[Objective]To investigate the discrepancy on biological characteristics of different subculture osteoblasts in vitro.[Methods] Compare the morphology,AKP activities and mineralization nodules of 1,3,5,7 and 9 subculture osteoblasts,and make statistic of the result.[Results] There was no discrepancy within 1,3 and 5 subculture osteoblasts,but the 7,9 subculture was significantly worse than subculture 1 in the morphology,AKP activities and mineralization nodules.[Conclusion] The osteoblasts within 5 subculture were suitable for experiment in vitro.

Objective To disclose the relationship of the target muscle function and different time interval after nerve grafting reconstructed C5 root resection in young rats.Methods Model of C5 resection was set up in 48 18-day-old SD rats.The rats were randomly divided into C5 resection group,immediate repairing group,3 days delayed repairing group,and 6,9,12,15,18 days delayed repairing groups.Each group experienced nerve grafting bridged the C5 nerve root defection at its time interval.At 6 weeks postoperatively,electrophysiological and histochemical experiment were performed.Results There was no statistical difference among the data of CMAP amplitude and latency and weight of target muscles and number of distal myelinated fiber of immediate repairing group and those of 3,6 days delayed repair group at 6 weeks postoperatively,but compared with C5 resection group,the dates was statistically higher.There was no statistical difference between the data of C5 resection group and that of 15,18 days delayed repairing group.Conclusion Nerve reconstruction for C5 root injury in young rats within 0-6 days (equal to 0-4 months in human beings) has a satisfactory protective effect on target muscles.It suggests that the OBPP children who have the operation indication should undergo surgical management in 4 months after their birth.

Cancer-related fatigue (CRF) is associated with tumor and (or) anti-tumor treatment,which has a high incidence and seriously affects the life quality of patients.The etiology of CRF involves psychology,physiology and social factors.There are various hypothesis of pathogenesis and the inflammatory system may play an important role in the generation and development of CRF.A variety of inflammatory cytokines such as IL-1β,IL-6,TNF-α,CRP are significantly correlated with CRF.

Objective Through detecting the levels of von willebrand factor (vWF) ,vWF-cleaving protease (vWF-cp)before and after percataneous coronary intervention (PCI) in patients with coronary atherosclerotic heart disease ,to evaluate the relationship between them .Methods According to the results of coronary angiography ,study objects were divided into control group (normal) and PCI group ( the diagnosis of coronary heart disease with PCI operation ) .Enzyme-linked immunosorbent assay (ELISA) was carrout to determin the levels of plasma vWF concentration and vWF-cp activity in plasma and statistical analysis .Results The pre-operative ,postoperative vWF levels in plasma in PCI group were significantly higher than that in the normal control group ;The plasma vWF levels after PCI were significantly higher than that in the group before PCI (P< 0 .05) ;vWF-cp level of the PCI group were lower than in the control group ,and postoperation is lower than preoperation ( P < 0 .05) .With the coronary artery lesion worsen ,the plasma level of vWF increased ,while the level of vWF-cp activity decreased .Conclusion vWF and vWF-cp levels with the severity of coronary atherosclerosis and thrombosis risk was increased in different decreased ,which may play an important role in the pathogenesis of acute coronary syndromes .Changes of vWF and vWF-cp level after PCI indicate that interventional therapy can increase the risk of thrombosis to some extent .

Objective To investigate the difference of the glial cell line-derived neurotrophic factor and its receptor content of proximal neurons after nerve grafting was used to reconstruct C5 root in young rats.Methods Model of C5 resection was set up in 12 18-day-old SD rats.Experimental animals were divided in to two groups, one group for C5 resection, another for nerve grafting to reconstruct the C5 defection.At 4 weeks postoperatively, the immunohistochemical staining was performed and the number of GDNF and GFRa1 immunohistochemical positive neurons were calculated respectively.Results The number of GDNF positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was 786.3 ± 176.84 and 2997.0 ±357.99, and that of C5 resection group was 335.0 ± 49.50 and 1632.0 ± 305.55.On the other hand, the number of GFRa1 positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was 787.5 ±178.55 and 3111.0 ± 445.72, that of the other group was 397.3 ± 41.78 and 1588.3 ± 229.00.The statistical analysis result showed GDNF and GFR immunohistochemical positive neurons in spinal cord and dorsal root ganglion of C5 repairing group was statistically more than that of C5 resection group(P < 0.01 ).Conclusion The neuronal protective effect of nerve grafting after reconstructing brachial plexus nerve injury in young rats may be attributed to the increase of GDNF and its receptor GFRa1 content of proximal neuron.

The use of the accessory nerve as a donor is one of the best possibilities for treatment of the brachial plexus in case of paralysis due to root avulsion. In this paper, a brief history of the use of accessory nerve tran sfer for treatment of brachial plexus injury and recent development in the anato my of accessory nerve are introduced. The nerve transfer methods and the effects of the accessory nerve are discussed in particular. The progress in and the dev elopment trend of the accessory nerve transfer methods are also analyzed. It is advisable that the accessory nerve is transferred to restore the suprascapular n erve so as to obtain shoulder abduction. And it is a clinical trend that the dis tal terminal branch of the accessory nerve is used and the function of the upper part of the trapezius muscle is preserved in the transfer. At last, the factors affecting functional outcome in accessory nerve transfer are discussed.

[Objective]To observe the complications after surgical treatment of thoracic outlet syndrome and to analyze causes and prevention of complications.[Methods]A retrospective review was done for 62 cases of thoracic outlet syndrome which had been treated by operation from January 2002 to January 2006.All the complications occrred were collected in the following time.[Results]Follow up time was 14 months to 30 months.According to assessment standard described by Wood,treatment outcome was excellent in 26 cases,good in 16 cases,fair in 11 cases and poor in 9 cases.Nine of all the 62 patients had been found complications,including hematoma in 3 cases,lymphadenectomy in 2 cases,cervical plexus injury in 2 cases,phrenic nerve injury in 1 case,brachial plexus injury in 1 case and pneumothorax in 1 case.All the patients were successfully treated.[Conclusions]The occurrences of complications are related to the skill of operation and individual differences of patients.In order to decrease and finally prevent these complications,operative experiences and preventive measures are needed.

[Objective]To investigate the pathological change of articular cartilage after impacting injury.[Method]Fifty-six New Zealand adult rabbits were divided randomly into low-energy and high-energy groups,with 28 rabbits in each group.One side of hind limb was designed as experimental side and the opposite one as control side.Four rabbits were killed at 4 days,1,2,4,8,16 weeks after impacting injury.Histological sections of the specimens were observed and stained by HE and Safranin O.the values of gray scale were obtained by imaging analysis.[Result]In low-energy group,the Mankin's score and the value of gray scale of Safranin O in experiment side was not significantly different from the control side.In high-energy group,the Mankin's score and the value of gray scale of Safranin O in experiment side increased gradually,and was significantly different from the control side.[Conclusion]In the low-energy group,the cartilage returned to the normal level at 4 weeks.In the high-energy group,the cartilage failed to be restored.The degeneration of articular cartilage was very obvious after 4 weeks.

Objective:To prepare the monoclonal antibodies against chicken CARP.Methods:The chicken CARP cDNA encoding the N-terminal 110aa length of encoding region was cloned into prokaryotic expression vector pET28b.The BALB/c mice were immunized with purified the recombinant protein,spleen cells of mouse were fused with SP2/0,and the positive clones were screened by indirect ELISA.The properties of McAb were analyzed by ELISA and Western blot.Results:Two hybridomas were proved to have the capability of stably secreting McAb against chicken CARP,designated as 4A8 and 4E6,the subtype of both was IgG1,and the light chain was ?.The titers of ascites were 3.2?104 and 1.28?105 respectively.The Western blot results suggested that they could recognize chicken CARP protein.Conclusion:Two murine hybridomas secreting anti-chicken CARP McAbs have been successfully obtained.