AIM: To establish the quality standard for Duhuo Jisheng Mixture (Radix Angelicae Pubescentis, Herba Taxilli, Cortex Fraxini, etc.) METHODS: Herbs in Duhuo Jisheng Mixture were identified by TLC, and osthol content was determined by TLCS. RESULTS: Rhizoma Chuanxiong; Radix Angelicae Sinensis; Radix Saposhnikoviae; Radix Gentianae Macrophyllae; Cortex Eucommiae; Herba Asari; Radix Paeoniae Alba could be determined. Osthol showed a good linear relationship at a range of 0.466 - 3.600 ?g,r= 0.999 2 . The average recovery was 95.4% . CONCLUSION:The methods are available with a reproducibility and can control the quality of this mixture.

AIM:To study the detection limits of aristolochic acid in Herba Asari preparation by HPLC. METHODS: Whether Asarum hetertropoides Fr.Schmidt ver.Mandshuricum(Maxim.) Kitag contained aristolochic acid A or not by HPLC and MS,and the difference in the reference substance and the sample solution was compared. RESULTS: Aristolochic acid A could not be found in A.hetertropoides Fr.Schmidt ver.Mandshuricum(Maxim.)Kitag by HPLC,but be found by MS. CONCLUSION:A.hetertropoides Fr.Schmidt ver.Mandshuricum (Maxim.)Kitag contained trace aristolochic acid,but it is difficult to measure aristolochic acid in Herba Asari by HPLC.The baseline noise of the sample solution can interfere with the detection limit of the trace compound compound

Objective: To study the floating and sustained release performance of Xiaojin Cinnabar Floated Sustained Release Capsules. Methods: HPMC was used to form the framwork of floated sustained release capsules containing Xiaojin Cinnabar. The content, floating time in vitro and releasing rate were studied.Results: The floating time in vitro was over 3 hours. The total released amount in 3 hours came to 60%. The conventional Xiaojin Cinnabar didn't have the floating performance. Conclusion: Xiaojin Cinnabar Floated Sustained Relegse Capsules is superior to the conventional Xiaojin cinnabar in the sustained release performance.

Adult ; Humans ; Male ; Myocarditis

Humans ; Oncogenes ; Stomach Neoplasms ; genetics

Gastrointestinal Diseases ; physiopathology ; Gastrointestinal Motility ; Humans ; Kinetics ; Postoperative Complications

Gastrointestinal Neoplasms ; pathology ; Humans ; Neoplasm Recurrence, Local ; surgery ; Postoperative Period

As a new concept, the definition of translational medicine remains obscure. The translational medicine connects the bench to bedside, and its importance would be more remarkable. The development of gastrointestinal surgery reflects the idea of translational medicine. To carry out the translational study, the gastrointestinal surgeon must learn how to find subjects from clinical problems, how to collect complete information and tissues, how to collect complete information and tissues, how to collaborate with others from different fields and how to utilize all kinds of resources. By translational studies, gastrointestinal surgeons may further improve the survival of patients with gastrointestinal tumor.
Gastrointestinal Neoplasms ; Humans ; Translational Medical Research

AIM: To compare N-LOK with ?-CD on inclusion ability for the volatile oil from Duhuo Jisheng Granula (Radix Angelicae, Pubescentis, Herba Asari, Radix Saposhni-koviae, etc). and its process. METHODS: Colloidal mill and mixer were adopted, and analysis was on use of DSC and GC/MS. RESULTS: N-LOK inclusion complex included oil ratio was twice as large as the ?-CD, GC/MS analysis showed that the main components of volatile oil before and after being covered had no significant difference. CONCLUSION: N-LOK inclusion complex included oil ratio was far higher than the ?-CD. N-LOK inclusion complex could spray to dry directly, but ?-CD inclusion complex needed refrigeration and low temperature were necessary. The method of covered with N-LOK is suitable for mass production in the traditional Chinese patent medicine in the size of manufacture.

Objective To study the expression of phosphoinositide 3 kinase (PI3-K) and its relationship with lung cell apoptosis in rats with acute lung injury induced by oleic acid. Methods 60 healthy male SD rats were divided into 5 groups randomly, with 12 rats for each group. 0.25ml/kg oleic was injected into the tail vein in 4 oleic groups in which the rats were respectively sacrificed after 1h, 2h, 4h, 6h and 24h by exsanguination. In the control group 0.25ml/kg of saline was injected intravenously and sacrificed 2h later. Arterial blood gas, left lung wet/dry ratio, lung index, protein content of BALF, and pulmonary permeability index (PPI) were determined. Fas-L expression of lung tissue was determined by immunohistochemical method and protein expression of PI3-K was determined by Western blot and immunohistochemical method. Results PaO_2 was lowered, and left lung wet/dry ratio, lung index, and protein content of BALF and PPI were increased obviously after oleic acid injection. Fas-L and PI3-K in bronchus epithelial cells, alveolar epithelial cells, pulmonary vessel endothelial cells and macrophage cells were highly expressed in the lung of ALI rats, and peak value of PI3-K appeared earlier than Fas-L. Conclusion Fas-L related apoptosis of bronchial epithelial cells, alveolar epithelial cells, pulmonary vessel endothelial cells and macrophage cells participated in the pathogenesis of earlier period of ALI through PI3-K transduction pathway.