1.Study Progress on Biosensing Core/shell Nanoparticles
Jie LUO ; Guangming ZENG ; Lin TANG ; Juan YIN ; Yuanping LI
Chinese Journal of Analytical Chemistry 2009;37(12):1847-1852
Recently, more and more public attention has been paid to nanomaterials in various fields. Especially, the preparation methods of core/shell nanoparticles have been drastically updated and developed. There exists great application prospect for the development of biosensing core/shell nanoparticles. This paper emphatically introduced the operation principle, preparation methods of biosensing core/shell nanopaticles and the latest application progress in electrochemical biosensor, optical biosensor and piezoelectric crystal biosensor.
2.Vagus nerve preserving pericardial devascularization with subtotal splenectomy for the treatment of portal hypertension
Jiancheng LIU ; Yuanping ZENG ; Jian LAI ; Jianhua QIU ; Haifeng HUA ; Min XIAO ; Xiaoping LIU
Chinese Journal of General Surgery 2015;30(10):770-773
Objective To investigate the clinical effect of vagus nerve preserving pericardial devascularization plus subtotal splenectomy in treating portal hypertension with a history of variceal bleeding.Methods The clinical data of 33 cases of portal hypertension with variceal hemorrhage treated with vagus nerve preserving selective pericardial devascularization plus subtotal splenectomy from April 2004 to December 2013 (study group) were compared with that of 34 cases treated with pericardial devascularization plus splenectomy (control group).Results There was no mortality in two groups.The postoperative gastric drainage during the first 72 h were(1 525 ±30) ml in the study group and (2 130 ±40) ml in control group(P <0.05).Portal vein thrombosis developed in one case in the study group and 15 cases in the control group(P < 0.05).Postoperative 3-year recurrent gastroesophageal varices hemorrhage was 15% in the study group and 25% in the control group (P < 0.05).Postoperative 5-year variceal hemorrhage recurrence rate were 28% in the study group and 30% in the control group (P > 0.05).Conclusions Maitaining vagus nerve selective pericardial devascularization plus subtotal splenectomy is of less postoperative complication and lower portal vein thrombosis rate and better patients' survival compared with pericardial devascularizatim plus total splenectomy.
3.Association between viral load and gestational diabetes mellitus in women with chronic hepatitis B
Feifeng LI ; Qitao HUANG ; Chen HUANG ; Yuanping ZHOU ; Zhihua LIU ; Weijuan ZENG ; Haizhen WANG ; Guosheng YUAN ; Mei ZHONG ; Yunfei GAO
The Journal of Practical Medicine 2016;32(19):3117-3119
Objective To analyze the GDM of 336 cases with chronic HBV in pregnancy. Methods According to HBV DNA≥1.0 × 103 IU/mL, participants were divided into HBV DNA (+) or (-) group. 409 cases without HBV were selected as control group. Differences on GMD incidence between groups and virus load and OGTT blood sugar correlation were compared. Results The incidence of GDM of HBV DNA (+) or (-) group was 16.77% and 17.71%, which is higher than that in HBV group (10.27%). The difference is significant (P < 0.05). The correlation index between HBV DNA and fasting blood-glucose is r = 0.005, P = 0.610, the result of which is not statistically significant. But correlation index between HBV DNA and blood sugar at 1 h , 2 h are r = 0.082, 0.086; P = 0.000, 0.000, the result of which is statistically significant. Conclusion The oc-currence of GDM were higher in HBV DNA (+) or (-) group. The viral load is positively related with blood sugar of glucose tolerance at 1 h or 2 h.
4.Construction and sequence analysis of recombinant HCV-1b replicon by replacing NS5A region.
Jingtao LI ; Bin ZHOU ; Yuanping ZHOU ; Yanli ZENG ; Wei LI ; Junjie WANG ; Jian ZHANG ; Hao ZHANG ; Shuwen LIU
Journal of Southern Medical University 2012;32(1):46-49
OBJECTIVETo construct the recombinant HCV-1b replicon by replacing NS5A region using serum samples from patients with chronic hepatitis C (CHC) in South China and explore the biological characteristics of NS5A protein in response to antiviral therapy.
METHODSThe on-off plasmid containing the cutting sites of the restriction endonucleases MIu I and Bcl I was designed based on the backbone of robust HCV 1b replicon. The full-length fragments of HCV NS5A were amplified from different CHC patients by RT-PCR and cloned into pMD-18 vector, followed by sequence analysis of amino acid mutation of ISDR, PKRBD, V3 and IRRDR within the NS5A region. If the amplicon obtained contained no MIu I or Bcl I cutting sites, the NS5A fragment was re-amplified using primers containing the cutting sites and inserted into the replicon for replacement.
RESULTSThe full-length fragments of NS5A were obtained successfully from CHC patients. The core region of ISDR-V3 of NS5A was replaced in the HCV replicon plasmid and showed correct sequences. The amino acid mutations of ISDR and PKRBD within NS5A were more frequent in patients with sustained viral response (SVR) than those without SVR. A high variability in the amino acid sequence was observed in both IRRDR and V3 regions.
CONCLUSIONThe plug-in type recombinant HCV replicon for replacement of NS5A region in the virus from CHC patients has been successfully constructed, which provides a basis for further investigation of the biological characteristics of NS5A protein, the mechanisms of interferon-resistance, and antiviral therapy of difficult-to-treat CHC.
Amino Acid Sequence ; Antiviral Agents ; administration & dosage ; pharmacology ; Drug Resistance ; genetics ; Genetic Variation ; Genotype ; Hepacivirus ; genetics ; Hepatitis C, Chronic ; drug therapy ; virology ; Humans ; Interferons ; administration & dosage ; pharmacology ; Molecular Sequence Data ; Recombination, Genetic ; Replicon ; genetics ; Sequence Analysis ; Viral Nonstructural Proteins ; genetics
5.Efficacy evaluation of specific immunotherapy with standardized house dust mite allergen preparation for allergic rhinitis.
Jie ZHOU ; Yuanping HE ; Xudong ZENG ; Zewen LI ; Fangming PAN ; Yingchao CHEN ; Zhaolong LI
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2012;26(11):487-489
OBJECTIVE:
To evaluate the efficacy of specific immunotherapy (SIT) with standardized house dust mite allergen preparation for allergic rhinitis (AR).
METHOD:
Fifty-five patients with allergic rhinitis caused by house dust mites were selected in this self-control study. Clinical efficacy was evaluated by symptom and sign score after two years of specific immunotherapy and compared with pre-treatment scores.
RESULT:
After completing the study, a clinically significant reduction in symptom and sign score in these patients was noted, compared with that of pretreatment. And the difference was statistically significant (P < 0.01).
CONCLUSION
Standardized house dust mite allergen preparation is an effective treatment in patients suffering from allergic rhinitis due to house dust mites.
Allergens
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therapeutic use
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Animals
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Antigens, Dermatophagoides
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therapeutic use
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Female
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Humans
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Immunotherapy
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methods
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Male
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Pyroglyphidae
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immunology
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Rhinitis, Allergic
;
therapy
;
Treatment Outcome
6.Value of nasal endoscopy in the surgery of the nasal inverted papillomas.
Fubo YAN ; Xudong ZENG ; Yuanping HE ; Qingyuan ZHANG ; Wu WANG ; Wei WANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2009;23(11):494-496
OBJECTIVE:
To evaluate the curative effect of endoscopic surgery (ESS) in the treatment of inverted papillomas in the nasal cavity and paranasal sinus.
METHOD:
We report on 16 clinicopathologic cases of inverted papilloma. After preoperative endoscopic examination and CT screening, endoscopic sinus surgery was performed under general anesthesia.
RESULT:
The follow-up period ranged between one year to two years. Recurrence was observed in 2 cases.
CONCLUSION
The nasal endoscopy is a better method for treating Krouse I-II stage nasal inverted papilloma. The advantages of endoscopic surgery include clear visual field, less bleeding, limited reoccurrence, and avoidance of facial incision.
Adult
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Aged
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Endoscopy
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Female
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Humans
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Male
;
Middle Aged
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Neoplasm Staging
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Nose Neoplasms
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surgery
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Papilloma, Inverted
;
surgery
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Treatment Outcome
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Young Adult