1.Clinical features of multiple trauma patients combined with spine and spinal cord injuries
Fan YANG ; Xianzhou SONG ; Xiangjun BAI ; Xiyuan ZHOU ; Jian ZHANG ; Yua CAO
Chinese Journal of Trauma 2011;27(2):152-156
Objective To analyze the clinical features of the multiple trauma patients combined with spine and spinal cord injuries.Methods A retrospective study was performed in 143 multiple trauma patients combined with spine and spinal cord injuries admitted to our department between March 2004 and March 2009.The parameters including injury cause,segment of injuries,associated injuries,complications,treatment methods and outcomes were analyzed.Results Falling and traffic accidents were the main causes for the injuries of spine and spinal cord,accounting for 53.8%(77 cases)and 38.5%(55 cases),respectively.The injured segments involved 101 lumbar vertebrae(50.8%),61 thoracic vertebrae(30.7%),29 cervical spines(14.6%)and 8 sacrococcygeal vertebrae(4.0%).The associated injuries were located at chest(163 regions,38.6%),abdomen(84 regions,19.9%),head and neck(77 regions,18.3%),extremity(65 regions,15.4%),face(17 regions,4.0%)and body surface(16 regions,3.8%).The early complications included electrolyte disturbances in 33 patients (16.8%),respiratory infection in 30(15.3%)and abdominal distention in 19(9.7%).The late complications were malnutrition in 26 patients(13.3%),amyotrophy in 23(11.7%)and deep vein thrombus in 11(5.6%).Treatment methods were operations and expectant treatments in 106 patients (74.1%)and 37(25.9%)respectively.According to American Spinal Injury Association(ASIA)scale,there were 20 patients(14.0%)at grade E before treatment and 53(37.1%)at grade E after treatment.Of all,12 patients were died of mainly multiple organ failure(MOF),cerebral hernia and malnutrition,with mortality rate of 8.39%.There showed an increase of complication and mortality rate with increase of ASIA grade(P < 0.05).Conclusions The spine and spinal cord injuries in patients with multiple trauma are mainly caused by high energy injuries and characterized by high injury severity,complex associated injuries,multiple complications,difficult management and high mortality rate.
2.lncRNA SNHG11 promotes the malignant biological behaviors of NSCLC A549 cells by adsorbing miR-193a-5p
WANG Yua ; CAO Jialia ; CHEN Zhecongb ; GAO Mengyuanb ; CHEN Wenhuc
Chinese Journal of Cancer Biotherapy 2022;29(2):108-113
[Abstract] Objective: To investigate the effect of lncRNA SNHG11 on the proliferation, invasion and migration of non-small cell lung cancer (NSCLC) A549 cells and its possible mechanisms. Methods: qPCR was used to detect the levels of lncRNA SNHG11 and miR-193a-5p in human embryonic lung cells (HEL-1) and lung cancer cells (A549, H1299, and HCC827). A549 cells were transfected with SNHG11 small interfering RNA (si-SNHG11), miR-193a mimic or miR-193a inhibitor. The proliferation of A549 cells was detected by CCK-8 assay, migration and invasion of A549 cells were detected by Wound healing and Transwell assay, the protein expression of Ki67 and Cyclin D1 was determined by Western blot, and the targeting relationship between lncRNA SNHG11 and miR-193a-5p was verified by Dual-luciferase reporter experiment. Results: Compared with HEL-1 cells, the expression level of lncRNA SNHG11 was significantly increased while the expression of miR-193a-5p was decreased in lung cancer A549, H1299 and HCC827 cells (all P<0.05). Silencing lncRNA SNHG11 inhibited the proliferation, migration and invasion of A549 cells and reduced the protein expression of Ki67 and Cyclin D1 (all P<0.05). Over-expression of miR-193a-5p inhibited the proliferation, migration and invasion of A549 cells (all P<0.05). lncRNA SNHG11 could targetedly adsorb miR-193a-5p. miR-139a-5p inhibition could partially reverse the effect of silencing lncRNA SNHG11 on the proliferation, invasion and migration of A549 cells (all P<0.05). Conclusion: lncRNA SNHG11 promotes the proliferation, invasion and migration of NSCLC cells by adsorbing miR-193a-5p.
3.Down-regulation of miR-183 targeted tumor suppressor CPEB1 enhances radiosensitivity of glioma cells
YANG Sena ; DONG Lixina ; ZHANG Yanqiua ; FU Baohonga ; CAO Liyana ; MAO Yua ; ZHANG Qinghuaia ; WANG Guangxiaa ; FU Zhanzhaoa ; WU Leib ; WANG Dongc
Chinese Journal of Cancer Biotherapy 2021;28(10):1115-1022
[摘要] 目的:探讨miR-183 对脑胶质瘤细胞放射敏感性的影响。方法:2020 年10 月至2021 年6 月,收集秦皇岛市第一
医院40 例脑胶质瘤组织标本,对T98G 细胞进行梯度剂量X 射线(0、2、4、6 Gy)照射。采用qPCR 检测miR-183、细胞质多腺
苷酸化元件结合蛋白1(cytoplasmic polyadenylation element-binding protein 1,CPEB1)在脑胶质瘤组织、T98G 细胞和经X 射线
照射的T98G 细胞中的表达量。将miR-183 inhibitor 转染T98G 细胞后下调miR-183 表达,经6 Gy X 射线垂直照射,CCK-8 法、
流式细胞术和WB 法,分别检测T98G 细胞增殖能力、细胞凋亡率及BAX 和Bcl2 蛋白表达量。Targetscan 软件预测和双荧光
素酶报告基因实验检测miR-183 与CPEB1 的靶向关系。下调CPEB1 表达后,经6 Gy X 射线照射,分别用CCK-8 法、流式细胞
术和WB 法检测T98G 细胞增殖能力、细胞凋亡率及BAX 和Bcl2 蛋白表达量。将pcDNA-CPEB1 或CPEB1 siRNA 质粒转染
T98G细胞,分别下调或过表达CPEB1 后,检测miR-183 通过CPEB1 对T98G细胞放射敏感性的影响。结果:脑胶质瘤组织和
细胞中miR-183 呈高表达,CPEB1 mRNA 呈低表达。T98G 细胞中miR-183 的表达量随着X 射线放射剂量增加而降低
(P<0.05),CPEB1 表达量随着X 射线放射剂量增加而升高(P<0.05)。6 Gy X 射线照射T98G 细胞后,下调miR-183 可降低细
胞增殖能力、增加细胞凋亡率,而过表达miR-183 则起到相反作用(P<0.05)。miR-183 靶向CPEB1 mRNA 且负调控CPEB1 表
达。下调CPEB1 表达后,经6 Gy X 射线照射可显著提高T98G 细胞增殖能力(P<0.05)、降低细胞凋亡率(P<0.05),miR-183 可
逆转CPEB1 过表达对细胞T98G 放射敏感性的促进作用(P<0.05)。结论:下调miR-183 的表达能够负调控CPEB1,从而增强
脑胶质瘤细胞的放射敏感性。