1.Clinical study on 45 cases with endogenous caesarean scar pregnancy
Chinese Journal of Primary Medicine and Pharmacy 2015;(21):3283-3285
Objective To study the effect of ultrasound guided curettage after methotrexate and mifepristone in the treatment of endogenous caesarean scar pregnancy.Methods A retrospective analysis was performed on 43 patients of endogenous caesarean scar pregnancy undergoing treatment of ultrasound guided curettage after methotrexate and mifepristone.Results 39 cases were successful,4 cases were transformed to laparotomy because of intraoperative blood loss,7 cases of bleeding after curettage were successful by uterine carity placed double lumina Foley catheter. Conclusion Ultrasound guided curettage after methotrexate and mifepristone is a safe,effective,little trauma and low cost method in the treatment of endogenous caesarean scar pregnancy,it especially adapts to primary hospital.
2.Study on the Informed Consent Right of Patients with Mental Disorders
Chinese Medical Ethics 2016;29(4):690-693
Mental Health Law has specify the mental disorder patients’ informed consent right,but in practice, the problems,the mental health status of patients, namely the families’ right of subrogation exercise,“loss of self-control or deny having mental disorder”, have prevented the exercising of informed consent right. Therefore,it is necessary to effectively solve this plight of rebuilding a harmonious relationship between doctors and patients,estab-lishing mentally disordered patients’ right to advance directives, especially choosing the instrument of assessing the individual’ s capacity to consent.
3.Interferon gamma enhances the cytotoxicity of 5′-deoxy-5-fluorouridine and 5-fluorouracil against human bladder cancer cells
Gang LI ; Yuanfang ZHANG ; Qiang DING
Chinese Journal of Urology 2001;0(07):-
Objective To investigate the regulative effect of interferon gamma (INF?) on the expression of platelet-derived endothelial cell growth factor / thymidine phosphorylase (PD-ECGF/TP) and its relation with the anti-cancer effect of 5′-deoxy-5-fluorouridine (5'DFUR) and 5-fluorouracil (5FU) in T24 bladder cancer cells. Methods PD-ECGF/TP mRNA and protein expressions were determined by RT-PCR and Western blot,respectively.Cytotoxicity of 5′DFUR,5FU or mitomycin C (MMC) against T24 cells was evaluated by MTS assay. Results The expressions of PD-ECGF/TP mRNA and protein were concomitantly elevated in T24 cells after INF? treatment.And INF? decreased IC 50 s of 5′DFUR [from (16.0? 3.6 )mmol/L to (6.2?0.9)mmol/L,( P
4.A study of polymorphism of STR D13S325 locus in Chinese Han poplulation in Wuhan
Guohua CHEN ; Li CHEN ; Yuanfang ZHANG
Chinese Journal of Forensic Medicine 1986;0(02):-
Population genetic data of the STR D13S325 locus in a sample of 216 unrelated individuals of Han population in Wuhan was studied with the method of PCR. 11 alleles and 30 different genotypes were observed.There is no significant deviation from the Hardy Weinberg equilibrium.The DP value was 0 9292,PE 0 6137 and PIC 0 7755.The results of study of 200 meiosis showed no mutation.The results prove that this STR Locus is useful in the forensic sicence practice in Han population in Wuhan.
5.Exploration of SHDC in public hospital investor management
Jinghui LI ; Lirong ZHUGE ; Yuanfang YIN
Chinese Journal of Hospital Administration 2015;(8):585-587
The paper,based on a review of the reform of state-owned enterprises,and of the duty positioning and supervision of state-owned assets management committee, described experiences of Shanghai Hospital Development Center (SHDC ) in learning from such reforms. Centering onappropriate separation of ownership and power of management and defining public hospitals as key player of state-owned assets,SDHD explored to build a state-owned assets investor system conforming to the institutional positioning,assets category and operating targets of such hospitals.This way a state-owned assets management mode is gradually set up,featuring to make regulations beforehand,to supervise in the course and to supervise afterwards.
6.The Sensitizing Effect of Cetyltrimethylammonium Bromide onthe Resonance Light-Scattering Enhancement Between the Interactionof Deoxyribonucleic Acids and Brilliant Green
Xinhua HUANG ; Weiqun SHU ; Yuanfang LI ; Chengzh HUANG
Chinese Journal of Analytical Chemistry 2001;29(3):271-275
This is the first report of the sensitizing effect of cation surfactant on the enhanced resonance light scattering (RLS) resulted from the interaction of organic dyes with Deoxyribonucleic acid (DNA). On the conditions of pH 10.4 ~11.8 and ionic strength being less than 0.050, the interaction of brilliant green (BG) with DNA results in enhanced RLS signals at 398.0 and 467.2 nm overloaded some shoulder peaks in the enhanced region. It was found that the interaction was sensitized by the presence of cetyltrimethylammonium bromide (CTMAB). If 2.0~3.2×10-5 mol/L CMAB was used in the interacting medium, DNA in the range of 0~1.2 (mg/L could be detected with the limit of detection being less than 5 μg/L. Four synthetic samples were analyed with reproducibility
7.Effect of blood purification on extravascular lung water and permeability index in extrapulmonary acute respiratory distress syndrome
Wei ZHANG ; Weiwei CHEN ; Yuanfang WANG ; Jianping LI ; Yang YANG
Chinese Journal of Postgraduates of Medicine 2014;37(22):32-34
Objective To observe the effects of continuous blood purification(CBP) on extravascular lung water and permeability index in patients with severe extrapulmonary acute respiratory distress syndrome (ARDSexp).Methods Fifteen patients with severe ARDSexp were treated with CBP.The level of extravascular lung water index (EVLWI) and pulmonary vascular permeability index (PVPI) was measured before and after treatment,and at the same time the changes of inflammatory indicators were recorded.Results Compared with that before treatment,EVLWI and PVPI was decreased obviously after treatment (P < 0.01),the level of C-reactive protein and interleukin 6 (IL-6),calcitonin and systemic inflammatory response syndrome score and the acute physiology and chronic health Ⅱ score was significantly decreased (P < 0.01).Conclusion CBP can improve the level of EVLWI,PVPI and inflammatory indicators and also the prognosis of severe ARDSexp.
8.Study on effects of anti-DR5 monoclonal antibody on apoptosis and CDC effect in EC109 cells
Hongying HUANG ; Yaowu DU ; Huiling BAI ; Shulian LI ; Yuanfang MA
Chinese Journal of Immunology 2001;0(10):-
Objective:To observe anti-DR5 monoclonal antibody on apoptosis and CDC effect in EC109 cells.Methods:The anti-DR5 monoclonal antibody was prepared by hybridoma technique.Tumoricidal effects and complement-dependent cytotoxicity of the McAb to EC109 cells was screened by MTT assay.The apoptosis of EC109 cells was detected by flow cytometry with annexin Ⅴ-FITC/PI staining.Morphological change of EC109 was observed by microphotograph.Results:An anti-DR5 monoclonal antibody was obtained.It induced apoptosis of EC109 dose dependently.The cytotoxic action was notably enhanced by addition of complement.The cells growth inhibition ratios reached 83.04%.The apoptotic body and cathepsis were seen in microphotograph.Conclusion:The anti-DR5 monoclonal antibody could induce EC109 cells apoptosis and cause the complement dependent cytotoxic (CDC) effects powerfully.
9.Synergistic effect of anti-human DR5 monoclonal antibody and adriamycin on SMMC-7721 cells
Jun ZHANG ; Yuanfang MA ; Guangchao LIU ; Shulian LI ; Feng LU
Chinese Journal of Immunology 1985;0(02):-
Objective:To determine the sensitivity of SMMC-7721 cells to mDRA-6,the synergistic damage effect of mDRA-6 and adriamycin on human hepatocellular carcimoma cell lines and its possible mechanism.Methods:SMMC-7721 cells were cultured with RPMI1640 medium in regular condition.The morphology was observed by microscope.Cytotoxicity was examined by MTT assay. Apoptosis were detected by flow cytometry.Results:(1)The apoptosis of SMMC-7721 cells could be induced by mDRA-6,1.89 ?g/ml of mDRA-6 cound kill 36% of the cells;(2)Concentration-dependent cytotoxicity of adriamycin was exhibited in SMMC-7721 cells;(3)The combination of mDRA-6 and adriamycin exhibited synergistic effect on SMMC-7721 cells.2 ?g/ml of mDRA6 and 40 ng/ml of adriamycin killed 60%SMMC-7721.Conclusion:MDRA-6 can induce SMMC-7721 cell apoptosis.The combination of mDRA-6 and adriamycin exhibit synergistic effect on SMMC-7721 cells.
10.INFLUENCE OF TRACE ELEMENTS As, Se, Te, Re, Ir ON CELL PROLIFERATION OF TETRAHYMENA PYRIFORMIS
Renhuan TANG ; Xiaoming LI ; Qingxi ZHANG ; Yuanfang LIU ;
Acta Nutrimenta Sinica 1956;0(03):-
As an excellent unicellular biological model, Tetrahymena pyriformis S1 has been employed in studying the biological effects of the chemical elements. The nutritious and/or toxic effects on T. pyriformis S1 induced by As, Se, Te, Re, Ir have been observed. Trace amounts of As, Se, Re and Ir added in the peptone-glucose culture medium could stimulate the cell proliferation of T. pyriformis in the following range of concentration respectively: As 0.03-0.05 ppm, Se 0.01-1.0 ppm, Re 0.02-0.08 ppm, Ir 1.0-3.0 ppm, whereas Te only showed its inhibitory effect on the growth of cells.