Objective: To explore the effect of resveratrol on the chemosensitivity of cervical cancer cells and its regulatory mechanism. Methods: Hela/DDP cells were divided into four groups: Hela/DDP group, resveratrol group, CDDP group, and resveratrol+CDDP group. Cell viability and proliferation were detected by CCK-8. Cell cycle and apoptosis were tested by flow cytometry. The expressions of p21, CDK2, cyclinD1, caspase-9, caspase-3, Bcl 2 and Bax were measured by Western blot. Results: The sensitivity of Hela/DDP cells to cisplatin was lower than that of Hela cells (P<0.01). Resveratrol significantly enhanced Hela/DDP cells' sensitivity to cisplatin (P<0.01). The inhibition of CDDP on the proliferation of Hela/DDP cells was increased by resveratrol (P<0.01). The G1 phase arrest of CDDP on Hela/DDP cells was enhanced by resveratrol (P<0.01). The expression of p21 was higher in resveratrol group and CDDP group than in Hela/DDP group, and the expressions of CDK2 and CyclinD1 were lower than in Hela/DDP group (P<0.01). Compared with CDDP group, the expression of p21 in resveratrol+CDDP group was elevated with the decrease in CDK2 and cyclinD1 expressions (P<0.01). The induction of CDDP on the apoptosis of Hela/DDP cells was elevated by resveratrol (P<0.01). The expressions of caspase-3, caspase-9 and Bax were higher in resveratrol group and CDDP group than in Hela/DDP group, and the expression of Bcl-2 was lower than in Hela/DDP group (P<0.01). Compared with CDDP group, apoptosis in resveratrol+CDDP group was increased with the reduced expression of Bcl-2 (P<0.01). Conclusion: Resveratrol enhances the chemosensitivity of cervical cancer cells by inducing cell cycle arrest and apoptosis.

Active tumor targeting drug delivery system( ATTDDS) can selectively combine with and react to the target tissue at the cellular or sub-cellular level, making it possible for drugs to be distributed in a controlled manner and released in a slow and continuous manner, subsequently resulting in enhanced anti-cancer drugs effects and reduced adverse effects on normal tissues. Although there are many problems need to be solved in research on ATTDDS, and there is still a long way to go before it can be used clinically, its roles in overcoming adverse effects of cancer treatment and improving the therapeutic effect are valuable. Generally, ATTDDS is an ideal anti-tumor drug dosage and has a promising prospect. This paper introduces the effects and characteristics of various types of ATTDDS as well as their problems and countermeasures, hoping to provide research ideas and methods for the anti-tumor targeted drug delivery.

Cardiovascular disease is a severe threat to human health and life. Among many risk factors of cardiovascular disease, genetic or gene-based ones are drawing more and more attention in recent years. Accumulated evidence has demonstrated that the loss or mutation of ataxia telangiectasia mutated (ATM) gene can result in DNA damage repair dysfunctions, telomere shortening, decreased antioxidant capacity, insulin resistance, increased lipid levels, etc., and thus can promote the occurrence of cardiovascular risk factors, such as aging, atherosclerosis and metabolic syndrome. In this review, we discusses the possible mechanisms between ATM gene and cardiovascular risk factors, which could be helpful to the related research and clinical application.
Aging ; Ataxia Telangiectasia Mutated Proteins ; genetics ; Cardiovascular Diseases ; genetics ; DNA Damage ; DNA Repair ; Humans ; Mutation ; Risk Factors

BACKGROUND:Platelet-derived growth factor has the ability of wound repair, and relevant studies mainly focus on bone tissue repair. However, there are less studies about the effect of platelet-derived growth factors in skin wound healing. OBJECTIVE:To investigate the role of platelet-derived growth factor to promote wound healing by the regulation of bone marrow mesenchymal stem cels to the wound. METHODS:Bone marrow mesenchymal stem cels from rats were cultured. Immunofluorescence method was conducted to detect cel surface markers of CD34 and CD44 in bone marrow mesenchymal stem cels. Thirty healthy male SD rats were divided into five groups at random. Bone marrow mesenchymal stem cels labeled with PKH-26 were injected into the rat caudal vein in each group. The rats were anesthetized 1 week after injection. On the center of rat back, a 3-cm incision was made to establish a wound healing model. Different concentrations of platelet-derived growth factor were injectedvia multi-points on the skin wound after modeling, and the control group was treated with the same volume of normal saline. Skin wound tissues were taken for relevant parameter measurement at 14 days after injection. RESULTS AND CONCLUSION: Under the fluorescence microscope, platelet-derived growth factor could induce the migration and accumulation of bone marrow mesenchymal stem cels to the trauma in a dose-dependent manner and promote the wound healing. Masson staining showed that, with the concentration increase, platelet-derived growth factors could reduce inflammatory cel infiltration and increase the number of colagen fibers. Results from western blot assay showed that platelet-derived growth factor could inhibit the expression of matrix metaloproteinase-1, promote the expression of tissue inhibitor of matrix metaloproteinase 1 in the wound, and inhibit the colagen degradation, thereby promoting skin wound healing indirectly.

[Objective]To establish a reproducible rat model of disc degeneration by puncturing the annulus with needles of defined gauges.[Method]Twenty skeletally mature male Sprague-Dawley rats were used to determine the depth of puncture by measuring anular thickness of lumbar disc.Twelve rats were carried out transperitoneal ventral surgery and anular stab was performed using a 21-gauge needle to make a full-thickness or partial-thickness stab incision in the L3、4,L4、5 and L5、6 anteriorlateral disc.The L6S1 level was used as a control.Magnetic resonance images scans of the stabbed discs and control discs were performed at 4,8-week time point and after MRI histological analysis were performed.[Result]The depth of puncture was respectively partial-thickness 1.5 mm and full-thickness 2.3 mm.The stabbed discs exhibited a decrease signal intensity from T2-weighted images starting at 4-week time point in full-thickness stab incision and 8-week in partial-thickness.Histological analysis revealed disc degeneration in both stab incision groups at 4-week time point and full-thickness stab incision displayed more severe degenerative changes compared with that of partial-thickness.[Conclusion]Full-thickness or partial-thickness anulus stab incision with needles of defined gauges is reliable,effective and reproducible to establish a rat model of intervertebral disc degeneration.

Objective To study a new method of kyphoplasty( Sky bone expander system) used to treat the osteoporotic compression fractures of vertebral body. Methods 74 patients with 87 vertebrae were retrospectively analyzed who had been treated with the Sky bone expander system. Based on the Genant' s grading system, 29 vertebrae were rated as Grade One, 41 Grade Two and 17 Grade Three. Both kyphoplasty and posterior internal fixation were done for 16 cases. Of all the cases, 46 were fresh fractures and 28 old. Their average VAS score was 8.7 before operation. Results The mean follow- up time was 8.6 months. All the patients reported significant pain relief. Their average VAS score was 1.4 at the third day after operation (P

Objective To investigate the current status of the indoor air pollution in urban area in Beijing and find the influence factors for the concentrations of formaldehyde and nitrogen oxides (NOx) indoors. Methods 463 persons were chosen randomly from Xicheng district of Beijing to complete the questionnaires about their indoor environment condition. The levels of formaldehyde and NOx were monitored in the bedrooms and the kitchens of 91 households. And we used the multi-regression model to find out some influence factors that related to formaldehyde and NOx concentration. Results The average daily concentration of formaldehyde in the bedrooms was about 0.049 mg/m3 and the rate of excess standard was 13%. The formaldehyde pollution in the decorated rooms (0.058 mg/m3) was more serious than that in the control rooms (0.034 mg/m3) and there was statistically significant difference between them (P

[Objective]To study the relationship between trauma and the lumbar disc degeneration and to detect the expression of the fibronectin splicing variants in the degenerated lumbar intervertebral disc,and study the expression of EDA.[Method]The human lumbar intervertebral disc tissues were collected to extract total RNA which was then amplified by RT-PCR technique.[Result]EDA~+ Fn of normal lumbar intervertebral disc were not expressed,whereas EDA~+ Fn of degenerated lumbar intervertebral disc were expressed abundantly.The defference between different groups has statistical significance.[Conclusion]EDA~+ Fn has very strongly expression in the lumbar degenerated intervertebral disc,and the trauma is one of prerequisite factors in the disc degenerated process probablely.

[Objective]To analysis the clinical manifestation and treatment of tendious sheath tubercular,probe the influencing factor of misdiagnosis and effect of treatment,in order to offer evident base for diagnosis and treatment.[Method]The material of 86 cases(from January 1994 to Jun 2005)were reviewed and statistically analyzed including sex,age,nidus region,clinical manifestation,method and effect of treatment,etc.[Result]65% of the cases were 30~49 years old.Sexual difference was not significant.Nidus region in right side was apparently higher than that in left(67%:30%).Major manifestation was edema,tenderness and mal-motion.The misdiagnosis rate and recurrent rate were 88% and 1.2%.[Conclusion]Untypical clinical symptoms and sign,non-special laboratory examinations lead to misdiagnosis.The effective treatment includes integrity excision,local and general chemotherapy,which made recurrent rate low.

0.05.[Conclusion]VDR TruⅠ and FokⅠpolymorphisms are not related to LDD in Han nationality.