Objective To investigate the antiproliferative effect of suramin on trabeculectomy in rabit.Design Experimental study. Participants 32 New Zealand albino rabbits.Methods 32 albino rabbits were randomly divided into four groups.Both eyes of rabbits were performed trabaculectomy and placed a piece of cotton with 0.3 mg/ml mitomycin C(MMC)for 2 minutes in standard control group,220 mg/ml suramin for 2 minutes in experimental groupⅠ,250 mg/ml suramin for 2 minutes in experimental groupⅡ.The examination of intraocular pressure was performed before surgery.The filtration bleb and intraocular pressure were observed at 3,7,15, 30 days after surgery.Some conjunctiral specimen was observed with histochemicall(HE staining)and immunohistochemicall(PCNA staining)methods.Main Outcome Measures Intraocular pressure(IOP),filtering bleb appearance,histopathological staining.Results The percentage of the functional blebs in empty control group and experimental groupⅠwas lower than in standard control group,and experimental groupⅡwas higher than in the other 3 groups at 30 days after surgery.Immunohistochemical staining showed the number difference of PCNA positive cell nuclear was no significant between experiment groupⅠand standard control group,but less than empty control group,and experiment groupⅡwas the least(P

Objective To evalute Qiliqiangxin capsule for improving cardiac function and identifiy Qiliqiangxin capsule for directing cytoprotective effects against apoptosis of cardiomyocytes. Methods Sprague Dawley (SD) rats were tested as subjects and the model rats were built by anterior descending coronary deligation, then the survival rats were devided into 6 groups randomly:sham-operated group(sham,n=8),fed with normal saline of same volume instead;control group(control,n=7),fed with normal saline of same volume instead;Qiliqiangxin great dosage group(great,n=9),Qiliqiangxin 1.2g/(kg?d) treatment;Qiliqiangxin medium dosage group(medium,n=9),Qiliqiangxin 0.6g/(kg?d) treatment;Qiliqiangxin small dosage group(small,n=8 rats),Qiliqiangxin 0.3g/(kg?d) treatment;the captopril group(captopril,n=9),captopril 25mg/(kg?d) treatment.24 hours later,rats in sham operated group and model group were given normal saline,rats in treating group were given Qiliqiangxin,rats in the contrasting group were given captopril.After being treated for 4 weeks, all rats′ heart function was measruated by right flank arteria carotis communis arterial cannula.The left ventricle mass index was calculated and the expression of protein caspase-3 was tested by immunohistochemical method and Westernblot. Results Compared with control group, Qiliqiangxin capsule could reduce effectively BW,HMI,LVMI,increase LVEDP and LVSP,+dp/dtmax and -dp/dtmax.Immunohistochemical analysis and Westernblot analysis showed that adminstration of Qiliqiangxin significantly inhibited caspase-3 expressions in myocardial cells in a dose-dependent manner.Conclusion Qiliqiangxin significantly improved the CHF rat′s cardiac function and reduced the left ventricle mass index and heart mass index 4 weeks after being treated with relative high dose Qiliqiangxin. It might be partially attributed to the suppression of caspase-3 expressions.

Objective To develop a machine simultaneously integrating mechanized environmental cleaning and automatic mollusciciding and to evaluate its effectiveness of field application,so as to provide a novel Oncomelania hupensis snail control technique in the large?scale marshlands. Methods The machine simultaneously integrating mechanized environmental clean?ing and automatic mollusciciding,which was suitable for use in complex marshland areas,was developed according to the mech?anization and automation principles,and was used for O. hupensis snail control in the marshland. The effect of the machine on environmental cleaning and plough was evaluated,and the distribution of living snails was observed at various soil layers follow? ing plough. The snail control effects of plough alone and plough followed by mollusciciding were compared. Results The ma?chine could simultaneously complete the procedures of getting vegetation down and cut vegetation into pieces,plough and snail control by spraying niclosamide. After plough,the constituent ratios of living snails were 36.31% ,25.60% ,22.62% and 15.48% in the soil layers at depths of 0-5,6-10,11-15 cm and 16-20 cm respectively,and 61.91% living snails were found in the 0-10 cm soil layers. Seven and fifteen days after the experiment,the mortality rates of snails were 9.38% and 8.29% in the plough alone group,and 63.04% and 80.70% in the plough + mollusciciding group respectively(c27 d = 42.74,c215 d =155.56,both P values < 0.01). Thirty days after the experiment,the densities of snails were 3.02 snails/0.1 m2 and 0.53 snails/0.1 m2 in the soil surface of the plough alone group and the plough + mollusciciding group,which decreased by 64.92% and 93.60% ,respectively,and the decrease rate of snail density was approximately 30% higher in the plough + mollusciciding group than that in the plough alone group. Conclusions The machine simultaneously integrating mechanized environmental cleaning and automatic mollusciciding achieves the integration of mechanical environmental cleaning and automatic niclosamide spraying in the complex marshland areas,which provides a novel technique of field snail control in the large?scale setting in Chi?na.