivo10.0 software.Result 3 themes were collected (internal factors,external support factors and others,such as an independent legal status,financial support,etc).Conclusion Nursing associations,as participants of medical organization and hospital management,must make their own capabilities clear,continuously strengthen their own ability in order to provide better service,and promote the development of medical and health career.

Afferent connections to the nucleus tegmenti dorsalis were studied by means of retrograde axonal transport method of horseradish peroxidase (HRP). HRP was depositted stereotaxically via three approaches: vertical, oblique through the cerebellum or oblique through the cerebrum. The serial sections were treated by 3,3'-diamino-benzidine, benzidine dihydrochloride, diaminobenzidine-cobalt dichloride or tetrame-thylbenzidine respectively.Labeled neurons were observed in following nuclei: nucleus lateralis habenulae,nucleus interpeduncularis, nucleus mammillaris lateralis, nucleus posterior hypothalami, nucleus lateralis hypothalami, substantia grisea centralis mesencephali, nucleus dorsalis raphes, nucleus tractus solitarii, nucleus prepositus hypoglossi, nucleus parafascicularis and nucleus of Darkschewitsch.Then HRP was introduced into nucleus parafascicularis or nucleus prepositus hypoglossi, labeled terminals were also observed within the nucleus tegmenti dorselis.

Objective To synthesize a marine natural product, 24-methylenecholesterol. Methods The target molecule was synthesized in 7 steps using hyodeoxycholic acid as the starting material, 3?-(tetrahydropyran-2-yloxy)-cholest-5-ene-24-ketone as the key intermediate and isopropylation as the key step. Results The target molecule was synthesized with a total yield of 66% and confirmed by MS, IR, 1HNMR and 13CNMR. Conclusion Our synthesis of 24-methylenecholesterol is characterized by easily acquired starting material, short synthetic route, high yield and atom-economic.

Recent clinical studies have shown that collagen peptides may reduce joint pain and the dependence on analgesics,and enhance sport ability in athletes.Therefore,collagen peptides may be a safe nutrient supplement for prevention and mitigation of joint pain in athletes.

Invasive pituitary adenoma is a common pituitary adenoma invading nearby structures such as the sphenoidal sinus, ethmoidal sinus,upper clivus,sellar floor dura and bone. With the development of diagnosis neuroradiology, endocrinology,pathology and microsurgery,dramatic progress has been made on the pathogenesis, pre operative diagnosis and therapy of invasive pituitary adenoma.The development of pathology,molecular biological and medication and surgical therapy of invasive pituitary adenoma in the past few years is discussed in this paper.