CD4 +T cells from patients with human immunodeficiency virus (HIV) infection undergo apoptosis at an enhanced rate, which leads to immune system damage and invalidation to withstand the virus intrusion. The current antiviral drugs aim at the virus can not clear the chronically infected cells which are the virus reservoirs. But treatment of HIV/AIDS patients with the HIV protease inhibitors (PIs) can reduce the cell apoptosis induced by HIV infection, which can improve immune function, and that is separate from the antiviral effects of PIs. Such suggests that immune reconstruction is a new strategy to cure AIDS. This text reviews HIV PIs research and development, their function characteristics and the effects on cell apoptosis. To define the relationship between HIV PIs and cell apoptosis may inspire new ways of curing the AIDS.-

Alzheimers disease (AD) is a progressively and retrogressively neurodegenerative disorder of central nervous system severely threatening the aged. The studies of animal models, which share the very similar neuropathological characteristics with human, provide tools for the understanding of the etiology, mechanisms and medicine screening of AD. The distribution and chemical composition of amyloid-beta (A- beta) peptide -positive deposits were investigated in various non-human primate models. Various proportions of A- beta deposits contained cholinesterase activities, apolipoprotein E(ApoE) and alpha1-antichymotrypsin immunoreactivity. Alterations in the basal forebrain cholinergic system have been widely studied, but the magnitude of decline and relationship to cognitive impairment are still a matter of debate. This article also includes the latest research of the changing on tau, subtle structural alterations in axons, and neurofibrillary tangles (NFT) in the brain tissues of the non-human primate models.

Objective To investigate the relationship between glucose variability and severity of coronary artery stenosis in patients with type 2 diabetes mellitus (T2DM).Methods One hundred hospitalized patients with T2DM(55 males and 45 females,39-81 years old) who had undergone angiography were included in the study from February 2012 to October 2012.They were divided into 2 groups:(1) 60 patients with coronary heart disease(CHD)as experimental group and (2) 40 patients without coronary heart disease as control group.All participants underwent selective coronary angiography to evaluate the severity of coronary artery stenosis (Gensini score) and continuous glucose monitoring(CGM) for 72 h.The correlation was analyzed between biochemical findings,and Gensini scores between the 2 groups.The comparison between 2 groups was performed with LSD test,correlation analysis with Pearson correlation and multiple stepwise regression analysis.Result Compared with the levels of largest amplitude of blood glucose excursion (LAGE),standard deviation of blood glucose (SDBG),mean amplitude of glycemic excursion (MAGE),postprandial glucose excursion (MPPGE),and absolute means of daily differences (MODD) in control group [(8.1 ± 2.1),(1.9 ± 0.5),(4.8 ± 1.3),(4.5 ± 1.4),and (2.4 ± 0.8) respectively],the levels in the experimental group[(11.3 ± 3.5),(3.2 ± 1.2),(7.0 ± 2.6),(6.4 ± 1.9),and (3.1 ± 1.3)] were higher respectively.MPPGE,MODD,LAGE,and C-reactive protein(CRP) were positively related to Gensini score(r were 0.498,0.246,and 0.227,all P＜0.05),while left ventricular ejection fraction (LVEF) was negatively related to Gensini score(r=-0.261,P＜0.05).Multiple stepwise regression analysis indicated that MPPGE and LVEF were independent risk factors of Gensini score (Y =59.727 + 11.779 × MPPGE-1.437 × LVEF).Conclusion Glucose variability in patients with T2DM and CHD was markedly enhanced.MPPGE was positively correlated with the severity of coronary artery stenosis in patients with T2DM and CHD.

Cancer stem cells are thought to be the seed of tumor formation, through complex signalings and cytokines in the surrounding microenvironment regulate the development and metastasis of tumor.Tumor stem cells have the characteristics of self-renewal and differentiation, the intracellular signaling pathways regulating self-renewal and differentiation of cancer stem cell include Wnt, Hedgehog signaling pathway.The tumor microenvironment is the dimensional environment surrounding the tumor,including the extracellular matrix, surrounding blood capillary,stromal cells ( fibroblasts, mesenchymal stem cells, tumor-associated endothelial cells, inflammatory cells) and cytokines secreted by stromal cells.Cancer stem cells maintain a close communication with the cells in the tumor microenvironment.In this paper, the cell surface maker of cancer stem cell,cancer stem cells and regulation of cytokine in the microenvironment,intracellular signaling pathways of cancer stem cells are reviewed to show complex regulatory networks in the tumor microenvironment.This review should help providing a new direction on specific cancer therapy for cancer stem cells in cacer treatment.

Objective To study the changes of intrapartum cesarean rate, cesarean indications and pregnancy outcomes after the new partogram applied. Methods Totally 3 290 pregnant women trying to vaginal delivery which were managed according to the new partogram in Beijing Obstetrics and Gynecology Hospital, Capital Medical University from August to October in 2014 (new partogram group) were involved;2 987 pregnancy women trying to vaginal delivery from May to July in 2014 which were managed according to the old partogram (old partogram group) were involved as control. The intrapartum cesarean rate, cesarean indications and the pregnancy outcomes between the two groups were analyzed. Results The rate of intrapartum cesarean delivery was 8.50%(254/2 987) in old partogram group, and was significantly higher than that in new partogram group (6.2%, 204/3 290;P<0.01). Cesarean indications in old partogram group included 18 labor protraction (7.1%, 18/254), 82 labor arrest (32.3%, 82/254), 44 relative cephalopelvic disproportion (17.3%, 44/254), 80 fetal distress (31.5%, 80/254), 23 intrauterine infection (9.1%, 23/254) and 7 cesarean delivery on maternal request (CDMR;2.8%, 7/254) . Cesarean indications in new partogram group included 33 labor arrest (16.2%, 33/204), 71 relative cephalopelvic disproportion (34.8%, 71/204), 73 fetal distress (35.8%, 73/204) and 22 intrauterine infection (10.8%, 22/204), 5 CDMR (2.5%, 5/204). There were no significant differences in incidence of asphyxia neonatorum and puerperal morbidity (P>0.05), but the incidence of postpartum hemorrhage in new partogram group was higher than the old partogram group [6.9% (14/204) versus 1.6% (4/254), P<0.05]. Conclusion After the new partogram applied, the rate of intrapartum cesarean delivery is significantly decreased, but the incidence of postpartum hemorrhage is increased.

Objective To investigate the present status of Chinese patent medicine with realgar (As2S2) and to research arsenic dissolution in different pH values. Methods Data on the amount of Chinese patent medicine with realgar, the content of realgar, the method of taking medicine and so on were collected from the Chinese traditional medicine protection and criterions issued by the Chinese Ministry of Health in 2005, and evaluated the using status of Chinese patent medicine with realgar. The realgar powder was dissolved in solutions at different pH values (1, 3, 5, 7, 9, 11) in 37℃ water for 4 h, respectively, then arsenic was determined with cold trap hydride generation atomic absorption spectrometry to calculate the amount of dissolved arsenic. Results One hundred and twenty-one (3.13%) realgar medicaments were recorded in a total of 3 860 various Chinese patent medicaments, including 74 medicaments with both realgar and cinnabar (HgS) . Of all medicaments with realgar, 97 medicaments (80.17%) were for oral application, 10 medicaments (8.26%) were for external application, and 14 medicaments (11.57%) were for both oral and external application. 45 medicaments with realgar (1.17%) could be used in children; 108 medicaments with realgar (89.26%) contained arsenic 15%; The relative amount of arsenic in medicaments with realgar was from 0.46% to 27.52%. Daily intake of arsenic was 500 mg for 11 medicaments (9.09%). Only inorganic arsenic (iAs) was detected when 1 mg realgar was dissolved in solution with pH values of 1, 3, 5, 7, 9, 11. The levels of dissolved iAs seemed increased with the pH values, which were 1.58 ?g, 1.24 ?g, 1.57 ?g, 1.62 ?g, 2.28 ?g and 4.76 ?g, respectively. Conclusion Considering the common use of realgar in Chinese patent medicine and the high level of arsenic in realgar, the potential danger can not be ignored. It is possible that arsenic in realgar may be much easier to be absorbed in intestine than in stomach.

Objective To investigate whether smoking affects metabolism and methylation of arsenic. Methods Six papers about the relation between smoking and arsenic metabolism, methylation were collected until December,2008, and the data were quantitatively analyzed with random and fixed effect models. Results In the group of smoking, the summary weighted mean difference of percent of inorganic in urinary was 0.59 (95% CI:-0.01-1.18). The summary weighted mean difference of percent of MMA in urinary was 2.44 (95%CI:1.95-2.94). The summary weighted mean difference of percent of DMA in urinary was -3.04 (95%CI:-4.01- -2.07). Current evidence suggested that percent of MMA was higher, percent of DMA was lower in smoking or ever smoking group compared with nonsmoking group. Conclusion Smoking may be considered a risk factor for metabolism and methylation of arsenic on human.

Objective To investigate the characteristics of electroencephalograms ( EEGs ) of patients with childhood absence epilepsy ( CAE ) .Methods EEG of 46 patients with CAE were analyzed .Results Of 46 patients,there were 44 cases (95.6%) with guided bilateral symmetry 3Hz spike-and-wave.16 cases were character-ized by multiple spike-and-wave on the 3Hz spike and slow wave;and the centrotemporal spikes was found in 1 case. Additionally,the 3Hzδactivity in occipital region has been traced in 34 cases of these 46 patients.Conclusion In general,the EEGs of CAE patients have typical characteristics ,and few of them showed atypical manifestation ,which must be verified by clinical attention .

This paper selects the bag-type infusion sets for single use as samples, which are produced by different manufacturers and based on the ethylene oxide sterilization. The ethylene oxide sterilization residues in different parts of samples are detected by colorimetric analysis. Combined the comparison of the ethylene oxide residues testing results in the different parts of the same sample with the actual situation in clinical use, more reasonable sampling positions are found to detect the ethylene oxide sterilization residues. The result of this experiment will play a guiding role in the detection of the actual samples.
Disposable Equipment ; Equipment Contamination ; prevention & control ; Ethylene Oxide ; analysis

Objective To explore the effects ofPolygonum multiflorum Thunb. by different processed temperature on acute liver injury induced by CCl4 in mice. MethodsA total of 140 KM mice were randomly divided into the control group, the positive drug group, the model group, the 100℃, 110℃, and 120℃ processed group and crude drug group, all the groups above were in sequence given the equal normal saline, silymarin (10 mg/kg), 100℃ extract (100 mg/kg), 110℃ extract (100 mg/kg), 120℃ extract (100 mg/kg) and crude drug extract (100 mg/kg) each day, respectively. After 7 days, apart of the control group, the other groups were established the acute liver injury model by the carbon tetrachloride. The blood and liver of mice were taken after 24 h post-model made. The levels of AST, ALT, Alb, TP, IL-6, TNF-a, IL-1, SOD, MDA and GSH-Px were detected, and liver pathological features of each group were observed.Results Compared with the model group, the levels of ALT (132.14 ± 19.66 U/L, 81.55 ± 12.67 U/L, 169.37 ± 24.18 U/Lvs. 261.84 ± 31.61 U/L), AST (231.57 ± 38.38 U/L, 181.73 ± 36.52 U/L, 318.36 ± 39.68 U/Lvs. 624.79 ± 49.98 U/L), IL-6 (10.35 ± 1.62 pg/ml, 6.26 ± 1.36 pg/ml, 12.57 ± 1.88 pg/mlvs. 18.73 ± 5.54 pg/ml), TNF-α (243.74 ± 13.02 pg/ml, 189.36 ± 9.85 pg/ml, 273.13 ± 14.64 pg/mlvs. 314.36 ± 29.67 pg/ml), IL-1 (235.36 ± 30.14 pg/ml, 208.07 ± 9.33 pg/ml, 48.21 ± 33.15 pg/mlvs.264.76 ± 32.55 pg/ml) in the 100℃, 110℃, 120℃ group significantly decreased (P<0.05). The levels of TP (69.16 ± 2.96 g/L, 74.14 ± 3.17 g/L, 65.73 ± 2.22 g/Lvs. 62.06 ± 2.65 g/L), Alb (35.86 ± 2.64 g/L, 36.67 ± 2.81 g/L, 34.06 ± 2.64 g/Lvs. 32.16 ± 2.05 g/L) in the 100℃, 110℃, and 120℃ group significantly increased (P<0.05). The levels of SOD (82.94 ± 6.21 U/mg, 101.33 ± 9.16 U/mg, 71.32 ± 5.15 U/mg vs. 66.22 ± 1.13 U/mg), GSH-Px (153.39 ± 15.23 U, 199.25 ± 12.04 U, 159.26 ± 17.18 Uvs. 64.79 ± 32.56 U) in the 100℃, 110℃, and 120℃ group significantly increased (P<0.05), while the level of MDA (0.96 ± 0.22 nmol/mg, 0.69 ± 0.13 nmol/mg, 1.29 ± 0.13 nmol/mgvs.1.71 ± 0.33 nmol/mg) in the 100℃, 110℃, and 120℃ group significantly decreased (P<0.05). Conclusions Different processed temperature ofPolygonum Multiflorum Thunb. water extract on liver injury in mice caused by carbon tetrachloride is not the same. The 110℃ processed temperature showd the best liver protection.