Objective To study the effect of ultrasound guided curettage after methotrexate and mifepristone in the treatment of endogenous caesarean scar pregnancy.Methods A retrospective analysis was performed on 43 patients of endogenous caesarean scar pregnancy undergoing treatment of ultrasound guided curettage after methotrexate and mifepristone.Results 39 cases were successful,4 cases were transformed to laparotomy because of intraoperative blood loss,7 cases of bleeding after curettage were successful by uterine carity placed double lumina Foley catheter. Conclusion Ultrasound guided curettage after methotrexate and mifepristone is a safe,effective,little trauma and low cost method in the treatment of endogenous caesarean scar pregnancy,it especially adapts to primary hospital.

Mental Health Law has specify the mental disorder patients’ informed consent right,but in practice, the problems,the mental health status of patients, namely the families’ right of subrogation exercise,“loss of self-control or deny having mental disorder”, have prevented the exercising of informed consent right. Therefore,it is necessary to effectively solve this plight of rebuilding a harmonious relationship between doctors and patients,estab-lishing mentally disordered patients’ right to advance directives, especially choosing the instrument of assessing the individual’ s capacity to consent.

Population genetic data of the STR D13S325 locus in a sample of 216 unrelated individuals of Han population in Wuhan was studied with the method of PCR. 11 alleles and 30 different genotypes were observed.There is no significant deviation from the Hardy Weinberg equilibrium.The DP value was 0 9292,PE 0 6137 and PIC 0 7755.The results of study of 200 meiosis showed no mutation.The results prove that this STR Locus is useful in the forensic sicence practice in Han population in Wuhan.

The paper,based on a review of the reform of state-owned enterprises,and of the duty positioning and supervision of state-owned assets management committee, described experiences of Shanghai Hospital Development Center (SHDC ) in learning from such reforms. Centering onappropriate separation of ownership and power of management and defining public hospitals as key player of state-owned assets,SDHD explored to build a state-owned assets investor system conforming to the institutional positioning,assets category and operating targets of such hospitals.This way a state-owned assets management mode is gradually set up,featuring to make regulations beforehand,to supervise in the course and to supervise afterwards.

Objective To investigate the regulative effect of interferon gamma (INF?) on the expression of platelet-derived endothelial cell growth factor / thymidine phosphorylase (PD-ECGF/TP) and its relation with the anti-cancer effect of 5′-deoxy-5-fluorouridine (5'DFUR) and 5-fluorouracil (5FU) in T24 bladder cancer cells. Methods PD-ECGF/TP mRNA and protein expressions were determined by RT-PCR and Western blot,respectively.Cytotoxicity of 5′DFUR,5FU or mitomycin C (MMC) against T24 cells was evaluated by MTS assay. Results The expressions of PD-ECGF/TP mRNA and protein were concomitantly elevated in T24 cells after INF? treatment.And INF? decreased IC 50 s of 5′DFUR [from (16.0? 3.6 )mmol/L to (6.2?0.9)mmol/L,( P

This is the first report of the sensitizing effect of cation surfactant on the enhanced resonance light scattering (RLS) resulted from the interaction of organic dyes with Deoxyribonucleic acid (DNA). On the conditions of pH 10.4 ～11.8 and ionic strength being less than 0.050, the interaction of brilliant green (BG) with DNA results in enhanced RLS signals at 398.0 and 467.2 nm overloaded some shoulder peaks in the enhanced region. It was found that the interaction was sensitized by the presence of cetyltrimethylammonium bromide (CTMAB). If 2.0～3.2×10-5 mol/L CMAB was used in the interacting medium, DNA in the range of 0～1.2 (mg/L could be detected with the limit of detection being less than 5 μg/L. Four synthetic samples were analyed with reproducibility

As an excellent unicellular biological model, Tetrahymena pyriformis S1 has been employed in studying the biological effects of the chemical elements. The nutritious and/or toxic effects on T. pyriformis S1 induced by As, Se, Te, Re, Ir have been observed. Trace amounts of As, Se, Re and Ir added in the peptone-glucose culture medium could stimulate the cell proliferation of T. pyriformis in the following range of concentration respectively: As 0.03-0.05 ppm, Se 0.01-1.0 ppm, Re 0.02-0.08 ppm, Ir 1.0-3.0 ppm, whereas Te only showed its inhibitory effect on the growth of cells.

Objective:To observe anti-DR5 monoclonal antibody on apoptosis and CDC effect in EC109 cells.Methods:The anti-DR5 monoclonal antibody was prepared by hybridoma technique.Tumoricidal effects and complement-dependent cytotoxicity of the McAb to EC109 cells was screened by MTT assay.The apoptosis of EC109 cells was detected by flow cytometry with annexin Ⅴ-FITC/PI staining.Morphological change of EC109 was observed by microphotograph.Results:An anti-DR5 monoclonal antibody was obtained.It induced apoptosis of EC109 dose dependently.The cytotoxic action was notably enhanced by addition of complement.The cells growth inhibition ratios reached 83.04%.The apoptotic body and cathepsis were seen in microphotograph.Conclusion:The anti-DR5 monoclonal antibody could induce EC109 cells apoptosis and cause the complement dependent cytotoxic (CDC) effects powerfully.

Objective To explore the effects of magnesium valprote on behaviors and lipid peroxidation of brain in chronic stress depression mice.Methods 40 male mice were randomly divided into normal control group,model group, amitriptyline control group and magnesium valprote group, and.10 mice each group.Except normal control group,other groups were separated one mouse in each box and exposed to 21 days chronic unpredictable stressors, and the mice of normal control group were fed in a box and stress-free.During the stress, the mice of amitriptyline control group were applied amitriptyline(0.2 mg/10g) by intragastric every day,magnesium valprote group were given intragastrically magnesium valprote (0.8 mg/10g), and the mice of model and normal control group were administrated with normal sodium of the same volume by the same method.On 7th, 14th,21st day, all mice weight were weighed and weight gain was calculated.The ethology examination was performed by using jigglecage connected with Biology Function Experiment System and experiment of sucrose solution consumption after the chronic stress.At the end of experiment, all mice were killed, and then their brains were removed and made into brain homogenate.The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of malondialdehyde(MDA) in brain homogenate were analyzed by spectrophotometry.Results Compared with model group, magnesium valprote group were increased on locomotor activity number(97.50 ± 20.54,302.30± 39.04, t = 31.1432, P ＜ 0.01 ), sucrose solution consumption ( ( 15.30 ± 3.60 ) ml, ( 23.70 ± 4.45 ) ml, t =9.8446, P＜0.01) ,body weight gain(( 10.56 ± 1.53)g,( 12.63 ±2.12)g, t=5.3113, P=0.007) ,the activity of SOD( t =3.8428, P=0.004) ,and GSH-Px( t= 3.4143, P=0.007), but decreased on irritation number( t =17.1370, P ＜ 0.01 ) and the content of MDA( t= 11.0778, P = 0.002).Conclusion Magnesium valprote can ameliorate behavior impairment of chronic stress-induced depression mice, and the observed antidepressant-like effects may be related to inhibition lipid peroxidation of mice brain.

Objective To observe the effects of continuous blood purification(CBP) on extravascular lung water and permeability index in patients with severe extrapulmonary acute respiratory distress syndrome (ARDSexp).Methods Fifteen patients with severe ARDSexp were treated with CBP.The level of extravascular lung water index (EVLWI) and pulmonary vascular permeability index (PVPI) was measured before and after treatment,and at the same time the changes of inflammatory indicators were recorded.Results Compared with that before treatment,EVLWI and PVPI was decreased obviously after treatment (P ＜ 0.01),the level of C-reactive protein and interleukin 6 (IL-6),calcitonin and systemic inflammatory response syndrome score and the acute physiology and chronic health Ⅱ score was significantly decreased (P ＜ 0.01).Conclusion CBP can improve the level of EVLWI,PVPI and inflammatory indicators and also the prognosis of severe ARDSexp.