Objective:To provide the methodology reference for the gene expression data clustering.Methods:This paper proposed a fuzzy clustering ensemble algorithm based on wavelet de-noising.The new method is applied to the yeast cell's gene expression data,and the clustering results were compared with fuzzy ensemble clustering methods.Results:Apply micro-precision to evaluate the clustering results.The research indicated that the proposed method was superior to fuzzy ensemble clustering algotuthm.Conclusion:The proposed method has better clustering for the gene expression data clustering.

BACKGROUND: PI3K is a family of enzymes involved in insulin signal transductlon pathway, the abnormal expression of which would affect synthesis, secretion, and migration of GLUT4, therefore, results In increased blood sugar, eventually, leads to diabetes.OBJECTIVE: To discuss the effects of insulin combined with selenium on PI3K and GLUT4 expression in cellular signal transduction of skeletal musde in diabetic rats.METHODS: Sprague Dawley (SD) rats were randomly divided into the control, diabetic, insulin-treated diabetic, selenium-treated diabetic and combination administration groups. All rats were prepared for diabetic models by injecting 50 mg/kg Streptozocin exception of the control group. Rats in the control and diabetic groups ware free to food and water; in the insulin treatment group,rats were subcutaneous injected 1 U/(kg·d) insulin. Rats in the selenium treatment group ware treated with a dose of 180 pg/kg per day of sodium selenite dissolved in redistilled water by gavage; and in the combination administration were given both treatments for 4 successive weeks. The levels PI3K end GLUT4 in skeletal muscle were estimated using Western blotting and immunohistochemistry techniques.RESULTS AND CONCLUSION: Immunohietochemistral results were accordance to Western blotting results, which showed the combination of insulin and selenium can remarkable Increase the levels of PI3K in skeletal muscle and GLUT4 in skeletal muscle membrane fraction, therefore, enhance the insulin signal transduction pathway.

Objective To investigate the relation between omentin and coronary artery disease.Methods Eighty-eight eases were divided into 2 groups, coronary artery disease group (56 cases, includingacute coronary syndrome 28 cases and stable angina 28 cases), and non-coronary artery disease group (32cases). The plasma omentin levels were assayed by ELISA, and height, weight, blood pressure, totalcholesterol high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C),lriglyeeride (TG), high-sensitivity C-reactive protein were detected. Results The plasma omentin level incoronary artery disease group [(33.4 + 6.4) μg/L] was lower than that in non-coronary artery disease group [(51.8 + 7.7) μg/L](P= 0.034). Logistic analysis showed that the decline of omentin was an independent risk factor of coronary artery disease (P=0.012). Conclusions The decline of omentin is an independent risk factor of coronary artery disease. The plasma omentin may contribute, in part, to the pathogenesis of coronary artery disease.

Objective:To inspect and improve physician professionalism effectively and preliminarily build eval-uation index system for physician professionalism. Methods:A sample of 240 physicians selected randomly from 8 Three-A hospitals in Shanghai were investigated using a self-designed questionnaire. Then the method of analyt-ic hierarchy process was performed in this study to construct the evaluation model of physician professionalism and determine every index weight. Results: An index system was initially built for physician professionalism evalua-tion. This system comprised three level-1 indexes namely moral quality characteristics, achievement action char-acteristics and vocational cognitive characteristics, as well as 14 level-2 indexes that drew from level-1 including responsibility etc. , at the same time determined their weight distribution. The judgment matrix had completely con-sistency through consistency check, indicating that the weight coefficient can reflect the relative importance of every index. Conclusion:Moral quality characteristics is one of the most important components of physician professional-ism evaluation; responsibility consciousness and vocational ideal are vital for the growth of physicians; lifelong learning and communication skills with high weight reflects needs of modern health care industry development;medical ethics consciousness has a trend of strengthening in physician groups, and enhancing evaluation index sys-tem for physician professionalism has the operability.

Objective To analyze perioperative nursing methods of chest wall tumor removal and chest wall reconstruction. Methods 12 patients with chest wall tumor removal and chest wall revascularization were choosed as the research objects,and which were given prospective nursing intervention combined with high quality nursing intervention. Results 12 patients all cured without one case death occurred, and 4 cases complications. Followed up for 6 to 36 months, no local recurrence of alienation, chest wall deformity and rejection. Conclusions Proactive nursing intervention help to predict perioperative complications. High-quality nursing care including psychological nursing, skin nursing, pain nursing, function training etc, can promote patients recovery.

Bone remodeling is an ordered process of coupling of bone resorption and bone formation. Cytokines secreted by different bone cells and membrane molecules expressed by bone cells are involved in regulation of coupling of bone resorption and bone formation. To reveal the mechanism of bone coupling is of great significance in the study and diagnosis of osteoporosis and other metabolic bone diseases. In the present review, we summarize the progression of mechanisms by which cytokines and the related membrane molecules are regulating the coupling of bone resorption and bone formation.

Objective To investigate the risk factors of Gallstone Disease ( GD) by a meta-analy-sis, which hopefully will help to develop a strategy for its prevention .Methods Thirty case-control studies on risk factors of GD in Chinese patients reported from 1987 to 2014 were included .A quantitative compre-hensive analysis and a heterogeneity test were conducted and the OR value and its 95% confidence interval (CI) were calculated by using Stata 12.0.Results A family history of GD [OR(95%CI):2.73(2.22~3.35)], alcohol drinking [OR(95%CI):1.53(1.20~1.97)], a history of biliary tract roundworm [OR (95%CI):5.28(3.10 ~9.00)], a history of biliary tract infection [OR(95%CI): 28.50(19.22 ~42.26 ) ] , a BMI≥25 kg/m2 [ OR ( 95%CI ):1.70 ( 1.51~1.92 ) ] , greasy food intake [ OR ( 95%CI ):3.27(1.76~6.07)], a non-vegetarian diet [OR(95%CI):2.03(1.48~2.77)], hyperlipidemia [OR (95%CI):2.26(1.88~2.71)], menopause [OR(95%CI):3.25(2.49~4.24)] and multiple preg-nancies [OR(95%CI):1.56(1.29~1.88)] were risk factors of GD in China.Besides, milk drinking [OR(95%CI):0.43(0.34~0.55)] and regular breakfast [OR(95%CI):0.19(0.06~0.64)] were protective factors of GD.Tea drinking [OR(95%CI): 0.43(0.34 ~0.55)], smoking [OR(95%CI):0.86(0.51~1.45)], and a vegetarian diet [OR(95%CI):0.24(0.05~1.13)] were possible GD’s protective factors as they were not statistically significant .Conclusion Having breakfast , drinking milk , keeping fit , less alcohol and avoiding Ascaris infection prevent the occurrence of GD in China .

Objective:To investigate and compare the influences of Ghrelin and growth hormone releasing peptide 6(GHRP-6) on the contractility of stomach smooth muscle in guinea pigs,and to study the related mechanism.Methods: The myenteric plexuses of gastric fundus and antrium in guinea pigs were stimulated with electrical field stimulation(EFS) to observe the influence of Ghrelin and GHRP-6 on the contractility of stomach smooth muscle.The influences of N-nitro-L-arginine(L-NNA) and L-Arginine(L-AA) on the effect of Ghrelin and GHRP-6 were studied to disclose the mechanism of the effects of Ghrelin and GHRP-6.Results: The circular muscle tissues of the gastric fundus generated on-relaxations and off-contractions when stimulating myenteric plexuses with 1-16 Hz electrical field;the on-responses induced relaxation could be reduced by L-NNA and the off-contractions induced contraction could be blocked by atropine and guanethidine.In fundic strips,ghrelin and GHRP-6 could decrease the on-response induced relaxation and increase off-response induced contraction of the muscle,with the effect of Ghrelin obviously stronger than that of GHRP-6.L-NNA could increase the effects of Ghrelin and GHRP-6-induced muscle contraction,and L-AA could decrease their effects.In the antral strips,electrical field stimulation of myenteric plexuses led to disappearance of relaxation wave,only leaving off-contractions.Both ghrelin and GHRP-6 could increase that contraction.Conclusion: Both ghrelin and GHRP-6 can promote the contractility of stomach smooth muscle in guinea pigs through stimulating myenteric plexuses of gastric fundus and antrium,which might be related to the NO pathway.

Objective To explore the role of CD95 system in chemotherapeutic sensitivity of pancreatic cancer cells, in an attempt to enhance the efficacy of chemotherapy by transfection CD95 gene, and to provide the evidence for the immunological treatment of pancreatic cancer. Methods CD95 gene was transfected into the pancreatic cancer cell line SW1990 by lipofectamine. The transfected cells were selected by G418. CD95 expressions of the transfected cells were detected by Northern blot and Western blot. MTT assay was used to analyze the response of the transfected cells to 5 fluorouracil, adriamycin (ADM), gemcitabine and in combination with anti CD95 monoclonal antibody (mAb). The drug induced apoptosis of transfected cells was measured by flow cytometry. Results The transfected pancreatic cancer cell SW1990 could overexpress CD95 stably. The CD95 mRNA and protein expressions were significantly increased in the transfected cells than in the controls. Anti CD95 mAb could inhibit the growth of the transfected cells. In addition, transfected cells were more sensitive to clinically relevant concentrations of chemotherapeutic drugs than non transfected cells. Anti CD95 mAb addition could enhance the cytotoxic effect of chemotherapeutic drugs. Drug induced apoptosis in ADM treated transfected cells more pronounced than in non transfected cells. Conclusions CD95 transfection could increase the sensitivity of pancreatic cancer cell SW1990 to, and partly reverse the resistance to, chemotherapeutic drugs. The combination of chemotherapeutic drugs with anti CD95 mAb showed a synergistic cytotoxicity to pancreatic cancer cells.

Objective To evaluate the effect of remifentanil on heart rate variability,to discuss the optimal does of remifentanil for tracheal intubation.Methods Thirty six ASA Ⅰ～Ⅱ patients were randomly assigned to one of three groups(n=12/group)according to the remifentanil target plasma concentration(2、4、6ng/mL).No premedication was given.The target-controlled infusion(TCI)of renifentanil was initiated 5 minutes after the TCI of propofol given at the target plasma concentration of 3.5?g/mL.Mean arterial pressure(MAP),heart rate(HR),HF,LF,HF/LF were measured from baseline values to 5 minutes after the operation began every minutes.MAP,HR,HF,LF and HF/LF were obtained on the time point as follows:before the induction(T0),before the start of remifentanil TCI(T1),before laryngoscopy(T2),and maximum values after intubation(T3),before operation(T4),maximum values after operation.All the data obtained were analyzed used ANOVA with SPSS 11.5 statistical software.P