OBJECTIVE:To study female mycoplasma infection and the drug sensitivity test of mycoplasma. METHODS:Ureaplasma urealyticum(Uu)and mycoplasma hominis(Mh)of cervical secretions sampled from 5 177 female genital infection cases in our hospital were identified by cultivation,and resistance to antibiotics for them was also detected. RESULTS:Of 5 177 infection cases,mycoplasms were cultured from 2 816 specimens with total positive rate of 54.39%. Among them,there were 2 457 cases of Uu infection(47.46%),51 cases of Mh infection(0.99%)and 308 cases of mixed infection(5.95%). The highest susceptibilities of 2 816 strains of mycoplasma to antibiotic were azithromycin,josamycine and klinomycin. The highest drug resistance rates of mycoplasma were cillimycin. CONCLUSION:Rational use of drugs based on drug sensitivity test is of importance to prevent the production of drug-resistence mycoplasma.

Objective To study the effect of intravenous injection of esmolol,labetalol and their combination in the treatment of ischemic heart failure (ICD).Methods 80 patients with ICD were randomly divided into treat-ment group 1 (20 cases),treatment group 2(20 cases),treatment group 3 (20 cases)and control group(20 cases). The treatment group 1 was intravenously given esmolol on the basis of routine treatment.the treatment group 2 was intravenously given labetalol on the basis of the conventional treatment.the treatment group 3 was intravenously given esmolol and labetalol on the basis of routine treatment.The control group only received routine treatment.The thera-peutic effects of the four groups were observed and compared,and the changes of blood pressure,heart rate,walking distance and ultrasonic electrocardiogram before and after treatment were recorded.Results In the treatment group 1,3 cases with marked effect,effective in 14 cases,the total effective rate was 85%.In the treatment group 2,2 cases with marked effect,effective in 14 cases,the total effective rate was 80% cases.In the treatment group 3,4 cases with marked effect,effective in 15 cases,the total effective rate was 95%.In the control group,1 case with marked effect, effective in 10 cases,the total effective rate was 55%.The treatment effect of the treatment group 1,2,3 was better than the control group (P <0.05).The treatment effect of the treatment group 1,2 was significantly lower than the treatment group 3 (P <0.05).The treatment effect between the treatment group 1 and group 2 had no significant difference (P >0.05 ).After treatment,6 min walking distance and LVEF,FS,SV and other indicators of cardiac function were significantly improved in the four groups (P <0.05 ).The four groups had no significant adverse reactions such as nausea,vomiting,liver and renal function after treatment.Conclusion Intravenous injection of esmolol,labetalol or both drugs for treatment of ICD can improve cardiac function,with no adverse reaction,and it is safe and reliable,worthy of clinical application.

OBJECTIVE:To investigate the possible mechanism of the neuroprotective action of isatin(ISA).METHODS:A total of 40 C57BL/6J mice were divided into 4 groups:control group,model group,ISA(200 mg?kg-1 intragastrically) group,and selegiline(0.5 mg?kg-1 intraperitoneally) group.After pretreatment with drugs for 10 days,the latter 3 groups were given 1-methyl-4-phenyl-1,2,3,6-tetrahyrdropyridine(MPTP) intraperitoneally to establish Parkinson's disease(PD) model,then the mice were put to death for the determination of the activities of MAO-B,SOD and GSH-px in blood serum and brain tissue.RESULTS:As compared with blank control group,the activity of MAO-B in model group increased slightly,while the activities of SOD and GSH-px decreased(P

Currently,antimicrobial resistance has become an urgent global public health problem.Human defensin,a family of innate immune peptides,plays an important role in the control of infectious disease because of its low resistance to microbiological infection.However,so far there is seldom report on industrial production of bioactive defensin.Human β-defensin 3 (HBD3) is a member of human innate antimicrobial peptides.It has broad-spectrum antimicrobial activity and function in a variety of inflammation diseases.In addition,HBD3 is considered as a good candidate for antibiotics,because it has a strong killing activity to antibiotics-resistant microbes and resistance to highsalt environment,it is especially valuable for the treatment of ocular surface inflammatory diseases.This review covers the in vitro research on the production of HBD3,optimization of structure-activity and its antimicrobial potential for ocular surface infection,which may provide a supportive evidence for the commercial production of HBD3 and other antimicrobial peptides in biopharmaceutical area.

OBJECTIVE:To investigate observation of three metronidazol combined with drug for vaginal local application on trichomnas vaginitis. METHODS:195 patients with trichomonas vaginitis were randomly divided into three groups. They were treated with drug for vaginal local application plus single dose or multiple dose of metronidazol(p.o) ,respectively. Then their curative effects were compared. RESULTS:In single large dose of metronidazol group remission rate,cure rate and total effective rate were higher than in multiple low dose group in the first and the second day. Significant difference was noted between the two groups(P0.05) . CONCLUSION:The curative effect of single large dose of metronidazol was better than the multiple low dose. The therapeutic effect of single dose of metronidazol(1.6 g) is similar to single dose(2.0 g) .

This paper analyzed the importance of improving the humanistic quality of the students in medical university and explored the way to integrate humanistic spirit education into clinical microbiology course.

Base on the data of blood pressure, plasma lipid, Glu and UA by physical test, Support Vector Machine (SVM) was applied to identify coronary heart disease (CHD) in patients and non-CHD individuals in south China population for guide of further prevention and treatment of the disease. Firstly, the SVM classifier was built using radial basis kernel function, liner kernel function and polynomial kernel function, respectively. Secondly, the SVM penalty factor C and kernel parameter sigma were optimized by particle swarm optimization (PSO) and then employed to diagnose and predict the CHD. By comparison with those from artificial neural network with the back propagation (BP) model, linear discriminant analysis, logistic regression method and non-optimized SVM, the overall results of our calculation demonstrated that the classification performance of optimized RBF-SVM model could be superior to other classifier algorithm with higher accuracy rate, sensitivity and specificity, which were 94.51%, 92.31% and 96.67%, respectively. So, it is well concluded that SVM could be used as a valid method for assisting diagnosis of CHD.
Algorithms ; China ; Coronary Artery Disease ; diagnosis ; Discriminant Analysis ; Humans ; Models, Theoretical ; Neural Networks (Computer) ; Sensitivity and Specificity ; Software ; Support Vector Machine

0.05 ). Conclusions With low initial FGF addition of N2O does not affect the uptake of desflurane. The low-flow desflurane anesthesia is safe and economical.

Objective To investigate the pathogenesis of the susceptibility to cardiovascular diseases in patients with psoriasis. Methods The composition of serum free fatty acid(FFA) were measured by gaschromatography in 33 patients with psoriasis vulgaris and normal individuals. Results ① Along with the increase of severity of psoriasis the levels of poly unsaturated fatty acid(PUFA) increased, mono unsaturated fatty acid (MUFA) and saturated fatty acid (SFA) decreased and PUFA/SFA ratio increased. ② The level of linoleic acid (LA) was significantly higher in the patients of progressive stage and with extensive skin lesions than that in the normal group and/or stationary stage, and the level of oleic acid decreased. ③ The changes of the composition of FFA in patients with psoriasis were diffrent from the majority of the reports studied in the patients with cardiovascular disease. Discussion and Conclusion In the severe stage of psoriasis, the metabolism of fat in the liver is accelerated; the increase of LA, which supplies enough precursor for arachidonic acid metabolism, might be related to the loss of granular layer of epidermis. The changes of FFA induced by psoriasis might be a link causing cardiovascular disease but it should be studied further.

Objective] To explore a new method of synthesis of resveratrol oligmer induced by photosensitizers, further analyze its free radical scavenging activity and analgesic activity. [Method] Resveratrol was dispersed in pH 7.4 phosphate buffer solution, mixed with pheophorbide, then illuminated by laser at 630nm. The oligmers in the filtrate were analyzed by HPLC-MS and isolated by preparative liquid chromatography. Free radical scavenging activity was determined by DPPH and ABTS method, and the analgesic effect was measured by hot plate test and acetic acid writhing test in mice. [Result] A new peak occurred and reached stable in reactive solution after 20 min illumination. It was identified as the dimer of resveratrol with conversion of 35%. Its concentrations on inhibition of 50% DPPH and ABTS radical were respectively 230 μg·mL-1 and 273 μg·mL-1. It reached maximum pain threshold at 180 min, and the inhibition rate of writhing number was 62.6% with better effects than resveratrol. [Conclusion] Reseratrol can be polymerized to the dimer after pheophorbide excited by red light, the resveratrol and its dimer both have the ability of eliminating free radicals and analgesic effects, but the dimer has the stronger activity.