1.Intraoral approach for abrasive mandibular reduction osteoplasty
Yuanlong ZHANG ; Xincan WANG ; Ningxin CHENG
Chinese Journal of Medical Aesthetics and Cosmetology 2009;15(5):294-296
Objective To verify the clinical reliability of a simple abrasive method for reduction mandibular osteoplasty.Methods A total of 598 cases of reduction mandibular osteoplasty were performed by using the abrasive method since 2002.Through intraoral incisions,the low part of mandibular ramus,angle region,buucal cortice and inferior border of mandible were exposed and abrasive osteoplasty was taken with special designed retractors,rasp and grinding burs with protecting sheath.The inferior alveolar nerve could be preserved.Results There was no nerve injury and only one hemorrhage case during operation without unfavorable result.In 6 to 1~2 months follow-up for 168 cases,the satisfactory rate for contour results was 95.24%.Conclusion Through the intraoral approach,the abrasive mandibular reduction osteoplasty is a simple,safe and effective method for mandibular osteoplasty.It is not to correct the prominent mandibular angle but to reduce the thickness of the mandibular ramus and body.This method could contribute the same results of angle ostectomy and split cortical bone ostectomy and result a good contour of the jaw region.
2.Photorejuvenation for face telangiectasias by intense pulsed light: a preliminar y clinical report
Qi LUO ; Yuanlong ZHANG ; Minggen XIONG ; Al ET
Chinese Journal of Medical Aesthetics and Cosmetology 2001;0(05):-
Objective To explore the clinical efficacy of photoreju ve nation in the treatment of face telangiectasias by intense pulsed light. Methods A total of 56 patients (age ranged from 27~39 years ) wh o suffered from telangiectasias were cured in this hospital since November 2001. Results All the cases were divided into three clinical grou ps: mild, intermediate and severe. After the treatment of 4 to 8 times, 17 patie nts were cured (30.4 %), 23 patients with significant improvement (41.0 %), 13 p atients with improvement (23.3 %), and no effect in 3 cases (5.3 %). Co nclusions Intense pulsed light is an excellent new technique for f ace telangiectasias. Correct operation parameters are important factors for sati sfactory clinical efficacy.
3.Relationship between gut mucosal barrier dysfunction and immune response in patients with early severe acute pancreatitis
Jun YANG ; Yuanlong GU ; Donglin JIANG ; Feng ZHANG ; Yi QIAN ; Jianping LI
Chinese Journal of Hepatobiliary Surgery 2014;20(4):269-273
Objective To explore the relationship between gut mucosal barrier dysfunction and immune response in patients with early severe acute pancreatitis (SAP).Method 46 patients with early SAP were enrolled into this study from January 2010 to December 2012.Results The levels of endotoxin,the lactulose/mannitol (L/M) ratio,the D (-)-lactate concentration,and the proportion of HLA-DR-positive monocytes all decreased from a high level,while the frequency of Tregs increased during the first 14 days.The Th1/Th2 ratio decreased,with a decreased Th1 and an increased Th2 profile in the first week,but it subsequently increased,with an increased Th1 profile.Conclusions The data from this study showed that immunosuppression and immune imbalance happened in patients with early SAP,and showed a positive relationship with gut mucosal barrier dysfunction in patients with early SAP.Thus,early protection of the function of intestinal mucosal barrier is important in the treatment of SAP.
4.Measurement of the femoral neck torsion angle and anteversion angle by laser projection method
Qiuliang ZHU ; Bing XU ; Lianghua SHEN ; Lilai ZHAO ; Maohua YAN ; Yuanlong WANG ; Ying ZHANG
Acta Anatomica Sinica 2014;(5):694-697
Objective To investigate the feasibility of measuring femoral neck torsion angle and anteversion angle by laser projection method .Methods The femoral neck torsion angle and anteversion were observed and described .An angle measuring device was designed and produced .With the device , the femoral torsion angle and anteversion angle were measured by laser projection method two times .Statistical analysis was performed on the measured value , and sides difference .Results The differences between femoral neck torsion angle and anteversion angle were observed .There was no significant difference ( P >0.05, power =100%) between the two measurements by laser projection method . Measurements of the femoral anteversion were 13.58 °±6.55 °on the left side , and 12.15 °±5.83 °on the right side . Measurements of the femoral neck torsion angle were 18.50 °±7.38 °on the left and 19.08 °±8.59 °on the right .There was no significant difference between left and right side ( P >0.05 ) .Conclusion The laser projection method is the effective method in measuring femoral neck torsion angle and anteversion angle , and has excellent repeatability .
5.Primary cultivation and identification of human placental microvascular endothelial cells
Huili ZHANG ; Peili DU ; Yuanlong FANG ; Jing ZHANG ; Yutian HE ; Bin SUN ; Xue XIAO ; Wen SUN ; Yanmei ZHOU ; Dunjin CHEN
Chinese Journal of Tissue Engineering Research 2014;(11):1706-1711
BACKGROUND:Establishment of in vitro culture system of human placental microvascular endothelial cel s with high purity is very important. In recent studies, some scholars have successful y obtained a large number of placental microvascular endothelial cel s by three-stepenzyme digestion and magnetic separation method, but the procedures were extremely complex and it had great damage to the cel s. Therefore, how to separate human placental microvascular endothelial cel s easily and obtain high-purified cel s has become a research hotspot.
OBJECTIVE:To investigate an efficient method to isolate and purify human placental microvascular endothelial cel s from early vil us microvessels, observe the cel growth and identify the cel s.
METHODS:The vil i from normal early pregnancies (6-8 weeks) after artificial abortion were col ected aseptical y. Using two-step digestion procedure and discontinuous Percol density gradient centrifugation method, human placental microvascular endothelial cel s were obtained. Then the cel s were identified by trypsin digestion method and repeated adherence method.
RESULTS AND CONCLUSION:Human placental microvascular endothelial cel s were isolated successful y from early vil i. The primary cel s adhered to the wal s after inoculated for 24 hours and entered logarithmic phase at 10 days. 80%of the cel s achieved a confluence at 12-13 days after inoculating. The subculture cel s grew swiftly with the typical cobblestone appearance. Immunofluorescence staining showed that, cultured human placental microvascular endothelial cel s demonstrated a strong positive reaction to von Wil ebrand factor antigen and CD31, accounting for 100%. MTT assay results showed that, human placental microvascular endothelial cel s at passage 5 exhibited an S-shaped growth curve. High-purity human placental microvascular endothelial cel s can be obtained by proteolytic enzymes digestion and discontinuous Percol density gradient centrifugation method, and the purity is detected by trypsin digestion method and repeated adherence method.
6.Evaluation and continuous improvement of the application of critical values
Haili LAN ; Xiuming ZHANG ; Yuanlong YU ; Yauye YANG ; Yong YANG ; Zhian HAN ; Yuyan LI ; Nengliang OUYANG ; Hongxiang XIE
Chinese Journal of Hospital Administration 2009;25(4):235-238
Objective To discover regularities behind critical values in an effort to provide scientific evidences for a better critical value report system.Methods Collection, analysis and statistical assessments for critical value items and ranges appropriate for the hospital Results The occurrence rate of critical values is found to be 0.14%.By means of clinical evaluation, statistic analysis and reference to literature and clinical specialists, the clinical labs revised the ranges of PO2, GLU, PT and APTT, and added ALT, MYO, CTnl and K+ , GLU, BILl for newborns, and canceled AMY.All of these changes were put in practice upon approval of the Medical Department of the hospital Conclusion Regular evaluation and continuous improvement of the critical value report system may help with saving lives, improving quality of care of the labs and doctors" diagnosis as well
7.Research progress of animal model of iatrogenic benign biliary stricture
Shuo ZHANG ; Yuanlong GU ; Wanqing GU
Chinese Journal of Hepatobiliary Surgery 2020;26(10):797-800
With the popularity and development of minimally invasive surgery such as laparoscopic cholecystectomy, the incidence of iatrogenic biliary tract injury and biliary duct stenosis after injury has been increased significantly. The biliary duct injury of laparoscopic cholecystectomy is mainly heat damage, and the biliary duct tissue is prone to biliary duct stenosis after heat injury. Currently the treatment of complex iatrogenic biliary stricture has still been a difficult and unfinished problem. It’s necessary to explore the therapy options of iatrogenic biliary stricture based on animal models of biliary stricture. According to the literature at home and abroad, this article reviewed the methods and advantages and disadvantages of several models of benign biliary stricture.
8.Construction of Hsf1 gene-knockout mouse model using CRISPR/Cas9
Zheyu WU ; Zhenhua ZHOU ; Zhipeng WU ; Yufeng ZHANG ; Zhiqiang YANG ; Yufan ZHU ; Yuanlong XIE ; Jianru XIAO ; Lin CAI
Chinese Journal of Orthopaedics 2021;41(8):515-524
Objective:To explore the efficient construction of HSF1 gene knockout mouse model using CRISPR/Cas9 gene editing technology, and to establish the early basis for the mouse model of primary osteosarcoma.Methods:According to exon 9 of HSF1 gene structure, the corresponding GRNA (guideRNA) was selected and screened. Then the transcription template of sgRNA (small guide RNA) was amplified by PCR, and four up stream primers were obtained. Subsequently, sgRNA was transcribed in vitro and screened by Tube Screen platform to screen the sgRNA with effective cutting, and the sgRNA with the highest cutting efficiency was selected from the screening results for subsequent experiments. The transcription template of SPCas9mRNA was amplified by PCR, and then Cas9mRNA was transcribed in vitro. The sgRNA transcribed in vitro and Cas9mRNA were injected into the fertilized eggs of healthy C57BL/6 mice, and the tissue was extracted from the tail of the born mice and identified by PCR sequencing. Heterozygous female mice of F0 generation were selected to mate with wild-type male mice too btain F1 generation off spring. The mutation of gene bases of F1 generation mice was detected by AGAR gel electrophoresis and gene sequencing. The heterozygous male mice of the F1 generation and female mice of the F0 generation were back crossed to obtain the F2 generation daughter mice. The tail tissues were cut and sequenced to obtain the F2 generation homozygous knockout mice. PCR was used to observe the cutting efficiency of sgRNA and the sequencing of rat tail tissue, and SNAPGene software was used for gene sequence alignment to determine the deletion of base fragments.Results:The up stream primers sgRNA-1 Primer-f, sgRNA-2 Primer-f, sgRNA-3 Primer-f, sgRNA-4 Primer-f and down stream primers sgRNA-4 Primer -r were obtained by PCR amplification. After in vitro tran scription and screening of sgrRNA, sgrRNA-1, sgrRNA-2 and sgrRNA-4 had high cleavage efficiency and were selected for subsequent experiments. T7 promoter was added to the 5 'end of Cas9 mRNA, and Cas9 mRNA was obtained by PCR and in vitro transcription kit. Mixed Cas9-sgRNA solution was injected into the fertilized eggs of mice and cultured. The cultured two-cell fertilized eggs were injected into the ampulla of the pseudo pregnant female mice, and the F0 generation mice were obtained successfully. A total of 8 heterozygous mice of F0 generation were obtained by Agar gel electrophoresis. Three heterozygous knockout mice of F1 generation were obtained by breeding the female heterozygous mice of F0 generation with healthy wild-type male mice and PCR and sequencing. Three heterozygous male mice of F1 generation were back crossed with female mice of F0 generation 3 to obtain F2 generation mice. Through the observation of electrophoresis and sequencing results of F2 generation mice, it was confirmed that 7 mice were missing HSF1 base sequence, and the electrophoresis results showed mutant bands and no wild-type bands, which were identified as homozygous. The F2 generation homozygous mice were able to breed stably. As eries of results proved that the HSF1 gene knockout mouse model was successfully established in this experiment.Conclusion:CRISPR/Cas9 technology was successfully used to construct HSF1 gene knockout mouse model, with strong stability and high reproducibility, which laida foundation for further study of HSF1 gene expression products and establishment of mouse model of primary osteosarcoma.
9.Significance of Preoperative Nerve Reconstruction Using Diffusion Tensor Imaging Tractography for Facial Nerve Protection in Vestibular Schwannoma
Yuanlong ZHANG ; Hongliang GE ; Mingxia XU ; Wenzhong MEI
Journal of Korean Neurosurgical Society 2023;66(2):183-189
Objective:
: The facial nerve trace on the ipsilateral side of the vestibular schwannoma was reconstructed by diffusion tensor imaging tractography to identify the adjacent relationship between the facial nerve and the tumor, and to improve the level of intraoperative facial nerve protection.
Methods:
: The clinical data of 30 cases of unilateral vestibular schwannoma who underwent tumor resection via retrosigmoid approach were collected between January 2019 and December 2020. All cases underwent magnetic resonance imaging examination before operation. Diffusion tensor imaging and anatomical images were used to reconstruct the facial nerve track of the affected side, so as to predict the course of the nerve and its adjacent relationship with the tumor, to compare the actual trace of the facial nerve during operation, verify the degree of coincidence, and evaluate the nerve function (House-Brackmann grade) after surgery.
Results:
: The facial nerve of 27 out of 30 cases could be displayed by diffusion tensor imaging tractography, and the tracking rate was 90% (27/30). The intraoperative locations of facial nerve shown in 25 cases were consistent with the preoperative reconstruction results. The coincidence rate was 92.6% (25/27). The facial nerves were located on the anterior middle part of the tumor in 14 cases, anterior upper part in eight cases, anterior lower part in seven cases, and superior polar in one case. Intraoperative facial nerve anatomy was preserved in 30 cases. Among the 30 patients, total resection was performed in 28 cases and subtotal resection in two cases. The facial nerve function was evaluated 2 weeks after operation, and the results showed grade I in 12 cases, grade II in 16 cases and grade III in two cases.
Conclusion
: Preoperative diffusion tensor imaging tractography can clearly show the trajectory and adjacent position of the facial nerve on the side of vestibular schwannoma, which is beneficial to accurately identify and effectively protect the facial nerve during the operation, and is worthy of clinical application and promotion.
10.Mechanisms of Depressor Effect of Norepinephrine Injected into Subnucleus Commissuriu of Nucleus Solitarius Tractus in Rabbits
Yi ZHANG ; Hongyan LUO ; Shenghong LIU ; Zhengrong YI ; Ai LI ; Xinwu HU ; Changjin LIU ; Ming TANG ; Lieju LIU ; Yuanlong SONG ; Linlin GAO
Journal of Huazhong University of Science and Technology (Medical Sciences) 2005;25(3):263-264,268
Summary: This experiment aimed to investigate the effect of adrenergic system in the subnucleus commissuriu of nucleus solitrius tractus (CNTS) on renal nerve discharges. Norepinephrine (NE) was microinjected into the CNTS of rabbits and mean arterial blood pressure (MAP) and renal nerve discharges (FRND) were synchronously recorded. The results indicated that (1) microinjection of norepinephine into the CNTS of rabbit could significantly attenuate the frequency of renal nerve discharge, and at the same time decrease markedly the mean arterial pressure. (2) Microinjection of 0.3 nmol yohimbin into CNTS had no significant influence on FRND and MAP, but could attenuate and even reverse the effects of NE on FRND and MAP. These results suggest that microinjection of NE into CNTS may activate the alpha-adrenorecptor located in CNTS and secondarily produce a depressor effect by attenuating the activity of peripheral sympathetic nervous system.