Objective To study the expression and significance of P57KIP2 in complete mole(CM ) and partial mole(PM).Methods The expression of P57KIP2 in histology unequivocal 45 cases of CM and 30 cases of PM was detected by immunohistochemistry method.Results In the PMs,expression of P57kip2 was strongly and continuously(30/34),in the CMs,it was absent or weak expressed (5/45).The difference of P57K1P2 expression between PMs and CMs was statistically significant( P ＜ 0.05 ).Conclusion P57KIP2 immunostaining is a sensitive sign of partial moleand in differential diagnosis of PM from CM and it is deserving of clinical application.

OBJECTIVETo observe the clinical efficacy of BO's abdominal acupuncture for obese type-2 diabetes mellitus (T2DM).

METHODSSixty patients of obese T2DM were randomly divided into an acupuncture group and a medication group, 30 cases in each one. Patients in the medication group were treated with basic treatment combined with oral administration of regular antidiabetics, three weeks as one session. Patients in the acupuncture group, based on the medication group, were treated with abdominal acupuncture at Yinqiguiyuan [Zhongwan (CV 12), Xiawan (CV 10), Qihai (CV 6), Guanguan (CV 4)], Fusiguan [Huaroumen (ST 24), Wailing (TE 5)], Tianshu (ST 25), Daheng (SP 15), Qixue (KI 13), etc.; the treatment was given three times per week, 3 weeks as one session. The systolic blood pressure (SBP), diastolic blood pressure (DBP), body weight, waist circumference (WC), hip circumference, body mass index (BI) were observed before and after treatment in the two groups, and fasting plasma glucose (FPG), fasting insulin (FINS), 2-hours postprandial blood glucose by oral glucose tolerance test (OGTT) and insulin, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), HOMA-IR of insulin resistance index were calculated and adverge events were recorded.

RESULTSCompared before the treatment, SBP, WC, body weight, BMI, FPG, OG-TT2hBG, FINS, GTT2h insulin, HOMA-IR, TC and LDL-C in the acupuncture group were all significantly reduced (all P <0. 05), while FPG, OGTT2H insulin and TG were increased in the medication group (all P<0. 05)'. The differences of reducing SBP, WC, FPG, OGTT2H insulin, HOMA-IR, TC, TG and LDL-C were statistically significant between the two groups (all P<0. 05). The total effective rate was 93. 3% (28/30) in the acupuncture group, which was significantly superior to 23. 3% (7/30) in the medication group (P<0. 01).

CONCLUSIONBO's abdominal acupuncture has obvious clinical efficacy for obese type-2 diabetes mellitus, featuring in lowering blood pressure, reducing weight, decreasing blood glucose, im- proving insulin resistance and lowering lipid, which has no adverse effects and is worthy of clinical popularization and application.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Blood Glucose ; metabolism ; Body Mass Index ; Body Weight ; Cholesterol ; blood ; Diabetes Mellitus, Type 2 ; metabolism ; physiopathology ; therapy ; Female ; Humans ; Insulin ; blood ; metabolism ; Lipoproteins, LDL ; blood ; metabolism ; Male ; Middle Aged ; Triglycerides ; blood ; Waist Circumference

Anaphylaxis ; chemically induced ; Anti-Bacterial Agents ; adverse effects ; Azithromycin ; adverse effects ; Fatal Outcome ; Humans

Diabetes Mellitus, Type 2 ; drug therapy ; Humans ; Hypoglycemic Agents ; therapeutic use ; Non-alcoholic Fatty Liver Disease ; drug therapy

Objective To study the DNA damage induced by dichloroacetic acid(DCA) and trichloroacetic acid(TCA) which are from drinking water disinfection by-products. Methods V79 cells and hepatocytes of mice were treated with DCA and TCA for 1 hour and then were tested by comet assay. After stained by EB, tail length of the cells were observed and counted under the fluorescence microscope. Results Both DCA and TCA could result in the increasing of average tail length of the treated cells whether they were V79 cells or hepatocytes of mice and the dose-response relationships were seen. Conclusion Both DCA and TCA can cause DNA damage of mammalian cells and this may be related to the carcinogenic mechanism. DCA and TCA belong to genetic carcinogens.

Quercetin is the major bioflavonoid in the human diet and can be ubiquitously found in nature.Studies have suggested that quercetin has many biological activities and clinical applications such as antioxidant,anti-inflammation, anti-tumor effects. This article reviews recent advances in its anti-oxidation mechanisms, protective effects on oxidative stress-related diseases, and other applications.

Quantum dots (QD),a kind of nanocrystal,are made from Ⅱ-Ⅵ or Ⅲ-Ⅴ group elements.It has been reported that compared with current conventional fluorescent markers,QD have excellent optical properties such as strong fluorescence,photochemical stability and can be used for simultaneous multi-channel imaging.Meanwhile,as nanoparticle,QD can be easily surface modified with a variety of biological molecular and can reach cancer cells easily by penetrating the tumor angiogenesis.Therefore,QD have unique advantages in targeted real-time visual imaging of in vivo tumor and have great prospects in the individual diagnosis and treatment of tumor.However,the long-term biosafety after QD into the body still needs further study.

Dexamethasone ; therapeutic use ; Glucocorticoids ; pharmacology ; therapeutic use ; Humans ; Infant, Newborn ; Meconium Aspiration Syndrome ; drug therapy ; physiopathology ; Methylprednisolone ; therapeutic use ; Prednisolone ; therapeutic use

Objective To explore the multi-resistance pattern of 88 strains of isoniazid-resistant Mycobacteria in recent years,so as to provide reference for prevention and treatment of tuberculosis.Methods Absolute concentration method was applied to performing drug susceptibility test for 181 isolates of Mycobacteria to 10 kinds of antitubercuIosis drugs. Results The primary resistance rate was 19.3%,and the acquired drug resistance rate Was 55.8%.The percentage of isoniazid-or double resistant-Mycobacteria was 37.6%.The Mycobacterium which was resistant to three or above drugs accounted for 85.2 %.Conclusion The majority of isoniazid-resistant Mycobacterium is a multi-resistant strain,which should be thought highly in prevention and treatment of tuberculosis to control or reduce the initial and acquired resistance rates.

Objective:To ensure the authenticity, scientific and reliability of medical devices clinical trials results.Methods: The drug clinical research institute office set up full-time secondary quality control team, clarified the responsibility, made application form, problem check and track list, subjects’ identity registration form. Using computer network to make 19 medical equipment quality control of clinical trials.Results: Assure the rights and safety of subjects, the validity, accuracy and completeness of clinical trial data and clinical trial results.Conclusion: The drug clinical research institute office, full-time secondary quality control team made strict clinical trial project for the whole process of quality control and ensure the true, scientific and reliable results.