Objective To investigate the awareness of AIDS knowledge among medical staffs and their preventive behaviors among hospitals of traditional Chinese Medicine in Liangshan and to analyze the influential factors so as to provide the scientific basis for the staffs' training as well as for the prevention and control about AIDS. Methods Medical staffs from 7 hospitals of traditional Chinese medicine in Liangshan were surveyed using self-designed questionnaires,among which 276 were valid. Results The total score of the medical staffs' knowledge on AIDS was 34.14 ±6.92 points. Those with the highest score accounted for 16.73%, others with intermediate score accounted for 56.13%and still others with the low score accounted for 27.14%. The total score of the medical staffs' preventive behaviors on AIDS was 35.20 ±9.48 points, with the highest score being 12.64%, the intermediate score being 30.86%and the low score being 56.51%. The major factors to influence the medial staffs' knowledge about AIDS related to his/her educational level, specialty, education background of education, trainings on AIDS and contacts with HIV/AIDS patients and so on (<0.05) . The hospital's level, the specialty, whether or not being trained with AIDS knowledge concerned the level of occupational protection ( <0.05), whereas their ages, titles of a technical post, working years of working were irrelevant. Conclusion The overall level of these medical staffs’knowledge and preventive behaviors has not been satisfactory. Therefore, the various training programs will be necessary to improve the technical level of the medical staffs.

Objective To investigate the influence of intravenous infusion by double-lumen peripherally inserted central catheter (PICC)on continuous dynamic central venous pressure(CVP)monitoring.Methods In the patient undergoing the double-lemon PICC,continuous dynamic CVP monitoring was performed by a lumen and the intravenous infusion was conducted by another lu-men.Whether the simultaneous venous infusion affecting the values of continuous dynamic CVP monitoring was observed.Results Intravenous infusion through double-lumen PICC had no influence on simultaneously continuous dynamic CVP monitoring (P>0.05),which could influence the values of CVP monitoring when simultaneously using the intravenous infusion pump(P<0.05). The speed of pumping fluid had no influence in a shout time(10 min),with significant difference(P>0.05).The CVP values could recovered to the status before infusion when continuously infusing after stopping using the infusion pump.Compared with before in-fusion,the CVP values after stopping intravenous infusion had no obvious change(P>0.05).Conclusion Intravenous infusion by double-lumen PICC has no influence on continuous dynamic CVP monitoring.But simultaneously adding infusion pump has certain influence on the CVP monitoring values.

Objective To investigate the expression and regulation of FGFR1 protein and mRNA in human pancreatic cancer cell lines.Methods The expressions of FGFR1 protein and mRNA in pancreatic cancer cells were tested by Western blot,Northern blot and RT-PCR.The effects of exogenous growth factors and tyrosine kinase inhibitors on expression of FGFR1 protein and mRNA was observed.Results FGFR1 protein and mRNA expressed in 7 pancreatic cancer cell lines in different levels.After stimulation of several exogenous growth factors,we found that IGF-1,EGF and FGF2 up-regulated the expression of FGFR1 in Mia PaCa-2 significantly;EGF and FGF2 up-regulated the expression of FGFR1 in PANC-1 significantly (P＜0.05).The effect of FGF2 on the expression of FGFR1 was in time-dependent manner.ERK1/2 special inhibitor UO126 and p38 MAPK special inhibitor SB203580 down-regulated the expression of FGFR1.Conclusion Expression of FGFR1 is up-regulated by growth factors and may be modulated through ERK1/2 and p38 MAPK signal transduction pathway.

Intestinal mucosal mechanical barrier is one of the most important structure to maintain the body homeostasis.The occurrences of inflammatory bowel disease,necrotizing enterocolitis and poor prognosis of patients with obstructive jaundice are closely related to the damage of the mucosal barrier function.Long-term high fat diet and obstructive jaundice can cause the abnormality of bile acids metabolism.These pathological conditions are often associated with the destruction of intestinal mucosal barrier function.So the correlations between abnormal bile metabolism and intestinal mucosal mechanical barrier function have aroused interests of many researchers.They found that bile acids the important component of bile are closely related to the intestinal barrier function.The paper reviewed the recent articles and summarized the mechanisms of the deficiency of bile acids,excessive bile acids and abnormal bile acids composition damaging the intestinal mucosal barrier function.It will provide reference for the new fields of study,prevention of the toxic effects of bile acids and the improvement of the prognosis of patients.

Emergency and complications make bowel resection necessary for patients who suffer from Crohn's diseaes (CD) which is traditionally treated mainly by medical therapy. CD patients can obtain temporary relief after bowel resection. However, the high recurrence rate after surgery tends to result in repeated operations. Preoperative factors, the details of the surgery and postoperative medical treatment are considered to be related to postoperative recurrence. In this manuscript, progress on risk factors associated with postoperative recurrence in patients with Crohn's disease after bowel resection is summarized.
Colectomy ; Crohn Disease ; Humans ; Postoperative Period ; Recurrence ; Reoperation ; Risk Factors

As the third confirmed gaseous transmitters, hydrogen sulfide was found to play a vital role in the eternal milieu both physiologically and pathologically. What's intriguing is that, there exists a debate about the role of hydrogen sulfide in the pathogenesis of cancer, especially colorectal cancer. In this manuscript, progress of the effect of low and high dose hydrogen sulfide on the tumorigenesis of colorectal cancer and research on the antitumor new drugs stemmed from the function of hydrogen sulfide is summarized. Recently, the "bell-shape" model of the action of hydrogen sulfide illustrated that hydrogen sulfide was able to promote the progression of tumor in low dose and inhibit the progression of tumor in high dose. Based on these results, both hydrogen sulfide synthesis inhibitors and hydrogen sulfide donors(chimeras with NSAIDs) have been widely studied as potential anti-tumor novel drugs. According to the actual results of present studies, hydrogen sulfide synthesis inhibitors featured by aminooxyacetic acid (AOA) is more promising as a novel kind of drug. The future research based on the relationship between hydrogen sulfide and rectal cancer may bring insights into the development of novel anti-tumor drugs, thus producing tremendous potential clinical value.

Colorectal cancer is one of the leading causes of morbidity and mortality around the world. Although the staging and classification systems, such as tumor/node/metastasis (TNM) staging, are widely used in clinic, there are some limitations. For example, the patients with the same pathological type, TNM stage and treatment regimen show a completely different prognosis and outcome. In the present, molecular subtyping was concerned for a more precise and accurate staging of colorectal cancer. Herein, we reviewed the literature of the molecular subtypes of colorectal cancer in the past decades. The clinical significance of various molecular subtyping systems was evaluated and compared. It will provide reference for the precision medicine of colorectal cancer in the future.

The clinical application of novel chemotherapeutic drugs including oral 5-FU and targeted drugs and preoperatively accurate imaging grading has brought challenges to the indication criteria developed by NCCN and ESMO for neoadjuvant chemoradiotherapy in locally advanced rectal cancer (LARC). Extended hotspots have focused on the effectiveness of using capecitabine instead of fluorouracil infusion, the combination of multiple drugs and the feasibility of using neoadjuvant chemotherapy instead of neoadjuvant chemoradiotherapy for selective patients. Traditionally, the evaluation of the effect of neoadjuvant therapy has been based on the effect on the pathological complete remission (pCR) rate. However, current studies recommend the disease-free survival (DFS) as a more important outcome. Besides, seeking for effective biomarkers as predictive markers for neoadjuvant therapies or as prognostic markers remains a hotspot in the field of neoadjuvant chemoradiotherapy. The "watch and wait" approach refers to taking a close follow-up strategy instead of direct operation for patients achieving clinically complete remission (cCR) after neoadjuvant therapy. However, there is no unified evaluation criteria and time point for the evaluation of cCR following neoadjuvant therapy. Therefore, there remain a lot of controversies regarding the clinical application of neoadjuvant chemoradiotherapy in LARC. In this manuscript, research progress in the indication for neoadjuvant therapy, improvement in the neoadjuvant therapeutic schedule, advancement of the efficacy evaluation criteria of neoadjuvant therapy, the "watch and wait" approach and other hot topics is summarized to provide references for clinical practice.
Antimetabolites, Antineoplastic ; therapeutic use ; Capecitabine ; therapeutic use ; Chemoradiotherapy ; Fluorouracil ; therapeutic use ; Humans ; Neoadjuvant Therapy ; Neoplasm Staging ; Rectal Neoplasms ; therapy ; Treatment Outcome