Objective:To discuss the accounting method for clinical usage efficiency of free medical consumable under information management system, and analyze these usage efficiency data.Methods: To adopt the medical material management system to collect usage quantity and amount of free medical consumables and chargeable medical consumable, respectively, from different department in every month, and then generalize usage quantity of different consumable; to apply hospital information system (HIS) to collect monthly patient data of different disease area or department; to gather statistics of monthly data proportion of usage ratio of free medical consumable as classified statistic principle by applying the consumption of them, patient quantity and usage proportion depend on data from two systems; and then got the line analysis charts of monthly usage efficiency and average usage efficiency of free medical consumable of each department in one year.Results: The data were evaluated through usage rate of free medical consumable of each department; the reference data from direct data and graph analysis depended on different classification and time interval, and they were provided for clinic practice in order to the free medical consumable can be more reasonable used in guiding clinical department.Conclusion: The purchase and used quantity of medical consumable can be grasped through information management system, which is scientific in management and usage for them, can avoid excessive waste and reduction of medical consumable, ensure them can be safety and efficiency used in clinical diagnosis and treatment. It make medical materials to be used in clinical diagnosis safely and effectively.

Objective To investigate the expression changes of microRNAs and VEGF-NOTCH in renal ischemic injury in mice, and to explore the potential mechanism associated with renal angiogenesis.Method Male Balb/c mice were subjected to a standard renal ischemia to induce acute kidney injury (AKI) after 45 min of bilateral renal artery clamping. Following 4 h, 24 h of reperfusion or sham operation, kindey tissues were collected and subjected to detect the expression changes of microRNAs which relatived with angiogenesis and VEGF, Flk-1, Notch1 mRNA by Quantitative Real-time RT-PCR. Flk-1 protein was detected by Western blotting analysis at 24 h and 72 h following Ischemia/Reperfusion(I/R) injury. The expression of CD31 was examined in tissue sections by immunohistochemistry staining, and the microvessels in ischemic region of each group were counted. Results miRNA-210 and miRNA-92a expression increased significantly, with prominent changes at 4 h and 24 h after reperfusion( P ＜ 0.05 ). VEGF and Flk-1 mRNA expression and Flk-1 protein were increased in renal I/R compared with control group respectively (P＜0.05 ).Immunohistochemistry staining results of CD31 showed a significant increase of microvessels in renal ischemic region. Conclusion This study first reported the changes in miRNAs expression in response to kidney I/R in mouse. our results implied that miRNAs may be involved in targeting VEGF-Notch pathway signaling to regulate angiogenesis after renal I/R injury. It provided novel insights into the angiogenesis mechanism of renal ischemic injury.

Objective:To analyze the differential expression profile of circRNA and the expression changes of Hippo signaling molecule YAP in renal ischemia-reperfusion injury of mice.Methods:A model of renal IR damage in mice was induced, and serum creatinine (Scr) and urea nitrogen (BUN) concentrations and histological changes of samples were detected to assess renal function and tubular injury. Illumina HiSeq 2500 system was used for high-throughput paired-end sequencing to establish the circRNA expression profile with significant differential expression. Real-time quantitative polymerase chain reaction (qRT-PCR) verified the sequencing results and detected related genes. Gene function (GO) and pathway (KEGG) analysis were performed to predict the biological processes and the major signal pathways involved by differentially expressed circRNAs. The expression level of the main signaling molecule was examined by western blot.Results:Twenty-one distinctly differentially expressed circRNAs ( fold change ≥ 2) were found in IR 24 h kidney tissues compared with the expression in the control groups ( P < 0.05), among which 10 circRNAs were observed to be up-regulated and 11 down-regulated. CircRNA.1100 and circRNA.1122 were randomly (random number) selected for verification by qRT-PCR, and the relative expressions after renal IR 1day were decreased by (0.23±0.016) and (0.36±0.12), respectively, which were highly consistent with the sequencing trends. Analysis of biological functions and pathways showed that differential expression circRNA was significantly enriched in cell cycles, division, growth, apoptosis, death, and Hippo signaling pathways. The Hippo pathway effector molecule YAP protein was significantly up-regulated after renal IR 1day and until the 3rd day of IR. Conclusions:CircRNA may be involved in the regulation of renal IR injury. CircRNA and Hippo pathway may play a key role in the development of renal IR injury.

Objective:To investigate the effect of extracellular vesicles derived from lung tissue on intrapulmonary inflammation and the formation of neutrophil extracellular traps (NETs) in sepsis rats.Methods:Sepsis rat model was established by cecal ligation and puncture (CLP). Collagenase D and DNase I were used to dissociate the lung tissue, the impurities were removed by centrifugation, and finally, the extracellular vesicles (Ti-EVs) derived from lung tissue were separated and extracted by differential ultracentrifugation. Eighteen male SD rats were randomly divided into the sham group, sepsis group and Ti-EVs group: in the Ti-sEV group, a sepsis model was established by CLP, and Ti-EVs suspension was instilled through the airway; rats in the CLP group received CLP, and an equal volume of PBS was instilled through the airway; and rats in the sham group was treated with sham operation. The pathological changes of lung tissue were detected by hematoxylin-eosin (HE) staining after 24 h. The content of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the bronchoalveolar lavage fluid (BALF) was measured by enzyme-linked immunosorbent assay (ELISA). Immunofluorescence was used to detect the NETs content in lung tissue.Results:The isolated extracellular vesicles derived from rat lung tissue were observed by transmission electron microscopy as double-layer circular cystic vesicles with particle diameter mainly distributed at 150 nm. Western blot showed positive expression of EVs markers CD9, CD63, and Tsg101. HE staining of lung tissue showed alveolar integrity damage and a large number of inflammatory cells infiltrated in the lung of sepsis rats. Compared with the CLP group, the degree of lung tissue damage was more serious in the Ti-EVs group and the levels of IL-1β, TNF-α and IL-6 in the BALF of rats were significantly increased ( P<0.01). The formation of NETs in the lungs of the rats in the sepsis group and the Ti-EVs group was observed under the laser confocal microscope. Compared with the sepsis group, the fluorescence intensity of NETs in the lung tissues of the Ti-EVs group increased significantly. Conclusions:After enzymatic digestion, differential ultracentrifugation and other treatments, the extracellular vesicles derived from rat lung tissue with high purity can be successfully isolated and extracted. In the process of septic lung injury, extracellular vesicles in lung tissue can aggravate the inflammatory response in the lung and promote the formation of NETs.