Chimeric antigen receptor T-cell (CAR-T) technology is based on genetic modification technology to express T-cell expression tumor specific chimeric antigen receptor bind tumor antigen in an antigen-dependent anti-MCH way. Single chain antibody fragment (scFv) of tumor-associated antigen (TAA) combines with up-stream activating sequence of T-cell in vitro. The forming recombinant plasmid transfects the purified and large scale proliferating T-cell in vitro by transfection technique. This process starts and activates specific killing reaction of tumor. The clinical application of cell therapy shows high efficiency and good anti-tumor effect in treatment of malignant neoplasm, such as leukemia, lymphoma, melanoma, which made CAR-T become the mainstream method of cell therapy.

TORCH infection during pregnancy is a group of infectious diseases resulting from viruses and other microorganisms. According to the past over 20-year experiences in serum screening in China, several infections which should be performed in the first trimester, or even much earlier in pregnant period, are hepatitis B, syphilis, HIV, and these three infections are confirmed to be harmful to the fetus or infants and also diagnostic methods and treatment are available. Screening for rubella should be better performed before conception and no specific management is required with IgM+, while immunization is recommended before pregnancy with IgM-. Screenings for cytomegalovirus, toxoplasmosis and herpes virus are not recommended. Scnography is warranted if fetal abnormalities were resulted from the above infections and amniocentesis or cordocentesis might be required for further investigations.

Objective: To explore a novel method for cultivation of the endothelial cells(EC) from infantile hemangiomas (IH) in vitro and observe the biological character of cultured endothelial cells. Methods: Fresh operative specimens were obtained from infantile hemangiomas. The endothelial cells of IH were cultured by explant combined with trypsin-digestion technique. Immunohistochemical staining of EnVision method was carried out to identify the cultured cells. The purity of endothelial cell was examined by flow cytometry analysis of FITC-CD34. The biologic characters of endothelial cells were observed under inverted phase contrast microscope. Results: Endothelial cells were successfully cultured from 8 IH specimens of all the 14 explants. The morphology of cultured endothelial cells included two types:polygonal cells and fusifourm cells. The cultured cells were homogenously positive for EC markers-vWF or CD34, indicating their EC origin. Tube formation was found in endothelial cells of 1H. The proportion of CD34+ cells was 76.28% in cultured endothelial cells as detected by flow cytometry analysis. Conclusion: ECs can be successfully isolated and cultured from infantile hemangiomas by explant combined with trypsin-digestion technique; the cultured cells have some characters of endothelial cells.

Objective: To examine the expression of Fas and Fas ligand(FasL) in cultured infantile hemangioma endothelial cells (IHECs). Methods: IHECs were isolated and cultured by explant combined with trypsin-digested techniques. Then the expression of Fas and FasL on cultured endothelial cells (ECs) were detected by flow cytometry (FCM) and fluorescent quantitative polymerase chain reaction (FQ-PCR) 2-ΔΔCT method,and the results in ECs were compared with those in Jurkat cells (positive control) and Human umbilical vein endothelial cells(HUVECs,negative control). Results: The positive rate of Fas in cultured IHECs was (90.97±2.36)% as detected by FCM,similar to that in the Jurkat cells ([93.87±1.64]%,P>0.05) and significantly higher than that in the HUVECs (P<0.01). Fas mRNA expression in the cultured IHECs was 1.260±0.721 as detected by FQ-PCR,and was 1.448±0.059 in Jurkat cells,with their ratio being 0.87 and with on significant difference found between the 2 group(P>0.05); the ratio of IHECs to HUVECs (0.354±0.170) was 3.56 and there was significant difference between the 2 groups (P<0.01). The expression of FasL mRNA in cultured IHECs,Jurkat cells and HUVECs was too low as detected by FCM and FQ-RCR to have any biological significance. Conclusion: Fas mRNA is highly expressed in cultured IHECs and FasL is rarely expressed in IHECs, which indicates that apoptosis of IHECs is associated with high expression of Fas in IHECs.

Objective: To discuss the treatment for hemangiomas and vascular malformations. Methods: A retrospective study was conducted on 381 patients with hemangiomas and vascular malformations (January of 1998 to June of 2006). The patients consisted of 167 males and 214 females, with an age range from 1 year to 78 years (median age 16 years). According to the biological classification system introduced by Mulliken in 1982, 98 patients had hemangiomas, 38 had capillary malformations,203 had venous malformations, 27 had arteriovenous malformations and 15 had lymphatic malformations. In all patients with hemangiomas,71 patients were treated with intralesional steroids, 20 patients underwent surgical excision, 7 patients were carefully monitored by doctors. In 38 patients with capillary malformations, 27 underwent sclerosant, others were subjected to laser therapy or surgery. In 203 patients with venous malformation, 136 received sclerotherapy, 21 received surgical excision, and 11 underwent sclerotherapy conjunction plus surgical excision. Twenty-seven patients with arteriovenous malformations received sclerotherapy,interventional therapy,or sclerotherapy conjunction plus surgical excision. Fifteen patients with lymphatic malformations received sclerotherapy conjunction plus surgical excision. Results: All patients were followed-up for 0.5-6 years. Follow-up result showed that 199 patients were cured, 125 were greatly improved,and 46 were partly improved; 10 patients showed no obvious improvement; and 1 patient died. Conclusion: Correct diagnosis and the classification, along with correct treatment strategy, are the keys to increase the treatment outcome and decrease complication of patients with hemangiomas and vascular malformations.

Objective: To investigate the changes of local skin temperature in congenital arteriovenous malformations (CAVM) patients before and after treatment, and to assess the relationship between the changes of skin temperature and the outcomes of surgical treatment. Methods: From Sept. 2002 to Apr. 2006, skin temperatures around the lesions were measured in 22 CAVM patients with WMY-01 digital thermometer before and after surgical treatments, and the opposite side or adjacent skins were taken as controls. Furthermore, changes of flow signals and blood flow velocities in the lesions were measured by color Doppler ultrasonography pre- and post-operatively. Results: Skin temperatures of local lesions decreased from (35.58 ±0.68)°C pre-operatively to (34.76±0.63)°C post-operatively(P<0.01); however, the post-operative skin temperature of the lesions was still higher than that of normal skin(34.23±0.83)°C (P<0.05). Post-operatively, color Doppler flow image showed 100% occlusion of blood flow in 1 patient, more than 66.7% occlusion in 14 patients, more than 33.3% but less than 66.7% occlusion in 6 patients, and no occlusion in 1 patient; there was significant difference between those pre- and post-operation(P<0.01). The average blood flow velocity decreased from (80.86±6.97) cm/s pre-operatively to (50.72±5.85) cm/s post-operatively (P<0.01). Conclusion: A significant reduction of surface temperature in CAVMS lesions can be achieved by surgical treatment, and the temperature decrease of the lesion may be used as an indicator for the evaluation of treatment outcomes.

Adenocarcinoma ; diagnosis ; pathology ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biopsy, Needle ; methods ; Carcinoma, Squamous Cell ; diagnosis ; pathology ; False Negative Reactions ; Female ; Granuloma ; diagnosis ; pathology ; Humans ; Lung Diseases ; diagnosis ; pathology ; Lung Neoplasms ; diagnosis ; pathology ; Male ; Middle Aged ; Radiography, Interventional ; Tomography, X-Ray Computed ; Tuberculosis, Pulmonary ; diagnosis ; pathology ; Young Adult

Objective To determine the clinical value of hysteroscopy technology in high risk planned abortion. Methods A retrospective study of clinical manifestation, medical tests, treatment methods and effects of 97 cases (hospitalized from June, 2013 to October 2014) was made in the research. Of the 97 cases, 53 underwent UAE, and 44 did not undergo UAE. The risk factors and treatment features were concluded. Results The hemorrhage risk relates to menopause period. The longer menopause period, the higher the hemorrhage risk will be. The hemorrhage risk has nothing to do with HCG. For those whose menopause is less than 56 days, hemorrhage risk showed no difference between groups with or without UAE. For those whose menopause is less than 56 days, hemorrhage risk decreases in group with UAE. Hemorrhage risk and hysterectomy possibility are higher if menopause has happened for more than 11 weeks. Conclusion Cesarean scar pregnancy needs to be detected and treated early, in this way, the possibility for complications can be lowered. For cases whose menopause has been more than e weeks, hemorrhage risk should be cautioned.

Objectives To investigate the role of laminin (LM) and fibronectin (FN) in the growth cycle of human hair follicles. Methods The expression of LM and FN was detected by streptavidin-peroxidase (SP) staining. Results In the anagen phase, LM was expressed in dermal papilla, basement membrane and outer root sheath; FN was expressed in dermal papilla, basement membrane and connective tissue sheath. In the catagen phase, LM showed a lower expression in the dermal papilla and a linear expression in the basement membrane; the expression of FN in the dermal papilla and basement membrane was less intense than that in anagen phase, but was still positive. In the telogen phase, LM was only expressed in the basement membrane while FN was negative. Conclusion The difference between LM and FN expression in hair growth cycle indicates that LM and FN may play important roles in the regulation of human hair follicle growth cycl.

Objective The study was to evaluate DWI for quantifying liver fibrosis. Methods A total of 12 volunteers, 47 patients who had chronic HBV or HCV hepatitis and underwent liver biopsy [Scheuer score for fibrosis(S) and inflammation(G)] were enrolled in this study. They were scanned using a 1.5 T MR unit with b value of 0,250,500,750, 1000 s/mm~2. ADCs at b_(250-1000) and b_(500-1000) were the average ADCs of b=250, 500, 750, 1000 s/mm~2 and b=500, 750, 1000 s/mm~2. The studied the correlation between Scbeuer scores and ADC values, and conducted Mann-Whitney U test and Logistic regression to evaluate ADC for prediction of fibrosis scores. Results The average ADCs were (1.41± 0.11),(1.37±0.09), (1.27±0.05), (1.26±0.04), (1.22±0.06) mm~2/s respectively from SO to S4, stage at b=750 s/mm~2 (F=18.31, P<0.01). With the increase of fibrosis score, the average ADC decreased gradually, the two were better negatively correlated at b_(250-1000)(r=-0.727, P<0.01) than other b values. Using b_(750) and the two combined b values, the found significantly lower ADCs in S2 or greater versus S1 or less and in S3 or greater versus S2 or less fibrosis (P<0.01). The best predictor for S2 or greater was b_(750) with the largest AUC of 0.909, sensitivity of 85.7%, and specificity of 100.0% (ADC ≤1.35×10~(-3) mm~2/s). The best predictor for S3 or greater was b_(250-1000) with the largest AUC of 0.864, sensitivity of 69.6%, and specificity of 95.8% (ADC≤1.53×10~(-3) mm~2/s). Conclusion DWI can be a good predictor for scoring liver fibrosis for S2 or S3 stage above, while b_(750) and the combined b values are suitable for evaluation.