1.Research on evaluation methods of the Pyretic pulmonary syndrome model based on metabonomics
Jiang LU ; Changfeng LIU ; Yudi LIU ; Jinxiang ZU ; Shumin LIU
Chinese Journal of Primary Medicine and Pharmacy 2010;17(1):-
Objective To estabhsh a set of consummate evaluation methods of animal models which are with syndromes of Traditional Chinese Medicine.Methods By means of Streptococcus pneumoniae-induced pyretic pulmonary syndrome model for the entry point,in the base of the conventional evaluation methods,further attempt were made through metabonomies,through analyzing metabolic fingerprint data of rats'urine in the control group together with the model group and metabolome of rats'urine in model group at different times,in order to approach the evaluation methods of animal models.Results After rats were given Streptococctts pneumoniae through nose,compared with the control group,both the body temperature variance and lencocyte count in model group were statistically significant with P <0.01,pathological changes of lung was obvious;According to the metabolic results,metabolic profiles of rats'urine in model group changed and metabolome diverged obviously in scores plot,the research results of metabonomics were coincide with the results of macroscopy physical sign and pathological biochemistry of Pyrefic pulmonary syndrome.Conclusion Metabonomics can be used to evaluate the studies of animal models with syndromes of traditional Chinese medieine.
2.Children's hearing behavior observations and high risk individual genetic screening for late-onset hearing loss early detection and intervention exploring a basic-level hospitals model.
Yilian GUO ; Xiangli ZENG ; Ting LIU ; Yudi ZOU ; Yanchou YE
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(18):1618-1621
OBJECTIVE:
To explore the methods to detect and intervene children's late-onset hearing loss early which are suitable for basic-level hospitals.
METHOD:
Udiology and imaging diagnosis had been given to the children who passed the newborn hearing screening but showed auditory behavior disorders in the growth process, and individualized interventions were given according to the results of diagnosis. Seven children with high risk for hereditary deafness were sent to superior hospital and had molecular screening of common mutations of inherited deafness carried out, then corresponding prevention guidance and intervention were given to them.
RESULT:
Fifty-two cases with late-onset hearing loss or verbal disorders were detected by auditory behavior observations,including 4 cases of auditory neuropathy, 4 cases of unilateral sensorineural deafness, 27 cases of secretory otitis media. 13 cases of bilateral sensorineural deafness and 4 cases of autism. Seven newborns with high risk of hereditary deafness were sent to the Third Affiliated Hospital of Sun Yat-Sen University and received molecular screening of common mutations of inherited deafness. One case with GJB2 compound heterozygous mutations was detected and followed up to 4 years old, he was found bilateral moderate hearing loss and accepted the hearing aids at 2 years old. Mitochondrial DNA 1555 a > G heterogeneity mutation in 2 cases and GJB2 235 delC single heterozygous mutations in 3 cases, no mutation in 1 case, all these 6 cases have been followed-up until now, their hearing are normal.
CONCLUSION
Children's auditory behavior observations and the superior hospitals referral performing high risk individual screening for newborns with high risk for hereditary deafness can detect children's late-onset hearing loss in time, this model is suitable for basic-level hospitals.
Behavior Observation Techniques
;
Child
;
Connexin 26
;
Connexins
;
genetics
;
DNA Mutational Analysis
;
DNA, Mitochondrial
;
genetics
;
Deafness
;
diagnosis
;
genetics
;
Early Diagnosis
;
Genetic Testing
;
Hearing Tests
;
Heterozygote
;
Humans
;
Infant, Newborn
;
Male
;
Mutation
3.Use of the Y-shaped mesh in functional repair of the pelvic floor in women
Yudi ZHANG ; Dan LU ; Ping ZHENG ; Xia WU ; Hui LI ; Juhong LIU
Chinese Journal of Tissue Engineering Research 2016;20(30):4503-4508
BACKGROUND:The Y-shaped mesh graft material weaved using lightweight polypropylen has the appropriate porosity, which not only can make the vaginal tissues grow and fuse rapidly on the mesh, but also can maintain good biological strength to ensure the fixed strength for the presacral suspension. OBJECTIVE:To retrospectively analyze the clinical effect of Y-shaped mesh for the biological function reconstruction of the female pelvic floor. METHODS:Ten female cases of pelvic floor dysfunction were enrol ed, aged 37-73 years, and al were given the treatment of sacral colposuspension under laparoscopy. Then perioperative complications were recorded;patients were fol owed up regularly to record the Pelvic Organ Prolapse Quantification (POP-Q) score at different time points;and the subjective satisfaction was investigated using the Pelvic Floor Impact Questionnaire (PFIQ-7). RESULTS AND CONCLUSION:After at least 6-month fol ow-up, no postoperative pelvic infection, nerve damage and complications appeared, the patients healed wel , and no mesh erosion, infection and other adverse reactions occurred. The POP-Q and PFIQ-7 scores at 1, 3 and 6 months after repair were significantly improved than those before repair (P<0.05). These results suggest that the Y-shaped biological mesh repairing female midpelvic floor dysfunction has good biocompatibility, and can restore the anatomy of the pelvic floor.
4.Effect of uterine compression suture in the prevention and treatment of postpartum hemorrhage of cesarean section
Qiubo LI ; Weiwu LIU ; Yudi LUO ; Minlan XU
Chinese Journal of Primary Medicine and Pharmacy 2018;25(1):72-75
Objective To analyze the clinical effect of uterine artery ligation combined with modified uterine compression suture in the prevention and treatment of postpartum hemorrhage.Methods A total of 107 patients with refractory cesarean section who were treated with uterine artery ligation combined with modified uterine compression suture were selected as treatment group.Of the patients treated with conventional treatment,83 patients were included in the control group.The effective hemostasis rate,clinical curative effect,incision infection,postpartum hemorrhage,blood transfusion rate,mattress rate and postoperative morbidity were compared between the two groups.Results The amount of intraoperative blood loss (1 106 ± 458.78) mL in the treatment group was significantly higher than (753.6 ±302.30)mL in the control group,the difference was statistically significant (t =1.892,P < 0.05).The 24h total blood loss of the the treatment group [(1 028.89 ± 375.87) mL] was significantly higher than (813.87 ± 312.15) mL of the control group (t =3.872,P < 0.05).The postoperative 24h blood loss in the treatment group [(70.86 ±19.30)mL] was lower than (73.50 ± 21.20)mL in the control group,and the difference was not statistically significant (t =0.045,P > 0.05).The transfusion rate in the treatment group was 27.10%,which in the control group was higher than 10.84%,the difference was statistically significant between the two groups (x2 =7.722,P < 0.05).The effective hemostasis rate in the treatment group was 99.66% (106/107),which was significantly higher than 96.39% in the control group (x2 =6.263,P < 0.05).The rate of puerperal infection was 7.48% in the treatment group,which in the control group was 6.02%,there was no significant difference between the two groups (P > 0.05).The incision infection rate and postoperative morbidity rate were 0.00% in the treatment group and the control group,and the two groups were treated with Ⅱ / A healing.Conclusion Uterine artery ligation combined with improved uterine compression suture in the prevention and treatment of postpartum hemorrhage has good hemostasis effect,hysterectomy rate is low,it is worthy of promoting and application.
5.Clinical analysis of Blinatumomab on the treatment of refractory or relapsed precursor B-cell acute lymphoblastic leukemia
Jiao XIE ; Suxiang LIU ; Yuqiu LIU ; Yudi ZHANG ; Xitong WU ; Hailong HE ; Peifang XIAO ; Yi WANG ; Shaoyan HU ; Jun LU
Chinese Journal of Applied Clinical Pediatrics 2023;38(9):707-712
Objective:To evaluate the clinical efficacy and safety of Blinatumomab on the treatment of refractory or relapsed precursor B-cell acute lymphoblastic leukemia (R/R BCP-ALL) in children.Methods:Clinical data of children with R/R BCP-ALL treated with Blinatumomab in the Department of Hematology, Children′s Hospital of Soochow University, from August 2021 to June 2022 were retrospectively analyzed.Children were divided into<45 kg group and ≥45 kg group according to their weight at admission.They were treated with different dosages of Blinatumomab, and bone marrow remission was assessed at about 15 days.Clinical indicators and adverse events during the treatment period were recorded.The rank sum test of two independent samples were used to compare the differences between groups.The Fisher′ s test was used for comparing categorical variables. Results:Among the 16 children with R/R BCP-ALL, 12 cases (75%) achieved complete response (CR) and minimal residual lesion (MRD) turned negative at about 14 days.Among them, 5 out of 9 children with bone marrow primitive naive cell ratio≥0.5 achieved CR, and 7/7 children with bone marrow primitive naive cell ratio<0.5 achieved CR.The peak value of interleukin-6 (IL-6) in children with CR was significantly higher than those without CR ( Z=2.50, P=0.012). Twelve cases achieved CR on bone marrow assessment around day 15, and 3 cases who did not achieve CR remained in remission on day 28, with an efficacy prediction accuracy of 93.8%(15/16). Adverse events included fever, neutropenia, hypokalemia, abnormal liver function, hypocalcemia, edema, rash, hypertension, myocardial damage, abdominal pain, hypotension, and cytokine release syndrome, which were all grade 1.Neurotoxicity and death were not reported. Conclusions:The remission rate of R/R BCP-ALL in children treated with Blinatumomab was high, especially in patients with a low tumor load.The toxicity and adverse events of Blinatumomab treatment are minor and controllable.Day 15 is the optimal time point to evaluate the efficacy of Blinatumomab on children with R/R BCP-ALL, and a higher IL-6 peak can be served as a predictor of its efficacy.
6.Signal Conversion and Isolation Processing Technology Used in the Cerebrovascular Stroke Detector.
Zengshui LIU ; Yudi CHEN ; Zhaobo PEI ; Jun FENG
Chinese Journal of Medical Instrumentation 2022;46(2):141-146
By using Doppler sensor and pressure sensor, the cerebrovascular stroke detector can be used to measure the blood flow velocity and blood pressure of the carotid artery. In this study, a variety of signal conversion and isolation processing techniques are proposed for processing and feature extraction of the output signals from the sensors. Finally, effective signal output waveforms that can be used to evaluate the cerebrovascular hemodynamics index (CVHI) are obtained, and the sound signal outputs that can reflect the change characteristics of blood flow velocity and blood pressure signals are generated, which realizes the application functional requirements of the detector.
Blood Flow Velocity/physiology*
;
Cerebrovascular Circulation/physiology*
;
Hemodynamics/physiology*
;
Humans
;
Stroke
;
Technology
7. Clinical implications and prognostic value of TP53 gene mutation and deletion in patients with myelodysplastic syndromes
Huijun HUANG ; Zhongxun SHI ; Bing LI ; Tiejun QIN ; Zefeng XU ; Hongli ZHANG ; Liwei FANG ; Naibo HU ; Lijuan PAN ; Shiqiang QU ; Dan LIU ; Yanan CAI ; Yudi ZHANG ; Zhijian XIAO
Chinese Journal of Hematology 2019;40(3):215-221
Objective:
To explore the clinical implications and prognostic value of TP53 gene mutation and deletion in patients with myelodysplastic syndromes (MDS) .
Methods:
112-gene targeted sequencing and interphase fluorescence in situ hybridization (FISH) were used to detect TP53 mutation and deletion in 584 patients with newly diagnosed primary MDS who were admitted from October 2009 to December 2017. The association of TP53 mutation and deletion with several clinical features and their prognostic significance were analyzed.
Results:
Alterations in TP53 were found in 42 (7.2%) cases. Of these, 31 (5.3%) cases showed TP53 mutation only, 8 (1.4%) cases in TP53 deletion only, 3 (0.5%) cases harboring both mutation and deletion. A total of 37 mutations were detected in 34 patients, most of them (94.6%) were located in the DNA binding domain (exon5-8) , the remaining 2 were located in exon 10 and splice site respectively. Patients with TP53 alterations harbored significantly more mutations than whom without alterations (
8. A study of clinical characteristics and prognosis of primary myelofibrosis patients with thrombocytopenia in varied degrees
Zefeng XU ; Tiejun QIN ; Hongli ZHANG ; Liwei FANG ; Naibo HU ; Lijuan PAN ; Shiqiang QU ; Bing LI ; Xin YAN ; Zhongxun SHI ; Huijun HUANG ; Dan LIU ; Ya’nan CAI ; Yudi ZHANG ; Peihong ZHANG ; Zhijian XIAO
Chinese Journal of Hematology 2019;40(1):12-16
Objective:
To evaluate clinical characteristics and prognosis of primary myelofibrosis (PMF) patients with thrombocytopenia in varied degrees.
Methods:
Clinical features and survival data of 1 305 Chinese patients with PMF were retrospectively analyzed. The prognostic value of thrombocytopenia in patients with PMF was evaluated.
Results:
320 subjects (47%) presented severe thrombocytopenia (PLT<50×109/L), 198 ones (15.2%) mild thrombocytopenia [PLT (50-99)×109/L] and 787 ones (60.3%) without thrombocytopenia (PLT ≥ 100×109/L). The more severe the thrombocytopenia, the higher the proportions of HGB<100 g/L, WBC<4×109/L, circulating blasts ≥ 3%, abnormal karyotype and unfavourable cytogenetics (
9.Clinical application of critical temperature threshold measurement in cold contact urticaria
Yudi CHEN ; Bo LIU ; Xiaoting SONG ; Zuotao ZHAO
Chinese Journal of Dermatology 2020;53(5):352-355
Objective:To investigate clinical application of critical temperature threshold (CTT) measurement by using a temperature tester in the diagnosis of cold contact urticaria, and to explore relationships of CTTs with disease activity, disease control condition and quality of life in patients with cold contact urticaria.Methods:A total of 20 patients with cold contact urticaria were collected from Department of Dermatology, Peking University First Hospital from October 2017 to March 2019. Disease activity was assessed by the patients themselves at the first visit; the CTT was measured, and dermatology life quality index (DLQI) was evaluated by the patients themselves at the first visit and subsequent 2 follow-up visits. At the second follow-up visit, disease control condition was evaluated in the patients. Spearman correlation analysis was used to analyze correlations of CTTs with disease activity, CTT improvement and disease control condition. Friedman test was used to compare the improvement of CTTs and DLQI during treatment, and Bonferroni multiple test was used for multiple comparisons.Results:Based on the patients′ self-assessed disease activity, there were 3 patients with mild cold contact urticaria, 8 with moderate cold contact urticaria, and 9 with severe cold contact urticaria, and the disease activity was positively correlated with CTTs ( r s = 0.573, P = 0.008) . Clinician assessment of disease control condition showed 3 patients with poor disease control, 6 with fair disease control and 9 with good disease control, and the disease control condition was positively correlated with the improvement of CTTs ( r s = 0.516, P = 0.020) . The CTTs were (20.10 ± 4.67) ℃, (10.75 ± 5.30) ℃, and 5.50 (4.00, 10.75) ℃ (expressed as median[ P25, P75]) at the first visit and 2 follow-up visits respectively, and significantly differed among different visits ( χ2 = 34.16, P < 0.001) ; meanwhile, the DLQI scores were 19.75 ± 3.81, 8.45 ± 6.27, 0.50 (0, 9.00) (expressed as median[ P25, P75]) respectively, and there was a significant difference among different visits ( χ2 = 35.23, P < 0.001) ; the CTT and DLQI were significantly lower at the 2 follow-up visits than at the first visit (all P < 0.001) . Conclusion:CTTs can reflect disease activity in patients with cold contact urticaria, and are correlated with the quality of life and disease control condition.
10.Scoring systems for chronic spontaneous urticaria in clinical research and practice
Miao YU ; Yudi CHEN ; Bo LIU ; Xiaoting SONG ; Shuanglu LIAO ; Tingting LUAN ; Zuotao ZHAO
Chinese Journal of Dermatology 2021;54(12):1117-1121
Chronic spontaneous urticaria (CSU) greatly affects the quality of life of patients. Currently, no sensitive and convenient biomarkers are available to assess the severity of CSU and efficacy of drug therapies. It is particularly important to apply patient-reported outcome assessment tools with good reliability and validity in daily management of CSU, such as urticaria activity score, urticaria control test and chronic urticaria quality of life questionnaire. This review outlines the existing CSU assessment tools, analyzes their strengths, limitations and clinical application, aiming to establish a CSU clinical scoring system, and to facilitate personalized treatment and efficacy evaluation.