Objective To compare the serum protein profiles between people with successful aged 80 years and over in Bama versus Nanning,to identify the possible causes of the long life expectancy in Bama.Methods We evaluated the serum protein profiles using surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) with 4 different chromatographic surfaces,including the weak cation exchange (CM10),immobilized metal affinity capture (IMAC) 30-Cu,strong anion exchange (Q10) and hydrophobic surface (H50) protein chip arrays in people with successful aged 80 years and over living in Bama versus in Nanning city.SELDI-TOF-MS had a molecular mass detection range of 2000-50,000 D,with a first signal-to noise (S/N) ratio of 5,frequency of peak cluster of over 15 ％,and second S/N ratio of 2 and peak fluctuation of 0.3％ (m/z).Results The total numbers of serum protein (peptide) peaks for people with successful aging in Bama and Nanning were 71 on CM10,46 on IMAC30-Cu,81 on Q10,and 52 on H50.Among subjects from Bama as compared with Nanning (P＜0.05; mean ＞SD for each protein peak),there were 8 differently expressed protein peaks on CM10,of which 2 were upregulated and 6 downregulated; 7 on IMAC-Cu,of which 2 were upregulated and 6 downregulated; 5 on Q10,all of which were upregulated; and 6 on H50,of which 3 were upregulated and 3 downregulated.Conclusions There are marked differences in the serum protein profiles between people with successful aging living in Bama versus in Nanning.Most of the proteins are downregulated among people aged over 80 years in Bama.

Objective To investigate the effect of vertical bed training on the extubation time and pulmonary infection of patients with tracheotomy in rehabilitation department .Methods Sixty-five new patients with tracheotomy and pulmonary infection more than a month were randomly divided into the control group (33 cases) and the observation group (32 cases) by stratified block randomization .All the patients received routine nursing and rehabilitation therapy , in addition, the patients in the observation group received electrodynamic vertical bed training .The decannulation rate , cure rate of pulmonary infection , sputum culture negative rate and the clinical pulmonary infection scale ( CPIS) were compared.Results One patient was lost in each of the groups during follow up .After the nursing intervention , the CPIS of the observation group was significantly lower than that in the control group (t=9.276,6.609, respectively;P<0.01).22 patients (71.0%) in the control group removed the tracheal tube;20 patients (64.5%) cured of pulmonary infection;15 patients (48.4%) had negative sputum culture;the sare of CPIS was (2.8 ±1.1), those values of the control group were 43.8%, 37.5%, 25.0%and (3.9 ±1.5).There were significant differences between groups ( t/χ2 =4.76,4.60, 6.22,3.36, respectively;P<0.05).Conclusions Vertical bed training can reduce the time of tracheostomy , and help in control the pulmonary infection of patients with tracheotomy in rehabilitation department .

The protein production system using a baculovirus Antheraea pernyi nucleopolyhedrovirus (AnpeNPV) as a gene expression vector and its host insect as a natural bioreactor was successful established and its excellent performance in the protein production has been demonstrated. In this paper, the system is used to produce recombinant human epidermal growth factor (rhEGF), which have been widely used in medical and cosmetic treatment. A recombinant AnpehEGF virus has been constructed by replacing the viral polyhedrin gene with the rhEGF gene, and then injected it to Samia cynthia ricini pupae. Amplification and expression of rhEGF gene in the pupae was clearly detected by PCR, Western blot and ELISA analyses. These analyses have also revealed that rhEGF in the pupae was significantly increased at 6 days post-infection, and reached maximum level at the 12th day. The concentrations of rhEGF were 19.77, 24.90, 618.59 and 1 952.46 ng/g pupae at 3, 6, 9 and 12 days post-infection, respectively. However, the rhEGF concentration reduced at later stage (days 15). The rhEGF in the pupae could be purified using ammonium sulfate precipitation and Ni-NTA agrose affinity chromatography. Results demonstrate that Samia cynthia ricini pupae can be used as a bioreactor to produce rhEGF and, if successfully improved, will be a novel method of rhEGF production with lower cost and more efficient.
Amino Acid Sequence ; Animals ; Bombyx ; genetics ; metabolism ; Epidermal Growth Factor ; biosynthesis ; genetics ; Genetic Vectors ; genetics ; Humans ; Molecular Sequence Data ; Nucleopolyhedrovirus ; genetics ; Recombinant Proteins ; biosynthesis ; genetics

Objective To investigate the influencing factors of using active imagery therapy in spinal cord injury patients with neurogenic bladder. Methods Ninety-two spinal cord injury patients were arranged for bladder function training by active imagery therapy. According to the training effect, they were divided into two groups, and a total of eleven factors were observed including age, education level, time of rehabilitation intervention, the type of spinal cord injury and etc. The logistic regression was used to analyze the possible influence factors of the two groups. Results The analysis results indicated that there were significant differences in education level, depression state, the type of neurogenic bladder, rehabilitation time and course of treatment which were affect the bladder training (χ2 =5. 851, 6. 903, 6. 901, 30. 597, 4. 211,respectively;P<0. 05). The results showed that below the junior college level, HAMD≥17, time of rehabilitation intervention≥60 d, treatment course <8 weeks, incontinence of urine were the adverse factors. Conclusions Education level, depressive state, time of rehabilitation intervention, treatment course, type of neurogenic bladder were the prognostic factors in spinal cord injury patients who was arranged for bladder function training by active imagery therapy. Aiming at these factors, medical staff should provide necessary guidance to improve the results of treatment.

Objective:To summarize the clinical features and prognosis of acute kidney injury in patients with HBV related acute on chronic liver failure (ACLF).Methods:A total of 150 patients who developed acute kidney injury (AKI) in patients with HBV related ACLF from Sep. 2010 to Sep. 2019 were reviewed retrospectively, and the gender, age, laboratory examination, Child-pugh scores, and model for end-stage liver disease (MELD) were collected and the survival of the patients were followed up to analyze the prognosis.Results:Ninety-three percent of the patients were complicated with ascites, 81% with spontaneous peritonitis, 65% with hepatic encephalopathy and 58.7% with pulmonary infection; 60 patients (60.0%) were AKI stage 1, 44 patients (29.3%) were AKI stage 2, 16 patients (10.7%) were AKI stage 3. The patients with hyponatremia had lower albumin ( t=2.571, P=0.011), higher blood urea nitrogen, serum potassium and white blood cell levels than those without hyponatremia ( t=3.184, P=0.002; t=2.069, P=0.040; t=2.251, P=0.026); 74.7% of the patients died within 30 days, and the 90 days survival rate was 16.7%. The 30 days and 90 days mortality of patients with hyponatremia was higher than that of patients without hyponatremia ( χ2=4.11, P=0.044; χ2=3.901, P=0.049 7). Kaplan-Meier analysis revealed that the patients who had abnormal uric acid pre-diagnosis of AKI, hyponatremia when diagnosis of AKI, organ damage other than liver and kidney, metabolic acidosis, upper gastrointestinal tract bleeding, hepatic encephalopathy had a poor survival. Cox regression analysis showed that other organ function damage other than liver and kidney, metabolic acidosis, and the old age, were independent risk factors of death. Conclusions:Most of the AKI patients with HBV related ACLF had ascites and spontaneous bacterial peritonitis when AKI occurred, and AKI stage 1 was common. The mortality of patients with hyponatremia was high, and the risk of death was high in patients with severe organ damage other than liver and kidney, metabolic acidosis and the old age.