BACKGROUND:Periodontal tissue engineering technology provides new ideas and new ways for periodontitis-induced bone defect repair. OBJECTIVE:To develop a culture model for the periodontal ligament cells of miniature swine, which was constructed with hydroxyapatite, to investigate the biocompatibility with hydroxyapatite. METHODS:Periodontal ligament cells from miniature swine were harvested by using tissue explant method. Immunofluorescence was used to detect the expression of stromal cel antigen 1 in the periodontal ligament cells of miniature swine. The third passage cells were co-cultured with a three-dimension hydroxyapatite scaffold, and the biological characteristics of the cells were observed under a scanning electron microscope at days 1, 3, 7 of co-culture. RESULTS AND CONCLUSION:The pirmary miniature swine periodontal ligament cells grew wel , and they were positive for stromal cel antigen 1. Under the scanning electron microscope, the periodontal ligament cells of miniature swine grew wel on the hydroxyapatite scaffold at days 1, 3, 7 of co-culture. These prove that the miniature swine periodontal ligament cells, which can be separated using tissue explant method and cultured successful y in vitro, can grow wel on the hydroxyapatite scaffold.

Objective To identify the potential correlation of serum 25-hydroxyvitamin D3 [25 (OH)D3]and C-reactive protein (CRP) with coronary artery disease.Methods We measured the serum 25 (OH)D3 and CRP levels in 416 suspected coronary heart disease patients who underwent coronary angiography.Gensini scores were used to assess the severity of coronary stenosis.Based on the results of coronary angiography,the 416 patients were divided into normal group (n =246),stable angina group (n =110),and acute coronary syndrome (ACS) group (n =60).Results The serum levels of 25 (OH) D3 in the normal group and stable angina group were significantly higher than that in the ACS gropu [(33.15 ± 20.95) nmol/L vs.(15.55 ±5.7) nmol/L,(28.93 ± 16.45) nmol/Lvs.(15.55 ± 5.7) nmol/L,bothP＜0.001].TheCRPlevelin the normal group was significantly lower than those in the stable angina group and the ACS group [(4.33 ± 0.12) rng/Lvs.(5.68 ± 0.25) mg/L,(4.33 ± 0.12) mg/Lvs.(5.73 ± 0.31) mg/L,bothP＜0.001].The serum 25 (OH)D3 was not correlated with CRP or Gensini score in the normal group.In the ACS group,the serum 25 (OH) D3 was negatively correlated with CRP level and Gensini score (r =-0.026,P =0.045 ; r =-0.256,P =0.048),while CRP level was positively correlated with Gensini score (r =0.459,P ＜0.001).Inthe stable angina group,the serum 25 (OH)D3 was also negatively correlated with CRP level and Gensini score (r =-0.211,P =0.027; r =-0.208,P =0.029),and the latter two were positively correlated (r =0.574,P ＜ 0.001).Conclusions Low serum 25 (OH) D3 levels are associated with coronary artery stenosis.Combining with CRP,it may be involved in the pathogenesis of coronary heart disease through inflammatory mechanism.

BACKGROUND:Inflammatory cellactivation and the generation of oxygen free radicals are important factors of lung ischemia-reperfusion injury. Adding the drugs with anti-inflammation and antioxidant effects into the lung preservation solution used, can improve the protection fluid, and play a crucial role in the study of lung ischemia-reperfusion injury and protection of the function of transplanted lung. OBJECTIVE:To discussion the effect of harmine in canine model of pulmonary ischemia-reperfusion injury. METHODS:Twelve healthy hybrid dogs were randomly divided into two groups, with six rats in each group. A canine model of lung ischemia-reperfusion injury was established, and the protecting liquid was perfused with the clockwise irrigation method. Control group:low potassium dextran protective fluid;experimental group:low potassium dextran+harmine protective fluid. After 2 hours of ischemia, the left lung circulation was recovered. The left lung tissue and blood samples were col ected from two groups of animal models after reperfusion, and their cytokines levels and the lung wet/dry weight ratio were detected and calculated. Bronchoalveolar lavage fluid was col ected to observe the pathological indicators. The main pulmonary artery pressure, left and right pulmonary artery pressure were recorded by continuous monitoring. RESULTS AND CONCLUSION:There were no statistical y significant differences in the interleukin-17, tumor necrosis factorαand endothelin 1 content in the left lung tissue and the blood between the two groups at 2 and 4 hours after reperfusion (P>0.05). After 4 hours of reperfusion in both groups, the neutrophil number, the number of lymphocytes, alveolar edema index, and vascular wal damage in bronchoalveolar lavage fluid showed no statistical y significant differences (P>0.05). Through the analysis of variance, the main pulmonary artery pressure, left pulmonary artery pressure and right pulmonary artery pressure also had no statistical significance between the two groups (P>0.05). By the analysis of cytokines, pathological indicators, lung wet/dry weight ratio, and pulmonary arterial pressure, harmine has no significant lung protection effect in canine model of lung ischemia-reperfusion injury.

Objective To investigate the effects of glutathione protected gold nanoclusters (GSH-Au NCs) on HeLa cytotoxity.Methods Fluorescence intensity were measured on GSH-Au NCs containing medium treated cells using fluorescence spectrophotometer at different time points.GSH-Au NCs uptake by HeLa cells at 1,2,6,12 and 24 h were investigated through fluorescent spectrophotometer.In vivo tumor uptake was also investigated on BALB/c tumor-bearing mice through inductively coupled plasma mass spectrometry (ICP-MS) at 24 h after intraperitoneal injection of 0.2 ml GSH-Au NCs (3 mmol/L) and distilled water (control group) respectively.The cytotoxicity of GSH-Au NCs at different doses (0.003-0.3 mmol/L) was tested at 24 and 48 h using MTT assay after interaction with HeLa cells.Results The uptake efficiency of GSH-Au NCs by HeLa cells kept increasing and reached maximum of 73.13％ at 24 h.The results of tumor-bearing mice indicated that the tumor tissue had higher uptake efficiency after 24 h (320±15) ng/g than that of control group (intraperitoneal injection of distilled water),and the difference was stastically significant (P＜0.05).HeLa cells were treated with different concentrations of GSH-Au NCs for 24 h,and GSHAu NCs had a slight effect on cell viability.With the increase of GSH-Au NCs dose,the inhibition effects on growth of HeLa cells enhanced.The cell activity of HeLa cells treated with 0.3 mmol/L GSH-Au NCs for 24 h reduced to 86％compared with that of control group (the concentration of GSH-Au NCs was 0) (P＜0.05),while there was no significant difference between the survival rate of different concentrations of GSH-Au NCs group and the control group for 48 h.Conclusions GSH-Au NCs have neglectable cytotoxity on HeLa cells even though both in vitro and in vivo uptake are high.GSH-Au NCs are suitable for biomedical application such as imaging,drug loading and targeted drug delivery.

Object To compare the hupzine A (Hup A) in Huperzia serrata (Thunb.) Trev. obtained by different extracting methods and investigate the amount of alkaloids and the content of Hup A from different parts of the plants and from different places. Methods Using HPLC for the determination of Hup A. Results The content of Hup A in the stem and leaf is richer than that in the root. The content of Hup A from Guizhou, Guangdong and Anhui Provinces is 0.018%, 0.021% and 0.020% repectively; The difference of extract method of Hup A is no prominence. Conclusion The content of Hup A in the ground is richer than that of underground, and there are some difference in the content of Hup A obtained from different places.

Objective To study the two-phase flow dynamics distribution and red blood cell distribution under the fluid-solid coupling interaction in left coronary artery at the typical time point within one cardiac cycle,and to investigate the formation and development mechanisms of left coronary artery atherosclerotic plaque Methods The blood was regarded as a two-phase fluid.Based on fluid-solid interaction between blood and vascular wall,the computational fluid dynamics method was used to make the transient numerical simulation of two-phase flow in the left coronary artery under fluid-solid interaction;the distribution of blood flow in the left coronary artery at the typical time point within one cardiac cycle was studied,the relationship between hemodynamic parameters and the formation of atherosclerotic plaque was analyzed.Results A lowspeed eddy zone existed in an area between the distal segment of circumferential branch and the proximal outside of blunt-edge branch of the left coronary artery,where both internal wall shear stress and red blood cell volume fraction were very small and the blood flow pattern was very complicated.Conclusion At the lowspeed eddy zone that carries small wall shear stress,the lipid concentration polarization and macromolecular material deposition are easy to be produced.The area that has less red blood cells is liable to develop hypoxia,resulting in increased vascular wall permeability and intimal injury,which will activate the immune system,causing lipid accumulation in vascular wall and intimal hyperplasia and,thus,to induce the formation of atherosclerotic plaque.(J Intervent Radiol,2017,26:253-257)

Objective:To analyse the effects of mandibular swing approach in the treatment of the tumors in infratemporal fossa. Methods:11 patients with tumors in infratemporal fossa treated by the surgical operation with mandibular swing approach. The mandi-ble cut was at the front of foramina mentale in 5 cases, at the front of mandible angle in 4 cases and at the middle of chin in 2 cases re-spectively. Results:According to the nature, location, size and the relationship of the tumors with peripheral nerve and blood vessels, flexible selection of the position of mandibular cut could fully expose and completely remove the infratemporal fossa tumor. Conclusion:Surgical treatment of the tumors in infratemporal fossa by mandibular swing approach is safe and effective.

Kenaf has a high content of gum that is difficult to remove. Traditional chemical degumming process causes serious environmental pollution. To solve the problem, we developed a new method to degum kenaf. We pretreated the kenaf with steam explosion followed by ultrasonic treatment. We chose the single factor tests to select the ultrasonic frequency, sodium hydroxide concentration and processing time. Combined with orthogonal tests, we found that the optimum conditions were as follows: ultrasonic frequency was 28 kHz, sodium hydroxide concentration was 2%, and processing time was 60 min. Under these conditions, the residual gum of kenaf fiber was 9.72% and the fineness was 139.45 N(m). Steam explosion combined with ultrasonic method is effective in degumming of kenaf.
Hibiscus ; Plant Gums ; isolation & purification ; Sodium Hydroxide ; chemistry ; Steam ; Ultrasonics

Exopolysaccharide La0.1-1 was extracted from the broth of a marine bacterium Lentibacter algarum ZXM100T isolated from the seawater in the coastal region of Qingdao and purified by Q Sepharose Fast Flow ion-exchange chromatography and Superdex 75 gel-permeation chromatography. Its physiochemical properties and primary structural characters were investigated by chemical analysis together with high performance liquid chromatography (HPLC), high performance gel permeation chromatography (HPGPC) and gas chromatography and mass spectrometry (GC-MS). The results show that the total sugar content of the exoploysaccharide La0.1-1 was about 66% with an average molecular weight at 12.0 kDa. La0.1-1 is mainly composed of Gal, Man, GlcN at the ratio of 1.35:1.1:1.0. Results of GC-MS and NMR demonstrate that the exopolysaccharide La0.1-1 mainly exists with the beta configuration. The primary linkage styles are --> 2)-Manp(1 --> and --> 3)-Galp(1 --> with a small amount of --> 4)-Galp(--> 1 and --> 4)-Manp(1 --> linkages. The linkage mode of GlcN is --> 4)GlcN(1 --> and terminal linkage. The exopolysaccharide has mainly a linear sructure with a few branches linked to 0-6 of --> 2)-Manp(1 --> and 0-4 or 0-6 of --> 3)-Galp(1 -->. 1D-NMR data also revealed that La0.1-1 is substituted by certain acetyl; the acetyl is mainly linked to N-2 of GlcN. The exopolysaccharides of the bacterium of Lentibacter genus is reported for the first time, and an exopolysaccharide with novel structure was obtained, which enriched marine polysaccharide resources.
Chromatography, Gel ; Chromatography, High Pressure Liquid ; Chromatography, Ion Exchange ; Gas Chromatography-Mass Spectrometry ; Magnetic Resonance Spectroscopy ; Molecular Weight ; Polysaccharides ; chemistry ; isolation & purification ; Rhodobacteraceae ; chemistry ; Seawater ; microbiology

Background and purpose:Cytotoxic T lymphocyte (CTL) plays a vital role in the process of anti-tumor immunology. The aim of this study was to investigate whether changes in concentration of IL-2 (50, 200 and 1 000 U/mL) would affect the sub-population and cytotoxic function of cells cultivated by peptide-specific CTL induction systemin vitro and also observe whether using the concentration of IL-2 at a range of 50-1 000 U/mL isbeneifcial to regulatory cells (Tregs) enrichment.Methods:Peripheral blood from 10 healthy donors and 10 cancer patients that were HLA-A2 positive, were collected in the study. HLA-A2 restricted CTL epitope P321 (ILIGETIKI) derived from COX-2 pulsed with different concentrations of IL-2 were used to induce peptides-speciifc CTLin vitro. Flow cytometry was performed to analyze the proliferative capability, the proportion of different T-cell subsets, and secretion of perforin, granzyme B and IFN-γ. IFN-γ secretion was assessed by ELISpot assay.Results:High concentration of IL-2 increased the proliferative activity. The percentage of CD4+ T cells of cancer patient group was signiifcantly higher than that of healthy donor group, while the percentage of CD8+ T cells of cancer patient group was signiifcantly lower than that of healthy donor group. And there was no signiifcant difference in the percentages of CD4+ T cells, CD8+ T cells and Tregs among groups with different IL-2 concentrations. No difference was seen in cytokine (perforin, granzyme B, IFN-γ) secretion capacity of CD8+ T cells. ELISpot study revealed that high-dose IL-2 resulted in the increasing of IFN-γ secretion.Conclusion:The sub-population and the function of cells cultured by peptide-speciifc CTL induction systemin vitro are not affected by different concentrations of IL-2. Furthermore, high concentrations of IL-2 (50-1 000 U/mL) do not provide the enrichment for Tregs. Higher concentration of IL-2 is likely to cause high secretion of IFN-γ in ELISpot assay. In order to exclude the distraction of NK cells or NKT cells, the concentration of 50 U/mL is better choice.