The sera of 40 normal controls,61 cases of various malignant diseases except pancreatic cancer,53 cases of various henign diseases,and 33 cases of pancreatic cancer were examined with ELISA to determine the serum level of pancreatic cancer-associated antigen(PCAAc).Its normal value was 12.59?6.34 mg/L(x?s),and the value was 57.25?82.93 mg/L in the pancreatic cancer group,which was significantly higher than the normal value and that of the other malignant disease group(P

Animals ; Cells, Cultured ; Dioxins ; pharmacology ; Insulin-Like Growth Factor II ; genetics ; metabolism ; Osteoblasts ; drug effects ; metabolism ; RNA, Messenger ; genetics ; Rats ; Rats, Wistar

Objectives To compare the efficiency and clearance of single dose low molecular weight heparin(LMWH) as anticoagulant in hemodiafiltration and hemodialysis.Methods Twenty-two patients were enrolled in the study.A single injection of LMWH(Nadroparin calcium 7500ICUAXa) was given before hemodiafiltration and hemodialysis respectively.The anti-factor-Xa activity and the activated partial thromboplastin time(APTT) in plasma were assayed during the treatment,and the dialyzer coagulation was observed.The changes of urea and serum creatinine were measured before and after the treatment.Results The anti-factor-Xa activity of LMWH during hemodiafiltration was lower than that during hemodialysis.However,there was no significant difference in APTT between the two groups.Conclusions A single bolus injection of Nadroparin provides a simple and effective anticoagulation regimen for hemodiafiltration lasting up to four hours.

This article discussed the problems in teaching medical imaging from such aspects as teaching contents,methods,conditions and teacher cultivation on the basis of our teaching practice.

Object To optimize the process for the extraction of the original recipe used in the treatment of eczema to give a new ECZEMA SPRAY dosage form Methods The extraction process was studied by orthogonal experimental design as guided by determining the content of paeonol and baicalin in the extract Results The optimal extraction process was to reflux the original recipe with 80% ethanol twice at a bath temperature of about 90 ℃ for 1 5 and 1 0 h respectively The amount of ethanol used for each extraction was 10 and 8 times of the original recipe respectively Conclusion The above extraction process gave the most rational and satisfactory results

Cerebral Hemorrhage ; diagnosis ; Cerebral Infarction ; diagnosis ; Diagnosis, Differential ; Humans ; Medicine, Chinese Traditional

This paper proposed a rehabilitation training system with electromyography (sEMG) feedback for stroke patients based on ARM embedded system and LabVIEW. The system can achieve real-time acquisition, processing and dualview of multi-channel sEMGs and compute related sEMG parameters including iEMG, RMS, MPF and co-contraction ratio. The system was detected by clinical experiments and related inspection department. The result showed that the system is functional, interactive and in accordance with the relevant standards for medical devices so that it can fully satisfy the clinical demands. In addition, the system can help doctors to master the training state of the patient more effectively in a real-time and quantitative way that is direct to improve the training programs of stroke patients.
Electromyography ; Humans ; Neurofeedback ; Stroke Rehabilitation

Objective To assess the in vitro affinity and apoptosis-inducing effect of 131I-K237 peptide (H-His-Thr-Met-Tyr-Tyr-His-His-Tyr-Gln-His-His-Leu-OH) to LNCaP prostate cancer cell line.Methods The K237 peptide was radiolabeled with 131I by the Iodogen method.The radiolabeling efficiency and radiochemical purity after purification were then characterized by TLC in vitro.LNCaP cells were inoculated in 96-well cell plate and divided into following groups (3 duplicate wells for each group):15 kBq 131I-K237was added in the experimental group,different doses of Na131I (5,10,15 kBq) were added in 3 negative control groups,15 kBq 131I-K237 with different doses of unlabeled K237 (1,2,4,8,16 μ g/μl) were added in 3 blocking groups,and PBS was added in blank control group.The cellular binding ratios were calculated after 48 h.LNCaP cells were inoculated in 24-well cell plate and divided into 3 groups:131I-K237group,which including 3 different dose subgroups (5,10,15 kBq) ; unlabeled K237 group,which including 3 different dose subgroups (1,2,4 μg/μl) ; blank control group with 100 μl PBS.All the cells were cultured for 48 h,then optical microscopy (OM) and fluorescence microscopy (FM) were used to observe the cell morphology ; DNA gel electrophoresis was conducted and flow cytometry (FCM) was used to estimate the apoptotic rate of LNCaP cells.One-way analysis of variance and the least significant difference (LSD)-t test were used to analyze the data.Results The labeling efficiency of 131I-K237 was (73.7±3.2) ％ and the radiochemical purity was (96.7±0.6) ％ after purification.The binding ratio of experimental group was (95.8±1.5)％,whereas the ratio of negative groups with 5,10,15 kBq Na131I and PBS group was (8.2±0.4) ％,(8.3±0.6) ％,(8.6±0.5) ％ and 0,respectively.The binding ratio of 131I-K237 and LNCaP significantly declined with the increased dose of unlabeled K237 (t=4.71,P＜0.01).The apoptosis of LNCaP cells cultured with 131I-K237 was observed.Typical DNA ladder was found by DNA gel electrophoresis.The apoptotic rates of 5,10,15 kBq131I-K237 groups were (34.1±2.9)％,(37.3±3.4)％ and (41.7±3.6)％,respectively; whereas those of unlabeled K237 groups and blank control group were (10.8±1.0) ％,(12.5±2.1) ％,(13.1±2.4) ％ and (2.9±0.3) ％,respectively.There were significant differences of apoptotic rate among groups (F=76.31,P＜0.05).The difference among 5,10,15 kBq 131I-K237 groups was statistically significant (t=3.09,3.27,4.52,all P＜0.05).Conclusion 131I-K237 can bind to LNCaP cells with highly affinity and has significant apoptosis-inducing efficacy on the prostate cancer cell line.

Objective With the emerging omnipresence of arthroscopy, the plica syndrome has achieved a clinical recogni-tion as a pathological entity .This study is to investigate the clinical diagnosis and treatment of the medial plica syndrome of the knee . Methods We retrospectively analyzed 198 cases of medial plica syndrome, internal semilunar cartilage and chondromalacia patellae in the knee joints treated in our department from January 2008 to December 2011 .All the patients received physical and MRI examina-tions before admission and underwent plicaectomy, their knee function evaluated according to their Lysholm scores pre-and post-opera-tively. Results The diseased plica synovialis was completely excised in 46 cases diagnosed as simple medial plica syndrome by ar-throscopy.Forty-four of the patients were followed up for 6 to 32 (mean 26) months, and the excellence rate of treatment result was 95.5%. Conclusion Medial plica syndrome of the knee constitutes a larger proportion of knee disorders, for which arthroscopy re-mains the best diagnostic option and total excision of the diseased plica synovialis is an effective treatment .

Objective To investigate the relationship between the hs-c-reactive protein and the severity of coronary artery disease.Methods All the 67 patients underwent coronary angiography and measured risk factors,the Gensini score was used to determine the results of the coronary angiography.The t test,One-Way ANOVA and multiple linear regression analysis were used to predict hs-CRP.Results Coronary artery disease group hs-CRP levels were significantly higher than those in non-coronary artery disease group(P