1.Oxidant regulates macrophage colony-stimulating factor gene expression in RAW264.7 cells
Zhanjun PANG ; Mei ZHOU ; Yua CHEN
Chinese Journal of Pathophysiology 1986;0(01):-
AIM:To find out the effect of oxidative stress on macrophage colony-stimulating factor (M-CSF) gene expression in macrophages.METHODS: The effect of tert-butyl hydroperoxide (tbOOH) on M-CSF expression in RAW264.7 cells was investigated by RT-PCR method.RESULTS: The results showed that tbOOH (1 5?10 -4 mol/L) could induce M-CSF mRNA expression in RAW264.7 cells; and the induction became greater with increase in tbOOH concentration. Furthermore, the induction of M-CSF in RAW264.7 cells by tbOOH could be attenuated by cycloheximide or acetovanilone added in the medium.CONCLUSION: The results indicated that tbOOH could transcriptionally induce M-CSF expression in RAW264.7 cells, and de novo protein synthesis and intracellular superoxide (O 2) production might be involved in the process.
2.The molecular characteristics of heteroresistant vancomycin-intermediate Staphylococcus aureus in China
Hongbin CHEN ; Hui WANG ; Wenjia SUN ; Yudong LIU ; Minjun CHEN ; Yanrong LAI ; Jianzhong ZHANG ; Yua MA
Chinese Journal of Laboratory Medicine 2009;32(11):1223-1227
Obiective To investigate the molecular characteristics of heteroresistant vancomycinintermediate Staphylococcus aureus(hVISA)in China and analyze the differences of the molecular characteristics between hVISA and VSSA(vancomycin-susceptible Staphylococcus aureus)isolates.Methods A total of 3 15 non-repetitive MBSA were collected from the national surveillance program in China in 2007.The isolates of hVISA were confirmed by modified population analysis profile-area under the curve(PAP-AUC).The genotypes of agr and SCCmec were determined by multiplex PCR,and spa typing was performed bv PCR and DNA sequencing.The pvl gene was detected bv PCR Results The prevalence of hVISA was 9.5%(30/315).Among 315 MRSA,SCCmec Ⅲ was the most popular type,which was found in 234 isolates(234/315,74.3%),followed by SCCmec Ⅱ,which was identified in 56 isolates (56/315,17.8%).The rate of SCCmec Ⅱ in hVISA(46.7%)was significantly hisher than in VSSA (14.7%,X~2=18.93,P<0.001).The most prevalent agr type among 315 MRSA was agr 1 accounting for 73.6%(232/315).The agr 2 accounted for 18.7%(59/315),and agr 3 and agr 4 were very rare in clinical isolates.It was different in agr types between the two groups.The rate of agr 2 in hVISA(53.4%)was higher than in VSSA(15.1%).X~2 value was 26.08 and P value was less than 0.001 through X~2 test.There was a statistical significance in the result.There were 4 spa types in hVISA isolates,including t002 (13 isolates),t037(9 isolates),t030(6 isolates),and 1548(2 isolates).The pvl positive MRSA isolates were very low,accounting for 1.6%(5/315).Conclusions The prevalence of hVISA was relatively higher in China.Compared to VSSA,the majority(53.4%)of the hVISA strains were agr 2,which was obviously different from VSSA.hVISA isolates were more diverse by spa typing,
3.lncRNA SNHG11 promotes the malignant biological behaviors of NSCLC A549 cells by adsorbing miR-193a-5p
WANG Yua ; CAO Jialia ; CHEN Zhecongb ; GAO Mengyuanb ; CHEN Wenhuc
Chinese Journal of Cancer Biotherapy 2022;29(2):108-113
[Abstract] Objective: To investigate the effect of lncRNA SNHG11 on the proliferation, invasion and migration of non-small cell lung cancer (NSCLC) A549 cells and its possible mechanisms. Methods: qPCR was used to detect the levels of lncRNA SNHG11 and miR-193a-5p in human embryonic lung cells (HEL-1) and lung cancer cells (A549, H1299, and HCC827). A549 cells were transfected with SNHG11 small interfering RNA (si-SNHG11), miR-193a mimic or miR-193a inhibitor. The proliferation of A549 cells was detected by CCK-8 assay, migration and invasion of A549 cells were detected by Wound healing and Transwell assay, the protein expression of Ki67 and Cyclin D1 was determined by Western blot, and the targeting relationship between lncRNA SNHG11 and miR-193a-5p was verified by Dual-luciferase reporter experiment. Results: Compared with HEL-1 cells, the expression level of lncRNA SNHG11 was significantly increased while the expression of miR-193a-5p was decreased in lung cancer A549, H1299 and HCC827 cells (all P<0.05). Silencing lncRNA SNHG11 inhibited the proliferation, migration and invasion of A549 cells and reduced the protein expression of Ki67 and Cyclin D1 (all P<0.05). Over-expression of miR-193a-5p inhibited the proliferation, migration and invasion of A549 cells (all P<0.05). lncRNA SNHG11 could targetedly adsorb miR-193a-5p. miR-139a-5p inhibition could partially reverse the effect of silencing lncRNA SNHG11 on the proliferation, invasion and migration of A549 cells (all P<0.05). Conclusion: lncRNA SNHG11 promotes the proliferation, invasion and migration of NSCLC cells by adsorbing miR-193a-5p.