Objective To study the expression and significance of P57KIP2 in complete mole(CM ) and partial mole(PM).Methods The expression of P57KIP2 in histology unequivocal 45 cases of CM and 30 cases of PM was detected by immunohistochemistry method.Results In the PMs,expression of P57kip2 was strongly and continuously(30/34),in the CMs,it was absent or weak expressed (5/45).The difference of P57K1P2 expression between PMs and CMs was statistically significant( P ＜ 0.05 ).Conclusion P57KIP2 immunostaining is a sensitive sign of partial moleand in differential diagnosis of PM from CM and it is deserving of clinical application.

Diabetes Mellitus, Type 2 ; drug therapy ; Humans ; Hypoglycemic Agents ; therapeutic use ; Non-alcoholic Fatty Liver Disease ; drug therapy

Anaphylaxis ; chemically induced ; Anti-Bacterial Agents ; adverse effects ; Azithromycin ; adverse effects ; Fatal Outcome ; Humans

OBJECTIVETo observe the clinical efficacy of BO's abdominal acupuncture for obese type-2 diabetes mellitus (T2DM).

METHODSSixty patients of obese T2DM were randomly divided into an acupuncture group and a medication group, 30 cases in each one. Patients in the medication group were treated with basic treatment combined with oral administration of regular antidiabetics, three weeks as one session. Patients in the acupuncture group, based on the medication group, were treated with abdominal acupuncture at Yinqiguiyuan [Zhongwan (CV 12), Xiawan (CV 10), Qihai (CV 6), Guanguan (CV 4)], Fusiguan [Huaroumen (ST 24), Wailing (TE 5)], Tianshu (ST 25), Daheng (SP 15), Qixue (KI 13), etc.; the treatment was given three times per week, 3 weeks as one session. The systolic blood pressure (SBP), diastolic blood pressure (DBP), body weight, waist circumference (WC), hip circumference, body mass index (BI) were observed before and after treatment in the two groups, and fasting plasma glucose (FPG), fasting insulin (FINS), 2-hours postprandial blood glucose by oral glucose tolerance test (OGTT) and insulin, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), HOMA-IR of insulin resistance index were calculated and adverge events were recorded.

RESULTSCompared before the treatment, SBP, WC, body weight, BMI, FPG, OG-TT2hBG, FINS, GTT2h insulin, HOMA-IR, TC and LDL-C in the acupuncture group were all significantly reduced (all P <0. 05), while FPG, OGTT2H insulin and TG were increased in the medication group (all P<0. 05)'. The differences of reducing SBP, WC, FPG, OGTT2H insulin, HOMA-IR, TC, TG and LDL-C were statistically significant between the two groups (all P<0. 05). The total effective rate was 93. 3% (28/30) in the acupuncture group, which was significantly superior to 23. 3% (7/30) in the medication group (P<0. 01).

CONCLUSIONBO's abdominal acupuncture has obvious clinical efficacy for obese type-2 diabetes mellitus, featuring in lowering blood pressure, reducing weight, decreasing blood glucose, im- proving insulin resistance and lowering lipid, which has no adverse effects and is worthy of clinical popularization and application.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Blood Glucose ; metabolism ; Body Mass Index ; Body Weight ; Cholesterol ; blood ; Diabetes Mellitus, Type 2 ; metabolism ; physiopathology ; therapy ; Female ; Humans ; Insulin ; blood ; metabolism ; Lipoproteins, LDL ; blood ; metabolism ; Male ; Middle Aged ; Triglycerides ; blood ; Waist Circumference

Objective To investigate the significance of clinical nursing pathway in perioperative period of children with hypospadia.Methods 63 cases of hypospadia children patients were randomly divided into the control group (30 cases)and the experimental group (33 cases).The traditional nursing method was used in the control group,while the clinical nursing pathway was used in the experimental group.Results The hospital stay,hospital charges and complications of the experimental group were less than the control group.The satisfaction degree of the experimental group was higher than the control group.There was significant difference.Conclusions Clinical nursing pathway is a safe and effective nursing model.It can shorten hospital stay,reduce hospital charges,decrease complications,and improve patients' satisfaction degree.

Objective:To establish a method for the determination of residual 5 heavy metal elements in single use and gravity feed infusion sets and intravenous infusion needles, including Cd, Cr, Cu, Sn and Pb. Methods:After the pre-preparation,the sample was dissolved in 1% nitrate. The 5 heavy metal elements in the sample solution were determined by inductively coupled plasma mass spec-trometry (ICP-MS). Results:Under the ICP-MS assay conditions, each element showed good linearity within the range of 0-50 ng· ml-1(r>0. 999 0). The average recoveries were within the range of 92%-116% and 1. 6%-2. 9%, respectively (RSD<3,n=9). Conclusion:The operation is simple and can meet the demand for the analysis of heavy metal elements in single use and gravity feed infusion sets and intravenous infusion needles.

Education of medical morality is an important part of medical education and must be through the whole career life of medical workers.Now our society is in the period of transition,but education of medial morality fails to get enough attention.Based on the present situation,the paper first makes a systematic exposition of the importance,intension,element and process of the reception of medical morality education of medical workers,then points out the rule of this reception and the approach to realize the validity of medical morality education.

Objective To study the DNA damage induced by dichloroacetic acid(DCA) and trichloroacetic acid(TCA) which are from drinking water disinfection by-products. Methods V79 cells and hepatocytes of mice were treated with DCA and TCA for 1 hour and then were tested by comet assay. After stained by EB, tail length of the cells were observed and counted under the fluorescence microscope. Results Both DCA and TCA could result in the increasing of average tail length of the treated cells whether they were V79 cells or hepatocytes of mice and the dose-response relationships were seen. Conclusion Both DCA and TCA can cause DNA damage of mammalian cells and this may be related to the carcinogenic mechanism. DCA and TCA belong to genetic carcinogens.

To study the mechanism ofstimulating effect of 1,25 dihydroxyvitamin D 3 on induction of neovascularization during gastric carcinogenesis induced by N methyl N′ nitro N nitrosoguanidine in rats, gastric carcinogenesis in rats was induced by administration of MNNG (150mg/L) in drinking water. Four weeks after MNNG exposure, rats were fed with diet containing 1,25(OH) 2 D 3 (2.5?g/kg, 5.0?g/kg) . Animals were killed at week 16, and 32 for the study of neovascularization by cardiovascular perfusion with carbon ink and immunohistochemical staining of vascular endothelial cell growth factor (VEGF). Expression of VEGF and microvessel density in glandular stomach of rats receiving 1,25(OH) 2 D 3 ( 5.0?g/kg) in the diet dramatically increased when compared with the rats receiving MNNG alone at week 16, and the differences were more significant at week 32. Expression of VEGF was closely correlated with microvessel density. We propose that certain dose of 1,25(OH) 2 D 3 stimulated neovascularization during gastric carcinogenesis in rats induced by MNNG partly through increasing VEGF expression.

Quercetin is the major bioflavonoid in the human diet and can be ubiquitously found in nature.Studies have suggested that quercetin has many biological activities and clinical applications such as antioxidant,anti-inflammation, anti-tumor effects. This article reviews recent advances in its anti-oxidation mechanisms, protective effects on oxidative stress-related diseases, and other applications.