Bcl-2 family have dual-regulating effects on cell apoptosis mediated by mitoehondrion. The ratio of pro-apoptosis members and anti-apoptosis members closely correlates with tumorigenesis, drug-resist-ance and prognosis. Therefore,Bcl-2 family become important targets in tumor biotherapy. Many strategies have been applied to tumors treatment targeting Bcl-2 family,such as some biological treatment,short peptides and organic small molecules.

Objective To establish a method for the content determination of paeoniflorin in Gujing Pill. Methods Orthogonal test was used to optimize the condition of sample extraction and paeoniflorin content was detected by HPLC. HPLC was performed with Merck- LiChrospher RP- C18 column(4.6? 250 mm,5 ? m); methanol- water (25 ∶ 75) served as mobile phase and the detection wavelength was at 230 nm, the flow rate being 0.8 mL/min. Results Paeoniflorin has a good linearity within 0.27～ 1.35? g(r=0.9998) and the average recovery was 97.08 % with RSD 2.02 % (n=10). Conclusion This method is simple, sensitive, specific and can be used for the quality control of Gujing Pill.

Objective: To explore the clinical effect of modified preflush on the treatment of CRRT without heparin in patients received dialysis with complication of cerebral hemorrhage. Methods: 44 patients received dialysis with complication of cerebral hemorrhage were divided into control group (28 cases) and observation group (16 cases) according to the implemented time of modified preflush. A retrospective analysis was adopted to compare the effect of filter in blood coagulation grade and service life between conventional preflush (control group)and modified preflush (observation group). Results: The differences of I, II, III grade of blood coagulation between the two methods were significant (x2=12.020, P<0.05), and the differences of average service time of filter, transmembrane pressure(TMP), pressure of filter and value of pressure drawdown (ΔP) between the two methods also were significant (t=3.535, t=4.024, t=6.071, t=3.524, P<0.01). Besides, the difference of venous pressure (PV) between them was significant (t=2.166, P<0.05), while the differences of BFR, UFR and Pa between the two methods were not significant (t=0.263, t=1.162, t=0.258, P>0.05). Conclusion: Modified preflush method can make the fiber of filter to obtain more full stretch and soak, and it also can prolong the service of time, and decrease incidence of blood coagulation, and ensure curative effect and smooth process.

Objective:To investigate the clinical pharmaceutical care methods and promote rational drug use. Methods:The diag-nosis and treatment process of one patient with chronic obstructive pulmonary disease were used as the examples, the pharmacists par-ticipated in selecting drugs, adjusting dosage and making individual regimen to carry out the pharmaceutical care in the whole treatment process, including observing curative effect, monitoring adverse reactions, implementing patient education,nutritional support, immu-notherapy and the effect evaluation. Results:Clinical pharmacists together with clinicians developed the treatment regimens for the pa-tient with the full implementation of pharmaceutical care, the effect was promising, and the patient recovered and discharged from hos-pital. Conclusion:Clinical pharmacists should implement pharmaceutical care for patients with chronic obstructive pulmonary disease in the whole treatment process, which can reduce drug adverse reactions and interactions effectively, and play an important role in ra-tional and safe drug use.

ObjectiveTo compare the therapeutic efficacy of acupuncture at Diji (SP8) versus acupuncture at Dijiand Guanyuan (CV4) in treating primary dysmenorrhea, for exploring the combination relation of the commonly-used acupoints in acupuncture treatment for primary dysmenorrhea.MethodForty patients with primary dysmenorrhea were randomized into a single acupoint group and a double acupoint group, 20 in each group. The single acupoint group was intervened by acupuncture at Diji, while the double acupoint group was by acupuncture at Diji and Guanyuan. The change of Visual Analogue Scale (VAS) score was observed and compared between the two groups.ResultThe changes of VAS score in the double acupoint group were more significant than that in the single acupoint group at each different time point.ConclusionAcupuncture at Diji alone and acupuncture at Diji and Guanyuan both can effectively treat primary dysmenorrhea; however, acupuncture at Diji and Guanyuan tends to produce a more significant effect than acupuncture at Diji alone; Diji and Guanyuan may work synergistically in acupuncture treatment for primary dysmenorrhea.

Objective To observe the sleeping time and analyze its impact on the psychological state and quality of life of patients with type 2 diabetes .Methods Convenience sampling was used to recruit 365 patients with type 2 diabetes in Jiangsu Province Hospital on Integration of Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine between April 2014 and April 2015 .All the patients were divided into two groups according to the six-hour cut-off point of sleeping time .They were investigated by means of World Health Organization Quality of Life-BREF ( WHOQOL-BREF), Diabetes Distress Scale ( DDS) and General Self-Efficacy Scale ( GSES).Data were analyzed by t-test, chi-square test, Mann-Whitney U test and multivariate regression analysis .Results The average sleeping time was 7.03 h, and the sleeping time of 109 patients was less than 6 h.Patients with less sleeping time had lower quality of life and self-efficacy scores as well as higher diabetes distress scores than those with sleeping time more than 6 h.The differences were statistically significant (P<0.05).Regression analysis showed that sleeping time was the factor of quality of life (β=0.117, P=0.047), self-efficacy (β=0.136, P=0.024) and diabetes distress(β=-0.118, P=0.046).Conclusion Sleeping time affects the psychological state and quality of life of patients with type 2 diabetes.The medical should pay more attention to the quality of sleep of such patients .

Objective To construct the lentiviral vector encoding CLEC4M and prepare K -562 cells with stable overexpression of CLEC4M.Methods The gene sequence of normal CLEC4Mwas cloned by reverse transcription PCR and then inserted into GV166 vector to construct GV166-CLEC4Mlentiviral expression vector,and then lentiviral packaging was performed by transfection of293T cells.The ob-tained lentiviral liquid was used to infect human leukemia cell line K-562.Real-time PCR and Western blot were used to detect the over-expression of CLEC4M in K-562 cells.Results Sequencing showed that the recombinant lentiviral expression plasmid GV166-CLEC4M was successfully constructed.Lentiviruses could efficiently infect K-562 cells,according to real-time PCR.CLEC4Mwas successfully o-ver-expressed in K-562 cells at mRNA and protein levels.Conclusion The construction of lentiviral vector encoding CLEC4M lays a foundation for further study of CLEC4M gene involved in HCV entry into host cells.

Objective To explore the early diagnostic values of serum alpha -fetoprotein (AFP),gamma -glutamyltransferase Ⅱ (GGTⅡ),and Golgi protein 73 (GP73)in patients with primary hepatocellular carcinoma (PHC).Methods The serum specimens of 100 pa-tients with liver diseases (50 cases of hepatitis and liver cirrhosis and 50 cases of PHC)and 50 healthy people were collected in our hospital from February 2013 to February 2014.Electrochemical luminescence technique,specific immuno -membrane adsorption assay,and enzyme-linked immunosorbent assay were used to measure the serum levels of AFP,GGTⅡ,and GP73.Comparison of continuous data between multiple groups was made by analysis of variance,and comparison between two groups was made by q test.The receiver operating character-istic (ROC)curves of single or combined test results were made,and the areas under the ROC curves (AUCs)were calculated.The sensi-tivity,specificity,and AUCs of AFP,GGTⅡ,GP73,and the combined test were analyzed and compared.Results The level of serum GGTⅡ in the PHC group was significantly different compared with those in the other two groups (F =16.224,P <0.05),but there was no significant difference between the normal group and the hepatitis and liver cirrhosis group (P >0.05).Significant differences in serum levels of AFP and GP73 were observed by paired comparison between the PHC group,hepatitis and liver cirrhosis group,and normal group (F =193.128,F =20.231,P <0.05 for both).When assayed alone,the specificities of GP73,GGTⅡ,and AFP were 69%,64% and 51%, respectively,and the sensitivities were 92%,84%,and 76%,respectively.In combined test,the specificity was 94.6% and the sensitivi-ty was 98.8%.Conclusion The GP73 test is the best performer in the single assays.Combined test of serum AFP,GGTⅡ,and GP73 shows a good diagnostic value for PHC with greatly improved specificity and sensitivity.

Objective To explore the antagonistic effect of Ziyin Xiehuo Recipe(ZYXHR,Recipe for nourishing kidney yin and purging ministerial fire) on estrogen-like activity of environmental endocrine disruptors,nonylphenol(NP) and mixture of NP and bisphenol A(BPA). Methods Totally 30 three-week-old female SD rats were randomly divided into control group(fed with corn oil),exposed group A (feeding NP100 mg / kg ),exposed group B (feeding NP 50 mg/kg+ BPA 200 mg/kg ),treatment group A (NP 100 mg/kg + ZYXHR),treatment group B (NP 50 mg/kg+ BPA 200 mg/kg + ZYXHR). After 15 days of treatment,the uterine weight gain and PCNA protein expression were detected. Results As compared with those of the control group,Uterine wet weight,uterus organ coefficient,thickness of endometrium and smooth muscle,and the height of endometrial glands and glandular epithelium significantly increased in exposed group A and B (P

AIM: To study the effect of CPUE1 on the reversal of multidrug resistance (MDR) in K562/A02 cells and its mechanism. METHODS: MTT assay was used to determine the influence of CPUE1 on the cytotoxicity of vincrisine (VCR) in K562/A02 cells. The effect of CPUE1 on the apoptosis induced by VCR in K562/A02 cells was detected using DNA analysis and Annexin-Ⅴ/PI double stain assay. Flow cytometry was used to investigate the efflux of rhodamine123 (Rh123) by P-glycoprotein (P-gp) in K562/A02 cells. RESULTS: CPUE1 increased the cytotoxicity and apoptosis induced by VCR in K562/A02 cells. At the concentration of 10 ?mol?L -1 ,CPUE1 reduced the IC_ 50 value of VCR from 60.54 to 4.17 ?mol?L -1 . CPUE1 inhibited the efflux of Rh123 and resulted in the increase of intracellular accumulation of Rh123 in K562/A02 cells. CONCLUSION: CPUE1 has strong reversal effect on MDR in K562/A02 cells by inhibiting P-gp activity.