Coronary heart disease (CHD) is a common gerontal disease, and percutaneouse coronary interangioplasty (PCI) has already become a main treatment in CHD. However, restenosis to some extend has limited clinical application of PCI. The advent of drug eluting stents brings optimistic prospect in preventing restenosis.

Objective To study the mechanism of miR-548a-3p targeting MMP-2 inhibits the metastasis and invasion of gastric cancer cells.Methods Bioinformatics were used to search those miRNAs targeting MMP-2.The level of miR-548a-3p in gastric cancer tissues were detected by qRT-PCR and the expression of MMP-2 were detected by Western blot.The level of miR-548a-3p in three reconstructed gastric cancer cell lines were detected by qRT-PCR and the expression of MMP-2 were detected by Western blot.miR-548a-3p targeting the 3'-UTR of MMP-2 were detected by Luciferase reporter assay detected.And the changes of ability of metastasis and invasion were examined by Transwell experiment before and after transfection.Results There were high expression of miR217 and low expression of MMP-2 in human poorly differentiated gastric cancer cell line BGC-823.There were low expression of miR-548a-3p and high expression of MMP-2 in human middle and high differentiated gastric cancer cell line MKN-28 and SGC-7901 (P ＜ 0.05).The result of Luciferase reporter assay showed that miR-548a-3p can targeting the 3’-UTR of MMP-2.There were high expression of miR-548a-3p and low expression of MMP-2 in MKN28-miR-548a-3p mimics.There were low expression of miR-548a-3p and high expression of MMP-2 in others reconstructed three cell lines.There were smaller invaded cells in SGC-7901-miR-217 mimics than in others three cell lines (P ＜ 0.05).Conclusions miR-548a-3p can target the 3'-UTR of MMP-2 and down regulate its expression and inhibit the ability of invasion of gastric cancer cells.

Therapeutic angiogenesis is a promising new method for the treatment of ischemic heart disease, and in recent years, implantation of autologous bone marrow cells has opened up a new pathway to such object and is now attracting many researchers' attention. This article analyzes the cell biological base of autologous bone marrow cells and the possible mechanism of neovascularization induced by implantation of autologous bone marrow cells; in addition, the effect, safety and feasibility of the implantation of autologous bone marrow cells are reviewed and commented on the basis of recent researches in animal and clinical trials.
Animals ; Bone Marrow Cells ; cytology ; Bone Marrow Transplantation ; Humans ; Myocardial Ischemia ; therapy ; Neovascularization, Physiologic

Aim To study the influence of estradiol on peripheral blood stem cells and angiogenesis in a rat model of myocardial infarction.Methods Acute myocardial infarction(AMI)model was bulit by ligation of left anterior descending coronary in ovariectomized SD rats.Then,they were treated with different doses of estradiol benzoate.CD34+ cells in myocardium at 24 h after AMI were detected by immunohistochemistry,and CD34+ cells in peripheral blood were measured by flow cytometry on day 1,3 and 7 after AMI.The expression of stromal cell-derived factor-1(SDF-1)in myocardium on day 1,3 and 7 after AMI and capillary density in myocardium at 28 days after AMI were evaluated by immunohistochemistry.Results CD34+ cells in peripheral blood and myocardium,the expression of SDF-1 and capillary density at 4 weeks after AMI in ovariectomized rats were obviously lower than those in ovary intact rats.However,after indication with different doses of estrogen,CD34+ cells in peripheral blood and myocardium,the expression of SDF-1 and microvascular density were increased in ovariectomized rats with AMI,this effect was strongest especially in estrogen replacement therapy group.Conclusions Estrogen could improve the mobilization and homing of bone marrow stem cells into myocardium,increase capillary density and improve prognosis after AMI,and this mechanism could be mediated by promotion of expression of SDF-1 in myocardium.Moreover,the effect of estrogen replacement before AMI was better than estrogen therapy after AMI.

Emodin has such pharmacological effects as ant-inflammatory, anti-tumor, immunoregulation. Meanwhile, emodin could be used for inhibiting the proliferation of vascular smooth muscle cells (VSMC). Many foreign studies demonstrated that emodin had an effect on inhibiting proliferation of VSMCs and cell migration and promoting cell apoptosis, and probed into molecular mechanisms in all aspects. Besides, clinical translational researches and application explorations were also carried out. This article summarizes the research progress of the anti-proliferation effect of emodin on VSMCs.
Apoptosis ; drug effects ; Cell Movement ; drug effects ; Cell Proliferation ; drug effects ; Emodin ; pharmacology ; Humans ; Muscle, Smooth, Vascular ; cytology ; drug effects ; metabolism ; Signal Transduction ; drug effects

OBJECTIVETo investigate the association of single nucleotide polymorphism (SNP) rs599839 on chromosome 1p13.3 with premature coronary heart disease.

METHODSA case-control association study of 303 unrelated premature coronary heart disease patients and 312 normal controls from a Chinese Han population was performed. Genotype was determined by polymerase chain reaction-restriction fragment length polymorphism for SNP rs599839 on Chromosome 1p13.3.

RESULTSThe frequencies of the G allele were 5.0% and 9.1% in the premature coronary heart disease group and control group respectively (P= 0.004). The presence of the G allele was associated with significantly lower concentration of the low density lipoprotein-cholesterol (LDL-C) in both groups.

CONCLUSIONThe present findings suggest that the genetic polymorphism in rs599839 may be associated with the development of premature coronary heart disease in Chinese Han population, and the polymorphism may have some influence on serum LDL-C level in this population.

Adult ; Aged ; Asian Continental Ancestry Group ; genetics ; Case-Control Studies ; Chromosomes, Human, Pair 1 ; genetics ; Coronary Disease ; blood ; genetics ; pathology ; Ethnic Groups ; genetics ; Female ; Gene Frequency ; Genotype ; Humans ; Lipids ; blood ; Male ; Middle Aged ; Polymorphism, Single Nucleotide

Tanshinone IIA nanoparticles were constructed and perfused in rabbit's right carotid after intimal denudation with ballon. Localization and retention at different time points of the coumarin-labeled drug nanoparticles were evaluated under laser confocal microscope. Nanoparticles were seen in the three layers of the cross-section artery. At 7 days, they were mainly deposited in the medial layer, while the deposition was generally observed in the adventitia and media at 14 days and 28 days. In the Tanshinone IIA nanoparticle study, a significant reduction of the neo-intimal hyperplasia was noted by comparing the intimal area and the intima-media ratio in the three groups. And the PLGA nanoparticles appeared to be fully biocompatible. As a result, the local administration of the nanoparticles with incorporated Tanshinone IIA showed not only the preventive effects, but aslo the high absorption and good biocompatability in the whole arterial wall.
Angioplasty, Balloon ; adverse effects ; Animals ; Caprolactam ; administration & dosage ; analogs & derivatives ; Carotid Arteries ; pathology ; Diterpenes, Abietane ; Drug Carriers ; Hyperplasia ; etiology ; prevention & control ; Lactic Acid ; administration & dosage ; Nanoparticles ; Phenanthrenes ; administration & dosage ; pharmacology ; Polyesters ; Polymers ; administration & dosage ; Rabbits ; Tunica Intima ; drug effects ; pathology

Purpose: Intrahepatic cholangiocarcinoma (ICC) is one of the most common liver primary tumors but its treatments are limited. Bioinformatics showed that the expression level of long non-coding RNA cancer-associated susceptibility 15 gene (CASC15) is correlated with ICC progression, but its functional mechanism remains unclear. Materials and Methods: Tissues from ICC patients, tumor and adjacent tissue, were used for detection of the expression of CASC15. Clinical data were also collected for clinicopathologic and survival analysis. Short interfering RNA and lentiviral short hairpin RNA were used to knock down CASC15 and PRDX2 expression in ICC cell lines, for the analysis of changes of cell function and xenografts. RNA-pulldown and RNA immunoprecipitation assays were used to detect RNA-binding protein, PRDX2. Male nude mice were used for ICC xenografts, and livers were collected after 4 weeks for immunohistochemistry. Results: CASC15 is highly expressed in ICC tissues and is related to higher TNM stage. Knockdown of CASC15 in ICC cells reduced cell proliferation, migration, invasiveness and increased apoptosis, and G1/S block. PRDX2 bound to CASC15. Knockdown of CASC15 decreased PRDX2 expression which was rescued by the inhibition of proteasome formation. Downregulation of PRDX2 resulted in G1/S block, reduced ICC cell invasion. Downregulation of CASC15 inhibited phosphoinositide 3-kinase (PI3K)/AKT/c-Myc pathway through downregulating of PRDX2 and overexpressed PRDX2 rescued the block. CASC15 knockout in ICC xenografts suppressed tumor development in vivo, decreased the expression of PRDX2 and Ki67 and inhibited PI3K/AKT pathway. Conclusion CASC15 promotes ICC possibly by targeting PRDX2 via the PI3K/AKT pathway, indicating poor prognosis and high degree of malignancy of ICC.

Objective To examine survival differences in prognosis and survival between patients with HPV-positive and those with HPV-negative oropharyngeal squamous cell carcinoma (OPSCC).Methods Pubmed,Embase,Web of science and Medline databases were searched from their inception till June 2014.A random-effect meta-analysis was used to pool study estimates evaluating overall (all-cause mortality),disease-specific (death from OPSCC),disease-free (recurrence free),progression-free survival outcomes and local regional control rate in HPV-positive vs HPV-negative OPSCC.After study selection,two reviewers assessed and extracted data independently.Meta-analysis was performed using the STATA 11.0software.Results Thirty-seven studies were included.HPV-positive OPSCC patients had a better overall survival compared to HPV-negative patients (HR 0.39,95％ CI 0.32-0.46).HPV-positive OPSCC patients had a significantly lower disease specific mortality (HR 0.31,95％ CI 0.22-0.39) and were less likely to experience progression or recurrence of their cancers than HPV-negative patients (HR 0.34,95％CI 0.25-0.42).Both disease-free survival and progression-free survival were significantly improved in patients with HPV-positive OPSCC (HR 0.38,95％ CI 0.29-0.47 and HR 0.46,95％ CI 0.29-0.63,respectively).Conclusions HPV infection is an important prognostic factor of OPSCC.Stratified therapies can be applied in OPSCC based on HPV status of tumours.

Objective To investigate the mechanism of Zuogui Pills (ZGP) on improving the stemness of ovarian germline stem cells (OSCs) in ovarian aging rats through the gap junction protein connexin 43 (Cx43) phosphorylation based on the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) pathway. Methods Eight-week-old female SD rats were randomly divided into control(CON),model(cyclophosphamide,CTX),ZGP and dehydroepiandrosterone(DHEA) groups,six rats in each group. CTX was injected intraperitoneally for 15 days to establish a model for ovarian aging,and then ZGP (1.85 g·kg-1) or DHEA (8.1 mg·kg-1) was administered by gavage once daily for 30 days. Follicle count at different stages and pathological changes of ovarian tissues were observed by HE staining. ELISA was used to detect serum levels of anti-mullerian hormone (AMH),follicle-stimulating hormone (FSH),and estradiol(E2). The expression levels of related proteins (PCNA,MVH,Oct4,Cx43,p-Cx43,EGFR,AKT、p-AKT) in ovarian tissue were detected by Western Blot. Results Compared with the CON group,rats in CTX group had significantly reduced body mass(P<0.05) and ovarian mass(P<0.05). The number of primordial follicles decreased significantly(P<0.01). Serum FSH level increased remarkably(P<0.01),while AMH and E2 levels showed a downward trend (P>0.05). The protein expressions of Oct4,MVH,PCNA,EGFR,p-AKT/AKT,p-Cx43(Ser368)/Cx43 and Cx43 in ovarian tissue were obviously decreased(P<0.05,P<0.01). Compared with the model group,the body mass of rats in ZGP group was significantly increased (P<0.05),as well as ovarian mass and ovarian coefficient were remarkably increased (P<0.01). The number of primordial follicles were increased significantly (P<0.01),and E2 level increased significantly (P<0.05). The protein expressions of Oct4,MVH,PCNA,EGFR,p-AKT/AKT,p-Cx43(Ser368)/Cx43 and Cx43 in ovarian tissue were all increased significantly (P<0.05,P<0.01). Conclusion ZGP may ameliorate ovarian aging by maintaining the stemness of OSCs. Its mechanism of actions is related to the PI3K/AKT signaling pathway and gap junction protein phosphorylation.