Objective To improve the mammographic diagnostic level of breast ductal diseases.Methods X-ray features,operationand pathologic findings in 166 cases of breast disease were retrospectively analyzed.Results The galactography of all 166 cases showed the manifestations including 78 cases of mammoductal ectasis with ductal dilatation,smooth wall;2 cases of ductal ectasis complicated by infection with ductal dilatation,inregular coarsening wall and interstitial extravasation of contrast medium;11 cases of mammoglandular cystic lobular hyperplasia with cyetic dilatation of terminal ducts in different sizes;49 cases of intraductal papilloma with sound as ovoid fillingdefects showing as interrupted smooth bordered,"cupping" in 26 cases of intraductal papillary carcinoma or intraductal carcinoma with post dilatation ductal stenosis and sigid "amputation" absuption together with sound cauliflower or inregular filling defects.In this series of 166 cases,75 cases vice prover to be tumor(45.1%) with malignancy in 26 cases(15.7%).Conclusion Galactography is an important method for diagnosis and differential diagrosis of mammoglandular disease with nipple discharge.

Objective To investigate the effect of purified urinary IgG from patients with minimal change nephrotic syndrome and membranous nephropathy on the expression of macrophage migration inhibitory factor (MIF) in human proximal tubular epithelial cells (HK-2). Methods Proteins were precipitated from urine with (NH4)2SO4, and urinary IgG was purified by protein G column chromatography. SDS-PAGE and Western blotting were performed to analyze the urinary IgG. HK-2 cells were incubated with urinary IgG (0,0.5,1.0,2.5,5.0,10.0 mg/ml) from either minimal change nephrotic or membranous nephropathy patients. The expression of MIF mRNA was assessed by RT-PCR, and MIF protein was assessed by Western blotting. Results SDS-PAGE showed that there were four bands, which were all IgG fractions confirmed by Western blotting. The urinary IgG up-regulated the expression of MIF mRNA and protein in HK-2 cells. The expression increased in a dose-dependent manner. The expression of MIF mRNA and protein in HK-2 cells increased significantly when they were incubated with urinary IgG from membranous nephropathy patients at 1mg/ml (P

Dental Bonding ; Dental Implant-Abutment Design ; Humans ; Matrix Metalloproteinases

Objective To compare the influences of different routes of nutrition on the outcome and respiratory muscle strength of elderly patients in respiratory intensive care unit (RICU). Methods Totally 147 elderly patients in RICU were equally randomized into combined nutrition group ( combinination of parenteral nutrition and enteral nutrition), total enteral nutrition (EN) group, and total parenteral nutrition (PN) group. The changes of energy metabolism, respiratory muscle strength, and short-term outcome were observed or determined.Results Plasma albumin (ALB), hemoglobin (Hb), creatinine (Cr) levels, and nitrogen balance significantly increased in all group 7 days later ( all P ＜ 0. 01 ), while blood urea nitrogen (BUN) significantly decreased ( P ＜0.01 ). The increase of ALB, Hb, Cr levels, and nitrogen balance were significantly more remarkable than that in EN group and PN group (P＜0.05 or P＜0.01). The decrease of BUN level in the combined nutrition group was significantly more remarkable than that in EN group ( P ＜ 0. 05 ). After nutritional support, the maximum inspiratory pressure in the combined nutrition group (P =0. 021 ) and EN group (P = 0. 011 ) became significantly higher, and occlusion pressure at 0.1 second inspiration level in the combined nutrition group became significantly lower ( P =0. 025). The incidences of infectious and non-infectious complications in PN group were significantly higher than those in EN group (P = 0. 002 and 0. 017, respectively) and combined nutrition group ( P = 0. 005 and 0. 004, respectively). Gastric retention was more common in EN group than that in PN group ( P = 0. 035). The weaning time,length of RICU stay, length of hospital stay and 20-day-mortality were significantly decreased in the combined nutrition group than those in the other two groups ( all P ＜ 0. 05 ). Conclusion The effectiveness of the combined application of PN and EN is superior to PN or EN alone in improving nutritional status and respiratory muscle strength as well as in improving the short-term outcome in elderly patients in RICU..

More and more attention has been drawn on nano-materials in the application of molecular imaging and targeted cancer therapy. Quantum dots, due to their rich surface chemical properties and bright stable fluorescence, have been used as a new type of nano-probes and widely used in molecular, cellular and vivo biological imaging. In recent years, many inspiring achievements have been obtained in the study on quantum dots surface modification and biological imaging in cell and animals. In this article, current status and the latest developments of the study and the application are reviewed.

Objective To investigate the effect of lipid soluble fraction in the radix of Salvia miltiorrhiza (FSM) on conditioned place preference (CPP) in mice induced by morphine and preliminarily identify the fraction in the radix of S. miltiorrhiza. MethodsMorphine or NS was sc injected every other day to induce the obvious CPP in mice for 6 d. Before 30 min of sc injecting morphine, mice were ip administered different doses of lipid soluble fraction in the radix of S. miltiorrhiza. RP-HPLC method was used to identify the major component in the lipid soluble fraction in the radix of S. miltiorrhiza. ResultsThe staying time in morphine-paired white compartment was significantly prolonged. After treatment with lipid soluble fraction in the radix of S. miltiorrhiza (40 mg/kg, ip), the staying time in morphine-paired white compartment was significantly shortened (P

Aged ; Anthracosis ; genetics ; Case-Control Studies ; Humans ; Male ; Middle Aged ; Polymorphism, Genetic ; Transforming Growth Factor beta ; genetics ; Tumor Necrosis Factor-alpha ; genetics

Objective To provide histological evidence for clinic tendon reconstruction on severe medial collateral ligament injury in rabbits. Methods Medial collateral ligaments of two knee joints were completely incised to create own control model of MCL rupture. The operation group intimated the procedure of human being MCL reconstruction with semitendinous tendon. In the 4th,12th,16th,24th weeks, healing of MCL was observed by anatomy and the picrosirius-polarization method. Results In the 4th week, the MCL healing tissue of ex-periment group is more than control group. The average ratios of type Ⅲ collagen to Ⅰ, Ⅲ collagen in the experiment group [(0.263±0.075), (0.235±0.041), (0.210±0.045), (0.197±0.029)] were significantly lower than those in control group [(0.310±0.072), (0.286±0.045), (0.261±0.046), (0.236±0.043) ] in the 4th, 12th, 16th, 24th weeks, respectively (P < 0.05). Conclusions The semitendinons tendon reconstruction can improve MCL healing in early and middle period after injury.

Objective The clinical results of leukemia improved significantly due to the application of arsenic trioxide(As 2 O 3 ).This compound was used in inducing apoptosis on osteosarcoma cell lines and its mech anism was explored.Methods Human osteosarcoma MG-63cell line was cultured and treated with0.25,0.5,1,2,4and8?mol/L As 2 O 3 .The cell line was measured at24,48,72,96and120h after As 2 O 3 infiltration.MTT assay,phase contrast light microscopy,fluorescence staining,TUNEL,flow cytometry anal-ysis and RT -PCR were used to investigate the inducing apoptosis and inhibitative effect of As 2 O 3 on os-teosarcoma MG-63cell line.Results As 2 O 3 could inhibit the reproduction of osteosarcoma cell line MG-63significantly,the inhibitive rates of MG-63cell line treated with As 2 O 3 over1?mol/L reached70%.Un -der phase contrast light microscopy and fluorescence staining,osteosarcoma cells cultured with As 2 O 3 pre sent -ed typical apoptotic changes.The majority of cells cultured with As 2 O 3 were positive with TUNEL.Flow cy tometry analysis showed a time-dose dependent apoptosis rate and a remarkable G 2 /M phase arrest.The ex pression of c-myc was decreased in MG-63cells treated with As 2 O 3 .Conclusion As 2 O 3 is able to in duce osteosarcoma apoptosis effectively.The expression of decreasing c-myc is an important mechanism in induc-ing osteosarcoma MG-63cells apoptosis.

Objective To investigate the lipidemia of patients with coronary artery disease(CAD)in China.Methods The survey engaged 52 centers in 7 cities in China recruiting patients with coronary artery disease in a hospital-based setting.Each center was asked to recruit all hospitalized patients with CHD from 1-Jun-2005 to 31-Sep-2005.The study population consisted of 3513 patients with CAD.Results Of the 3513 patients,the prevalence of the lipidemia was 79.7%.And the prevalence of the lipidemia in