Objective: To investigate the regulatory role of LKB1 gene on expression of vascular endothelial growth factor (VEGF) in lung cancer cell line A549. Methods: DNA fragment encoding LKB1 protein was amplified by Nest-PCR from human fetal brain cDNA library and was sub-cloned into the eukaryotic expression vector pcDNA3. 1. The recombinant vector was transferred into A549 cells by Lipofectamine and screened by G418. RT-PCR and Western blot were used to study the expression of LKB1, SP1 and VEGF in A549 cells. Transcription factor SP1 was silenced by small interference RNA (siRNA); RT-PCR and Western blot were applied to examine the changes of SP1 and VEGF gene. Results: DNA sequencing analysis revealed that the open reading frame of LKB1 gene was successfully cloned into the expression vector. Stable cell line of A549 expressing exogenous LKB1 was constructed. LKB1 remarkably suppressed SP1 and VEGF expression. SiRNA targeting SP1 effectively decreased the expression of SP1 and SP1 silencing caused remarkable down-regulation of VEGF expression. Conclusion: LKB1 can negatively regulate the expression of VEGF by negatively regulate the expression of transcription factor SP1.

OBJECTIVE:To investigate the incidence and clinical manifestations of adverse drug reactions(ADR)induced by Chinese drugs preparations in hospitals of Ya'an district and discuss the rational dosage regimen of Chinese drugs preparations.METHODS:The adverse drug reactions(ADR)induced by Chinese drugs preparations in hospitals of Ya'an district occurred from Jan.to Dec.in 2008 were analyzed statistically.RESULTS:The ADR induced by Chinese drugs preparations occurred via intravenous drip represented 76.8% of the total;lesions the skin and its appendages the major system-organ involved,represented 31.3% of the total;18.3% were induced by Shuanghuanglian injections,and serious ADR accounted for 4.0%.CONCLUSION:It is important to use traditional medicine preparations rationally so as to reduce or avoid the incidence of ADR.

Innovative medical talents training in higher medical colleges and universities is the requirements of the era. Based on the medical education of Xi'an Jiaotong University,the article systematically introduces some thoughts and methods of talents training system,which includes three aspects such as three-dimensional teaching environment(teaching resource construction,platform construction and construction of teachers' team),experiment curriculum system reform and innovation mechanism,etc. Construction of three-dimensional teaching environment is emphasized.

Objective To observe effects of ginsenoside Rb1 on phosphorylation of microtubule-associated protein 2 (pMAP-2) in hippocampus and amygdala of depressive model rats. Methods Thirty male Wistar rats were randomly divided into control group, model group and treatment group. The depression rat model was produced by giving chronic unpredicted mild stress (CUMS). Treatment group was given daily intragastric administration of ginsenoside RB 1 (1 g/mL crude drug, 1 mL/100 g body weight) for 22 days during modeling. Western blot assay was used to detect expressions of MAP-2 and pMAP-2 protein, and real-time PCR was used to detect expressions of pMAP-2 mRNA respectively. Results The expressions of pMAP-2 protein and mRNA in hippocampus and amygdala were significantly lower in model group than those of control group (P < 0.05). The expressions of pMAP-2 protein and mRNA were significantly higher in treatment group than those of model group (P<0.05). Conclusion Ginsenoside Rb1 can play anti-depression role by inhibiting the phosphorylation of MAP-2 in rats.

Objective To investigate the effect of ceftidine in operating room intervention and its effect on the quality of intervention. Methods Select 2016 May to May 2017, hangzhou normal university affiliated hospital operating room of our hospital, 366 cases of patients with skull, 2 groups randomly divided into two groups, control group routine surgery, the team combined use of cephalosporins he totally, compared two groups of two groups patients therapeutic effect. Results The intracranial infection was significantly lower in the two groups than in the control group (P<0.05). In the two groups, the number of live bacteria and the comparison of enzyme value were significantly lower than that in the control group (P <0.05). Intervention quality compared two groups of 2 groups of patients, the team of PCT in patients with calcitonin (former) and CRP (CRP), TNF alpha (tumor necrosis factor alpha) and white blood cell count were significantly lower than control group (P< 0.05). Conclusion Children with oral ulcer treatment, the therapeutic effect of smecta ideal, clinical application shall be the head to prevent the use of cephalosporins in patients with surgical intervention in the operating room he totally, can improve the quality of intervention, reduce the incidence of infection.

Neurolymphomatosis(NL) is characterized by lymphomatous infiltration of the peripheral nervous system. We report a case of neurolymphomatosis(NL) which was confirmed by sural nerve biopsy. Sural nerve specimen from a 49-year-old female patient with weakness of limbs was examined with routine histochemical and immunohistochemistry staining, in which the first antibodies against CD3, CD20, CD45, CD45RO and CD68 were used. Numerous T-lymphoma cells invaded in the adipose tissue of epineurium of sural nerve. The nerve biopsy showed marked axonal degeneration of myelinated fibers. The clinical and histopathologic findings confirmed the diagnosis of neurolymphomatosis.

Objective To optimize the exposure parameters and reduce the irradiation dose level in infants and young children during digital radiography (DR) chest radiography under the premise of satisfied image quality.Methods The thoracic thickness of 100 patients were measured.Determined the aluminum equivalent of the thoraxes of the infants and young children by comparing the grayscale value and the aluminum step wedge.Another 100 infants and young children of experimental exposure were performed with the aluminum step wedge as a phantom,under AEC control,kV was the only variant to explore the optimal exposure parameters with dose monitor simultaneously.At last,clinical validation was performed.Images quality was compared with x2 test.The radiation dose of two groups was compared with t test.Results The maximum,minimum,average thoracic thickness and their correspondent aluminum equivalent were 13.5 and 2.3 cm,8.0 and 1.4 cm,(10.6 ± 1.3) and 2.0 cm,respectively.The average thoracic thickness of experimental group was (10.1 ± 2.2) cm.The range of entrance surface dose was 0.068-0.056 mGy while the tube voltage range was 55-65 kV.The exposure index range was 0.60-0.74.The visual inspection of aluminum step wedge was from grades 8 to 11 with satisfying image quality at lower radiation.The infant chest X-ray photography exposure parameters formula have been optimized,that was kV =thoracic thickness (cm) × 2 + 38 (constant),mAs (0.8-1.0) with SID =100 cm,without filter grid.Compare to the conventional parameters,the image quality of new method had no significant differences (P ＞ 0.05).The actual average entrance surface dose was (0.048 ± 0.007) mGy,lower than AEC group (0.066 ± 0.008) mGy.The difference was statistically significant (t =16.781,P ＜ 0.001).Conclusions The optimized formula kV =thoracic thickness (cm) ×2 + 38(constant),mAs (0.8-1.0) with SID =100 cm was credible for lowering the radiation exposure with good image quality for clinical diagnosis.

Unified examination refers to the examination given by the university for medical students to graduate after they pass six curriculum examinations,including diagnostics,introduce of surgery,internal medicine,surgery,obstetrics and gynecology and pedology during the clinical study phases.It is a necessary process for cultivation of the professionals of the clinic medicine and has practice significance.Through the organization and management of the unified examination process,as well as analysis of examination result and feedback,education quality has been enhanced and educational reform promoted.

Aiming at building research disciplines,Xijing hospital has initially achieved a strategic transformation into a hospital with research disciplines,with such measures as scientific layout of disciplines,making of advantageous disciplines with overseas benchmarks,encouragement of potential disciplines with advantageous disciplines,promotion of medical innovation with innovative ideas,and upgrading clinical service quality with technical innovation.

A series of isoindoline derivatives were designed, synthesized, and evaluated for their double inhibitory activities. All of them were new compounds, and their structures were confirmed by 1H NMR and HR-MS. Preliminary in vitro pharmacological tests showed that all compounds exhibited 5-HT or NE reuptake inhibition activity. Among the tested compounds, compound I-3 exhibited potent inhibitory activity against 5-HT and NE reuptake in vitro, and exhibited potent antidepressant activity in vivo. These compounds designed can be further optimized for finding more potent 5-HT/NE dual reuptake inhibitors and antidepressant candidates as well.