The stress fracture is often resulted from forced march, heel-and-toe walking race, trudge over a longdistance etc. The stress fracture in metatarsal bone is very common. After analysis of bearing-force onmetatarsal bone, the feet are simplified as weight-bearing-cantilever beam structure, while the metatarsalbone as lever units which support the bending cantilever. 16 metatarsal bones were taken from freshcadavers of normal Chinese adults. TYPE 2H cantilever stress teeting machine was used for the experiment.This study analyzes the injury mechanism of stress fracture theoretically, the defines limit of stress fractureof the metatarsal bone, and makes biomechanical analysis for the fracture types. This experiment providesthe data and materials for prevention and diagnosis of stress fracture and the possibility of methods andpaths for stress fracture experiment of the biomaterials.

Objective To observe the effects of lower limb motion control training using virtual games combined with core stability training (CST) on the lower limb motor function of hemiplegic patients after stroke.Methods Sixty hemiplegic stroke patients were divided into an observation group and a control group (n =30 in each) using a random number table.The control group was given conventional treatment and CST,while the observation group received lower limb motion control training using virtual games in addition to conventional treatment and CST.All of the patients were assessed using the Fugl-Meyer lower limb assessment (FMA),the Berg balance scale (BBS),the modified Barthel index (MBI),10 metre maximum walking speed (MWS) and functional ambulation categorization (FAC)before and after 8 weeks of treatment.Results Before the intervention there was no significant difference between the two groups in any of the measurements.After the treatment,however,the average FMA,BBS,MBI,MWS and FAC in the observation group were significantly better than those before treatment and those in the control group.Conclusions Lower limb motion control training using virtual games combined with core stability training can improve effectively the lower limb motor function of hemiplegic patients after stroke.Such a combination is worth applying in clinical practice.

Objective:To observe the clinical effect and safety of lauryl alcohol injection under the guidance of ultrasound in the treatment of thyroid cyst.Methods:From February 2017 to February 2019, 148 patients with thyroid cyst who had been treated in the First People's Hospital of Huzhou were divided into study group and control group according to the random number table method, with 74 patients in each group.The patients in the study group were treated with ultrasound-guided percutaneous injection of lauryl alcohol, while the patients in the control group were treated with ultrasound-guided percutaneous injection of anhydrous ethanol.The changes of serum free triiodothyronine(FT 3), free tetraiodothyronine(FT 4) and thyroid-stimulating hormone(TSH) were compared before treatment and after treatment for 6 months.The curative effect was evaluated and the adverse reactions were observed after treatment. Results:There were no statistically significant differences in the serum TSH, FT 3 and FT 4 between the two groups before and after treatment( t=-0.514~0.624, P=0.513~0.852). The overall effective rate in the study group(94.59%) was significantly higher than that in the control group(82.43%)(χ 2=5.382, P=0.020). The overall incidence of adverse reactions in the study group(12.16%) was significantly lower than that in the control group(31.08%)(χ 2=7.815, P=0.005). Conclusion:Ultrasound-guided injection of lauryl alcohol and anhydrous ethanol did not affect the thyroid function of patients with thyroid cysts, while ultrasound-guided injection of lauryl alcohol is conducive to improve the efficacy of thyroid cysts, and the safety is higher, which is worthy of clinical application.

OBJECTIVE: To identify the pathogenesis in two patients of restrictive cardiomyopathy (RCM) using high-throughput sequencing. METHODS: Peripheral blood samples from the two patients and their parents were collected and genomic DNAs were extracted to conduct targeted next generation sequencing or whole exome sequencing. Bioinformation analysis was performed to identify the pathogenic variants in genes associated with cardiomyopathy, which were further validated by Sanger sequencing. RESULTS: By high throughput sequencing, we detected a de novo heterozygous variant c.549+1G>T in TNNI3 gene in patient 1. The variant has not been reported previously and was predicted to be pathogenic in line with American College of Medical Genetics and Genomics (ACMG) guidelines (PVS1+PS2+PM2). Another heterozygous variant c.433C>T (p.Arg145Trp) in TNNI3 gene was identified in patient 2 and his father. The variant had been reported as pathogenic variant in Clinvar and HGMD databases; based on ACMG guidelines, the variant was predicted to be likely pathogenic (PS3+PM1+PP3). CONCLUSION TNNI3 variants may be the causative gene responsible for restrictive cardiomyopathy in the two patients. High throughput sequencing results provide bases for the diagnosis of restrictive cardiomyopathy.
Cardiomyopathy, Restrictive/genetics* ; Child ; Genomics ; Heterozygote ; Humans ; Mutation ; Whole Exome Sequencing