1.Application of real-time fluorescence isothermal RNA amplification assay in the determination of Mycobacterium tuberculosis in pleural effusion
Song CHENG ; Chengyong LIU ; Dongqing ZHOU ; Yuanpei HOU
International Journal of Laboratory Medicine 2015;(18):2697-2698,2701
Objective To evaluate clinical value of utilization of real‐time fluorescence isothermal RNA amplification assay (SAT)for detecting Mycobacterium tuberculosis(MTB) in pleural effusion samples of patients with tuberculous pleurisy .Methods 90 hydrothorax specimens of patients with tuberculous pleurisy and 52 hydrothorax specimens of patients with non‐tuberculous pleurisy were selected ,and MTB was detected by using Roche culturing method ,SAT and TB DNA fluorescence quantitative expan‐sion method respectively .The sensitivity ,specificity and clinical value of SAT for detecting MTB were analysed .Results Taking Roche culturing method as the standard ,the sensitivity ,specificity and Youden index of SAT for detecting MTB were 90 .9% , 72 .1% and 0 .630 respectively .In patients clinically diagnosed with tuberculous pleurisy ,the positive detection rate of hydrothorax specimens detected by using Roche culturing method and SAT were 24 .4% and 43 .3% respectively ,and had statistically significant differences(χ2 =7 .166 3 ,P=0 .001 4) .Conclusion SAT is a rapid ,sensitive and specific method for the detection of MTB in pleu‐ral effusion samples ,which could improve the detection rate of MTB and decrease incidence of missed diagnosis and misdiagnosis .
2.Protective effect of supplementation with Lycium ruthenicum Murray extract from exhaustive exercise-induced cardiac injury in rats.
Chien-Wei HOU ; I-Chen CHEN ; Fang-Rui SHU ; Chin-Hsing FENG ; Chang-Tsen HUNG
Chinese Medical Journal 2019;132(8):1005-1006
Animals
;
Cardiovascular Diseases
;
drug therapy
;
etiology
;
Creatine Kinase, MB Form
;
blood
;
Heart
;
drug effects
;
Interleukin-1
;
blood
;
Interleukin-6
;
blood
;
Lycium
;
chemistry
;
Male
;
Nitrates
;
blood
;
Oxidative Stress
;
drug effects
;
Physical Conditioning, Animal
;
adverse effects
;
Plant Extracts
;
therapeutic use
;
Rats
;
Rats, Sprague-Dawley
;
Reactive Oxygen Species
;
blood