Objective To investigate the application effect of the puncture method of indwelling needle by single hand in clinical attendance of pediatrics. Methods From March 2015 to September 2015,578 patients in our pediatrics were randomly and equally divided into two groups. The experiment group were treated with the puncture method of indwelling needle by single hand and the control group received traditional puncturing method. Results There were statistical significances in the success rate of first puncture,degree of comfort,sense of pain,dropping of the indwelling needle,phlebitis,exosmosis of drugs and indwelling time of patients between the two groups(P< 0.05). Conclusion In the process of using indwelling needle in pediatrics,the application of the puncture method of indwelling needle by single hand could improve the success rate of first puncture of venous retention needle,enhance the comfort degree of neonates,relieve the sense of pain,reduce the dropping of the indwelling needle,incidence of phlebitis and exosmosis of drugs,and prolong the indwelling time. The operation of this puncture is simple and easy to do and to learn,without any special equipment. In the venous puncture,the only necessities are 24G retention needle,3M indwelling needle and adhesive tape. Obviously,it is economical and practical,and is suitable for application in clinic.

Objective To investigate the application effect of indwelling needle puncture in the axillary vein of newborn infants.Methods From September 2015 to February 2016,the venous indwelling needle puncture was given for neonatal transfusion 180 cases,including 90 cases of the axillary vein indwelling needle infusion in the experimental group,and 90 cases of puncture of extremity vein infusion in the control group.The application effect of the 2 groups was compared.Results There were significant differences in terms of indwelling needle shedding,phlebitis,drug extravasation,lien time between the experimental group and the control group (P ＜ 0.05) Conclusions During neonatal indwelling needle puncture infusion process,the axillary vein indwelling needle infusion can prevent the indwelling needle shedding,decrease the incidence of phlebitis and reduce the incidence of drug extravasation,and can prolong the indwelling time.Puncture in the simple operation easy,is easy to learn and no need for special facilities and equipment,only conventional intravenous infusion required material,plus 24g indwelling needle,aseptic transparent dressing,paper tape,economical and practical,suitable for application in neonatal ward.

OBJECTIVE: To establish an HPLC method for the determination of diprophylline in diprophylline tablets. METHODS: The analytical column was C18 and the mobile phase consisted of methanol-water (30∶ 70) at a flow rate of 1.0 mL?min-1. The detection wavelength was 273 nm and the sample size was 20 ?L. RESULTS: The linear range of diprophylline was 8.1～81 ?g?mL-1 (r=0.999 8) with an average recovery rate of 100.01% (RSD=0.21%). CONCLUSION: The method is simple, accurate and precise, and it can be used for the quality control of diprophylline tablets.

Objective To know the influencing factors of nursing associations of different provinces and cities undertaking transferred government functions,and to explore the new direction of nursing management system reform based on the association platform.Method 10 experts from 9 provinces and cities were interviewed,and statistics was analyzed by three-level coding combining with QSR NVivo10.0 software.Result 3 themes were collected (internal factors,external support factors and others,such as an independent legal status,financial support,etc).Conclusion Nursing associations,as participants of medical organization and hospital management,must make their own capabilities clear,continuously strengthen their own ability in order to provide better service,and promote the development of medical and health career.

Wavefront-guided laser in situ keratomileusis(LASIK)is onekind of the customized surgeries.It not only can increase the postoperative uncorrected visual acuity(UCVA)and best corrected visual acuity(BCVA),but also can lessen the increase of the higher-order aberration.Meanwhile,wavefront-guided LASIK can improve the scotopic vision,decrease the incidence of the glare and halo and enhance contrast sensitivity(CS).This subject focus on the problems as follows:(1)How to understand the affection that wavefront aberration made on the visual quality after LASIK?(2)How to get the wavefront measurements accurately and choose the surgery adaptation?(3)How to ascertain the gold standard to design the wavefront-guided LASIK?(4)How to transform the wavefront measurements into the optimized surgery scheme?(5)What problems should be paid attention to during the surgery?(6)What results the wavefront-guided LASIK should attain?

Accommodation is a phenomenon that crystalline lens change its refractive power when human eyes see the near objects clearly.The article of A brief talk on ‘ A novel concept of accommodation:Human eyes optical system based on hyperfocal distance-micro zoom'.written by Luo Fu-ming introduces a concept of hyperfocal distance from the camera.Based on the characteristic of hyperfocal distance,the author proposes that there are actually three kinds of hyperfocal distances in human eyes:far-hyperfocal distance,near-hyperfocal distance and accommodative hyperfocal distance.It is important to understand this article that whether the ciliary muscles contract to push the zonules in accommodation,and then the zonules are forced to push the lens equator,or the ciliary muscles contract to relax the tensional zonules,then the posterior surface of the lens moves forward.In view of the anatomic structure,the possibility that the ciliary muscles contract to push the zonule and then the slim zonules push the lens equator is less than that the ciliary muscles contract to relax the tensional zonules and then the posterior surface of the lens moves forward in accommodation.In my opinion,the contraction of the ciliary muscles would startup actively in accommodation.So when the eyes transfer from near vision to distance vision,the ciliary muscles (contracting circular muscles) will return to the relaxed normal state immediately.The crystalline lens will immediately recover to its original relatively flatten state by traction of the zonules,rather than passive compression.Because the crystalline lens is not the startup body but a supporting body in accommodation.The accommodation of human eye is very complicated.During the whole process of accommodation,there are not only contraction of ciliary muscles and increase of refractive power in crystalline lens,but also accompanied reaction of miosis and convergence.So it is difficult to explain the complex activity of human eyes using any extreme,mechanical and single mode.

The development of anti-epidermal growth factor receptor monoclonal antibodies (anti-EGFR McAbs) marked a significant milestone in metastatic colorectal cancer (mCRC) treatment. The addition of anti-EGFR McAb can greatly improve quality of life of mCRC patients and mCRC prognosis and markedly increases the overall survival rate from 6 months to nearly 30 months. KRAS and NRAS mutations contribute to the primary resistance to anti-EGFR therapy and can serve as well-established predictive markers for pa-tient selection. Apart from the RAS family, other molecular alteration in EGFR signaling pathway may also compromise the efficacy of anti-EGFR treatment. In addition, patients who responded to anti-EGFR treatment eventually develop acquired drug resistance within 13 and 18 months. In this review, the mechanisms underlying the primary and secondary resistance to anti-EGFR therapy are summa-rized, and a possible strategy to circumvent drug resistance is proposed. We hope this review can provide compelling evidence, deeper insights, and reasonable guidance to facilitate the precise molecular targeted therapy of mCRC.

Angiotensin II ; metabolism ; Animals ; Male ; Muscle, Skeletal ; Muscular Atrophy ; etiology ; metabolism ; pathology ; Rats ; Rats, Sprague-Dawley

Factors that may influence vitamin D nutritional status include latitude,season,age,sex,race,skin color,clothing,cultural practices,dietary nutrition,and living conditions.This article reviews the effects of these factors on vitamin D status and recommends that any high-risk people of vitamin D deficiency should take vitamin D supplementation.

Objective To explore the immunogenicity of a novel bionic scaffold of nucleus pulposus tissue engineering. Methods The biocompatibility of a subcutaneously implanted scaffold of nucleus pulposus tissue engineering was studied in SD rats by analyzing tissue reactions up to 3 months using histological and ultrastructural methods. The expression of IFN-?, IL-2, IL-4 and IL-10 mRNA was measured by RT-PCR, and the levels of serum antibodies to porcine type Ⅱ collagen were measured by ELISA. Results There was less inflammatory reaction in rats induced by subcutaneously implanted scaffold. By degrees, a granulation tissue had developed within the implant, which had disappeared by 3 months. The expression of IFN-?, IL-2 mRNA by RT-PCR was of no changes. But the expression of IL-4 and IL-10 mRNA increased, which meant the implant induced Th cell into Th2 cell and the induction inhibited the inflammatory reaction. No antibodies to porcine type Ⅱ collagen were found in the sera of implanted rats. Conclusion There was less immunogenicity in rats induced by implanted scaffold of nucleus pulposus tissue engineering.