The recent researches of air pollution and human health in Liaoning province were summarized in the paper and some considerations for the future study on this subject were put forward, such as the technique of health-based risk assessment in special area, the model of characteristic air pollutants dose-response assessment, the does-response relationship.

Objective:To evaluate the possibility of prevention of Frey's syndrome by reservation and reconstruction of parotid fascia in parotidectomy.Methods:Parotidectomy with parotid fascia reservation and reconstruction was conducted in 31 cases. 12 months-12 years after operation the patients were examined by Minor test.Result:Positive reaction of Minor test was observed in 3 out of the 31 cases(9.67%).Conclusion:A parotidectomy with reservation and reconstruction of parotid fascia can efficiently prevent Frey's syndrome.

Objective:To evaluate fixation effect of intermaxillary fixation screw.Methods:63 cases with mandibular fractures were treated with open reduction,and then internal fixation were managed with intermaxillary fixation using dental arch splint or intermaxillary fixation screw randomly.The operation time,postoperation periodontal index,mouth open degree,occluding relation and fracture healing were compared.Results:There was no significant difference upon occluding relation,fracture healing and mouth open degree under two treatment methods.Compared with dental arch splint fixation method,intermaxillary fixation screw had shorter operation time and better periodontal index.Conclusion:Intermaxillary fixation screw is a good intermaxillary fixation method because of short operation time.Furthermore,it can provide enough traction retention strength and easy to maintain oral hygiene.

AIM:To evaluate the influence of scutellarin on the expression of vascular endothelial growth factor ( VEGF) in high glucose-treated human retinal pigment epithelial cell line ARPE-19 and to observe the effects of scutellarin on the protein expression of VEGF, p-ERK and VEGFR2 in the retinas of type II diabetic rats.METHODS: Cultured ARPE-19 cells were divided into normal control group, scutellarin group, high glucose group and high glucose+scutellarin group.The protein levels of VEGF, p-ERK and VEGFR2 were measured by Western blot.The VEGF release in ARPE-19 cells was detected by ELISA.Normal rats were randomly divided into normal control group and scutellarin group.Diabetic rat model was established by feeding with high-fat diet and injecting with streptozocin, and randomly divided into diabetes group and diabetes treated with scutellarin group.After 16 weeks, the eyes were removed.The morphological changes of the retinas were observed under light microscope with HE staining, and histopathological score was recorded.The expres-sion of VEGF in the retinas was observed by the method of immunohistochemistry.RESULTS:Compared with normal con-trol group, the protein levels of VEGF, p-ERK and VEGFR2 in the ARPE-19 cells decreased in scutellarin group, but in-creased in high glucose group.The histopathological score of the retinas showed significant difference among diabetes group, diabetes treated with scutellarin group and normal control group, and no significant difference between normal con-trol group and scutellarin group was observed.The expression of VEGF increased in diabetic group and was significantly higher than that in scutellarin treatment group (P<0.05).CONCLUSION:Scutellarin inhibits the increased protein le-vels of VEGF, p-ERK and VEGFR2 in ARPE-19 cells, and decreases the expression of VEGF in the retinas of diabetic rats.The suppression of the diabetic retinopathy development by scutellarin may be partly involved in the ERK/MAPK pathway.

This paper reports the effects of the polysaccharide from Sargassum fusiforme (SFPS) on contents of lipoid peroxide (LPO) and enzyme activities of glutathione reductase (GR), glutathione peroxidase (GSH - PX), catalase (CAT), superoxide dismutase (SOD) in whole blood, liver and spleen in leukemia L615 mice. The results show that SFPS remarkably reduces the contents of LPO and increases in the enzyme activities of CAT , SOD in whole blood, liver and spleen in leukemia L615 mice. The authors point out that SFPS may remove the free radicals in the body of leukemia L616 mice and have an effect against lipid peroxidation. This may be one of the mechanisms of SFPS against leukemia.

In this paper,the effects of SFPS on red blood cell immune function of P388mice is presented. The results suggest that SFPS' enhancing the immune function of red blood cells of P388 mice may be related to decreasing the content of LPO of red blood cell membranes inhibiting the formation of HMP by protein of red blood cell membranes and systolic protein,increasing the sealing degree of red bold cell menbranes and the content of sialic acid and enhancing the activities of SOD, CAT, and Na+, K+-ATPase of red blood cell membranes.

Objective To study and observe the clinical efficacy of using non skid plate by posteromedial approach for fixing splitting or compression tibial plateau fracture.Methods Twenty eight cases of patients who were treated for fixing splitting or compression tibial plateau fracture from January 2014 to January 2016 in people's hospital of Xinyu were retrospectively reviewed.All patients were treated byposterior medial approach with non skid plate for fixation,all patients were followed up by postopera tive imaging and clinical follow-up to observe its clinical curative efficacy.Results Twenty-eight patients were followed up,the mean follow-up time was 12 months.All patients had no internal fixation loosening fracture,send,vascular and nerve damage and other adverse reactions.All the patients were healed within 4 to 7 months,the average healing time was 18.5 weeks.After healing,knee joint function was evaluated,18 cases were excellent,5 cases were good,the excellent and good rate was 82.1 ％.Conclusion The efficacy of using non skid plate by posteromedial approach for fixing splitting or compression tibial plateau fracture is good,it is worthy of clinical application.

Objective To evaluate whether it can prevent Frey's syndrome by reserving parotid fascia or reconstructing parotid fascia with sternocleidomastoid muscle and remaining of parotid fascia in parotidectomy. Methods Fifty-two patients undergoing parotidectomy with maintained and reconstructed parotid fascia were followed up. A test of chewing vitamin C slice guidance and iodine-amylum pilot was conducted for observing the incidence of postoperative Frey's syndrome. Results Flush with perspires was found only in 5 out of the 52 patients (9.61%), which was lower than the incidence after traditional parotidectomy. Conclusion The method of reserving and reconstructing parotid fascia with sternocleidomastoid muscle and remaining of parotid fascia in parotidectomy can efficiently prevent Frey's syndrome, and can also repair the hollow abnormity of the face.

BACKGROUND:Hyperbranched cationic amylopectin is a kind of nonviral gene vectors with low toxicity and good transfection efficiency. However, searching for more efficient methods to delivery it into the body and making the genes expressed are being explored.
OBJECTIVE:To study the expression of DMAPA-Amp wrapped green fluorescent protein (GFP) transferred by modified carotid injection into cerebral ischemic area.
METHODS:Male Sprague-Dawley rats subjected to middle cerebral artery infarction were randomly divided into two groups after 24 hours:experimental group (injected with GFP entrapped DMAPA-Amp via the internal carotid artery) and control group (injected with GFP entrapped DMAPA-Amp via the tail vein). These rats were put to death and their brain tissue was removed after 7 days. The expression of GFP was detected by quantitative PCR and western blot assay, and immunofluorescence staining was performed to detect the expression of GFP located near cerebrovascular endothelial cel s by frozen section.
RESULTS AND CONCLUSION:Compared with the control group, the expression of GFP was much higher in the experimental group detected by quantitative PCR and western blot (P< 0.05). Additional y, the expression of GFP located near cerebrovascular endothelial cel s by frozen section was also higher than that in the control group. Modified carotid injection could significantly promote the expression of hyperbranched cationic amylopectin derivates and GFP in the brain tissue of Sprague-Dawley rats undergoing middle cerebral artery infarction compared with tail vein injection, which indicates DMAPA-Amp and modified carotid injection may cast new lights on the therapy for angiogenesis of ischemic stroke.

BACKGROUND:Human has a high level heritability in physical performance. With the development of technology and test method in molecular biology, the researchers of sport science are concerned with the influence of gene variation on the elite athlete performance. They begin to know the important value of gene on predicting the physical performance.
OBJECTIVE:To review the research results in the field of gene polymorphisms and elite athlete performance and to expatiate the problems in these researches, thereby offering some proposals.
METHODS:A computer-based online research of PubMed and CNKI databases was performed to col ect articles published from 1998 to 2013 with the key words“elite athlete performance, gene polymorphisms, endurance, power, training response”in Chinese and English. There were 150 articles after the initial survey. A total of 80 articles were included according inclusion and exclusion criteria.
RESULTS AND CONCLUSION:The researches of this field are mainly focused on the three aspects:elite endurance performance, elite power performance, and training response, which are associated with gene polymorphisms. The main genes related to elite endurance performance are ACE, mtDNA, PPAR, ADR, GNB3, NRF2, etc. The main genes related to elite power performance are ACTN3, ACE, GDF-8, IL-6, HIF-1, etc. The main genes related to training response are HBB, TFAM, NRF2, AR, FECH, etc. Several gaps in the current researches have been identified including smal sample size of most athletic cohorts, lack of corroboration with replication cohorts of different ethnic backgrounds. The numerous research findings can be applied to the gene selection of athletes by creating some kinds of algorithms and models.