Objective To study liver function, hepatic energy metabolism, regeneration and apoptosis in obstructive jaundiced or normal liver after 70% partial hepatectomy (PH) with hepatic artery resection (HAR) in rats. Methods In this study, 133 male SD rats were enrolled, 6 rats were in sham operation group, 20 rats underwent choledochoduodenostomy after 70% PH and 20 rats did 70%- PH, choledochoduodenostomy plus HAR. The remaining 87 rats 5 days after common bile duct ligation (CBDL) were randomized into two groups: 70% PH with choledochoduodenostomy, and 70% PH with choledochoduodenustomy plus HAR. Serum TB, ALT, ALB and ALP; tissue of hepatic HGF, bcl-2 mRNA and protein expression; ATP, ADP and AMP in hepatic tissues; hepatocyte proliferation/ apoptosis index were observed postoperatively (24 h, 72 h and 7 d). MortaLity was calculated. Results Rats without obstructive jaundice could tolerate 70% PH plus HAR with good liver regeneration. Compared with other groups, the serum liver function index; ATP content and EC value; HGF,bcl-2 mRNA content of liver tissue and the hepatocyte proliferation/apoptosis index in 70% PH with HAR group significantly aggravated and the mortality signiticanfly increased in obstructive jaundice rats ( P < 0. 05). Conclusions (1) The liver regeneration and apoptosis were not significantly influenced in normal mrs undergoing 70% PH and 70% PH with HAR, moreover hepatoeyte energy metabolism and liver function recovered rapidly in both groups. (2) With the existence of severe bilirubinemia, 70% PH with HAR caused an increased mortality suggesting a rationale for a preoperative bilirubin reducing procedures before a major surgery in malignant obstructive jaundice.

Objective To compare autologous stem cell transplantation and insulin action glycemic control in patients with type 1 diabetes. Methods A total of 40 patients with newly diagnosed type 1 diabetes patients were divided into transplantation group of 15 patients and 25 cases of insulin groups accorded to treatment, transplantation group treated with autologous hematopoietic stem cell transplantation, insulin group with conventional insulin injection therapy, fol-low-up of > 12 months, 12 months after treatment the adoption of continuous glucose monitoring and evaluation of glycemic control was evaluated. Results Transplantation group after treatment, MBG, LAGE were significantly lower than before treatment (P<0.05), after treatment, transplantation group MBG, SDBG, LAGE, MAGE were significantly lower than insulin group (P<0.05). After the transplant group therapy significantly reduced HbA1c compared with be-fore treatment and significantly lower than in the insulin group(P<0.05), group 2 patients transplanted insulin were de-activated in 9.3,11.2 months, an average reduction of insulin (53.28±21.29)%, after treatment, the amount of insulin significantly reduced transplant group and significantly lower than the insulin group (P<0.05), post-transplant group therapy blood glucose <3.9 mmol/L percentage of time, blood glucose > 11.1 mmol/L percentage of time were signifi-cantly lower than before treatment, and significantly lower than insulin, glucose (3.9~11.1) mmol/L percentage of time increase in the percentage of time now compared to before treatment, and significantly higher than that in the insulin group (P<0.05). All patients with severe adverse reactions occur. Conclusion Compared to conventional insulin injec-tion therapy, autologous hematopoietic stem cell transplantation can effectively control blood sugar with type 1 diabetes, can reduce blood sugar fluctuations, reduce insulin dependence.

Cases of acute pancreatitis induced by organophosphate intoxication are encountered occasionally in clinics, but very few of them develop into severe pancreas necrosis and irreversible pancreatic function impairment. Here, we report a 47-year-old female organophosphate poisoning case after ingestion of massive insecticides; she was considered to have total necrosis and function failure of the pancreas via serum amylase test, glucose level test, and CT imaging. The patient exhibited no relief under the regular medicine treatment, which included sandostatin, antibiotics, intravenous atropine, and pralidoxime methiodide. She received percutaneous catheterization and drainage of pancreatic zone to expel hazardous necrotic waste, also by which the pathogenic evidence was obtained and the antibiotics were adjusted subsequently. The patient recovered gradually, was discharged after 2 weeks, and was prescribed with oral pancreatin capsules before meals and hypodermic insulin at meals and bedtime to compensate the impaired pancreatic function.
Acute Disease ; Anti-Bacterial Agents ; therapeutic use ; Catheterization ; Female ; Humans ; Insecticides ; poisoning ; Middle Aged ; Organophosphate Poisoning ; Pancreas ; diagnostic imaging ; pathology ; Pancreatin ; therapeutic use ; Pancreatitis ; chemically induced ; diagnostic imaging ; therapy ; Treatment Outcome