1.Literature Review of Pharmacoeconomics of Antitumor Drugs
China Pharmacy 2005;0(17):-
OBJECTIVE:To study the current situation on the research of pharmacoeconomics of antineoplastics.METHO-DS:The pharmacoeconomic research papers on antitumor drugs published in domestic academic magazines over the 10 years ever since 1995 were evaluated by tabulating of the items.RESULTS:Only a preliminary pharmacoeconomic study on antitumor drugs has been done,however,which are far from standardized and perfect.CONCLUSION:The pharmacoeconomic study on antitumor drugs in itself has its complexity,which should be standardized so as to make the study results applicable further.
2.Study on adrenomedullin synthesized in alveolar macrophages in patients with chronic obstructive pulmonary disease
Ping XU ; Aiguo DAI ; Yuanyuan LIU
Chinese Journal of Practical Internal Medicine 2001;0(04):-
Objective To investigate the effects and mechanisms of adrenomedullin(ADM)synthesized in alveolar macrophages in patients with chronic obstructive pulmonary disease.Methods The bronchoalveolar lavagae fluids(BALF)were collected by bronchoscopy in 15 cases of COPD and 14 healthy cases.The expression of ADM in plasma,BALF and the culture supernatants of alveolar macrophages(AMs)were analysed by radioimmunoassay.Results (1)The cells,PMNs and AMs in BALF of COPD group were significantly higher than those of healthy group(P
3.Progress on necrotizing pneumonia in children
Lingman DAI ; Yuanyuan ZHANG ; Zhimin CHEN
International Journal of Pediatrics 2021;48(3):163-167
Necrotizing pneumonia(NP)is a serious pulmonary complication of community acquired pneumonia(CAP), and can occur in adults and children.NP was first reported in adults, and in recent years the incidence of NP in children is growing.Until now, there is little description compare the etiology, clinical manifestation, auxiliary examination, risk factors, treatment and prognosis in children and adults with necrotizing pneumonia.This article systematically reviews several aspects of NP between children and adults, which would be helpful for diagnosing and treating NP in different age groups.
4.Exploration of the regulation site of HIF-1α in Notch signaling pathway in the hippocampus of rats with status ;epilepticus
Yafei ZHUANG ; Lin ZHANG ; Baoqiang YUAN ; Yuanyuan DAI ; Rui LI
Journal of Clinical Pediatrics 2016;34(7):544-548
Objective To study the expression of hypoxia inducible factor-1 (HIF-1 ) and Notch signaling pathway downstream gene HES 1 in the hippocampus of pubertal rats with status epilepsy (SE), and to explore the regulation site of HIF-1αin Notch signaling pathway. Methods One hundred and seventy-six 21-day-old SD rats were randomly divided into control group (NS group), pentetrazole (PTZ)-induced SE group (PTZ group), and Notch signaling pathway speciifc inhibitor (DAPT) intervention group (DAPT group). In PTZ group PTZ was intraperitoneally injected to build SE model and in NS group normal saline was injected as control. The intraperitoneal injection of diazepam was used to terminate SE seizures. After successful modeling, the bilateral hippocampuses were isolated after the rats were sacriifced at 0.5, 1, 2, 4 and 8 h, respectively, and RT-PCR was performed to detect the mRNA expression of HES 1 and HIF-1α. The Western Blot was performed to detect protein expression in hippocampuses which were collected at 2 , 4 , 8 , 12 , and 24 h after successful modeling. DAPT group received intraperitoneal injection of DAPT 30 min before the start of molding, then the hippocampuses were isolated at 2 and 8 h after successful modeling. RT-PCR was performed to detect the mRNA expression of HES 1 and HIF-1αat 2 h, and Western blot was performed to detect protein expression at 8 h. Results At each time point after SE, the expression of mRNA of HES 1 and HIF-1αand the expression of protein were higher than the same time point of NS group (P0 . 05 ). Compared with the same time point of PTZ group, the mRNA expression of HES 1 and HIF-1αand the expression of protein of DAPT group were obviously reduced (P0 . 05 ). Conclusion HES 1 gene may be the regulatory site of HIF-1 expression in Notch signaling pathway in the hippocampus of puberty rats with SE.
5.Significance of cerebrospinal fluid matrix metalloproteinase-9, interleukin-6, and tumor necrosis factor-alpha protein in children with viral encephalitis
Donglin SHEN ; Yuanyuan DAI ; Jiao CHEN ; Ming LU ; Mingxia SUN
Chinese Journal of Applied Clinical Pediatrics 2014;29(22):1721-1723
Objective To explore the significance of changes of matrix metalloproteinase-9 (MMP-9),interleukin-6 (IL-6),and tumor necrosis factor-alpha (TNF-oα) protein level in the cerebrospinal fluid (CSF) of children with viral encephalitis (VE).Methods The concentration of neuron-specific enolase (NSE),structural proteins 100B (S100B),and MMP-9,IL-6,TNF-α in the CSF of VE children were detected by an enzyme linked immunosorbent assay,and the correlations of them were analyzed.Results NSE,S100B,MMP-9,IL-6,TNF-α protein expression could be found significantly higher than those in the control group,and there were significant differences according to statistics expression trends(all P <0.05).The NSE protein expression was significantly positive related with S100B in the VE group (r =0.467,P =0.009),and the concentration was markedly negative related with the duration of viral encephalitis (r =-0.472,P =0.008).MMP-9,IL-6 protein expression were significantly positive related with NSE,S100B respectively (r =0.698,P =0.00 ; r =0.559,P =0.00 ; r =0.812,P =0.00 ; r =0.664,P =0.00).TNF-α protein expression was positive related with CSF S100B(r =0.363,P =0.049),but there was no correlation between TNF-α and NSE (r =0.245,P =0.193).Conclusions The neurons and the neuroglial cells are damaged in the viral encephalitis children.MMP-9,IL-6,TNF-α protein may participate in the pathological damage process of nerve cells in VE children in different degrees.
6.Study on Pharmacokinetics of Levofloxacin Polymorphs in Rats
Yuanyuan WU ; Guimin JIN ; Guanhua DU ; Yang LYU ; Guidong DAI
Herald of Medicine 2014;(11):1407-1411
Objective To study the pharmacokinetics difference of levofloxacin polymorphs in rats, evaluate the advantageous medical polymorph,and explore the effects of different polymorphs on clinical medicine. Methods Four crystal forms of levofloxacin were administered intragastrically to rats,and high performance liquid chromatography( HPLC)was used to measure the contents of levofloxacin in rat plasma. The pharmacokinetic parameters were calculated and compared Results After a single oral dose,the peak plasma concentration(Cmax)of crystal forms ofⅠ,Ⅱ,ⅢandⅣof levofloxacin was 6. 984,9. 692,9. 405,6.424 mg·L-1;the time to peak(tmax)was 0.6,0.9,1.0,1.0 h;the half-life(t1/2)was 4.207,2.97,4.857,1.695 h;theareaunderthecurve(AUC0→12h)was31.478,42.385,32.406,31.636mg·h·L-1. Conclusion Thereisnostatistically significant difference in pharmacokinetic parameters. However,compared with other crystal forms,plasma concentration of crystal form II is higher and maintained longer. Therefore,crystal form II of levofloxacin is an advantageous polymorph for medicine.
7.Effects of ketamine on the expression of adhension molecular CR3 and intracellular cAMP, CGMP of neutrophils in patients assoeiated with CPB
Anlu DAI ; Xiaowen GUO ; Fengjiang ZHANG ; Yuanyuan YAO ; Min YAV
Chinese Journal of Emergency Medicine 2008;17(7):738-741
Objective To evaluation the efficacy of ketamine on the expression of adhension molecular CR3and intracellular cAMP, cGMP in neutrephils in patients associated with cardiopulmonary bypass (CPB), as well as the cardiovascular function of the CPB patients. Method Sixty patients operated on with prosthetic valve replace-rnent under CPB were randomly divided into 4 groups: placebo, ketamine 0.1 mg/kg ( ketamine Ⅰ) ,ketamine 0.5 mg/kg ( ketamine Ⅱ) ,ketamine 1 mg/kg( ketamine Ⅲ). Each group included 15 eases. Venous blood sam-pies were obtained during anesthesia induction (T1), 10 min before CPB (T2), end of CPB (T3) and 24 hoursafter operation (T4). The expression of CR3 was measured by Flow cytometry and the concentration of cAMP/cGMP by HPLC. Results Ketamine with various dosages decreased the expression of CR3 at the T3 and T4 inpatients of ketamine groups compared with patients of placebo group (P<0.05). The dosages of ketamine Ⅱgroup and ketamine Ⅲ group had more significant effect than that of ketamine Ⅰ group. The dosages of ketamineⅡ and ketamine Ⅲ group increased the intracellular cAMP at the T3 and T4 compared with ketamine Ⅰ groupand placebo (p<0.05), respectively. However,cGMP was lower in ketamine Ⅱ and ketamine Ⅲ group thanthat in ketamine Ⅰ group and placebo (P<0.05) at the T3.Morever,the mean arterial blood pressure was higherin ketamine Ⅱ and ketamine Ⅲ group at T4. Only the patients of ketamine Ⅲ group required less inotropic drugsafter operation. Conclusions Ketamine can reduce the expression of adhhension molecular CB3 and intracellularcAMP, cGMP in neutrophils from patients associated with CPB.
8.Adrenomedullin relieves the pulmonary hypertension in hypoxic rats
Ping XU ; Aiguo DAI ; Yuanyuan LIU ; Jiali PI ; Weidong SONG
Basic & Clinical Medicine 2006;0(06):-
Objective To investigate the effects and mechanisms of adrenomedullin (ADM) and adrenomedullin receptor (ADMR) on hypoxia pulmonary hypertension (HPH) in rats.Methods Eighteen male Wistar rats were randomly divided into three groups:control group, hypoxic group and hypoxic plus ADM group.ADM was subcutaneously administered into rats with ADM. After 21 days, The mean pulmonary arterial pressure (mPAP) and right ventricular hypertrophy index[RV/(LV+S)] were measured. Hypoxic pulmonary vascular remodeling(HPSR) was observed with morphometric analysis. The level of pulmonary arterial plasma ADM was measured by radioimmunoassay. The semiquantitate in situ hybridization was used to measure the expression of ADMR mRNA in pulmonary artery walls. Results (1) The level of mPAP, RV/(LV+S),the ratio of vascular wall thick ness to external diameter(MT%) and ratio of vascular wall area to total area (MA%) were significantly higher in hypoxia group than those in control group(2) The pulmonary artery plasma ADM level was significantly higher in hypoxic group and hypoxic plus ADM group than that in control group(P
9.Evaluation of methods for detecting susceptibility of Staphylococcus aureus to Vancomycin
Xiaoling MA ; Tao ZHANG ; Yuanyuan DAI ; Jjianzhong CHEN
Chinese Journal of Clinical Laboratory Science 2006;0(03):-
Objective To investigate the effective methods for detecting the susceptibility of Staphylococcus aureus to Vancomycin. Methods An isolate of h-VRSA (heterogeneous Vancomycin-resistant Staphylococcus aureus) isolated from clinical specimen was inoculated on the increasing concentration of vancomycin agars. Varied degree of vancomycin of resistant S. aureus were obtained. The susceptibilities of these S. aureus to vancomycin were tested by agar screening test, broth microdilution method, E-test, disk diffusion method and automated methods. Results Agar screening test, broth microdilution method and E-test are effective for detection of vancomycin-intermediate S. aureus VISA, but disk diffusion and automated methods could not detect VISA. Conclusion Broth microdilution method and E-test are acceptable methods for susceptibility testing of S. aureus to vancomycin. The laboratories using automated methods and disk diffusion method as routine susceptibility test should consider adding a vancomycin agar screen plate to enhance detection of VRSA and VISA.
10.Talking on Human Parasitology's Scientific Orientation in Preclinical Medicine Education
Jinchuan LI ; Kang CAO ; Fuying DAI ; Yuanyuan WANG ; Yue QIU
Chinese Journal of Medical Education Research 2006;0(08):-
Now human parasites are still important pathogens to harm human health.Researches on parasites have ranged from the simple aetiology to the field of studying vital phenomena by modern science.However some domestic medical colleges cut out the course of human parasitology without enough consideration.Aiming directly at this phenomenon the author has discussed the scientific orientation of human parasitology in preclinical medicine education and expounded the point of view.