The peripheral venous plasma levels of TXB_2 and 6-Keto-PGF_(1?) were determined by radioimmunoassay in 31 control subjectsandand 51 patients with acute cerebral infarction(ACI).In ACI patients, the plasma TXB_2 levels were significantly higher than those in control subjects (P

Acute ligation of the bilateral common carotid artery and left vetebro-ar-tery resulted in an experimental incomplete cerebral ischemia in rabbits. It was consideredthat such experimental model could be used for studying the pathogenesis of brainischemia and the effect of experimtal therapy. 654-2 (anisodamine) was given at 30 and 60 min. after ischemia. The results indi-cated that: (1) The reducing rate of the cerebral blood flow in 654--2 group after ischemiawas much lower than that in control group (P

Acute ligation of the common carotid artery on one side internal carotid artery on opposite side resulted in experimental cerebral ischemia in rabbit. Measurement of thromboxane B_2 (TXB_2) and 6-Keto-prostaglandin F_(1?)(6-Keto-PGF_(1?))in rabbit plasma were done by radioimmunoassay. Blood samples were collected from the peripheral vein and internal carotid vein. The results were as follows:1. The concentration of TXB_2 in rabbit cerebral ischemia (ligation after 30 min.) was significantly (P

As the investments from the state and society in universities increased rapidly, the scientific and technological resources gained huge promotion. However, the efficiency of some instruments remained low, and the repeated purchases of some instruments occurred frequently. Here the authors discussed about building a practical, reasonable and efficient system for lab management.

BACKGROUND: Research has proved that nifedipine can improve the functions of vascular endothelial cells. However, it still needs further study on its mechanism on dilating vessels.OBJECTIVE: To observe the effects of controlled-release nifedipine to nitrogen monoxide(NO) and induced nitric oxide synthase(iNOS) in spontaneously hypertensive rats(SHR).DESIGN: Comparative observational study based on experimental animals.SETTING: Experimental animal centre in a medical college.MATERIALS: This study was completed in Experimental Animal Centre of Shandong University during April to May 2002. Twenty-one recombinant inbred rats of clean class with body mass of(300 ± 2) grams were provided by Experimental Animal Centre of Shandong University. They were randomly divided into three groups: control group, normal group and low dose group,each with 7 rats.METHODS: Saline(10 mL/kg), 10 mL/kg and 3 mL/kg controlled-release nifedipine solution(0. 3 g/L) were poured into stomach to rats in control group, normal dose group and low dose group once everyday for 15 days respectively. Eyeball was removed to collect blood and heart and lung were removed at the same time to test the content of NO and iNOS in serum after last administration.MAIN OUTCOME MEASURES: Content of NO, comparison of iNOS activity between groups.RESULTS: There was significant difference on NO content between normal dose group and control group, low dose group after 15 days' administration ( P < 0. 01 ) . Compared with control group and low dose group, there was significant difference on the iNOS activity in heart and lung of normal dose group(P <0.01, P <0.05).CONCLUSION: While nifedipine reduces the blood pressure, it can also improve the content of NO and resist the increasing activity of iNOS casued by increased blood pressure.

This study examined the cytotoxicity of a new implant material modified by microarc oxidation technique. Cells on different surfaces of the implant were evaluated 2, 4 and 6 days after treatment. The results showed that cell attachment, cell morphology, and cell proliferation were influenced by the different surface treatments, and a significant increase in the osteoblast cell activity was observed on the porous MAO-Ti coating. Our results suggest that the porous MAO-Ti surface has a better biocompatibility and electrochemical performance than pure titanium surface.

Objective To evaluate the effect of AIDS health education in university students to provide the theoretical basis for AIDS prevention in the young college students .Methods In order to know about the situation of awareness HIV/AIDS ,a general survey of HIV/AIDS related knowledge was conducted in freshmen of Dali college by self‐made questionnaire In December 2010 , then various forms of health education was took for college students ,such as theoretical study ,lecture and so on .Investigation a‐mong students once again ,and compared with the baseline survey results in December 2012 .Results The accuracy of HIV/AIDS health knowledge in the young college students after had health education was higher significantly than that before(P<0 .05);The score of AIDS elated problem have been weighted ,and awareness was defined as≥60 ;Before health education ,2 619 students knew about AIDS related knowledge ,872 students did not ,the average score was (64 .50 ± 10 .22) ,the total awareness rate was 75 .67% ;after the health education ,3 323 students know about AIDS related knowledge ,138 students did not ,the average score was (84 .94 ± 12 .68) ,the total awareness rate was 96 .01% .After continuing health education ,the total score was significantly increased (P<0 .01) .Conclusion The awareness rate of AIDS could be improved by health education in various forms and that was impor‐tant for the prevention of AIDS among college students .

To investigate the distribution of hepatitis B virus (HBV) genotypes and subgenotypes among the Bai nationality in Dali, a total of 100 serum samples from patients with chronic HBV-infection were collected for the detection of HBV genotypes and subgenotypes by genotype-specific primers and restriction fragment length polymorphism (RLFP), respectively. Among the 100 samples, the proportions of genotype B, C and mixed genotype (B+C) were 41%, 25% and 34%, respectively. All the genotype B strains belonged to subgenotype Ba. In genotype C, 84% were Subgenotype Cs and 12% were subgenotype Ce. The distribution of genotypes B, C and B+C showed no significant difference between male and female patients (P=0.182) and among the age groups of patients (P=0.812). The rates of HBeAg/HBeAg positivity were no significantly different among genotypes B, genotype C and mixed genotype (B+C) (P=0.077/P=0.663). In Dali, genotypes B, B+C and C existed among Bai nationality with chronic HBV-infection, and genotype B was the major genotype. Subgenotypes Ba and Cs were the predominant strains in patients with HBV genotype B/C infection. The most prominent characteristic was the higher prevalent rate of mixed genotype (B+C) in patients.

Objective: To investigate the antiamnesic effect of pregnenolone sialic glycoside on memory impairment in mice. Methods: Pharmacological model of amnesia in mice was produced by scopolamine. Learning and memory was evaluated using water maze performance of mice. Results: Significant memory deficit was induced by scopolamine (4 mg/kg, ip). Pregnenolone sulfate (0.1 5 mg/kg, sc ) failed to affect the scopolamine induced memory deficit. Pregnenolone sialic glycoside (0.1 5 mg/kg, sc) attenuated the deficit, decreased the time of finding platform, the errors of entering non exit and swimming distance at a dose of 1 mg/kg ( P

Background and Objective
  The prevalence of non-communicable diseases (NCD) is increasing in low- and middle-income countries, imposing major public health and development threats. However, there is difference among countries with regard to the patterns of NCD metabolic risk factors. This study aims to categorize the pattern of metabolic risk factors in East Asia, Southeast Asia and Oceania.
Methods
  Age-standardized prevalence of obesity, raised blood pressure, raised blood glucose, and raised blood cholesterol for 2008 were obtained from the World Health Organization (WHO) Global Health Observatory Data Repository. We used hierarchical cluster analysis to categorize countries in East Asia, Southeast Asia and Oceania based on the prevalence of NCD metabolic risk factors of each country.
Results
  Three patterns of NCD metabolic risk factors were identified. The first pattern showed relatively high prevalence of raised blood cholesterol, while prevalence of obesity, raised blood pressure and raised blood glucose remain relatively low. Most high- and upper-middle-income Asian countries were included in this pattern. The second pattern presented relatively high prevalence of raised blood pressure, although prevalence of obesity, raised blood glucose, and raised blood cholesterol stay relatively low. Most low- and lower-middle-income Asian countries were categorized in this pattern. The third pattern presented high prevalence of obesity and relatively high prevalence of raised blood pressure and raised blood glucose. This pattern included most Pacific island countries.
Conclusions
  Policy makers in countries in East Asia, Southeast Asia, and Oceania should take into account for the features of the pattern they are in, when they set priorities for developing effective NCD control measures.